H3 Cys-110 is in close proximity to the C-terminal regions of H2B and H4 in a nucleosome core with an altered internal arrangement of histones.

abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...

journal_title：Biochemistry

authors： Ahmed SA,Martin B,Miles EW

更新日期：1986-07-29 00:00:00

abstract：:Calmodulin, the Ca(2+)-dependent activator of many cellular processes, contains two well-defined structural domains, each of which binds two Ca(2+) ions. In its Ca(2+)-free (apo) form, it provides an attractive model for studying mechanisms of protein unfolding, exhibiting two separable, reversible processes, indicati...

journal_title：Biochemistry

authors： Biekofsky RR,Martin SR,McCormick JE,Masino L,Fefeu S,Bayley PM,Feeney J

更新日期：2002-05-28 00:00:00

abstract：:A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...

journal_title：Biochemistry

authors： Shriver J,Edmondson S

更新日期：1993-02-16 00:00:00

abstract：:The effects of two oppositely charged pyrene derivatives, 1-pyrenebutyrylcholine (PBC) and 1-pyrenebutyric acid (PBA), on red blood cell shape have been examined. Both compounds convert normal biconcave erythrocytes into echinocytes. However, with extended incubation time at elevated temperature, the morphology of PBC...

journal_title：Biochemistry

authors： Matayoshi ED

更新日期：1980-07-22 00:00:00

abstract：:Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G

更新日期：1982-10-26 00:00:00

abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

journal_title：Biochemistry

authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

更新日期：1992-05-19 00:00:00

abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

journal_title：Biochemistry

authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

更新日期：2002-11-05 00:00:00

abstract：:Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...

journal_title：Biochemistry

authors： Thompson JE,Basarab GS,Andersson A,Lindqvist Y,Jordan DB

更新日期：1997-02-18 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...

journal_title：Biochemistry

authors： Hicks SN,Smiley RD,Hamilton JB,Howell EE

更新日期：2003-09-16 00:00:00

abstract：:Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...

journal_title：Biochemistry

authors： Green PC,Latshaw SP,Ladror US,Kemp RG

更新日期：1992-05-26 00:00:00

abstract：:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...

journal_title：Biochemistry

authors： Deslauriers R,Komoroski RA,Levy GC,Seely JH,Smith IC

更新日期：1976-10-19 00:00:00

abstract：:Fluorescent and photoactivatable fluorescent derivatives of tetrodotoxin (TTX) have been synthesized. N-Methylanthraniloylglycine hydrazide, anthraniloyl hydrazide, and 2-azidoanthraniloylglycine hydrazide were coupled to the carbonyl at C6 of oxidized tetrodotoxin to form stable fluorescent hydrazones. The C6 ketone ...

journal_title：Biochemistry

authors： Angelides KJ

更新日期：1981-07-07 00:00:00

abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...

journal_title：Biochemistry

authors： Martinez KL,Corringer PJ,Edelstein SJ,Changeux JP,Mérola F

更新日期：2000-06-13 00:00:00

abstract：:A novel liposomal method permits studies of Ca movements across the bilayers of multilamellar vesicles (MLV) which had entrapped the Ca-dependent, fluorescent indicator dye Fura 2. Ionomycin-mediated Ca translocation across MLV of phosphatidylcholine (PC)/dicetyl phosphate (DCP), 9:1, obeyed simple first-order kinetic...

journal_title：Biochemistry

authors： Blau L,Weissmann G

更新日期：1988-07-26 00:00:00

abstract：:The reactions of nitric oxide (NO) with the turnover intermediates of cytochrome c oxidase were investigated by combining amperometric and spectroscopic techniques. We show that the complex of nitrite with the oxidized enzyme (O) is obtained by reaction of both the "peroxy" (P) and "ferryl" (F) intermediates with stoi...

journal_title：Biochemistry

authors： Giuffrè A,Barone MC,Mastronicola D,D'Itri E,Sarti P,Brunori M

更新日期：2000-12-19 00:00:00

abstract：:A survey of RuvB protein-mediated ATP hydrolysis yields the following observations. (1) The RuvB protein exhibits a DNA-independent ATPase activity with a turnover number (based on a RuvB monomer) approaching 6 min-1 and a Km of 154 microM. Single-stranded DNA and linear duplex DNA have small but significant effects o...

journal_title：Biochemistry

authors： Marrione PE,Cox MM

更新日期：1995-08-01 00:00:00

abstract：:The SfiI restriction enzyme binds to DNA as a tetramer holding two usually distant DNA recognition sites together before cleavage of the four DNA strands. To elucidate structural properties of the SfiI-DNA complex, atomic force microscopy (AFM) imaging of the complexes under noncleaving conditions (Ca2+ instead of Mg2...

journal_title：Biochemistry

authors： Lushnikov AY,Potaman VN,Oussatcheva EA,Sinden RR,Lyubchenko YL

更新日期：2006-01-10 00:00:00

abstract：:Calmodulin (CaM) is a ubiquitous protein in nature and plays a regulatory role in numerous biological processes, including the upregulation of nitric oxide (NO) synthesis in vivo. Several peptides that prevent NO production by interacting with CaM have been isolated in the cutaneous secretions of Australian amphibians...

journal_title：Biochemistry

authors： Calabrese AN,Bowie JH,Pukala TL

更新日期：2015-01-20 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...

journal_title：Biochemistry

authors： Seidman CE,Hess HJ,Homcy CJ,Graham RM

更新日期：1984-07-31 00:00:00

abstract：:Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...

journal_title：Biochemistry

authors： Ding XX,Coon MJ

更新日期：1988-11-01 00:00:00

abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...

journal_title：Biochemistry

authors： Tao L,English AM

更新日期：2004-04-06 00:00:00

abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...

journal_title：Biochemistry

authors： Du D,Ju H,Zhang X,Chen J,Cai J,Chen H

更新日期：2005-08-30 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00

abstract：:The present study uses CO as a surrogate for oxygen to probe how substrate binding triggers oxygen activation in peptidylglycine monooygenase (PHM). Infrared stretching frequencies (ν(C ≡ O)) of the carbonyl (CO) adducts of copper proteins are sensitive markers of Cu(I) coordination and are useful in probing oxygen re...

journal_title：Biochemistry

authors： Kline CD,Blackburn NJ

更新日期：2016-12-06 00:00:00

abstract：:The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulf...

journal_title：Biochemistry

authors： Rasmussen T,Scheide D,Brors B,Kintscher L,Weiss H,Friedrich T

更新日期：2001-05-22 00:00:00

abstract：:Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...

journal_title：Biochemistry

authors： Rusinova E,Ross JB,Laue TM,Sowers LC,Senear DF

更新日期：1997-10-21 00:00:00

abstract：:We have investigated the interaction of a number of synthetic 20-residue peptides, corresponding to the HA2 N-terminus of the influenza virus hemagglutinin (X31 strain), with phospholipid vesicles and monolayers. Besides the wild-type sequence, two peptides were studied with mutations corresponding to those previously...

journal_title：Biochemistry

authors： Rafalski M,Ortiz A,Rockwell A,van Ginkel LC,Lear JD,DeGrado WF,Wilschut J

更新日期：1991-10-22 00:00:00

abstract：:The ligand binding relationship between the acetylcholine transporter (AcChT) and the vesamicol receptor (VR) and the kinetics of active transport were studied in synaptic vesicles purified from the Torpedo electric organ using analogues of AcCh and vesamicol. Methoxyvesamicol, which should exhibit better equilibratio...

journal_title：Biochemistry

authors： Bahr BA,Clarkson ED,Rogers GA,Noremberg K,Parsons SM

更新日期：1992-06-30 00:00:00

abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...

journal_title：Biochemistry

authors： Bender SL,Widlanski T,Knowles JR

更新日期：1989-09-19 00:00:00