Abstract：
:Recently, there has been significant effort in developing techniques designed to detect disseminated tumor cells in the peripheral blood (PB). These techniques include immunocytochemical staining of cytocentrifuge slides, flow cytometry, and RT-PCR. Several authors reported various results concerning the sensitivity of the detection limit when applying these methods. The aim of this study was to assess the value of two methods in the detection of ovarian carcinoma cells in the PB. For tumor cell detection we compared RT-PCR to immunomagnetic enrichment followed by flow cytometric analysis. In a model system, single cell suspensions of ovarian cancer cell lines were mixed with full blood samples from healthy donors in order to determine the sensitivity limit of the two methods and to analyze the reproducibility of each. In a multiparameter flow cytometric analysis, tumor cells were defined as cytokeratin 7/8 positive and CD45 negative. RNA was screened for MUC1 mRNA by RT-PCR. MUC1 mRNA expression turned out not to be a specific marker of disseminated ovarian cancer cells, because a weak expression was also found in samples of healthy persons. Using immunomagnetic enrichment followed by flow cytometry, one carcinoma cell per 1 x 10(6) leukocytes was detectable. However, a minimum of 10 ml blood had to be analyzed in order to clearly distinguish real positive tumor cells from false-positive signals.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Stimpfl M,Schmid BC,Obermair A,Tong D,Schiebel I,Gitsch G,Leodolter S,Zeillinger Rsubject
Has Abstractpub_date
1999-01-01 00:00:00pages
367-73issue
8eissn
0965-0407issn
1555-3906journal_volume
11pub_type
杂志文章abstract：:Previous studies demonstrated that an antisense phosphorothioate oligodeoxynucleotide, ISIS 3466, to the human nucleolar p120 protein, markedly inhibited the growth of human tumor cell lines in vitro and inhibited the growth of the human LOX tumor in vivo in an i.p./i.p. model, in the presence of DOTMA (Perlaky et al....
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Ahead of Print article withdrawn by publisher. ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14747357310629
更新日期：2016-09-30 00:00:00
abstract：:Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) (p16INK4A/MTS1) tumor suppressor gene has been found in a variety of human tumor types. To investigate the involvement of CDKI abnormality in mesenchymal chondrosarcoma, alterations of CDKIs were examined in human mesenchymal chondrosarcoma tiss...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000001108747381
更新日期：2000-01-01 00:00:00
abstract：:All-trans-Retinoic acid (RA) induces differentiation and inhibits growth of many tumor types. Whereas the RA nuclear receptors mediate genomic effects of RA, there also are many nongenomic effects that do not have defined mechanisms. Some nongenomic effects of RA may involve retinoylation (RA acylation), a posttransla...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Because of lack of effective treatment options for hormone-refractory prostate cancer at the present time, the need for developing novel therapeutic strategies and targets to treat and prevent the progression of hormone-sensitive prostate cancer to the hormone-refractory stage is paramount. Our previous in vitro studi...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008786991611
更新日期：2008-01-01 00:00:00
abstract：:To elucidate the proteins related to the development and progress of esophageal squamous cell carcinoma (ESCC), we performed gene expression profile analysis of 14 ESCC tissues. We identified 182 genes that were commonly upregulated (p < 10(-5)) and 54 genes that were downregulated in ESCC tissues (p < 10(-6)). In ord...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009790217407
更新日期：2009-01-01 00:00:00
abstract：:S100 binding protein A16 (S100A16) expression levels are closely associated with microRNA (miRNA) processing. Higher levels of S100A16 are reported during the progression of many cancers. Our study mainly explored the interaction between S100A16 and miR-6884-5p in gastric cancer (GC). Quantitative real-time polymerase...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504019X15753718797664
更新日期：2020-05-29 00:00:00
abstract：:ErbB4 is a member of the ErbB family of receptor tyrosine kinases. Because of a paucity of appropriate pharmacologic tools, little is known about ErbB4 functions in vivo. In response to this need, we hypothesized that a recombinant form of the extracellular domain of ErbB4 would antagonize ligand-induced receptor tyro...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/0965040042707907
更新日期：2004-01-01 00:00:00
abstract：:Human colon tumor cell lines that were adapted to grow in chemically defined medium were tested for their growth sensitivity to exogenous transferrin, insulin, and epidermal growth factor (EGF). Less differentiated cell lines, C and HCT116, were able to grow in the presence of a single peptide supplement, to respond s...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504013X13786659070316
更新日期：2013-01-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) are at the foundation of the hematopoietic hierarchy and give rise to all blood lineages in the adult organism. A thorough understanding of the molecular, cellular, and developmental biology of HSCs is of fundamental importance, but is also clinically relevant for the advancement of cel...
journal_title：Oncology research
pub_type： 杂志文章,评审
doi：10.3727/096504003108748519
更新日期：2003-01-01 00:00:00
abstract：:Inflammation plays a major role in the pathogenesis of cervical cancer. Chemokines are involved in inflammation, cancer, and infectious diseases. Therefore, we evaluated the association of the chemokine receptor gene polymorphism CCR5 Delta32 with risk of cervical cancer. A total of 150 histopathologically confirmed p...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008784523667
更新日期：2008-01-01 00:00:00
abstract：:There is an urgent need to improve the clinical management of hepatocellular carcinoma (HCC), one of the most common causes of global cancer-related deaths. Zingibar officinale is a medicinal herb used throughout history for both culinary and medicinal purposes. It has antioxidant, anticarcinogenic, and free radical s...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14816352324532
更新日期：2017-07-05 00:00:00
abstract：:Ahead of Print article withdrawn by publisher. ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14974821032421
更新日期：2017-06-15 00:00:00
abstract：:Baicalein, a flavonoid derived from the root of Scutellaria baicalensis, has been reported to possess multiple pharmacological activities, such as anticancer and anti-inflammatory properties. This study investigated the effect of baicalein in cervical cancer cells. Cell growth curve and MTT assay were performed and re...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15031557924141
更新日期：2018-05-07 00:00:00
abstract：:Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14666990347671
更新日期：2016-01-01 00:00:00
abstract：:Accumulating evidence indicates that microRNA-205 (miR-205) is involved in tumor initiation, development, and metastasis in various cancers. However, its functions in neuroblastoma (NB) remain largely unclear. Here we found that miR-205 was significantly downregulated in human NB tissue samples and cell lines. miR-205...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14974834436195
更新日期：2018-04-10 00:00:00
abstract：:A novel hydroquinone and NADH oxidase with protein disulfide-thiol interchange activity (designated ENOX2 or tNOX), associated exclusively with the outer leaflet of the plasma membrane at the surface of cancer cells and in sera of cancer patients, is absent from the surface of noncancer cells and from sera from health...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000007783630006
更新日期：2007-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is a disease with poor prognosis rates and ineffective therapeutic options. Previous studies have reported the involvement of mitogen-inducible gene 6 (MIG-6) as a negative regulator in tumor formation. MicroRNAs (miRNAs) play crucial roles in the development of different types of cancer...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15000784459799
更新日期：2018-05-07 00:00:00
abstract：:A majority of tumor cell lines are considered to be "methionine-dependent" because of their inability to grow in culture when methionine is replaced by its precursor, homocysteine. We have previously shown that this phenotype is not due to the incapacity to synthesize methionine from homocysteine but rather to a defic...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP/PBXIP1) is a nucleo-cytoplasmic shuttling protein, and its expression is associated with cancer aggressiveness. However, the role of HPIP in ovarian cancer is still unclear. Here, we aimed to clarify the role of HPIP in epithelial-m...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14575597858654
更新日期：2016-01-01 00:00:00
abstract：:The sequence of biochemical and molecular events that mediate growth arrest and cell death in tumor cells exposed to agents that induce DNA damage is poorly defined. This commentary exploits the recent explosion of information regarding oncogenes, tumor suppressor genes, and cell-cycle regulatory genes to develop a mo...
journal_title：Oncology research
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
abstract：:HL-60 cells isolated for resistance to vincristine are multidrug resistant and defective in the cellular accumulation of drug. Further studies demonstrate that these cells are also highly defective in 12-O-tetradecanoylphorbol-13-acetate (TPA) induced differentiation to macrophages. Analysis of this system demonstrate...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Electron-affinic compounds with strong DNA intercalating properties have demonstrated less than the expected radiosensitization due to restriction of their mobility along the DNA backbone and their lower extravascular diffusion in tumors. A 2-nitroimidazole linked 1,2,3,4-tetrahydroacridine derivative (THNLA-1) has be...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:We previously reported that vasoactive intestinal polypeptide (VIP) significantly inhibited Matrigel invasion and haptotactic migration of murine colon 26-L5 carcinoma in vitro. To extend our study, we investigated the inhibitory mechanisms of VIP on Matrigel invasion of colon 26-L5 carcinoma, and the effect on metast...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:A series of bis-aziridinylnaphthoquinone derivatives has been prepared. The cytotoxic activities and DNA alkylation abilities of these synthetic bis-aziridinylnaphthoquinone derivatives were investigated. They displayed significant cytotoxicity against human carcinomna cell lines and weak cytotoxic activities against ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:This commentary proposes the working hypothesis that transforming growth factor-beta (TGF-beta) can play two different and opposite roles with respect to the process of malignant progression. During the earliest stages of carcinogenesis, TGF-beta can act as potent tumor suppressor and may mediate the actions of chemop...
journal_title：Oncology research
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:The present study evaluated the modulatory effects of zinc on colonic membrane fluidity and surface abnormalities following 1,2 dimethylhydrazine (DMH)-induced colon carcinogenesis. Rats were segregated into four groups: normal control, DMH treated, zinc treated, DMH + zinc treated. Colon carcinogenesis was initiated ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009789745665
更新日期：2009-01-01 00:00:00
abstract：:The purpose of this study was to investigate the efficacy and safety of drug-eluting beads transarterial chemoembolization (DEB-TACE) treatment in Chinese hepatocellular carcinoma (HCC) patients and the prognostic factors for treatment response as well as survival. A total of 275 HCC patients were included in this pro...
journal_title：Oncology research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.3727/096504019X15662966719585
更新日期：2020-02-07 00:00:00
abstract：:The potential of American ginseng (AG) ( Panax quinquefolium), a commonly used herbal remedy believed to have anticarcinogenic effects, to prevent the development of mammary tumors was evaluated in a mouse model of dimethylbenzanthracene (DMBA)-induced mammary carcinoma. Ginsenosides, believed to be the active compone...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000006783981017
更新日期：2006-01-01 00:00:00
