Abstract:
:The clinical study of compounds that modulate multidrug resistance has been hindered by both the toxicities of these agents and the inability to monitor their effectiveness at the level of the tumor cell. Previously, toremifene has been shown to be well tolerated clinically and to sensitize multidrug resistant cells to the effects of cytotoxic chemotherapeutic agents. The chemosensitizing properties of toremifene in estrogen receptor negative, multidrug resistant MDA-MB-A1 human breast cancer cells were studied using flow cytometric analysis and growth inhibition assays. Cell cycle kinetics of MDA-MB-A1 cells were not significantly affected by treatment with either toremifene, N-desmethyltoremifene, Toremifene IV or vinblastine alone, as the majority of cells remained in G0/G1. However, preincubation with toremifene or one of its metabolites for 72 hours followed by treatment for one hour with vinblastine caused a marked shift of cells to G2/M, as cells appeared to be blocked in that phase of the cell cycle. This result was nearly identical to the effect of vinblastine alone on vinblastine-sensitive MDA-MB-231 breast cancer cells and can be interpreted as a "resensitization" by toremifene of MDA-MB-A1 cells to vinblastine. This chemosensitizing effect of toremifene was accompanied by an enhanced inhibition of cell growth by vinblastine. The chemosensitizing effects of toremifene or one of its metabolites in combination with cytotoxic chemotherapy can be effectively monitored by flow cytometry, an easily accessible technique.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Baker WJ,Maenpaa JU,Wurz GT,Koester SK,Seymour RC,Emshoff VD,Wiebe VJ,DeGregorio MWsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
207-12issue
6-7eissn
0965-0407issn
1555-3906journal_volume
5pub_type
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15112639918174
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journal_title:Oncology research
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doi:10.3727/096504012x13342463747450
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journal_title:Oncology research
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doi:10.3727/096504018X15213142076069
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504008786991611
更新日期:2008-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
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journal_title:Oncology research
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doi:10.3727/096504013x13639794277680
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Angiogenesis, the formation of new vessels, is essential for tumor growth and metastasis. Mutations of p53 tumor suppressor gene are frequent and play an important role in colorectal oncogenesis. A role of p53 as an angiogenesis inhibitor has also been proposed. We evaluated angiogenesis and p53 expression in 16 hyper...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504001108747693
更新日期:2000-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14965111926391
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504007783438349
更新日期:2007-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504010x12704916124864
更新日期:2010-01-01 00:00:00
abstract::We applied a differential display method to screen mRNAs isolated from a newly established cell line that carried a wild-type p53 transgene under control of the lactose operon. To investigate the p53 signaling pathway, we looked for genes whose expression was significantly induced or suppressed by induction of wild-ty...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/000000003108747983
更新日期:2003-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14721731148893
更新日期:2017-04-14 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14873430389189
更新日期:2017-09-21 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504010x12671222663593
更新日期:2010-01-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is commonly overexpressed in non-small cell lung cancer (NSCLC) and its tyrosine kinase phosphorylation is thought to be an ideal target in the treatment of patients with NSCLC. In the present study, we examined surgically obtained specimens from a series of 36 NSCLC patients fo...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504003108748348
更新日期:2003-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:
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journal_title:Oncology research
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journal_title:Oncology research
pub_type: 杂志文章
doi:
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journal_title:Oncology research
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/0965040041292350
更新日期:2004-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14841698396829
更新日期:2017-08-07 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/000000003108748595
更新日期:2003-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Oncology research
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journal_title:Oncology research
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doi:10.3727/096504017X14954936991990
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journal_title:Oncology research
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00