Abstract：
:The biological function of selenium (Se) is mainly elicited through Se-containing proteins. Selenoprotein W (SelW), one member of the selenoprotein family, is essential for the normal function of the skeletal muscle system. To investigate the possible relationship of Se in the process of differentiation in chicken myoblasts and the expression of SelW, the cultured chicken embryonic myoblasts were incubated with sodium selenite at different concentrations for 72 h, and then the mRNA levels of SelW and myogenic regulatory factors (MRFs) in myoblasts were determined at 12, 24, 48, and 72 h, respectively. Furthermore, the correlation between SelW mRNA expression and MRF mRNA expression was assessed. The results showed that the sodium selenite medium enhanced the mRNA expression of SelW, Myf-5, MRF4, and myogenin in chicken myoblasts. The mRNA expression levels of MRFs were significantly correlated with those of SelW at 24, 48, and 72 h. These data demonstrate that Se is involved in the differentiation of chicken embryonic myoblasts, and SelW showed correlation with MRFs.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Wu Q,Yao HD,Zhang ZW,Zhang B,Meng FY,Xu SW,Wang XLdoi
10.1007/s12011-012-9520-8subject
Has Abstractpub_date
2012-12-01 00:00:00pages
166-72issue
1-3eissn
0163-4984issn
1559-0720journal_volume
150pub_type
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