Abstract:
:Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) are used as first-line agents for treating nonsquamous cell lung cancer with EGFR mutation, there are many patients who have to receive these drugs following platinum-based chemotherapy. This study was designed to define whether exposure to cisplatin could affect the sensitivity to EGFR TKIs because conflicting results have been presented. We established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827). The EGFR-related signals were examined by Western blotting. MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were measured in animal models. The IC50 value and apoptotic fractions after exposure to EGFR TKIs, such as gefitinib, erlotinib, and BIBW 2992, were almost the same in the cisplatin-resistant sublines compared to that of the parent cells. Although the baseline PTEN expression was reduced in the resistant cells, as was indicated in a previous study, the EGFR-related signals similarly responded to the EGFR TKIs. Furthermore, the reduced tumor size and SUV of the 18FDG-PET of the implanted tumor in nude mice according to erlotinib treatment were not different between the resistant sublines and the parent cells. In conclusion, the acquired resistance to cisplatin did not affect the sensitivity to EGFR TKIs in the EGFR mutant lung cancer cells, and this should abrogate any concerns about the use of EGFR TKIs following platinum-based chemotherapy.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Rho JK,Choi YJ,Choi YR,Kim SY,Choi SJ,Choi CM,Na II,Lee JCdoi
10.3727/096504012x13285365944337subject
Has Abstractpub_date
2011-01-01 00:00:00pages
471-8issue
10-11eissn
0965-0407issn
1555-3906journal_volume
19pub_type
杂志文章abstract::To ascertain whether insulin-like growth factors (IGF-1 and IGF-2) affect the estrogen- and insulin-responsive R3230AC mammary carcinoma, studies of IGF-1 and IGF-2 receptors were conducted on primary-culture tumor cells and on membranes purified from whole tumors. By saturation binding analysis, cells in culture disp...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Since recombinant human granulocyte colony-stimulating factor (rhG-CSF) recruits hematopoietic progenitor cells into the cell cycle, the possibility remains that simultaneous injection of rhG-CSF and chemotherapeutic agents may aggravate the chemotherapy-induced neutropenia by sensitizing normal progenitor cells to th...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14841698396829
更新日期:2017-08-07 00:00:00
abstract::Ahead of Print article withdrawn by publisher. ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14747357310629
更新日期:2016-09-30 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is commonly overexpressed in non-small cell lung cancer (NSCLC) and its tyrosine kinase phosphorylation is thought to be an ideal target in the treatment of patients with NSCLC. In the present study, we examined surgically obtained specimens from a series of 36 NSCLC patients fo...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504003108748348
更新日期:2003-01-01 00:00:00
abstract::Ahead of Print article withdrawn by publisher. ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14974821032421
更新日期:2017-06-15 00:00:00
abstract::The pre-mRNA splicing regulator serine-arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this stud...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14743324568129
更新日期:2017-03-13 00:00:00
abstract::Phytochemicals present in tea, particularly polyphenols, have anticancer properties against several cancer types. However, studies elucidating the role and the mechanism(s) of action of tea polyphenols in cervical cancer are sparse. In this study, we investigated the mechanism of antiproliferative and apoptotic action...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504011x13021877989711
更新日期:2011-01-01 00:00:00
abstract::Osteosarcoma is the most common primary bone malignancy manifested predominantly in children and young adults. Studies indicate that miR-107 is involved in the pathogenesis of osteosarcoma and that tropomyosin 1 (TPM1) acts as a tumor suppressor in many types of cancer. In this study, we analyzed the effect of miR-107...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14882829077237
更新日期:2017-09-21 00:00:00
abstract::The aim of this study was to analyze the efficacy according to EGFR status and predictors of TKIs in Chinese advanced lung adenocarcinoma patients in a single institute. We retrospectively enrolled 253 patients with advanced or recurrent adenocarcinoma and history of EGFR-TKI treatment attended at Beijing Chest Hospit...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504014X13907540404833
更新日期:2014-01-01 00:00:00
abstract::Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP/PBXIP1) is a nucleo-cytoplasmic shuttling protein, and its expression is associated with cancer aggressiveness. However, the role of HPIP in ovarian cancer is still unclear. Here, we aimed to clarify the role of HPIP in epithelial-m...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14575597858654
更新日期:2016-01-01 00:00:00
abstract::We applied a differential display method to screen mRNAs isolated from a newly established cell line that carried a wild-type p53 transgene under control of the lactose operon. To investigate the p53 signaling pathway, we looked for genes whose expression was significantly induced or suppressed by induction of wild-ty...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Expression of sphingosine kinase 1 (SPHK1) plays a role in colorectal cancer progression. This study aimed to demonstrate the mechanism of human colorectal cancer cell metastatic phenotype through SPHK1 knockdown. Human colorectal cancer HT-29 cells were stimulated by phorbol 12-myristate 13-acetate (PMA) with or with...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504012x13342463747450
更新日期:2011-01-01 00:00:00
abstract::HeLa cells were transfected with full-length multidrug resistance protein (MRP) cDNA and with MRP cDNAs that had been mutated at certain nucleotide binding domains. Stable transfectants were isolated and those producing equivalent amounts of P190 were tested in cytotoxicity assays using a variety of chemotherapeutic a...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNA...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14813859905646
更新日期:2017-07-05 00:00:00
abstract::Previous studies demonstrated that an antisense phosphorothioate oligodeoxynucleotide, ISIS 3466, to the human nucleolar p120 protein, markedly inhibited the growth of human tumor cell lines in vitro and inhibited the growth of the human LOX tumor in vivo in an i.p./i.p. model, in the presence of DOTMA (Perlaky et al....
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The anticancer drug Adriamycin is widely used in cancer chemotherapy and is classified as a topoisomerase II inhibitor. However, in the presence of formaldehyde, Adriamycin also forms high levels of DNA adducts. In this study, a new series of butyric acid and formaldehyde-releasing drugs related to AN9 (pivaloyloxymet...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504005776382305
更新日期:2005-01-01 00:00:00
abstract::Sixteen desmuramyl analogs of muramyl dipeptide (MDP) were tested for their abilities to stimulate cytotoxic factor secretion by mouse peritoneal macrophages. Among them, the pseudohexapeptide Boc-Gly psi [CH2O]-D-Ala-Ala-D-Glu[Lys(H-Gly)NHEt]-NH2 appeared to be four times more effective than MDP. From this study, the...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Human papillomavirus type 16 (HPV16) is a major causative factor in the development of uterine cervical carcinomas. We investigated the role of E6/E7 in tumor formation. Skin-specific E6/E7 transgenic mice showed approximately twice as many tumors compared with nontransgenic mice in dimethylbenz[a]anthracene (DMBA)-in...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/000000006783980964
更新日期:2007-01-01 00:00:00
abstract::This study aimed to explore the effects of lncRNA BCAR4 on the viability and aggressiveness of non-small cell lung cancer (NSCLC) cells. qRT-PCR was used to determine the expression of BCAR4 and GLI2 downstream genes in NSCLC tissues and cell lines. Chromatin isolation by RNA purification (CHIRP) and Western blot were...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15220594629967
更新日期:2019-02-21 00:00:00
abstract::Constitutive canonical Wnt signaling, resulting from mutations in the adenomatous polyposis coli (APC), beta-catenin, or axin genes, has been implicated in the initiation of most human colorectal cancers (CRCs). Some of the proposed approaches for CRC prevention and treatment involve the downregulation of canonical Wn...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:10.3727/096504008784046108
更新日期:2008-01-01 00:00:00
abstract::Topotecan, a topoisomerase I poison and water-soluble derivative of camptothecin, has shown promise in treating solid tumors; however, the drug is unstable under physiological conditions and converts to an inactive form within 30 minutes. Encapsulating topotecan in liposomes (LIP-TPT) minimizes inactivation. The effic...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::3'-Azido-3'-deoxythymidine (AZT) competitively inhibited the transport of thymidine (Km = 0.23 mM) into human erythrocytes with a Ki of 1.0 mM at 37 degrees C. The principal human metabolite of AZT in plasma, the 5'-glucuronide (GAZT), was a weak inhibitor of the nucleoside transporter (< 20% inhibition of the influx ...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Heme oxygenase-1 (HO-1) plays an important role in the progression of several malignancies including breast cancer. However, its role in breast cancer metastasis is still ambiguous. In this study, we observed the effect of HO-1 on mouse mammary carcinoma metastasis using the in vivo tumor metastasis model. Our results...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15415906335771
更新日期:2019-06-21 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC ce...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14685034103950
更新日期:2016-10-27 00:00:00
abstract::This commentary proposes the working hypothesis that transforming growth factor-beta (TGF-beta) can play two different and opposite roles with respect to the process of malignant progression. During the earliest stages of carcinogenesis, TGF-beta can act as potent tumor suppressor and may mediate the actions of chemop...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract::The poly(ADP-ribose) polymerase (PARP) enzymes play a key role in the regulation of cellular processes (e.g., DNA damage repair, genomic stability). It has been shown that PARP inhibitors (PARPIs) are selectively cytotoxic against cells having dysfunctions in genes involved in DNA repair mechanisms (synthetic lethalit...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14926854178616
更新日期:2017-11-02 00:00:00
abstract::Human glioblastoma multiforme (GBM) accounts for the majority of human brain gliomas. Several TMEM proteins, such as TMEM 45A, TMEM 97, and TMEM 140, are implicated in human brain gliomas. However, the roles of TMEM168 in human GBM remain poorly understood. Herein we found that mRNA levels of TMEM168 were overexpresse...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15478559215014
更新日期:2019-07-12 00:00:00
abstract::Gastric cancer (GC) is the most common epithelial malignancy worldwide. Basic transcription factor 3 (BTF3) plays a crucial role in the regulation of various biological processes. We designed experiments to investigate the molecular mechanism underlying the role of BTF3 in GC cell proliferation and metastasis. We conf...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14886494526344
更新日期:2017-11-02 00:00:00
abstract::Aclacinomycin (ACR) is an anthracycline anticancer drug that shows marked effects in Adriamycin (ADM)-resistant tumors. ADM, however, is not effective against ACR-resistant tumor cells. When tumor cells acquire resistance to ACR, though the resistance is not easily acquired, they show strong cross-resistance to ADM. T...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00