Abstract：
:Human colon tumor cell lines that were adapted to grow in chemically defined medium were tested for their growth sensitivity to exogenous transferrin, insulin, and epidermal growth factor (EGF). Less differentiated cell lines, C and HCT116, were able to grow in the presence of a single peptide supplement, to respond synergistically to transferrin and insulin in combination, and to be insensitive to supplementation with exogenous EGF. The more differentiated cell lines, CBS and GEO, were found to respond to exogenous EGF in a concentration-dependent manner, to require at least two peptide factor supplements to support growth, and to respond synergistically when EGF was added to chemically defined medium that already contained transferrin and insulin. To investigate whether changes in the number or the affinity-classes of the EGF-receptor might be involved in any of these growth responses, changes in EGF-receptor number and dissociation constant were determined as a function of cell growth condition. The poorly differentiated HCT cell line HCT116 was found to undergo 28 to 64% increases in [125I]EGF-binding when its medium was supplemented with transferrin, insulin, or transferrin plus insulin. The more differentiated CBS cell line responded to all peptide supplements with decreases in [125I]EGF-binding ranging from 12 to 73%. These findings indicate that the actions of transferrin and insulin are fundamental to the growth regulatory mechanisms of these two differentiation classes of human colon tumor cell lines, but that their mechanisms are different.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Yeoman LC,Wan CW,Zorbas MAsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
273-9issue
7-8eissn
0965-0407issn
1555-3906journal_volume
8pub_type
杂志文章abstract：:Spinal osteosarcoma (OS) is a malignant tumor that has a poor outcome. MicroRNA-520b (miR-520b) acts as a cancer suppressor in various types of cancer. Because of the limited amount of literature on OS, we aimed to identify the role of miR-520b in OS. The miR-520b level in clinical spinal OS tissues and adjacent nontu...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14873430389189
更新日期：2017-09-21 00:00:00
abstract：:Our recent studies demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) has pharmacological activity for the treatment of acute myelocytic leukemia patients. In the present study, we investigated the potential synergistic effect of all-trans retinoic acid (RA), 1alpha,25-dihydroxyvitamin D3 (VD3), and sodium b...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Cervical cancer is one of the most common neoplastic diseases affecting women, with a combined worldwide incidence of almost half a million new cases. Considering the fact that IL-18 plays an important role in the interactions among T cells, NK cells, and macrophages and induces IFN-gamma production, efforts should be...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008785114156
更新日期：2008-01-01 00:00:00
abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is a DNA repair protein that reverses alkylation damage produced by chloroethylnitrosoureas and is a major determinant of cellular resistance to adjuvant chemotherapy with these drugs. AGT activity was measured in 119 samples from 69 patients, including normal, tumor, and dis...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Neuroendocrine tumors (NETs) represent a relatively rare form of neoplasias for which, in advanced unresectable stages, palliative treatment options remain limited largely to the use of somatostatin analogues or chemotherapy. Several newer targeted therapeutic options, alone or in combination with somatostatin analogu...
journal_title：Oncology research
pub_type： 杂志文章,评审
doi：10.3727/096504011x13123323849799
更新日期：2011-01-01 00:00:00
abstract：:Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504013X13786659070316
更新日期：2013-01-01 00:00:00
abstract：:Carbonic anhydrase (CA) IX has emerged as a promising target for cancer therapy. It is highly upregulated in hypoxic regions and mediates pH regulation critical for tumor cell survival as well as extracellular acidification of the tumor microenvironment, which promotes tumor aggressiveness via various mechanisms, such...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14965111926391
更新日期：2018-03-05 00:00:00
abstract：:We previously reported that vasoactive intestinal polypeptide (VIP) significantly inhibited Matrigel invasion and haptotactic migration of murine colon 26-L5 carcinoma in vitro. To extend our study, we investigated the inhibitory mechanisms of VIP on Matrigel invasion of colon 26-L5 carcinoma, and the effect on metast...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Recently, microRNA (miR)-33b has been demonstrated to act as a tumor suppressor in osteosarcoma. However, the regulatory mechanism of miR-33b in osteosarcoma cell proliferation and migration remains largely unknown. In this study, real-time PCR showed that miR-33b was significantly downregulated in osteosarcoma tissue...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14743337535446
更新日期：2017-03-13 00:00:00
abstract：:MicroRNAs (miRNAs) are emerging as pivotal regulators in the development and progression of various cancers, including renal cell carcinoma (RCC). MicroRNA-384 (miR-384) has been found to be an important cancer-related miRNA in several types of cancers. However, the role of miR-384 in RCC remains unclear. In this stud...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15035025554553
更新日期：2018-04-10 00:00:00
abstract：:Signal peptide CUB EGF-like domain-containing protein 2 (SCUBE2), a member of the SCUBE family of proteins, was recently found to play an important role in cancer development. However, little is known regarding its biological function in glioma. In the present study, we investigated the effect of SCUBE2 on glioma and ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14747335734344
更新日期：2017-03-13 00:00:00
abstract：:Cyclin Y (CCNY) is a novel cyclin and almost nothing is known about its expression level and role in human cancers. In the present study, we found that lentivirus-mediated RNA interference (RNAi) of CCNY significantly downregulated its expression level. More importantly, knockdown of CCNY inhibited cell proliferation,...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504010x12644422320582
更新日期：2010-01-01 00:00:00
abstract：:Lung cancer is a lethal solid tumor with poor prognosis because of its high metastasis and resistance to current therapies. Recently, cancer stem cells (CSCs) were suggested to be major contributors to tumorigenicity and cancer relapse. However, therapeutic targets for lung cancer-related CSCs remain undetermined. The...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504013X13685487925095
更新日期：2013-01-01 00:00:00
abstract：:The melanin biosynthetic pathway from tyrosine is a potential target for combating malignant melanoma. N-Acetyl-4-S-cysteaminylphenol 1 is a previously synthesized analogue of tyrosine that probably acts by this pathway. It interferes with cell growth and proliferation via selective oxidation in melanocytes to an oqui...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000006783981206
更新日期：2006-01-01 00:00:00
abstract：:Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary hu...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14719078133168
更新日期：2017-01-02 00:00:00
abstract：:Numerous data indicate that cellular oxidoreductases may be responsible for the cardiotoxic effects of antitumor anthracycline drugs as a consequence of the mediation by these agents of one-electron transfer from reduced nucleotides to atmospheric oxygen. This process is catalyzed primarily by NADH dehydrogenase, NADP...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/0965040042380450
更新日期：2004-01-01 00:00:00
abstract：:Phytochemicals present in tea, particularly polyphenols, have anticancer properties against several cancer types. However, studies elucidating the role and the mechanism(s) of action of tea polyphenols in cervical cancer are sparse. In this study, we investigated the mechanism of antiproliferative and apoptotic action...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504011x13021877989711
更新日期：2011-01-01 00:00:00
abstract：:We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injec...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504013x13639794277680
更新日期：2013-01-01 00:00:00
abstract：:Inflammation plays a major role in the pathogenesis of cervical cancer. Chemokines are involved in inflammation, cancer, and infectious diseases. Therefore, we evaluated the association of the chemokine receptor gene polymorphism CCR5 Delta32 with risk of cervical cancer. A total of 150 histopathologically confirmed p...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008784523667
更新日期：2008-01-01 00:00:00
abstract：:Osteosarcoma is the most common primary bone malignancy manifested predominantly in children and young adults. Studies indicate that miR-107 is involved in the pathogenesis of osteosarcoma and that tropomyosin 1 (TPM1) acts as a tumor suppressor in many types of cancer. In this study, we analyzed the effect of miR-107...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14882829077237
更新日期：2017-09-21 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is the third leading cause of cancer-associated mortality worldwide. Although the mechanisms of HCC progression are not well understood, recent studies demonstrated the potential contribution of uric acid transporter SLC2A9 to tumor suppression. However, the roles and underlying mechanis...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15199489058224
更新日期：2019-05-07 00:00:00
abstract：:Kaempferol is a flavonoid that has been extensively investigated owing to its antitumor effects. Nevertheless, little is known about its underlying mechanisms of action. We aimed to explore the role of kaempferol in breast cancer (BC), and thus we investigated how kaempferol suppresses the growth of BC cells. The cell...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15228018559434
更新日期：2019-06-21 00:00:00
abstract：:Metaplastic tubular complexes (MTC) have been proposed as precursor lesions for pancreatic adenocarcinoma (PDAC). In this study, we investigated the potential role of bone marrow-derived progenitor cells (BMPC) in the formation of MTC and PDAC in a rat model. F344 rats defective for CD26 (dipeptidyl peptidase IV, DPPI...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009788428424
更新日期：2009-01-01 00:00:00
abstract：:We investigated the regulatory effect of interleukin (IL) 10 on IL-12-inducible killer activity of blood mononuclear cells (MNC) of healthy subjects. IL-10 suppressed non-MHC-restricted killer activity induced by IL-12 in a dose-dependent manner. Cytotoxicity against Daudi cells of blood MNC activated with optimal dos...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Medullary thyroid carcinoma (MTC) is an uncommon and highly aggressive tumor of the neuroendocrine system, which derives from the neuroendocrine C cells of the thyroid gland. Except for surgical resection, there are not very many effective systemic treatment options for MTC. N-Myc downstream-regulated gene 2 (NDRG2) h...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504015X14452563486093
更新日期：2016-01-21 00:00:00
abstract：:Constitutive canonical Wnt signaling, resulting from mutations in the adenomatous polyposis coli (APC), beta-catenin, or axin genes, has been implicated in the initiation of most human colorectal cancers (CRCs). Some of the proposed approaches for CRC prevention and treatment involve the downregulation of canonical Wn...
journal_title：Oncology research
pub_type： 杂志文章,评审
doi：10.3727/096504008784046108
更新日期：2008-01-01 00:00:00
abstract：:Triptorelin, a kind of GnRH agonist, is widely used in the treatment of hormone-responsive cancers in the clinic. This study aimed to discover the underlying mechanism of triptorelin in protection from 5-fluorouracil (5-FU)-induced ovarian damage in Sprague-Dawley rats. In the present study, after using 5-FU to induce...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504015X14410238486720
更新日期：2014-01-01 00:00:00
abstract：:Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) (p16INK4A/MTS1) tumor suppressor gene has been found in a variety of human tumor types. To investigate the involvement of CDKI abnormality in mesenchymal chondrosarcoma, alterations of CDKIs were examined in human mesenchymal chondrosarcoma tiss...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000001108747381
更新日期：2000-01-01 00:00:00
abstract：:We investigated the susceptibility of three inbred strains of rats to the hepatocarcinogen, N-nitrosomorpholine (NNM), to establish a spontaneous metastatic model of hepatocellular carcinoma (HCC). WS/Shi. SD/gShi, and F344/DuCrj rats were given 0.02% NNM in drinking water for 8 weeks and thereafter left without any t...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504001108747594
更新日期：2000-01-01 00:00:00
abstract：:To investigate the effect of Kim-1 on 786-0 cells in vivo and in vitro, several experiments such as quantitative real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with Kim-1 siRNA. Furthermore, the tumor xenograft model was ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15140544654946
更新日期：2018-08-23 00:00:00
