Abstract:
CONTEXT:Cucurbitacins are a group of triterpenoids that have a cucurbitane skeleton with a wide range of biological activities. OBJECTIVES:This study evaluated the anticancer properties of one cucurbitacin isolated from Cayaponia racemosa Cong. (Cucurbitaceae), 2β,3β,16α,20(R),25-pentahydroxy-22-oxocucurbita-5-en (1), with in vitro and in vivo models. MATERIALS AND METHODS:In vitro cytotoxic activity was determined with human leukemia (HL60) and normal blood cells (PBMC). Sarcoma 180 was used as in vivo model. RESULTS:The cucurbitacin (1) reduced the number of viable cells; however, there was no changed in the number of non-viable cells at 5 µg/mL. Selectivity towards cancer cells was suggested by the absence of activity on normal proliferating lymphocytes at the concentrations tested (IC₅₀ >25 µg/ml). Morphological analysis of compound 1-treated cells showed typical apoptotic features, such as intense deposition of granules in the cytoplasm (eosinophilia), DNA fragmentation and irregularities in the plasma membrane. In addition, the cells treated with compound 1 presented intense vacuolization and disruption of the plasma membrane. Acridine orange/Ethidium bromide staining confirmed these findings, revealing an increased number of apoptotic cells. In the Sarcoma 180 tumor model, compound 1 showed 52 and 62% of antitumor activity, either alone (25 mg/kg/day) or in association with the chemotherapeutic agent 5-FU (10 + 10 mg/kg/day), respectively. Moreover, either alone or associated with 5-FU, treatment with compound 1 caused an increase in spleen weight and morphological alterations related to immunostimulatory properties. CONCLUSION:These data indicate that these naturally occurring compounds have anticancer potential.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Militão GC,Dantas IN,Ferreira PM,Alves AP,Chaves DC,Monte FJ,Pessoa C,Odorico de Moraes M,Costa-Lotufo LVdoi
10.3109/13880209.2012.684691subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1479-87issue
12eissn
1388-0209issn
1744-5116journal_volume
50pub_type
杂志文章abstract:CONTEXT:Viola tianshanica Maxim. (Violaceae) is a perennial herb distributed in Central Asia, especially in the Xinjiang Uygur Autonomous Region (XUAR) of China. Preliminary study showed that the ethanol extract of the herb exhibited the anti-complement activity against the classical pathway, but the active components ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1055635
更新日期:2016-07-01 00:00:00
abstract:CONTEXT:Alpinia oxyphylla Miquel (Zingiberaceae) is a traditional Chinese herbal medicine widely used for the treatment of intestinal disorders, urosis and diuresis. However, information about antioxidant and cytotoxic properties of its fruits remains to be elucidated. OBJECTIVE:The ethanol crude extract (CE) and its ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.794844
更新日期:2013-11-01 00:00:00
abstract:CONTEXT:Lycopene is a phytonutrient under considerable investigation for its antioxidant benefits in treating diseases like cancer, cardiovascular diseases, osteoporosis and diabetes. OBJECTIVE:This study explores the effect of lycopene against oxidative damage during experimental hepatitis, induced by D-galactosamine...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.803579
更新日期:2013-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.924149
更新日期:2015-04-01 00:00:00
abstract:CONTEXT:Buchenavia tetraphylla (Aubl.) RA Howard (Combretaceae: Combretoideae) is an ethnomedicinal plant with reported antifungal action. OBJECTIVE:This study evaluates the antimicrobial activity of B. tetraphylla leaf extracts against clinical isolates of Candida albicans. The morphological alterations, combinatory ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.3109/13880209.2011.558515
更新日期:2011-09-01 00:00:00
abstract:CONTEXT:Despite phytochemical studies of Agrimonia pilosa Ledeb. (Rosaceae), the antidiabetic effects of this plant are unknown. OBJECTIVE:This study characterizes the isolated compounds from the aerial parts of A. pilosa and evaluates their PTP1B and α-glucosidase inhibitory properties. MATERIALS AND METHODS:Ethanol...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1048372
更新日期:2016-01-01 00:00:00
abstract::The activity of an (8-hydroxymethylen)-trieicosanyl acetate compound obtained from chloroform extracts of Senna villosa (Mill.) H.S. Irwin & Barneby (Leguminosae) against Trypanosoma cruzi was evaluated in vivo. Oral doses of 2.1, 8.4, and 33.6 microg/g were tested for 28 days in BALB/c mice infected with T. cruzi. Re...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903241853
更新日期:2010-06-01 00:00:00
abstract::Chemical constituents as well as cytotoxic and insecticidal activity of the crude methanol extract from the leaves of Phyllanthus reticulatus Poir. (Euphorbiaceae) were investigated. (5R*,6R*)-4,6-Dimethoxycarbonyl-5-[2',3',4'-trihydroxy-6'-(methoxycarbonyl) phenyl]-5,6-dihydro-2H-pyran-2-one (1) along with 3,4,3'-tri...
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pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. The use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity. OBJECTIVE:A study was conducted to i...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880201003713598
更新日期:2010-11-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2017.1418391
更新日期:2018-12-01 00:00:00
abstract::Escin, a group of chemically related triterpenic glycosides, is widely used in commercial preparations for the treatment of venous insufficiency. Since the zygotic embryo cotyledons accumulate the highest amount of escin, it is currently extracted from the seeds of horse chestnut, Aesculus hippocastanum L. (Hippocasta...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.880487
更新日期:2014-09-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2016.1172245
更新日期:2016-11-01 00:00:00
abstract:CONTEXT:Balanites aegyptiaca (L.) Delile (Zygophyllaceae) is a tropical tree that has many folk uses in various countries. The bark extract is used for the control of the fresh water snails that act as intermediary host of Schistosoma. OBJECTIVE:Study the molluscicidal activity and chemical constituents of seed oil an...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.674950
更新日期:2012-10-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.900809
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.716851
更新日期:2013-02-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.784920
更新日期:2013-10-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.818041
更新日期:2014-02-01 00:00:00
abstract::Context Syzygium densiflorum Wall. ex Wight & Arn (Myrtaceae) has been traditionally used by local tribes of the Nilgiris, Tamil Nadu, India, for the treatment of diabetes, however, no definitive experimental studies are available. Objective This study investigates the antidiabetic, antihyperlipidaemic and antioxidant...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1125932
更新日期:2016-09-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2016.1181659
更新日期:2016-11-01 00:00:00
abstract:CONTEXT:Dexamethasone (DXM) has an anti-immunoinflammatory effect, and is often used in acute kidney injury (AKI). However, the effects of DXM on albumin (ALB) have not been fully studied. OBJECTIVE:To investigate the effects of DXM on ALB production and renal function. MATERIALS AND METHODS:Male Wistar rats were div...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2020.1855214
更新日期:2020-12-01 00:00:00
abstract:CONTEXT:The genus Premna (Lamiaceae), distributed throughout tropical and subtropical Asia, Africa, Australia and the Pacific Islands, is used in folk medicine primarily to treat inflammation, immune-related diseases, stomach disorders, wound healing, and skin diseases. OBJECTIVES:This review exhaustively gathers avai...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
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更新日期:2017-12-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.776612
更新日期:2013-08-01 00:00:00
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journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.791324
更新日期:2013-11-01 00:00:00
abstract:CONTEXT:The fruit of the Prunus mume Sieb. et Zucc (Rosaceae) is used as a health food or medicinal material in traditional herb medicine for a long time in Eastern Asian countries. OBJECTIVE:Our present study investigated the hypouricemic effect of the methanol extract from P. mume fruit (MPMF) in mice with potassium...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.683115
更新日期:2012-11-01 00:00:00