Abstract:
:Topotecan, a topoisomerase I poison and water-soluble derivative of camptothecin, has shown promise in treating solid tumors; however, the drug is unstable under physiological conditions and converts to an inactive form within 30 minutes. Encapsulating topotecan in liposomes (LIP-TPT) minimizes inactivation. The efficacy of LIP-TPT was examined with a novel in vivo bioassay called ICE for In vivo Complexes of Enzyme. This bioassay uses antibodies to probe DNA for the presence of topoisomerase I covalent complexes and thereby allows direct quantification of topoisomerase I driven DNA adducts in living cells. We report that LIP-TPT was three- to fourfold more effective than free TPT in stabilizing covalent topoisomerase I-DNA intermediates inside tumor cells. These findings reveal that liposomal wrapping permitted effective delivery of camptothecin derivatives to active enzyme in the nucleus of the cell.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Subramanian D,Muller MTsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
461-9issue
9eissn
0965-0407issn
1555-3906journal_volume
7pub_type
杂志文章abstract::Treatment of female SKH-1 hairless mice with ultraviolet B light twice a week for 20 weeks resulted in a population of tumor-free mice with a high risk of developing skin tumors during the next several months in the absence of additional UVB treatment (high-risk mice). Topical applications of nondenatured soymilk but ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/0965040041292350
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journal_title:Oncology research
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doi:10.3727/000000001108747381
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journal_title:Oncology research
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doi:10.3727/096504017X14926854178616
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journal_title:Oncology research
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doi:10.3727/096504010x12767359113848
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journal_title:Oncology research
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journal_title:Oncology research
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doi:10.3727/096504020X15954139263808
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journal_title:Oncology research
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doi:10.3727/096504016X14756640150695
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504008786991611
更新日期:2008-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14965111926391
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journal_title:Oncology research
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Oncology research
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journal_title:Oncology research
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15140544654946
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journal_title:Oncology research
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更新日期:2014-01-01 00:00:00
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doi:10.3727/096504018X15336368805108
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Oncology research
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doi:10.3727/096504014X13907540404833
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journal_title:Oncology research
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更新日期:1996-01-01 00:00:00
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doi:10.3727/096504012x13522227232156
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journal_title:Oncology research
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journal_title:Oncology research
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