Ump1p is required for proper maturation of the 20S proteasome and becomes its substrate upon completion of the assembly.


:We report the discovery of a short-lived chaperone that is required for the correct maturation of the eukaryotic 20S proteasome and is destroyed at a specific stage of the assembly process. The S. cerevisiae Ump1p protein is a component of proteasome precursor complexes containing unprocessed beta subunits but is not detected in the mature 20S proteasome. Upon the association of two precursor complexes, Ump1p is encased and is rapidly degraded after the proteolytic sites in the interior of the nascent proteasome are activated. Cells lacking Ump1p exhibit a lack of coordination between the processing of beta subunits and proteasome assembly, resulting in functionally impaired proteasomes. We also show that the propeptide of the Pre2p/Doa3p beta subunit is required for Ump1p's function in proteasome maturation.






Ramos PC,Höckendorff J,Johnson ES,Varshavsky A,Dohmen RJ




Has Abstract


1998-02-20 00:00:00
















  • Structure of the pericellular matrix: association of heparan and chondroitin sulfates with fibronectin-procollagen fibers.

    abstract::Immunofluorescent staining of a pericellular matrix produced by cultured human embryonic skin fibroblasts showed a codistribution among fibronectin, heparan sulfate proteoglycans and part of the chondroitin sulfate in a fibrillar network. Isolated matrix in an "intact" form could be scraped off the dish after detergen...


    pub_type: 杂志文章


    authors: Hedman K,Johansson S,Vartio T,Kjellén L,Vaheri A,Höök M

    更新日期:1982-03-01 00:00:00

  • Immunosurveillance by hematopoietic progenitor cells trafficking through blood, lymph, and peripheral tissues.

    abstract::Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse thoracic duct (TD) lymph contains HSPCs that possess short- and lo...


    pub_type: 杂志文章


    authors: Massberg S,Schaerli P,Knezevic-Maramica I,Köllnberger M,Tubo N,Moseman EA,Huff IV,Junt T,Wagers AJ,Mazo IB,von Andrian UH

    更新日期:2007-11-30 00:00:00

  • Optimal Decoding of Cellular Identities in a Genetic Network.

    abstract::In developing organisms, spatially prescribed cell identities are thought to be determined by the expression levels of multiple genes. Quantitative tests of this idea, however, require a theoretical framework capable of exposing the rules and precision of cell specification over developmental time. We use the gap gene...


    pub_type: 杂志文章


    authors: Petkova MD,Tkačik G,Bialek W,Wieschaus EF,Gregor T

    更新日期:2019-02-07 00:00:00

  • Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism.

    abstract::The broad expression of the insulin receptor suggests that the spectrum of insulin function has not been fully described. A cell type expressing this receptor is the osteoblast, a bone-specific cell favoring glucose metabolism through a hormone, osteocalcin, that becomes active once uncarboxylated. We show here that i...


    pub_type: 杂志文章


    authors: Ferron M,Wei J,Yoshizawa T,Del Fattore A,DePinho RA,Teti A,Ducy P,Karsenty G

    更新日期:2010-07-23 00:00:00

  • TGF-beta signaling specifies axons during brain development.

    abstract::In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...


    pub_type: 杂志文章


    authors: Yi JJ,Barnes AP,Hand R,Polleux F,Ehlers MD

    更新日期:2010-07-09 00:00:00

  • Molecular genetics of the achaete-scute gene complex of D. melanogaster.

    abstract::The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by lon...


    pub_type: 杂志文章


    authors: Campuzano S,Carramolino L,Cabrera CV,Ruíz-Gómez M,Villares R,Boronat A,Modolell J

    更新日期:1985-02-01 00:00:00

  • Gut Microbiota Orchestrates Energy Homeostasis during Cold.

    abstract::Microbial functions in the host physiology are a result of the microbiota-host co-evolution. We show that cold exposure leads to marked shift of the microbiota composition, referred to as cold microbiota. Transplantation of the cold microbiota to germ-free mice is sufficient to increase insulin sensitivity of the host...


    pub_type: 杂志文章


    authors: Chevalier C,Stojanović O,Colin DJ,Suarez-Zamorano N,Tarallo V,Veyrat-Durebex C,Rigo D,Fabbiano S,Stevanović A,Hagemann S,Montet X,Seimbille Y,Zamboni N,Hapfelmeier S,Trajkovski M

    更新日期:2015-12-03 00:00:00

  • CNS myelin and sertoli cell tight junction strands are absent in Osp/claudin-11 null mice.

    abstract::Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified transmembrane protein found in CNS myelin and testis with unknown function. Herein we demonstrate that Osp null mice exhibit both neurological and reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is conspicuous, and male...


    pub_type: 杂志文章


    authors: Gow A,Southwood CM,Li JS,Pariali M,Riordan GP,Brodie SE,Danias J,Bronstein JM,Kachar B,Lazzarini RA

    更新日期:1999-12-10 00:00:00

  • Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.

    abstract::The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 1...


    pub_type: 杂志文章


    authors: Cao Y,Su B,Guo X,Sun W,Deng Y,Bao L,Zhu Q,Zhang X,Zheng Y,Geng C,Chai X,He R,Li X,Lv Q,Zhu H,Deng W,Xu Y,Wang Y,Qiao L,Tan Y,Song L,Wang G,Du X,Gao N,Liu J,Xiao J,Su XD,Du Z,Feng Y,Qin C,Qin C

    更新日期:2020-07-09 00:00:00

  • Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses.

    abstract::Antibodies capable of neutralizing divergent influenza A viruses could form the basis of a universal vaccine. Here, from subjects enrolled in an H5N1 DNA/MIV-prime-boost influenza vaccine trial, we sorted hemagglutinin cross-reactive memory B cells and identified three antibody classes, each capable of neutralizing di...


    pub_type: 杂志文章


    authors: Joyce MG,Wheatley AK,Thomas PV,Chuang GY,Soto C,Bailer RT,Druz A,Georgiev IS,Gillespie RA,Kanekiyo M,Kong WP,Leung K,Narpala SN,Prabhakaran MS,Yang ES,Zhang B,Zhang Y,Asokan M,Boyington JC,Bylund T,Darko S,Lees

    更新日期:2016-07-28 00:00:00

  • Generation of Functional Human 3D Cortico-Motor Assembloids.

    abstract::Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here...


    pub_type: 杂志文章


    authors: Andersen J,Revah O,Miura Y,Thom N,Amin ND,Kelley KW,Singh M,Chen X,Thete MV,Walczak EM,Vogel H,Fan HC,Paşca SP

    更新日期:2020-12-23 00:00:00

  • Nonreciprocal exchange between alleles of the yeast mitochondrial 21S rRNA gene: kinetics and the involvement of a double-strand break.

    abstract::A 1.1 kb intron containing an open reading frame (ORF) in one allele (omega+) of the yeast mitochondrial 21S rRNA gene is nearly quantitatively inserted in crosses into a 21S rRNA allele lacking that intron (omega-). We have determined that this nonreciprocal exchange initiates soon after cells fuse to form zygotes an...


    pub_type: 杂志文章


    authors: Zinn AR,Butow RA

    更新日期:1985-04-01 00:00:00

  • Extracellular M. tuberculosis DNA targets bacteria for autophagy by activating the host DNA-sensing pathway.

    abstract::Eukaryotic cells sterilize the cytosol by using autophagy to route invading bacterial pathogens to the lysosome. During macrophage infection with Mycobacterium tuberculosis, a vacuolar pathogen, exogenous induction of autophagy can limit replication, but the mechanism of autophagy targeting and its role in natural inf...


    pub_type: 杂志文章


    authors: Watson RO,Manzanillo PS,Cox JS

    更新日期:2012-08-17 00:00:00

  • Alternative splicing accounts for the four forms of myelin basic protein.

    abstract::We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...


    pub_type: 杂志文章


    authors: de Ferra F,Engh H,Hudson L,Kamholz J,Puckett C,Molineaux S,Lazzarini RA

    更新日期:1985-12-01 00:00:00

  • From ER to Eph receptors: new roles for VAP fragments.

    abstract::Dominantly inherited mutations in an endoplasmic reticulum protein called VAPB have been found in a subset of patients with a rare familial form of amyotrophic lateral sclerosis (ALS). In this issue, Tsuda et al. (2008) identify a secreted form of VAPB that binds directly to Eph receptors inducing their activation and...


    pub_type: 评论,杂志文章


    authors: Ackerman SL,Cox GA

    更新日期:2008-06-13 00:00:00

  • Whence meiosis?

    abstract::Sexual reproduction predominates among eukaryotic organisms on our planet. While debate continues over why this should be so, burgeoning genomic and functional information now allows us to begin to think reasonably about some of the events that may have occurred to make sex possible in the first place. ...


    pub_type: 杂志文章,评审


    authors: Villeneuve AM,Hillers KJ

    更新日期:2001-09-21 00:00:00

  • Amyloid-like aggregates sequester numerous metastable proteins with essential cellular functions.

    abstract::Protein aggregation is linked with neurodegeneration and numerous other diseases by mechanisms that are not well understood. Here, we have analyzed the gain-of-function toxicity of artificial β sheet proteins that were designed to form amyloid-like fibrils. Using quantitative proteomics, we found that the toxicity of ...


    pub_type: 杂志文章


    authors: Olzscha H,Schermann SM,Woerner AC,Pinkert S,Hecht MH,Tartaglia GG,Vendruscolo M,Hayer-Hartl M,Hartl FU,Vabulas RM

    更新日期:2011-01-07 00:00:00

  • Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.

    abstract::Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromer...


    pub_type: 杂志文章


    authors: Hofmann RM,Pickart CM

    更新日期:1999-03-05 00:00:00

  • Pdd1p, a novel chromodomain-containing protein, links heterochromatin assembly and DNA elimination in Tetrahymena.

    abstract::During Tetrahymena conjugation, programmed DNA degradation occurs in two separate nuclei. Thousands of germline-specific deletion elements are removed from the genome of the developing somatic macronucleus, and the old parental macronucleus is degraded by an apoptotic mechanism. An abundant polypeptide, Pdd1p (formerl...


    pub_type: 杂志文章


    authors: Madireddi MT,Coyne RS,Smothers JF,Mickey KM,Yao MC,Allis CD

    更新日期:1996-10-04 00:00:00

  • Bone and Muscle Endocrine Functions: Unexpected Paradigms of Inter-organ Communication.

    abstract::Most physiological functions originate with the communication between organs. Mouse genetics has revived this holistic view of physiology through the identification of inter-organ communications that are unanticipated, functionally important, and would have been difficult to uncover otherwise. This Review highlights t...


    pub_type: 杂志文章,评审


    authors: Karsenty G,Olson EN

    更新日期:2016-03-10 00:00:00

  • Oncogenic Signaling Pathways in The Cancer Genome Atlas.

    abstract::Genetic alterations in signaling pathways that control cell-cycle progression, apoptosis, and cell growth are common hallmarks of cancer, but the extent, mechanisms, and co-occurrence of alterations in these pathways differ between individual tumors and tumor types. Using mutations, copy-number changes, mRNA expressio...


    pub_type: 杂志文章


    authors: Sanchez-Vega F,Mina M,Armenia J,Chatila WK,Luna A,La KC,Dimitriadoy S,Liu DL,Kantheti HS,Saghafinia S,Chakravarty D,Daian F,Gao Q,Bailey MH,Liang WW,Foltz SM,Shmulevich I,Ding L,Heins Z,Ochoa A,Gross B,Gao J,Z

    更新日期:2018-04-05 00:00:00

  • Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome.

    abstract::The p53 tumor suppressor gene is commonly altered in human tumors, predominantly through missense mutations that result in accumulation of mutant p53 protein. These mutations may confer dominant-negative or gain-of-function properties to p53. To ascertain the physiological effects of p53 point mutation, the structural...


    pub_type: 杂志文章


    authors: Olive KP,Tuveson DA,Ruhe ZC,Yin B,Willis NA,Bronson RT,Crowley D,Jacks T

    更新日期:2004-12-17 00:00:00

  • Probing the Diversity of T Cell Dysfunction in Cancer.

    abstract::T cell dysfunction in cancer comes in many forms, with two new varieties reported in this issue. Daley et al. find that T cells expressing γδ T cell receptors (TCR) promote pancreatic tumor growth by inhibiting activation of T cells with conventional TCRs. Singer et al. characterize dysfunctional tumor infiltrating ly...


    pub_type: 评论,杂志文章


    authors: Sowell RT,Kaech SM

    更新日期:2016-09-08 00:00:00

  • Cell interactions coordinate the development of the C. elegans egg-laying system.

    abstract::Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...


    pub_type: 杂志文章


    authors: Thomas JH,Stern MJ,Horvitz HR

    更新日期:1990-09-21 00:00:00

  • Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.

    abstract::Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...


    pub_type: 杂志文章


    authors: Méthot N,Basler K

    更新日期:1999-03-19 00:00:00

  • Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

    abstract::ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...


    pub_type: 杂志文章


    authors: Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

    更新日期:2012-10-26 00:00:00

  • BDNF instructs the kinase LKB1 to grow an axon.

    abstract::Determining which neurite of a differentiating neuron is to become the axon is a crucial step in neuronal morphogenesis. Two groups (Barnes et al., 2007; Shelly et al., 2007) now report that axon specification in vivo is mediated by extracellular signals acting through the serine/threonine kinase LKB1. ...


    pub_type: 评论,杂志文章


    authors: Winckler B

    更新日期:2007-05-04 00:00:00

  • Whole-genome analysis of dorsal-ventral patterning in the Drosophila embryo.

    abstract::The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tiss...


    pub_type: 杂志文章


    authors: Stathopoulos A,Van Drenth M,Erives A,Markstein M,Levine M

    更新日期:2002-11-27 00:00:00

  • Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.

    abstract::We have obtained the complete sequence of cloned full-length DNA (NS DNA) derived from influenza virus gene 8, which codes for two unique polypeptides, NS1 and NS2, and the sequence of the NS2 mRNA. Previously we showed that the mRNA for NS1 (approximately 860 nucleotides) is colinear with the viral RNA and maps from ...


    pub_type: 杂志文章


    authors: Lamb RA,Lai CJ

    更新日期:1980-09-01 00:00:00

  • A role for recombination junctions in the segregation of mitochondrial DNA in yeast.

    abstract::In S. cerevisiae, mitochondrial DNA (mtDNA) molecules, in spite of their high copy number, segregate as if there were a small number of heritable units. The rapid segregation of mitochondrial genomes can be analyzed using mtDNA deletion variants. These small, amplified genomes segregate preferentially from mixed zygot...


    pub_type: 杂志文章


    authors: Lockshon D,Zweifel SG,Freeman-Cook LL,Lorimer HE,Brewer BJ,Fangman WL

    更新日期:1995-06-16 00:00:00