Abstract:
:We previously reported that vasoactive intestinal polypeptide (VIP) significantly inhibited Matrigel invasion and haptotactic migration of murine colon 26-L5 carcinoma in vitro. To extend our study, we investigated the inhibitory mechanisms of VIP on Matrigel invasion of colon 26-L5 carcinoma, and the effect on metastatic properties of the tumor cells. VIP inhibited the invasion of the tumor cells in a concentration-dependent manner without affecting their growth, and achieved approximately 50% reduction at 10(-6) M. VIP also suppressed cell motility with a similar inhibition rate to the invasion assay. Time course study revealed that the motility was reduced by 40% when the tumor cells were preincubated with 10(-6) M VIP for 3 h. In contrast, 6-h pretreatment with 10(-6) M VIP caused the increased ability of the adhesion to both fibronectin and laminin with a 50% enhancement. A large amount of VIP1 receptor transcripts was expressed in the cells, whereas VIP2 receptor was undetectable, by RT-PCR and subsequent Southern blot hybridization. A specific antagonist for VIP1 receptor reversed the suppressed motility induced by VIP. Cryostat sections showed that the 3-h pretreatment of tumor cells with VIP caused the reduction of the arrest in the livers at 6 h after the tumor inoculation into a portal vein of mice. VIP could prevent the experimental liver metastasis of the tumor cells in a dose-dependent manner. The cells pretreated with 10(-6) M VIP for 3 h also showed the reduced ability of the liver metastasis. These results suggest that VIP could block the invasion and the metastasis of colon 26-L5 carcinoma through suppression of their motility.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Ogasawara M,Murata J,Ayukawa K,Saiki Isubject
Has Abstractpub_date
1998-01-01 00:00:00pages
361-70issue
7eissn
0965-0407issn
1555-3906journal_volume
10pub_type
杂志文章abstract::There is growing evidence on the clinical significance of Tumor Microenvironment (TME) cells in predicting prognosis and therapeutic effects. However, cell interactionsin tumor microenvironments have not been thoroughly studied or systematicallyanalyzed so far. In this study, 22 immune cell components in the lung aden...
journal_title:Oncology research
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