Abstract:
:Increased numbers of eosinophils and increased concentrations of plasma proteins have been found in the airways of patients with mild asthma. We used an intact guinea pig trachea model to investigate the role of eosinophil peroxidase (EPO) in altering the function of the airway epithelial barrier. EPO in the presence of hydrogen peroxide (H(2)O(2)) and bromide (Br(-)) catalyzes the production of hypobromous acid (HOBr), which is felt to have a toxic effect on airway epithelial cells. An intact guinea pig trachea was mounted on an apparatus in a way that would allow the tracheal epithelium to be exposed to different solutions. Following these exposures, a test solution containing (14)C-sucrose (S), (3)H-inulin (I), and FITC-dextran-20 (D) was placed in the tracheal lumen and positioned in the center of the segment for 90 minutes. Flux of these molecules across the epithelial barrier into a bath was measured, and the permeability (P) was calculated for each molecule to quantify epithelial barrier function. Light and electron micrographic studies were performed to assess cellular damage. We found that there was a dose response to EPO (in the presence of fixed amounts of H(2)(O)(2) and Br(-)). EPO at 7.3 x 10(-7) M caused no increase in P over controls (Ringer's solution alone) for S, I, or D (P> 0.05), whereas EPO at 2.7 x 10(-6) M caused a significant increase in P over controls (P = 0.008) for all test molecules. Light and electron micrographs of the latter tracheas showed no evidence of microscopic changes despite the increased P. Further testing verified that the increase in permeability was caused by the EPO catalyzed reaction and not the individual substrates themselves, and that the reaction was inhibited by a peroxidase inhibitor. We conclude that EPO can alter the barrier function of the airway epithelium before gross cellular damage becomes visible. We hypothesize that changes in the tight junctions are responsible for the alteration in the barrier function of the airway epithelium and that this may play an important role in the pathophysiology of mild asthma.
journal_name
Pediatr Pulmonoljournal_title
Pediatric pulmonologyauthors
Brottman GM,Regelmann WE,Slungaard A,Wangensteen ODdoi
10.1002/(SICI)1099-0496(199603)21:3<159::AID-PPUL2subject
Has Abstractpub_date
1996-03-01 00:00:00pages
159-66issue
3eissn
8755-6863issn
1099-0496pii
10.1002/(SICI)1099-0496(199603)21:3<159::AID-PPUL2journal_volume
21pub_type
杂志文章abstract::Thirteen children with asthma were treated with cumulative doses of terbutaline delivered as a pressurized aerosol and from a new multidose powder inhaler (Turbuhaler) in a randomized cross-over dose-response study. The cumulative dose of terbutaline was 2 mg on each study day. All children used a correct aerosol inha...
journal_title:Pediatric pulmonology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ppul.1950070211
更新日期:1989-01-01 00:00:00
abstract::The prevalence of respiratory syncytial virus (RSV) infection was studied during three consecutive annual outbreaks (1983-1986) in SR Croatia, Yugoslavia. A total of 1,238 subjects were examined, using RSV isolation and immunofluorescent (DTFA) methods, with 1,042 showing the signs of respiratory infection and 207 of ...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950030505
更新日期:1987-09-01 00:00:00
abstract:BACKGROUND:Quantifying the chest wall is useful in documenting thoracoabdominal synchrony during the neonatal period. Subjective measures are often used rather than gold-standard methods due to their practicality in clinical practice. The aim of the present study is to compare the reliability between a newly proposed m...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24709
更新日期:2020-05-01 00:00:00
abstract::The pediatric pneumogram is a frequently used tool in the diagnosis and management of apnea during infancy. We analyzed 287 pneumographic recordings from 123 full-term infants (63 males) obtained during the first 12 months of life to establish normative values for apnea, periodic breathing, and bradycardia. The result...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950010409
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:There is minimal literature available on the long-term outcome of pediatric non-cystic fibrosis (CF) bronchiectasis. AIM:To document 5-year outcomes of children with chest computerized tomography (CT) scan diagnosed bronchiectasis from a tertiary New Zealand (NZ) respiratory clinic. METHODS:Review of a cli...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.21331
更新日期:2011-02-01 00:00:00
abstract::Four patients with severe cystic fibrosis lung disease, anorexia and weight loss, received Megestrol Acetate (MA), as an appetite stimulant. The initial dose was 400-800 mg daily and was continued for 6-15 months. Appetite was improved, with significant weight gain in all patients and an increase in their weight for a...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0496(199911)28:5<380::aid-ppul1
更新日期:1999-11-01 00:00:00
abstract::Our objective was to determine whether a simple method of maintaining positive pressure ventilation during nonbronchoscopic bronchoalveolar lavage (NB-BAL) would successfully reduce the incidence and/or severity of desaturation events. Our design was a clinical trial with historical controls. Seventy ventilated pediat...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.20082
更新日期:2004-09-01 00:00:00
abstract:INTRODUCTION:Interpregnancy interval (IPI) is defined as the period between a live birth and the conception of a subsequent fetus. Both short (IPI < 6 months) and long IPI (IPI > 60 months) have been shown to increase the risk for adverse perinatal outcomes, some of which, are known risk factors for obstructive sleep a...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25240
更新日期:2020-12-31 00:00:00
abstract::Childhood interstitial lung disease (chILD) comprises a wide heterogeneous group of rare parenchymal lung disorders associated with substantial morbidity and mortality. Pulmonary hypertension is a common comorbidity in adults with interstitial lung disease (ILD) and associated with poor survival. We aimed to systemati...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,评审
doi:10.1002/ppul.23632
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVES:To determine 1) what proportion of abstracts of randomized controlled trials (RCTs) presented at international conferences on cystic fibrosis (CF) are published as full reports, 2) time to publication, and 3) factors that might delay or prevent publication. METHODS:At the end of 1995, the Cochrane CF Group'...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/(sici)1099-0496(199808)26:2<101::aid-ppul5
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND/OBJECTIVE:Our objective was to obtain follow-up pulmonary function testing and assessment of clinical respiratory outcomes, at 1-2 years, in preterm infants whose mothers were randomized to a single rescue course of antenatal steroids (AS) versus placebo. METHODS:Follow-up of a randomized, double-blinded tr...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,随机对照试验
doi:10.1002/ppul.23711
更新日期:2017-09-01 00:00:00
abstract::The raised lung volume technique is increasingly used to measure forced expiratory maneuvers in infants. However, there is no consensus regarding the optimal airway inflation pressure (P(inf)) required for such maneuvers, or the influence of small changes in P(inf) within and between infants. The aim of this study was...
journal_title:Pediatric pulmonology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/ppul.10060
更新日期:2002-02-01 00:00:00
abstract::This study sought to determine if a clinical pathway developed and executed by specialists in pediatric asthma would reduce hospital costs and length of stay (LOS). The study design was a retrospective, nonrandomized, controlled trial. Subjects were children aged 2-18 years (N = 1,004) with a history of recurrent whee...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1110
更新日期:2001-09-01 00:00:00
abstract::For the last thirty years, oral chloral hydrate has been used for sedation of infants for lung function testing. Recently, however, availability of chloral hydrate became severely limited in the United States after two manufacturers discontinued manufacturing in 2012. Due to these limitations and the recent and ongoin...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23100
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:The study was designed to assess the use of newer sidestream microstream end tidal carbon dioxide (ETCO(2) ) device in predicting blood carbon dioxide (PCO(2) ) measurements in very low birth weight (VLBW = birth weight <1,500 g) and non-VLBW NICU neonates. STUDY DESIGN:Sidestream microstream ETCO(2) detecto...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.22593
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Magnetic Resonance Imaging (MRI) techniques to image the larynx have evolved rapidly into a promising and safe imaging modality, without need for sedation or ionizing radiation. MRI is therefore of great interest to image pediatric laryngeal diseases. Our aim was to review MRI developments relevant for the p...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24250
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:a: To evaluate the long-term outcome of parapneumonic effusions (PPE) in children regarding lung function and exercise tolerance, (b) to investigate the role of bronchial asthma in the outcome of PPE. METHODS:The design of the study included 51 children with PPE, at least 2 years after the initial infection...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23054
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:Several studies have shown that exposure to air pollutants affects lung growth and development and can result in poor respiratory health in early life. METHODS:We included a subsample of 772 Mexican preschoolers whose mothers participated in a Prenatal Omega-3 fatty acid Supplements, GRowth, And Development...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24889
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Simulated exhaled nitric oxide (eNO) depends on ventilatory settings used in different experimental conditions. OBJECTIVES:To normalize the simulated minute exhaled nitric oxide according to different ventilatory settings. WORKING HYPOTHESIS:Different ventilatory settings influence the concentrations of ex...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.20893
更新日期:2008-12-01 00:00:00
abstract::The incidence of empyema complicating community-acquired pneumonia is increasing and causes significant childhood morbidity. Pneumococcal infection remains the most common isolated cause in developed countries, with Staphylococcus aureus the predominant pathogen in the developing world. Newer molecular techniques util...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,评审
doi:10.1002/ppul.20251
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND:Asthma and obstructive sleep apnea (OSA) in children share multiple epidemiological risk factors and the prevalence of snoring is higher in asthmatic children, suggesting that the latter may be at increased risk for OSA. Since both asthma and OSA are inflammatory disorders, we hypothesized that polysomnograp...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.21451
更新日期:2011-09-01 00:00:00
abstract::We measured then compared the dynamic lung mechanics of spontaneous breaths and mechanical breaths in 9 mechanically ventilated neonates with hyaline membrane disease. All were receiving intermittent mandatory ventilation. All breathed spontaneously between ventilator breaths. Tidal volume, transpulmonary pressure, dy...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.1950080403
更新日期:1990-01-01 00:00:00
abstract::Pneumatoceles and bronchiectasis are secondary complications of respiratory infections in patients with hyper IgE syndrome (HIgES). We report on a patient with HIgES and recurrent pneumonias since 1 year of age, with progression to pneumatoceles and bronchiectasis and fatal outcome, with disseminated infection and sus...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.22532
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Peripheral muscle dysfunction is increasingly recognized as complicating respiratory disease, but this is difficult to measure non-invasively. RESEARCH QUESTION:Can skeletal muscle function and efficiency be measured during exercise non-invasively using respiratory mass spectrometry (RMS); and is the know...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.20899
更新日期:2009-03-01 00:00:00
abstract::In children unable to perform reliable spirometry, the interrupter resistance (R(int) ) technique for assessing respiratory resistance is easy to perform. However, few data are available on the possibility to use R(int) as a surrogate for spirometry. We aimed at comparing R(int) and spirometry at baseline and after br...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.22526
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:To prospectively assess longitudinal lung function in children with sickle cell disease (SCD). WORKING HYPOTHESIS:Lung function in SCD children deteriorates with increasing age and the decline is more marked in younger children who have recently suffered ACS episodes. STUDY DESIGN:Two prospective longitudi...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.23367
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:Since preventive therapies for bronchopulmonary dysplasia (BPD) are limited we treated preterm infants with azithromycin to decrease the incidence of BPD. METHODS:Infants less than 1,250 g birth weight were randomized to azithromycin or placebo within 12 hr of beginning mechanical ventilation and within 72 h...
journal_title:Pediatric pulmonology
pub_type: 杂志文章,随机对照试验
doi:10.1002/ppul.21352
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Discussions on the diagnostic and management of acquired pediatric lung pathology are usually published by large tertiary children's hospitals. It is likely that much of this pathology is actually seen and managed in nonacademic practices. METHODS:A 10-year retrospective review of patients under 18-years of...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.25254
更新日期:2021-01-02 00:00:00
abstract:INTRODUCTION:Acute bronchiolitis is a major cause of acute respiratory distress in infants. The soluble receptor for advanced glycation end-products (sRAGE) is a biomarker of pulmonary damage processes, with a diagnostic and a prognostic value in acute respiratory distress syndrome (ARDS). The RAGE pathway is also impl...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.24141
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Lung involvement in children with Niemann-Pick disease has rarely been studied systematically. OBJECTIVE:To assess the involvement of the lung and the value of bronchoalveolar lavage in children with Niemann-Pick diseases. DESIGN:Retrospective analysis of patient records. PATIENTS:Thirteen patients, with ...
journal_title:Pediatric pulmonology
pub_type: 杂志文章
doi:10.1002/ppul.20725
更新日期:2007-12-01 00:00:00