当前位置： SCI文献检索 > RHEUMATOLOGY INTERNATIONAL期刊下所有文献 > Impact of sphingosine kinase 2 deficiency on the development of TNF-alpha-induced inflammatory arthritis.

Impact of sphingosine kinase 2 deficiency on the development of TNF-alpha-induced inflammatory arthritis.

Abstract：

:Sphingolipids are components of the plasma membrane whose metabolic manipulation is of interest as a potential therapeutic approach in a number of diseases. Sphingosine kinase 1 (SphK1), the major kinase that phosphorylates sphingosine to sphingosine-1-phosphate (S1P), was previously shown by our group and others to modulate inflammation in murine models of inflammatory arthritis, inflammatory bowel disease and asthma. Sphingosine kinase 2's (SphK2) impact on inflammation is less well known, as variable results were reported depending on the disease model. A specific SphK2 inhibitor inhibited inflammatory arthritis in one model, while siRNA knockdown of SphK2 worsened arthritis in another. We previously demonstrated that SphK1 deficient mice are protected against development of hTNF-α-induced arthritis. To investigate the role of SphK2 in TNF-α-induced arthritis, we developed SphK2 deficient hTNF-α overexpressing mice and separately treated hTNF-α mice with ABC294640, a SphK2-specific inhibitor. Our data show that genetic inhibition of SphK2 did not significantly impact the severity or progression of inflammatory arthritis, while pharmacologic inhibition of SphK2 led to significantly more severe arthritis. Compared to vehicle-treated mice, ABC294640 treated mice also had less S1P in whole blood and inflamed joint tissue, although the differences were not significant. ABC294640 treatment did not affect SphK1 activity in the inflamed joint while little SphK2 activity was detected in the joint. We conclude that the differences in the inflammatory phenotype in genetic inhibition versus pharmacologic inhibition of SphK2 can be attributed to the amount of ABC294640 used in the experiments versus the impact of acute inhibition of SphK2 with ABC294640 versus genetically induced life-long SphK2 deficiency. Thus, inhibition of SphK2 appears to be proinflammatory in contrast to the clear anti-inflammatory effects of blocking SphK1. Therapies directed at this sphingosine kinase pathways will need to be specific in their targeting of sphingosine kinases.

journal_name

Rheumatol Int

journal_title

Rheumatology international

authors

Baker DA,Eudaly J,Smith CD,Obeid LM,Gilkeson GS

doi

10.1007/s00296-012-2493-2

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

2677-81

issue

eissn

0172-8172

issn

1437-160X

journal_volume

pub_type

杂志文章

在线工具

Impact of sphingosine kinase 2 deficiency on the development of TNF-alpha-induced inflammatory arthritis.