Abstract：
:We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injecting with MCF-7 cells, and were randomized into four groups when palpable tumors reached 190 mm3. The length and width of MCF-7 tumors in mice were measured every 3 days. After 20 days, all mice were killed and the expression of VEGF-C in tumor tissue was also detected by Western blot and qRT-PCR. Results showed that VEGF-C siRNA effectively suppressed the protein and mRNA expression of VEGF-C in MCF-7 cells in vitro. VEGF-C siRNA inhibited the growth of human lymphatic endothelial cells (LECs) and MCF-7 cells. The volume and weight of MCF-7 tumor in VEGF-C siRNA microbubble with irradiation group were reduced with more extent than that in other groups in vivo. The present study highlights that VEGF-C siRNA in combination with ultrasound-mediated microbubble destruction (UMMD) could be a powerful, promising nonviral technology for breast cancer gene therapy.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Xu Q,Sun T,Tian H,Wang C,Zhou Hdoi
10.3727/096504013x13639794277680subject
Has Abstractpub_date
2013-01-01 00:00:00pages
297-301issue
7eissn
0965-0407issn
1555-3906journal_volume
20pub_type
杂志文章abstract：:Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC ce...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14685034103950
更新日期：2016-10-27 00:00:00
abstract：:The aim of this study was to analyze the efficacy according to EGFR status and predictors of TKIs in Chinese advanced lung adenocarcinoma patients in a single institute. We retrospectively enrolled 253 patients with advanced or recurrent adenocarcinoma and history of EGFR-TKI treatment attended at Beijing Chest Hospit...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504014X13907540404833
更新日期：2014-01-01 00:00:00
abstract：:Drug resistance is the major obstacle for the successful therapy of lung adenocarcinoma. It was suggested that ß-elemene, a major isoform of elemene, could reverse the drug resistance in lung cancer cells. However, the underlying mechanisms remains poorly known. Here, we aimed to investigate whether elemene is involve...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14954936991990
更新日期：2017-05-23 00:00:00
abstract：:Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP/PBXIP1) is a nucleo-cytoplasmic shuttling protein, and its expression is associated with cancer aggressiveness. However, the role of HPIP in ovarian cancer is still unclear. Here, we aimed to clarify the role of HPIP in epithelial-m...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14575597858654
更新日期：2016-01-01 00:00:00
abstract：:To ascertain whether insulin-like growth factors (IGF-1 and IGF-2) affect the estrogen- and insulin-responsive R3230AC mammary carcinoma, studies of IGF-1 and IGF-2 receptors were conducted on primary-culture tumor cells and on membranes purified from whole tumors. By saturation binding analysis, cells in culture disp...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:The patterns of DNA fragmentation were evaluated following a brief exposure (2 h) of the human ileocecal adenocarcinoma cell line, HCT-8, to several specific thymidylate synthase inhibitors, a quinazoline (ZD1694) and benz[cd]indole-containing molecule (AG-331). The magnitude and size of DNA fragmentation induced by t...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Venous thromboembolism (VTE) occurs at an increased incidence in cancer patients. A cancer-related hypercoagulable state has been considered to play role in this phenomenon. Preclinical data suggest an association between tumor expression of MET proto-oncogene (MET) and a hypercoagulable state, resulting in VTE. We in...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504010x12828372551786
更新日期：2010-01-01 00:00:00
abstract：:Lung cancer is the leading cause of deaths due to cancer. Studies suggest an important role of microRNAs (miRNAs) in a variety of cancers, including lung cancer. In the present study, we evaluated the role of miR-641 in human lung cancer A549 cells. Quantitative RT-PCR and Western blot were used to measure mRNA and pr...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15021536183490
更新日期：2018-06-11 00:00:00
abstract：:3'-Azido-3'-deoxythymidine (AZT) competitively inhibited the transport of thymidine (Km = 0.23 mM) into human erythrocytes with a Ki of 1.0 mM at 37 degrees C. The principal human metabolite of AZT in plasma, the 5'-glucuronide (GAZT), was a weak inhibitor of the nucleoside transporter (< 20% inhibition of the influx ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Laryngeal cancer, one of the most common head and neck malignancies, is an aggressive neoplasm. Increasing evidence has demonstrated that microRNAs (miRNAs) exert important roles in oncogenesis and progression of diverse types of human cancers. miR-632, a tumor-related miRNA, has been reported to be dysregulated and i...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15213142076069
更新日期：2020-02-07 00:00:00
abstract：:Solid tumors involving glandular organs express mucin glycoprotein that is eventually shed into the circulation. As a result, these proteins can easily be measured in the serum and be used as potential tumor markers. The most commonly used tumor markers for breast cancer are CA27-29 and CA15-3, which both measure the ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15128550060375
更新日期：2018-04-10 00:00:00
abstract：:Protein disulfide isomerases A6 (PDIA6) belongs to the PDI family. Recently, PDIA6 was found to have a close association with various cancers. However, there has been little investigation into the biological functions of PDIA6 in bladder cancer (BC). In this study, we explored the expression pattern and functional sig...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14761811155298
更新日期：2017-04-14 00:00:00
abstract：:Angiogenesis, the formation of new vessels, is essential for tumor growth and metastasis. Mutations of p53 tumor suppressor gene are frequent and play an important role in colorectal oncogenesis. A role of p53 as an angiogenesis inhibitor has also been proposed. We evaluated angiogenesis and p53 expression in 16 hyper...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504001108747693
更新日期：2000-01-01 00:00:00
abstract：:Inflammation plays a major role in the pathogenesis of cervical cancer. Chemokines are involved in inflammation, cancer, and infectious diseases. Therefore, we evaluated the association of the chemokine receptor gene polymorphism CCR5 Delta32 with risk of cervical cancer. A total of 150 histopathologically confirmed p...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504008784523667
更新日期：2008-01-01 00:00:00
abstract：:Metaplastic tubular complexes (MTC) have been proposed as precursor lesions for pancreatic adenocarcinoma (PDAC). In this study, we investigated the potential role of bone marrow-derived progenitor cells (BMPC) in the formation of MTC and PDAC in a rat model. F344 rats defective for CD26 (dipeptidyl peptidase IV, DPPI...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009788428424
更新日期：2009-01-01 00:00:00
abstract：:To investigate the effect of Kim-1 on 786-0 cells in vivo and in vitro, several experiments such as quantitative real-time PCR, Western blot, MTT, colony formation, and flow cytometry were performed to evaluate the biological behavior of 786-0 cells treated with Kim-1 siRNA. Furthermore, the tumor xenograft model was ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X15140544654946
更新日期：2018-08-23 00:00:00
abstract：:Our recent studies have shown that vanadium, a dietary micronutrient, has an inhibitory response against experimentally induced rat liver carcinogenesis. In the present study, the effect of vanadium on hepatic xenobiotic biotransformation in rats exposed to diethylnitrosamine (DENA, 200 mg/kg, IP) was investigated to ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Kiss-1 has been identified as a putative metastasis suppressor gene in various human malignancies. However, there is little information about its possible role in gastric carcinoma. In this study, we determined whether the Kiss-1 gene negatively regulates MMP-9 expression. cDNA microarray technology was used to identi...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009789954591
更新日期：2009-01-01 00:00:00
abstract：:A novel keto-diepoxide Sch 49209 and its derivative Sch 50672, produced by the fungus Nattrassia mangiferae, inhibited tumor cell invasion through an artificial basement membrane. These compounds, at nontoxic concentrations, inhibited invasion of HT-1080 cells in a dose-dependent manner. The IC50 values for Sch 49209 ...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Thymosin-beta-4 (TB4) as an actin-sequestering peptide has been detected outside of cells in blood plasma or in wound fluid. TB4 induces tumor metastasis and paclitaxel resistance, which is the most significant obstacle to successful therapy in tumors. Here we investigated the inhibitory effect of TB4 peptides on tumo...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504007783438349
更新日期：2007-01-01 00:00:00
abstract：:Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14841698396829
更新日期：2017-08-07 00:00:00
abstract：:Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNA...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14813859905646
更新日期：2017-07-05 00:00:00
abstract：:We investigated the regulatory effect of interleukin (IL) 10 on IL-12-inducible killer activity of blood mononuclear cells (MNC) of healthy subjects. IL-10 suppressed non-MHC-restricted killer activity induced by IL-12 in a dose-dependent manner. Cytotoxicity against Daudi cells of blood MNC activated with optimal dos...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The present study evaluated the modulatory effects of zinc on colonic membrane fluidity and surface abnormalities following 1,2 dimethylhydrazine (DMH)-induced colon carcinogenesis. Rats were segregated into four groups: normal control, DMH treated, zinc treated, DMH + zinc treated. Colon carcinogenesis was initiated ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009789745665
更新日期：2009-01-01 00:00:00
abstract：:A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist showed cell death in th...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14721731148893
更新日期：2017-04-14 00:00:00
abstract：:The importance of receptors involved in the clearance of proteases and protease/inhibitor complexes in tumor invasion is unknown. We studied the expression of the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor (LRP/alpha 2-MR), the major receptor involved in the clearance of protease/i...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The aim of this study was to assess serum levels of vascular endothelial growth factor C and D (VEGF-C, VEGF-D) and soluble VEGF receptor 2 (sVEGFR-2) in patients with lung cancer during chemotherapy. The study included 80 patients (64 men and 16 women; mean age 61.1) diagnosed histologically with lung cancer. Forty-f...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Pancreatic cancer (PC) is a devastating malignant disease with a poor prognosis. This study aimed to investigate the role of urothelial carcinoma associated 1 (UCA1) in the progression of PC. Our results revealed that long noncoding RNA (lncRNA) UCA1 was overexpressed in PC tissues compared with adjacent histologicall...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14888987683152
更新日期：2017-11-02 00:00:00
abstract：:Gastric cancer (GC) is the most common epithelial malignancy worldwide. Basic transcription factor 3 (BTF3) plays a crucial role in the regulation of various biological processes. We designed experiments to investigate the molecular mechanism underlying the role of BTF3 in GC cell proliferation and metastasis. We conf...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504017X14886494526344
更新日期：2017-11-02 00:00:00
abstract：:Recently, microRNA (miR)-33b has been demonstrated to act as a tumor suppressor in osteosarcoma. However, the regulatory mechanism of miR-33b in osteosarcoma cell proliferation and migration remains largely unknown. In this study, real-time PCR showed that miR-33b was significantly downregulated in osteosarcoma tissue...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14743337535446
更新日期：2017-03-13 00:00:00
