Abstract:
:Synthesis of poly(gamma-glutamyl) metabolites of many antifolates, such as methotrexate (MTX), by folylpolyglutamate synthetase (FPGS) is often essential to their cytotoxic activity. FPGS expression in the MTX-sensitive human T-lymphoblastic leukemia cell line CCRF-CEM and a number of MTX-resistant sublines was previously investigated at the DNA, RNA, and activity levels. Using an FPGS peptide deduced from its cDNA sequence, a rabbit polyclonal antibody to FPGS has now been elicited, immunoaffinity purified, and used to quantitate FPGS protein expression by chemiluminescent Western immunoblot analysis. The antibody was used to determine the half-life of human FPGS protein (3.7 +/- 1.1 h) in parental CCRF-CEM cells. A subline resistant to MTX as a result of amplified dihydrofolate reductase expression shows no change in FPGS protein or activity relative to CCRF-CEM. An MTX transport-defective line, however, displays both higher FPGS protein and activity levels. For several sublines in which the only apparent mechanism of MTX resistance is decreased FPGS activity, the FPGS protein level is decreased proportionally. However, we previously showed that these sublines have the same gene copy number, restriction map, and mRNA size and levels as the parent. Evidently, in these MTX-resistant sublines the mRNA is poorly translated and/or the protein turns over more rapidly.
journal_name
Oncol Resjournal_title
Oncology researchauthors
McGuire JJ,Russell CAsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
193-200issue
4eissn
0965-0407issn
1555-3906journal_volume
10pub_type
杂志文章abstract::Accumulating evidence indicates that microRNA-205 (miR-205) is involved in tumor initiation, development, and metastasis in various cancers. However, its functions in neuroblastoma (NB) remain largely unclear. Here we found that miR-205 was significantly downregulated in human NB tissue samples and cell lines. miR-205...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14974834436195
更新日期:2018-04-10 00:00:00
abstract::A1, a member of the Bcl-2 gene family, was originally identified as a hemopoietic-specific early response gene. Later it was found that A1 was overexpressed in human stomach cancer tissues and was induced by tumor necrosis factor-alpha (TNF-alpha) in human vascular endothelial cells. However, its expression in human c...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Dysregulation of SUMO-specific protease 1 (SENP1) expression has been reported in several kinds of cancer, including human colorectal and prostate cancers, proposing SENP1 as an oncogene with a critical role in cancer progression. miR-133a-3p has been reported as a tumor suppressor in several malignant neoplasias. How...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15004613574679
更新日期:2018-06-11 00:00:00
abstract::The patterns of DNA fragmentation were evaluated following a brief exposure (2 h) of the human ileocecal adenocarcinoma cell line, HCT-8, to several specific thymidylate synthase inhibitors, a quinazoline (ZD1694) and benz[cd]indole-containing molecule (AG-331). The magnitude and size of DNA fragmentation induced by t...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Human colon tumor cell lines that were adapted to grow in chemically defined medium were tested for their growth sensitivity to exogenous transferrin, insulin, and epidermal growth factor (EGF). Less differentiated cell lines, C and HCT116, were able to grow in the presence of a single peptide supplement, to respond s...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::This commentary proposes the working hypothesis that transforming growth factor-beta (TGF-beta) can play two different and opposite roles with respect to the process of malignant progression. During the earliest stages of carcinogenesis, TGF-beta can act as potent tumor suppressor and may mediate the actions of chemop...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are emerging as pivotal regulators in the development and progression of various cancers, including renal cell carcinoma (RCC). MicroRNA-384 (miR-384) has been found to be an important cancer-related miRNA in several types of cancers. However, the role of miR-384 in RCC remains unclear. In this stud...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15035025554553
更新日期:2018-04-10 00:00:00
abstract::Phytochemicals present in tea, particularly polyphenols, have anticancer properties against several cancer types. However, studies elucidating the role and the mechanism(s) of action of tea polyphenols in cervical cancer are sparse. In this study, we investigated the mechanism of antiproliferative and apoptotic action...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504011x13021877989711
更新日期:2011-01-01 00:00:00
abstract::Signal peptide CUB EGF-like domain-containing protein 2 (SCUBE2), a member of the SCUBE family of proteins, was recently found to play an important role in cancer development. However, little is known regarding its biological function in glioma. In the present study, we investigated the effect of SCUBE2 on glioma and ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14747335734344
更新日期:2017-03-13 00:00:00
abstract::Epithelial ovarian cancer (EOC) has remained an enigmatic disease, the etiology of which is mostly unknown. Considering the age incidence around menopause, a "gonadotrophin theory" has been proposed, and considering the association with infertility, an "incessant ovulation" theory has been proposed. EOC originates fro...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:10.3727/096504008786111383
更新日期:2008-01-01 00:00:00
abstract::We previously reported that 9-methylstreptimidone, a piperidine compound isolated from a culture filtrate of Streptomyces, induces apoptosis selectively in adult T-cell leukemia cells. It was screened for a compound that inhibits LPS-induced NF-kappaB and NO production in mouse macrophages. However, 9-methystreptimido...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504012x13425470196056
更新日期:2012-01-01 00:00:00
abstract::Tamoxifen causes a mitochondrial transmembrane potential dysfunction and ATP depletion, which may play a role in tamoxifen cytotoxicity. Administration of oligomycin-2 deoxy glucose (2DG) enhanced tamoxifen antiproliferative effects, which may be due to exacerbated ATP depletion following tamoxifen and oligomycin-2DG ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504009x12596189659204
更新日期:2009-01-01 00:00:00
abstract::Genistein is an inhibitor of the enzymes protein tyrosine kinase and topoisomerase-II. It induces G2-phase arrest in human Jurkat and murine P388 leukemia cells at concentrations at which it is also cytotoxic. The effects of genistein have been investigated on Jurkat and P388 leukemia sublines that manifest multidrug ...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Mutations in the APC gene contribute to development of sporadic desmoid tumors as well as to the hereditary tumors that usually accompany familial adenomatous polyposis (FAP). Adenomatous polyposis coli (APC) mutations cause an intracellular accumulation of beta-catenin that results in abnormal signaling in the wnt/wi...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504013X13786659070316
更新日期:2013-01-01 00:00:00
abstract::The purpose of this study was to investigate the efficacy and safety of drug-eluting beads transarterial chemoembolization (DEB-TACE) treatment in Chinese hepatocellular carcinoma (HCC) patients and the prognostic factors for treatment response as well as survival. A total of 275 HCC patients were included in this pro...
journal_title:Oncology research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3727/096504019X15662966719585
更新日期:2020-02-07 00:00:00
abstract::Empty spiracles homeobox 2 (EMX2) is a homeodomain-containing transcription factor that plays an essential role in tumorigenesis. However, to the best of our knowledge, the role of EMX2 in human colorectal cancer (CRC) is still unclear. Thus, the aim of this study was to investigate the expression and role of EMX2 in ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14756640150695
更新日期:2017-04-14 00:00:00
abstract::Astragaloside IV (AS-IV) is an active ingredient in Astragalus membranaceus and is involved in various biological processes, such as regulating the immune system, and counteracting inflammation and malignancy. The aim of this study was to explore the effect of AS-IV on non-small cell lung cancer (NSCLC) cells. Cell co...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15344989701565
更新日期:2019-03-29 00:00:00
abstract::The potential of American ginseng (AG) ( Panax quinquefolium), a commonly used herbal remedy believed to have anticarcinogenic effects, to prevent the development of mammary tumors was evaluated in a mouse model of dimethylbenzanthracene (DMBA)-induced mammary carcinoma. Ginsenosides, believed to be the active compone...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/000000006783981017
更新日期:2006-01-01 00:00:00
abstract::Carbonic anhydrase (CA) IX has emerged as a promising target for cancer therapy. It is highly upregulated in hypoxic regions and mediates pH regulation critical for tumor cell survival as well as extracellular acidification of the tumor microenvironment, which promotes tumor aggressiveness via various mechanisms, such...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14965111926391
更新日期:2018-03-05 00:00:00
abstract::Cervical cancer is one of the most common neoplastic diseases affecting women, with a combined worldwide incidence of almost half a million new cases. Considering the fact that IL-18 plays an important role in the interactions among T cells, NK cells, and macrophages and induces IFN-gamma production, efforts should be...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504008785114156
更新日期:2008-01-01 00:00:00
abstract::Numerous studies have observed that workers in ferriferous industries have an elevated risk of respiratory tract neoplasia. Research at the cellular and animal model levels indicates that iron compounds, per se, are carcinogenic. However, some investigators have suggested that inhaled iron compounds are merely carrier...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::The mechanism of action of the anthraquinone antileukemic drug mitoxantrone (MIT) was investigated and compared with that of daunorubicin (DNR) with emphasis on the interaction with DNA to clarify the more potent cytotoxic activity of MIT than DNR in human leukemia cells. MIT showed similar characteristics to those of...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP/PBXIP1) is a nucleo-cytoplasmic shuttling protein, and its expression is associated with cancer aggressiveness. However, the role of HPIP in ovarian cancer is still unclear. Here, we aimed to clarify the role of HPIP in epithelial-m...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14575597858654
更新日期:2016-01-01 00:00:00
abstract::Chemoresistance is a major impediment to the successful treatment of cancer. It involves various mechanisms, including defects in the apoptosis program that is induced by anticancer drugs. To further explore the mechanisms underlying the development of chemoresistance in ovarian carcinoma after cisplatin (CDDP) treatm...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504003108748410
更新日期:2003-01-01 00:00:00
abstract::We have investigated the role of protein kinase C (PKC) in the multidrug resistance (MDR) phenotype of human KB carcinoma cell lines. The PKC activator 12-O-tetradecanoylphorbol-13-acetate (TPA) reduced daunomycin accumulation in both drug-sensitive KB-3-1 and MDR KB-C1 cells in a time-dependent manner. The inactive p...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Ovarian cancer is the most lethal disease among gynecological malignancies. More effective therapy is required to counter high recurrence rates and chemotherapy resistance. We investigated the efficacy and molecular mechanisms of three combined treatments (TCTs)-a novel histone deacetylase (HDAC) inhibitor OBP-801/YM7...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14850164661097
更新日期:2017-09-21 00:00:00
abstract::Concentrations of the flame retardant 2,2',4,4'-tetrabrominated diphenyl ether (2,2',4,4'-TeBDE) in the adipose tissue of 77 individuals from Sweden were determined. The subjects were recruited during the time period 1995-97 and encompassed both men and women ranging from 28 to 85 years in age. Of the subjects include...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The expression of survivin molecules has been confirmed in many types of cancer cells, including colon cancer cells, and they are considered important antiapoptotic molecules. Recent studies have revealed the existence of different splicing forms of survivin molecules; however, no studies have examined their expressio...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504010x12767359113848
更新日期:2010-01-01 00:00:00
abstract::Aberrant expression of microRNA-92a (miR-92a) has been investigated in various cancers. However, the function and mechanism of miR-92a in acute myeloid leukemia (AML) remain to be elucidated. Our data showed that miR-92a was evidently downregulated and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was remarkably ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14829256525028
更新日期:2017-08-07 00:00:00