An efficient method for fractionated whole rodent brain radiation.

Abstract:

OBJECTIVE:In order to test for mechanisms of whole brain radio therapy side effects and possible neuroprotective measures, a rodent model is desirable. In many models, a high single dose of 8-20 Gray (Gy) of whole brain irradiation is used. These experimental radiation protocols do not closely reflect the clinical situation, where the cumulative dosage is applied in smaller fractions. We describe an efficient method to perform repetitive, fractionated whole brain radio therapy to the rat brain. METHODS:Fifteen-week-old rats were irradiated with a dose of 5 or 10 Gy on four consecutive days, resulting in a cumulative dose in opposing fields of 20 Gy (n = 15) and 40 Gy (n = 17), respectively. Sham-irradiated rats (n = 14) received the same procedure but without application of cranial irradiation. Four collimators with a diameter of 3 cm each were used to place four rats and an ionization chamber simultaneously in the dose field for monitoring. RESULTS:Fourteen days after the procedure, irradiated animals showed decreased open-field activity (two-tailed t-test, sham versus 20 Gy, P<0.001; sham versus 40 Gy, P = 0.002), but no cognitive deficit as indicated by latencies in the Morris water maze test. Six weeks after the irradiation, no group showed alterations of histopathology such as vascular changes, demyelination, or white matter necrosis. DISCUSSION:The proposed model represents an efficient and safe method to perform fractioned high-dose irradiation of the rodent brain. Speculatively, it is possible to increase the cumulative dosage and dose per fraction used in this model to achieve a higher degree of radiation-induced toxicity.

journal_name

Neurol Res

journal_title

Neurological research

authors

Semmler A,Garbe S,Moskau S,Frisch C,Eter N,Schlegel U,Linnebank M

doi

10.1179/1743132812Y.0000000143

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

355-9

issue

4

eissn

0161-6412

issn

1743-1328

pii

ner2992

journal_volume

35

pub_type

杂志文章
  • Leptin's effect on accelerated fracture healing after traumatic brain injury.

    abstract:OBJECTIVE:To investigate mechanisms behind the faster rehabilitation of limb fractures when associated with traumatic brain injury (TBI). METHODS:New Zealand rabbits were divided into TBI group and sham-operation group for four studies as follows: (1) blood and cerebrospinal fluid (CSF) were drawn on days 1, 3, and 7 ...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/1743132813Y.0000000201

    authors: Yan H,Zhang HW,Fu P,Liu BL,Jin WZ,Duan SB,Xue J,Liu K,Sun ZM,Zeng XW

    更新日期:2013-06-01 00:00:00

  • Increased hippocampal CD38 and systemic inflammation after partial hepatectomy does not induce impairment of spatial cognition.

    abstract:OBJECTIVE:The role of inflammation in cognitive alterations in a post-operative setting is still not fully understood. Surgical interventions can cause systemic inflammations which eventually can induce neuroinflammation. However, the main causes of functional changes after surgery are still elusive. In this study, we ...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2016.1242452

    authors: Schnitzler S,Kopitz J,Plaschke K

    更新日期:2016-11-01 00:00:00

  • Comprehensive management of arteriovenous malformations.

    abstract::The treatment of arteriovenous malformations depends on the efforts of a multidisciplinary team whose ultimate goal is to achieve better results when compared to the natural history of the pathology. The role of adjuvant treatment modalities such as radiosurgery and endovascular embolization is discussed. Treatment st...

    journal_title:Neurological research

    pub_type: 杂志文章,评审

    doi:10.1080/01616412.1998.11740583

    authors: de Oliveira E,Tedeschi H,Raso J

    更新日期:1998-12-01 00:00:00

  • Experimental brain ischaemia: assessment of injury by magnetic resonance spectroscopy and histology.

    abstract::Phosphorus magnetic resonance spectroscopy (31P-MRS) was made to measure changes in brain high energy phosphate compounds, adenosine triphosphate (ATP) and phosphocreatine (PCr), inorganic phosphorus (Pi) and intracellular pH (pHi) during a prolonged period of incomplete brain ischaemia produced, in anaesthetized dogs...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1990.11739943

    authors: Anderson ML,Smith DS,Nioka S,Subramanian H,Garcia JH,Halsey JH,Chance B

    更新日期:1990-12-01 00:00:00

  • Exposure time and frequency of irradiation in brain tumours.

    abstract::The survival of a group of patients with malignant gliomas of the brain submitted to radical surgery and radiotherapy was studied. The average survival was nearly 4 times longer with X-ray treatment than with cobalt 60 irradiation when a comparison was made between 24 patients with glioblasto-astroblastoma (grade 3-4)...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1987.11739796

    authors: Christensen JC

    更新日期:1987-09-01 00:00:00

  • Administration of Vitamin D3 and E supplements reduces neuronal loss‏ and oxidative stress in a model of rats with Alzheimer's disease.

    abstract:INTRODUCTION:Alzheimer's disease (AD) is the most common neurodegenerative disease across the world. The major cause of AD is extensive oxidative stress and inflammation in central nervous system (CNS). Vitamin D3 and E are the most known vitamins that control oxidative stress and mitochondrial respiratory function. Th...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2020.1787624

    authors: Mehrabadi S,Sadr SS

    更新日期:2020-10-01 00:00:00

  • Diffusion tensor tractography to visualize axonal outgrowth and regeneration in a 4-cm reverse autograft sciatic nerve rabbit injury model.

    abstract:BACKGROUND:Diffusion tensor tractography (DTT) has recently been shown to accurately detect nerve injury and regeneration. This study assesses whether 7-tesla (7T) DTT imaging is a viable modality to observe axonal outgrowth in a 4 cm rabbit sciatic nerve injury model fixed by a reverse autograft (RA) surgical techniqu...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2018.1554284

    authors: Farinas AF,Pollins AC,Stephanides M,O'Neill D,Al-Kassis S,Esteve IVM,Colazo JM,Keller PR,Rankin T,Wormer BA,Kaoutzanis C,Dortch RD,Thayer WP

    更新日期:2019-03-01 00:00:00

  • Preventing the BDNF and NGF loss involved in the effects of cornel iridoid glycoside on attenuation of experimental autoimmune encephalomyelitis in mice.

    abstract::The present study was designed to investigate the beneficial effects of cornel iridoid glycoside (CIG), a main component extract from Cornus officinalis, on neurotrophin expression in mouse experimental autoimmune encephalomyelitis (EAE), a classical model of multiple sclerosis (MS). After EAE initiation, CIG was intr...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2016.1200766

    authors: Qu Z,Zheng N,Zhang Y,Zhang L,Liu J,Wang Q,Yin L

    更新日期:2016-09-01 00:00:00

  • Acetazolamide stimulation test in patients with unilateral internal carotid artery obstructions using transcranial Doppler and 99mTc-HM-PAO-Spect.

    abstract::Fifteen patients with symptoms of cerebral ischaemia and angiographically confirmed unilateral stenoses or occlusions of the extracranial internal carotid artery (ICA) and 20 controls were studied by a 2 MHz transcranial Doppler (TCD) at rest and after stimulation with 1 g acetazolamide i.v., a cerebral vasodilator. I...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1992.11740033

    authors: Rosenkranz K,Hierholzer J,Langer R,Hepp W,Palenker J,Felix R

    更新日期:1992-01-01 00:00:00

  • Gene expression in neurotrauma.

    abstract::It has been established that following injury to the central nervous system two types of damage take place, the initial insult and the secondary response to injury. This review will focus on the secondary molecular aspects of neurotrauma. These responses may be either deleterious or have protective effects upon the in...

    journal_title:Neurological research

    pub_type: 杂志文章,评审

    doi:10.1179/016164101101198343

    authors: Dutcher SA,Michael DB

    更新日期:2001-03-01 00:00:00

  • Positron emission tomographic evaluation of histological malignancy in gliomas using oxygen-15 and fluorine-18-fluorodeoxyglucose.

    abstract::HEADTOME III, a high resolution PET, has been employed using 15O and 18F labelled pharmaceuticals to evaluate histological malignancy of gliomas preoperatively. PET study was applied on eighteen preoperative gliomas including two recurrent cases. Haemocirculatory and metabolic indices of regional cerebral blood flow (...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1986.11739749

    authors: Mineura K,Yasuda T,Kowada M,Shishido F,Ogawa T,Uemura K

    更新日期:1986-09-01 00:00:00

  • Loss of essential membrane lipids and ascorbic acid from rat brain following cryogenic injury and protection by methylprednisolone.

    abstract::Previous work has shown that unsaturated fatty acid components of model membrane phospholipids in vitro, damaged via a free radical mechanism, are protected by the presence of cholesterol in these membranes. The participation of these membrane lipids in the pathogenesis of traumatic injury to brain was studied in vivo...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1981.11739608

    authors: Mitamura JA,Seligman ML,Solomon JJ,Flamm ES,Demopoulos HB,Ransohoff J

    更新日期:1981-01-01 00:00:00

  • Yoga-plus exercise mix promotes cognitive, affective, and physical functions in elderly people.

    abstract::Objectives: Increased attention is being paid to Asian medicine in balanced total health care. We investigated the effects of mixed exercise including yoga ('Yoga-plus') among elderly individuals. Methods: A total of 385 subjects (72 males and 313 females, 75.5 ± 8.7 years old) participated in a 12-month (M) exercise ...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2019.1672380

    authors: Hishikawa N,Takahashi Y,Fukui Y,Tokuchi R,Furusawa J,Takemoto M,Sato K,Yamashita T,Ohta Y,Abe K

    更新日期:2019-11-01 00:00:00

  • Transsphenoidal surgery for visual loss in patients with pituitary adenomas.

    abstract::This paper presents the results of transsphenoidal surgery in 41 consecutive patients with visual loss from pituitary adenomas. The most common pattern of loss was bitemporal hemianopia. Transsphenoidal decompression resulted in visual improvement in 78% of patients. If patients with optic atrophy and pre-operative co...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1990.11739907

    authors: el-Azouzi M,Black PM,Candia G,Zervas NT,Panagopoulos KP

    更新日期:1990-03-01 00:00:00

  • A comparative study on clinical characterizations between acute myelitis onset of neuromyelitis optica spectrum disease and idiopathic transverse myelitis.

    abstract:BACKGROUND/AIMS:Both of neuromyelitis optica spectrum disease (NMOSDs) and idiopathic transverse myelitis (ITM) could present as acute transverse myelitis. However, long-term immunological treatment and prognosis are different for high recurrence of NMOSDs. In this study, we summarized clinical differences between acut...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2020.1773628

    authors: Liu Z,Xie M,Lu Z,Zhang C,Chen H,Xu Y,Zhang M

    更新日期:2020-07-01 00:00:00

  • AST to ALT ratio and risk of hemorrhagic transformation in patients with acute ischemic stroke.

    abstract:BACKGROUND:Hemorrhagic transformation (HT) is a common complication of ischemic stroke. We performed this study to determine whether aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio (AAR) was related to HT, as well as compare the predictive values of AAR, ALT and AST for HT in patients with isch...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2020.1796403

    authors: Wang Y,Qiu K,Song Q,Cheng Y,Liu J,Liu M

    更新日期:2020-11-01 00:00:00

  • Cerebral and peripheral effects of laser needle-stimulation.

    abstract::This study comprises scientific-theoretic fundamental investigations of laserneedle technology, a new and painless method of acupuncture stimulation. Laserneedles are not inserted in the skin, but are merely placed on the surface of the acupuncture point. The study documents the significant changes in peripheral micro...

    journal_title:Neurological research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1179/016164103101202237

    authors: Litscher G

    更新日期:2003-10-01 00:00:00

  • Combined preoperative onyx embolization and protective internal carotid artery covered stent placement for treatment of glomus vagale tumor: review of literature and illustrative case.

    abstract:OBJECTIVE:Surgical resection of complex glomus vagale tumors can be complicated by extensive blood loss and might require surgical sacrifice of an encased internal carotid artery. METHODS:A young patient presented with mass effect from glomus valage tumor. Computerized tomography angiography showed an encased interna...

    journal_title:Neurological research

    pub_type: 杂志文章,评审

    doi:10.1179/1743132812Y.0000000036

    authors: Alaraj A,Pytynia K,Carlson AP,Krishna PH,Charbel FT,Amin-Hanjani S,Aletich V

    更新日期:2012-07-01 00:00:00

  • Cognitive impairment and whole brain diffusion in patients with carotid artery disease and ipsilateral transient ischemic attack.

    abstract:OBJECTIVES:Transient ischemic attack (TIA) with carotid artery disease (CAD) can increase the risk of cognitive decline. However, the neural mechanisms of the disorder remain unclear. The aim of this study was, using diffusion tensor imaging (DTI), to detect microstructural changes in patients with symptomatic CAD pres...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/1743132813Y.0000000255

    authors: Guo J,Wang S,Li R,Chen N,Zhou M,Chen H,Gong Q,He L

    更新日期:2014-01-01 00:00:00

  • The changes of systemic immune responses during the neuroprotection induced by remote ischemic postconditioning against focal cerebral ischemia in mice.

    abstract::Objective: Remote limb ischemic postconditioning (RIPostC) protects the brain from damage induced by transient focal ischemia/reperfusion. However, the underlying mechanism remains unclear. Methods: RIPostC induced by 10 min of occlusion and another 10 min releasing of blood flow for three cycles in the hind limbs was...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2018.1523037

    authors: Liu C,Yang J,Zhang C,Geng X,Zhao H

    更新日期:2019-01-01 00:00:00

  • Effects of popliteal illumination on cerebral near-infrared spectroscopy.

    abstract::The endogenous clock of humans can be influenced by light application to the popliteal region. Previously, it had been assumed that light must fall directly into the eye in order to influence the inner circadian clock. This study, published in 1998, has not been reproduced. We investigated the effects of laser light i...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/016164101101199388

    authors: Litscher G

    更新日期:2001-12-01 00:00:00

  • B-waves in healthy persons.

    abstract::The objective of this study was to determine, whether 0.5-2/min oscillations in intracranial pressure ('B-waves') are a physiological phenomenon. In a group of 5 patients 0.5-2/min oscillations of ventricular pressure simultaneous with oscillations in the middle cerebral artery (MCA) flow velocity, as assessed by tran...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1989.11739891

    authors: Mautner-Huppert D,Haberl RL,Dirnagl U,Villringer A,Schmiedek P,Einhäupl K

    更新日期:1989-12-01 00:00:00

  • Risk factors of stroke-associated pneumonia in Chinese patients.

    abstract:OBJECTIVE:To explore the risk factors for stroke-associated pneumonia (SAP). METHODS:A retrospective research study was carried out to investigate the clinical data of 1435 patients admitted to the neurological intensive care unit at our university hospital between 1 January 2000 and 31 December 2009. RESULTS:A multi...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/016164111X13007856084205

    authors: Sui R,Zhang L

    更新日期:2011-06-01 00:00:00

  • Thrombosis and recanalization of symptomatic nongiant saccular aneurysm.

    abstract::Complete thrombosis followed by recanalization of giant aneurysms is an infrequent process that has been exceptionally mentioned in relation to nongiant saccular aneurysms. A 25-year-old male presented with a right temporal hematoma and associated subarachnoid hemorrhage. Cerebral angiography revealed a small blister-...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/016164103771953961

    authors: Cohen JE,Rajz G,Umansky F,Spektor S

    更新日期:2003-12-01 00:00:00

  • Spontaneous disappearance and reappearance of a ruptured cerebral aneurysm: one case found in a group of 33 consecutive patients with subarachnoid hemorrhage who underwent repeat angiography.

    abstract::The spontaneous disappearance and reappearance of a ruptured cerebral aneurysm is generally assumed to be a rare phenomenon although the actual incidence is unknown. Among 39 consecutive cases of acute subarachnoid hemorrhage (SAH), 33 were studied by three-dimensional computed tomographic angiography (CTA) within 6 h...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.2000.11740723

    authors: Nakajima Y,Yoshimine T,Mori H,Nakamuta K,Fujimura I,Sakashita K,Kohmura E,Hayakawa T,Yokota J

    更新日期:2000-09-01 00:00:00

  • The temporal profile and morphologic features of neuronal death in human stroke resemble those observed in experimental forebrain ischemia: the potential role of apoptosis.

    abstract::Although neuronal death has been studied in experimental models of ischemia, the precise mechanisms regulating cell death remain unclear. Furthermore, the timing and pattern of neuronal death in human stroke has not been extensively studied. To further our understanding of ischemia-induced neuronal death, we examined ...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1998.11740520

    authors: Guglielmo MA,Chan PT,Cortez S,Stopa EG,McMillan P,Johanson CE,Epstein M,Doberstein CE

    更新日期:1998-06-01 00:00:00

  • Tissue plasminogen activator and plasminogen activator inhibitor in patients with acute ischemic stroke: relation to stroke etiology.

    abstract::Recent studies suggest that high plasma levels of tissue-type plasminogen activator (tPA) and its inhibitor (plasminogen activator inhibitor-1, PAI-1) are markers of an increased risk of atherothrombotic ischemic events such as stroke and myocardial infarction. In this prospective study, we measured tPA antigen, PAI-1...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1999.11741005

    authors: Zunker P,Schick A,Padró T,Kienast J,Phillips A,Ringelstein EB

    更新日期:1999-12-01 00:00:00

  • Anti-GQ1b IgG antibody activities related to the severity of Miller Fisher syndrome.

    abstract::The correlation between the activities of anti-GQ1b IgG antibodies in sera of patients with Miller Fisher syndrome and their clinical manifestations was investigated. The clinical severity was analyzed for 16 patients by scoring their symptoms during the peak of the clinical course and 4 weeks after onset. Serum sampl...

    journal_title:Neurological research

    pub_type: 临床试验,杂志文章

    doi:10.1080/01616412.1998.11740573

    authors: Mizoguchi K

    更新日期:1998-10-01 00:00:00

  • Silent cerebral ischemic lesions after carotid artery stenting with distal cerebral protection.

    abstract:OBJECTIVE:To evaluate by MR diffusion weighted image (DWI-MR) the presence of new ischemic cerebral lesions after carotid artery stenting (CAS) and distal cerebral protection, in patients with internal carotid artery (ICA) stenosis >70. METHODS:Sixty-seven CAS were performed under cerebral protection with a distal fil...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1179/016164105X35431

    authors: González A,Piñero P,Martínez E,González-Marcos JR,Rafel E,Mayol A,Gil-Peralta A

    更新日期:2005-01-01 00:00:00

  • Evaluation of the regional cerebral vasodilatory capacity before carotid endarterectomy by the acetazolamide test.

    abstract::To estimate the regional perfusion pressure and possibly the stump pressure during carotid endarterectomy, cerebral blood flow (CBF) measurements including a vasodilatory test were performed preoperatively. CBF was measured by 133Xe inhalation and emission tomography. An intravenous dose of 1g acetazolamide (Diamox) w...

    journal_title:Neurological research

    pub_type: 杂志文章

    doi:10.1080/01616412.1987.11739765

    authors: Vorstrup S,Boysen G,Brun B,Engell HC

    更新日期:1987-03-01 00:00:00