Abstract:
:The present study uses CO as a surrogate for oxygen to probe how substrate binding triggers oxygen activation in peptidylglycine monooygenase (PHM). Infrared stretching frequencies (ν(C ≡ O)) of the carbonyl (CO) adducts of copper proteins are sensitive markers of Cu(I) coordination and are useful in probing oxygen reactivity because the electronic properties of O2 and CO are similar. The carbonyl chemistry has been explored using PHM WT and a number of active site variants in the absence and presence of peptidyl substrates. We have determined that upon carbonylation (i) a major CO band at 2092 cm-1 and a second minor CO band at 2063 cm-1 are observed in the absence of peptide substrate Ac-YVG; (ii) the presence of peptide substrate amplifies the minor CO band and causes it to partially interconvert with the CO band at 2092 cm-1; (iii) the substrate-induced CO band is associated with a second conformer at CuM; and (iv) the CuH-site mutants, which are inactive, fail to generate any substrate-induced CO bands. The total intensity of both bands is constant, suggesting that the Cu(I)M-site partitions between the two carbonylated enzyme states. Together, these data provide evidence for two conformers at CuM, one of which is induced by binding of the peptide substrate with the implication that this represents the conformation that also allows binding and activation of O2.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kline CD,Blackburn NJdoi
10.1021/acs.biochem.6b00845subject
Has Abstractpub_date
2016-12-06 00:00:00pages
6652-6661issue
48eissn
0006-2960issn
1520-4995journal_volume
55pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8003704
更新日期:2008-07-22 00:00:00
abstract::Atomic resolution ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi0517133 更新日期:2006-02-21 00:00:00
abstract::The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700384t
更新日期:2007-07-03 00:00:00
abstract::One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485326
更新日期:2005-01-18 00:00:00
abstract::The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900038p
更新日期:2009-03-24 00:00:00
abstract::We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960034c
更新日期:1996-05-21 00:00:00
abstract::The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990185q
更新日期:1999-05-11 00:00:00
abstract::The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992610l
更新日期:2000-08-29 00:00:00
abstract::In efforts to understand the mechanisms of many nucleic acid enzymes, the first site-directed mutations are made at conserved acidic active residues. Almost without exception, the low or null activities of the resulting variants are attributed to the importance of the acidic residue(s) to the ligation of required meta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000337d
更新日期:2000-09-05 00:00:00
abstract::Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00667a007
更新日期:1976-11-02 00:00:00
abstract::Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00391a033
更新日期:1987-08-25 00:00:00
abstract::A series of peptide fragments of sperm whale myoglobin, corresponding to segments of the region between the G- and H-helices of the protein, have been synthesized and their conformational preferences investigated using circular dichroism and nuclear magnetic resonance spectroscopy in aqueous solution and in solvent mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00076a007
更新日期:1993-06-29 00:00:00
abstract::The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00473a009
更新日期:1990-05-29 00:00:00
abstract::D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201378c
更新日期:2011-11-22 00:00:00
abstract::X-ray diffraction measurements show that in contrast to the purple membrane, the bacteriorhodopsin molecules are not organized in a hexagonal lattice in the deionized blue membrane. Addition of Ca2+ restores both the purple color and the normal (63 A) hexagonal protein lattice. In the blue state, the circular dichrois...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00449a031
更新日期:1989-11-14 00:00:00
abstract::Ultrafast laser spectroscopy techniques are used to measure the low-frequency vibrational coherence spectra and nitric oxide rebinding kinetics of Caldariomyces fumago chloroperoxidase (CPO). Comparisons of the CPO coherence spectra with those of other heme species are made to gauge the protein-specific nature of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7025485
更新日期:2008-05-06 00:00:00
abstract::Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972612a
更新日期:1998-04-07 00:00:00
abstract::Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00026
更新日期:2015-06-30 00:00:00
abstract::Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a001
更新日期:1983-05-10 00:00:00
abstract::ROS-GC represents a membrane guanylate cyclase subfamily whose distinctive feature is that it transduces diverse intracellularly generated Ca(2+) signals into the production of the second messenger cyclic GMP. An intriguing feature of the first subfamily member, ROS-GC1, is that it is both stimulated and inhibited by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990851n
更新日期:1999-09-28 00:00:00
abstract::Recycling of yeast fumarase to permit repetition of its reaction chemistry requires two proton transfers and two conformational changes, in pathways that are different in detail but thematically similar in the two directions. In the malate --> fumarate direction, simple anions such as acetate accelerate the fumarate-o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9821521
更新日期:1998-12-22 00:00:00
abstract::The 2.15 A resolution crystal structure of arginase from Plasmodium falciparum, the parasite that causes cerebral malaria, is reported in complex with the boronic acid inhibitor 2(S)-amino-6-boronohexanoic acid (ABH) (K(d) = 11 microM). This is the first crystal structure of a parasitic arginase. Various protein const...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100390z
更新日期:2010-07-06 00:00:00
abstract::We have de novo designed a heterodimeric coiled-coil formed by two peptides as a capture/delivery system that can be used in applications such as affinity tag purification, immobilization in biosensors, etc. The two strands are designated as K coil (KVSALKE heptad sequence) and E coil (EVSALEK heptad sequence), where ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0268450
更新日期:2003-02-18 00:00:00
abstract::The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00590a031
更新日期:1979-11-13 00:00:00
abstract::ISU-type proteins mediate cluster transfer to apo protein targets. Rate constants have been determined for cluster transfer from ISU to apo Fd for both Homo sapiens and Schizosaccharomyces pombe proteins, and cross reactions have also been examined. Substitution of a key aspartate residue of ISU is found to decrease t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0256781
更新日期:2002-07-16 00:00:00
abstract::Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7019386
更新日期:2008-04-22 00:00:00
abstract::Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00420a003
更新日期:1988-10-04 00:00:00
abstract::The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9617522
更新日期:1997-05-06 00:00:00
abstract::Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct cotranslational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a two-step protocol in w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00488
更新日期:2016-08-02 00:00:00
abstract::Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992152g
更新日期:2000-07-11 00:00:00