Abstract：
:Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC cell lines established from primary HCC tumors, we found that CD133-high human liver cancer stem-like cells (CD133hi) derived from the SNU-475 cell line were highly susceptible to TRAIL compared to other HCC cell lines with a small population of CD133. CD133hi SNU-475 cells showed upregulation of TRAIL receptor DR5 and stemness-related genes such as c-Myc and ABC transporters compared to their CD133-low (CD133lo) cells. Hypersensitivity of CD133hi cells to TRAIL was associated with c-Myc-mediated upregulation of DR5 and downregulation of c-FLIPL in the cells. Knockdown of CD133 expression in CD133hi cells resulted in the downregulation of c-Myc, and depletion of c-Myc caused a decrease in the cell surface expression of DR5 and an increase in the expression of c-FLIPL and, consequently, attenuated TRAIL-induced cytotoxicity and apoptosis of CD133hi cells. These results suggest that TRAIL may provide a new strategy for CD133hi CSCs of HCC-targeted therapies and, potentially, for therapies of other CD133-expressing types of cancer.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Lee SH,Hyun SK,Kim HB,Kang CD,Kim SHdoi
10.3727/096504016X14685034103950subject
Has Abstractpub_date
2016-10-27 00:00:00pages
495-509issue
6eissn
0965-0407issn
1555-3906journal_volume
24pub_type
杂志文章abstract：:Topotecan, a topoisomerase I poison and water-soluble derivative of camptothecin, has shown promise in treating solid tumors; however, the drug is unstable under physiological conditions and converts to an inactive form within 30 minutes. Encapsulating topotecan in liposomes (LIP-TPT) minimizes inactivation. The effic...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The ability of mitoxantrone to form DNA adducts was investigated in a series of human tumor cell lines consisting of human cervical cancer (HeLa), human breast cancer (MCF-7), and human neuroblastoma (IMR-32) cells. The mitoxantrone-resistant human promyelocytic leukemia cell line HL60/MX2 was also compared to the par...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504003773994815
更新日期：2004-01-01 00:00:00
abstract：:The potency of flavonoids (isoflavones, flavones, and flavanones) to inhibit efflux of 2',7'-bis-(carboxypropyl)-5(6)-carboxyfluorescein (BCPCF) from human erythrocytes was investigated. Structure-activity relationship analysis showed that the strongest inhibitors were found among flavanones bearing a hydrophobic pren...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000003108747983
更新日期：2003-01-01 00:00:00
abstract：:MicroRNAs (miRNAs) are endogenous small noncoding RNAs that negatively regulate gene expression at the posttranscriptional level and play an important role in carcinogenesis. Herein, we characterized the global expression of miRNA in distal gastric adenocarcinomas and determined if circulating miRNAs could be used as ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504012x13522227232156
更新日期：2012-01-01 00:00:00
abstract：:Medullary thyroid carcinoma (MTC) is an uncommon and highly aggressive tumor of the neuroendocrine system, which derives from the neuroendocrine C cells of the thyroid gland. Except for surgical resection, there are not very many effective systemic treatment options for MTC. N-Myc downstream-regulated gene 2 (NDRG2) h...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504015X14452563486093
更新日期：2016-01-01 00:00:00
abstract：:A number of previous investigations have reported that physical exercise renders immunopotentiating and antitumor therapeutic benefits to the tumor-bearing host. As these effects of physical exercise are mainly mediated through the modulation of hormonal and cytokine repertoire, it remains unclear if male and female t...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504010x12629634366142
更新日期：2010-01-01 00:00:00
abstract：:Ameloblastoma is the most frequently encountered odontogenic tumor, characterized by a locally invasive behavior, frequent recurrences, and, although rare, metastatic capacity. Loss or inactivation of tumor suppressor genes (TSGs) allows cells to acquire neoplastic growth. The ING family proteins are tumor suppressors...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504010x12704916124864
更新日期：2010-01-01 00:00:00
abstract：:The importance of receptors involved in the clearance of proteases and protease/inhibitor complexes in tumor invasion is unknown. We studied the expression of the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor (LRP/alpha 2-MR), the major receptor involved in the clearance of protease/i...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The pre-mRNA splicing regulator serine-arginine protein kinase 1 (SRPK1), a member of the SR kinase family, plays an essential role in cancer development and various pathophysiological processes. However, its expression pattern and functions in renal cell carcinoma (RCC) remain unknown. Therefore, the aim of this stud...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14743324568129
更新日期：2017-03-13 00:00:00
abstract：:Tamoxifen causes a mitochondrial transmembrane potential dysfunction and ATP depletion, which may play a role in tamoxifen cytotoxicity. Administration of oligomycin-2 deoxy glucose (2DG) enhanced tamoxifen antiproliferative effects, which may be due to exacerbated ATP depletion following tamoxifen and oligomycin-2DG ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504009x12596189659204
更新日期：2009-01-01 00:00:00
abstract：:Ubiquitin-specific protease 22 (USP22), a novel deubiquitinating enzyme, belongs to an extended family of proteins that have ubiquitin hydrolase activity. Recently, USP22 has attracted widespread attention because of its implication in carcinogenesis. However, there have been no studies, to our knowledge, investigatin...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14772395226335
更新日期：2017-05-24 00:00:00
abstract：:Synthesis of poly(gamma-glutamyl) metabolites of many antifolates, such as methotrexate (MTX), by folylpolyglutamate synthetase (FPGS) is often essential to their cytotoxic activity. FPGS expression in the MTX-sensitive human T-lymphoblastic leukemia cell line CCRF-CEM and a number of MTX-resistant sublines was previo...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Human papillomavirus type 16 (HPV16) is a major causative factor in the development of uterine cervical carcinomas. We investigated the role of E6/E7 in tumor formation. Skin-specific E6/E7 transgenic mice showed approximately twice as many tumors compared with nontransgenic mice in dimethylbenz[a]anthracene (DMBA)-in...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000006783980964
更新日期：2007-01-01 00:00:00
abstract：:The human nucleolar protein p120 is highly expressed in human cancers. Its high expression in breast cancer correlates with a poor prognosis, and its overexpression in 3T3 mouse fibroblasts causes malignant transformation. This study reports that a combination of monoclonal anti-p 120 antibody (MAbp120), liposomes (Li...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Numerous data indicate that cellular oxidoreductases may be responsible for the cardiotoxic effects of antitumor anthracycline drugs as a consequence of the mediation by these agents of one-electron transfer from reduced nucleotides to atmospheric oxygen. This process is catalyzed primarily by NADH dehydrogenase, NADP...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/0965040042380450
更新日期：2004-01-01 00:00:00
abstract：:ErbB4 is a member of the ErbB family of receptor tyrosine kinases. Because of a paucity of appropriate pharmacologic tools, little is known about ErbB4 functions in vivo. In response to this need, we hypothesized that a recombinant form of the extracellular domain of ErbB4 would antagonize ligand-induced receptor tyro...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/0965040042707907
更新日期：2004-01-01 00:00:00
abstract：:This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apoptotic rate, as wel...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14685034103239
更新日期：2016-10-27 00:00:00
abstract：:Progestin and adipoQ receptor family member III (PAQR3), a member of the PAQR family, is frequently downregulated in different types of human cancer. However, its expression and functions in esophageal cancer are still unknown. This study aimed to explore the expression of PAQR3 in esophageal cancer cell lines and to ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14761384026719
更新日期：2017-05-24 00:00:00
abstract：:A1, a member of the Bcl-2 gene family, was originally identified as a hemopoietic-specific early response gene. Later it was found that A1 was overexpressed in human stomach cancer tissues and was induced by tumor necrosis factor-alpha (TNF-alpha) in human vascular endothelial cells. However, its expression in human c...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:We applied a differential display method to screen mRNAs isolated from a newly established cell line that carried a wild-type p53 transgene under control of the lactose operon. To investigate the p53 signaling pathway, we looked for genes whose expression was significantly induced or suppressed by induction of wild-ty...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Accumulated studies have strongly implicated aberrantly expressed microRNAs (miRNAs) in carcinogenesis and cancer progression of various cancers, including colorectal cancer (CRC). Hence, a comprehensive study of miRNAs and their association with CRC may be a promising therapeutic method for patients with this maligna...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15188747585738
更新日期：2018-10-17 00:00:00
abstract：:Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) (p16INK4A/MTS1) tumor suppressor gene has been found in a variety of human tumor types. To investigate the involvement of CDKI abnormality in mesenchymal chondrosarcoma, alterations of CDKIs were examined in human mesenchymal chondrosarcoma tiss...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/000000001108747381
更新日期：2000-01-01 00:00:00
abstract：:Pathophysiological factors exist within solid tumors that lead to a low pH environment. Therefore, pH-sensitive cancer chemotherapeutic agents may selectively target and kill tumor cells while sparing the normal tissue from toxicity. Using colony forming assays to assess cell survival, we found that EMT6 mouse mammary...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Small-cell lung cancer (SCLC) has a significantly worse prognosis than other forms of bronchogenic carcinoma. Because transforming growth factor beta (TGF-beta) appears to play an important role in the pathogenesis of SCLC, we examined the status of the TGF-beta receptor system in a series of 11 human small-cell carci...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Empty spiracles homeobox 2 (EMX2) is a homeodomain-containing transcription factor that plays an essential role in tumorigenesis. However, to the best of our knowledge, the role of EMX2 in human colorectal cancer (CRC) is still unclear. Thus, the aim of this study was to investigate the expression and role of EMX2 in ...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14756640150695
更新日期：2017-04-14 00:00:00
abstract：:Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504016X14666990347671
更新日期：2016-01-01 00:00:00
abstract：:We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injec...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504013x13639794277680
更新日期：2013-01-01 00:00:00
abstract：:The mechanism of action of the anthraquinone antileukemic drug mitoxantrone (MIT) was investigated and compared with that of daunorubicin (DNR) with emphasis on the interaction with DNA to clarify the more potent cytotoxic activity of MIT than DNR in human leukemia cells. MIT showed similar characteristics to those of...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Sixteen desmuramyl analogs of muramyl dipeptide (MDP) were tested for their abilities to stimulate cytotoxic factor secretion by mouse peritoneal macrophages. Among them, the pseudohexapeptide Boc-Gly psi [CH2O]-D-Ala-Ala-D-Glu[Lys(H-Gly)NHEt]-NH2 appeared to be four times more effective than MDP. From this study, the...
journal_title：Oncology research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Human glioblastoma multiforme (GBM) accounts for the majority of human brain gliomas. Several TMEM proteins, such as TMEM 45A, TMEM 97, and TMEM 140, are implicated in human brain gliomas. However, the roles of TMEM168 in human GBM remain poorly understood. Herein we found that mRNA levels of TMEM168 were overexpresse...
journal_title：Oncology research
pub_type： 杂志文章
doi：10.3727/096504018X15478559215014
更新日期：2019-07-12 00:00:00
