Abstract:
:HeLa cells were transfected with full-length multidrug resistance protein (MRP) cDNA and with MRP cDNAs that had been mutated at certain nucleotide binding domains. Stable transfectants were isolated and those producing equivalent amounts of P190 were tested in cytotoxicity assays using a variety of chemotherapeutic agents. The results demonstrate that deletions in the C-motif of NBD1 or the A-motif of NBD2 have a pronounced effect in reducing resistance levels to adriamycin, vincristine, or etoposide (VP-16). Single-site mutations of lysine in these same motifs reduce IC50 values but less than that observed with the deletion mutants. Additional studies have demonstrated an increase in drug accumulation and reduction in drug efflux in NBD deletion and single-site mutants. The results of this study therefore identify two lysines of the NBD A- and C-motifs that are critical for MRP-mediated multidrug resistance. The results also provide definitive evidence that resistance occurring as a result of MRP overexpression is related to enhanced levels of an ATP-dependent efflux pump.
journal_name
Oncol Resjournal_title
Oncology researchauthors
Zhu Q,Sun H,Center MSsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
229-36issue
5eissn
0965-0407issn
1555-3906journal_volume
9pub_type
杂志文章abstract::O6-Alkylguanine-DNA alkyltransferase (AGT) is a DNA repair protein that reverses alkylation damage produced by chloroethylnitrosoureas and is a major determinant of cellular resistance to adjuvant chemotherapy with these drugs. AGT activity was measured in 119 samples from 69 patients, including normal, tumor, and dis...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::In colorectal cancers, the local cytokine network and the levels of nitric oxide (NO) and reactive oxygen species (ROS) are known to be closely related to cancer progression and metastasis, but the influence of the currently administered therapies on the cancer microenvironment is not completely understood. We analyze...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504010x12671222663593
更新日期:2010-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are emerging as pivotal regulators in the development and progression of various cancers, including renal cell carcinoma (RCC). MicroRNA-384 (miR-384) has been found to be an important cancer-related miRNA in several types of cancers. However, the role of miR-384 in RCC remains unclear. In this stud...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15035025554553
更新日期:2018-04-10 00:00:00
abstract::Heme oxygenase-1 (HO-1) plays an important role in the progression of several malignancies including breast cancer. However, its role in breast cancer metastasis is still ambiguous. In this study, we observed the effect of HO-1 on mouse mammary carcinoma metastasis using the in vivo tumor metastasis model. Our results...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15415906335771
更新日期:2019-06-21 00:00:00
abstract::Accumulated studies have strongly implicated aberrantly expressed microRNAs (miRNAs) in carcinogenesis and cancer progression of various cancers, including colorectal cancer (CRC). Hence, a comprehensive study of miRNAs and their association with CRC may be a promising therapeutic method for patients with this maligna...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504018X15188747585738
更新日期:2018-10-17 00:00:00
abstract::The present study evaluated the modulatory effects of zinc on colonic membrane fluidity and surface abnormalities following 1,2 dimethylhydrazine (DMH)-induced colon carcinogenesis. Rats were segregated into four groups: normal control, DMH treated, zinc treated, DMH + zinc treated. Colon carcinogenesis was initiated ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504009789745665
更新日期:2009-01-01 00:00:00
abstract::Solid tumors involving glandular organs express mucin glycoprotein that is eventually shed into the circulation. As a result, these proteins can easily be measured in the serum and be used as potential tumor markers. The most commonly used tumor markers for breast cancer are CA27-29 and CA15-3, which both measure the ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15128550060375
更新日期:2018-04-10 00:00:00
abstract::Electron-affinic compounds with strong DNA intercalating properties have demonstrated less than the expected radiosensitization due to restriction of their mobility along the DNA backbone and their lower extravascular diffusion in tumors. A 2-nitroimidazole linked 1,2,3,4-tetrahydroacridine derivative (THNLA-1) has be...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::We previously reported that vasoactive intestinal polypeptide (VIP) significantly inhibited Matrigel invasion and haptotactic migration of murine colon 26-L5 carcinoma in vitro. To extend our study, we investigated the inhibitory mechanisms of VIP on Matrigel invasion of colon 26-L5 carcinoma, and the effect on metast...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Venous thromboembolism (VTE) occurs at an increased incidence in cancer patients. A cancer-related hypercoagulable state has been considered to play role in this phenomenon. Preclinical data suggest an association between tumor expression of MET proto-oncogene (MET) and a hypercoagulable state, resulting in VTE. We in...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504010x12828372551786
更新日期:2010-01-01 00:00:00
abstract::Transforming growth factor-β1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) may contribute to tumor metastasis. TGF-β1-induced EMT in H1975 cells (a human NSCLC cell line) resulted in the adoption of mesenchymal responses that were predominantly mediated via the TGF-β1-...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15051723858706
更新日期:2019-05-07 00:00:00
abstract::Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) are used as first-line agents for treating nonsquamous cell lung cancer with EGFR mutation, there are many patients who have to receive these drugs following platinum-based chemotherapy. This study was designed to define whether exposure ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504012x13285365944337
更新日期:2011-01-01 00:00:00
abstract::Thymosin-beta-4 (TB4) as an actin-sequestering peptide has been detected outside of cells in blood plasma or in wound fluid. TB4 induces tumor metastasis and paclitaxel resistance, which is the most significant obstacle to successful therapy in tumors. Here we investigated the inhibitory effect of TB4 peptides on tumo...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504007783438349
更新日期:2007-01-01 00:00:00
abstract::Chemoresistance is a major impediment to the successful treatment of cancer. It involves various mechanisms, including defects in the apoptosis program that is induced by anticancer drugs. To further explore the mechanisms underlying the development of chemoresistance in ovarian carcinoma after cisplatin (CDDP) treatm...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504003108748410
更新日期:2003-01-01 00:00:00
abstract::Neuroendocrine tumors (NETs) represent a relatively rare form of neoplasias for which, in advanced unresectable stages, palliative treatment options remain limited largely to the use of somatostatin analogues or chemotherapy. Several newer targeted therapeutic options, alone or in combination with somatostatin analogu...
journal_title:Oncology research
pub_type: 杂志文章,评审
doi:10.3727/096504011x13123323849799
更新日期:2011-01-01 00:00:00
abstract::Protein disulfide isomerases A6 (PDIA6) belongs to the PDI family. Recently, PDIA6 was found to have a close association with various cancers. However, there has been little investigation into the biological functions of PDIA6 in bladder cancer (BC). In this study, we explored the expression pattern and functional sig...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14761811155298
更新日期:2017-04-14 00:00:00
abstract::Small-cell lung cancer (SCLC) has a significantly worse prognosis than other forms of bronchogenic carcinoma. Because transforming growth factor beta (TGF-beta) appears to play an important role in the pathogenesis of SCLC, we examined the status of the TGF-beta receptor system in a series of 11 human small-cell carci...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::We observed a putative immunocytolytic factor secreted in the supernatant of several pancreatic adenocarcinoma cell lines that induces apoptosis in lymphocytes. A marked reduction in the viability of the target splenocytes was evident after coincubation with the supernatants from various pancreatic adenocarcinoma cell...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Accumulating evidence indicates that microRNA-205 (miR-205) is involved in tumor initiation, development, and metastasis in various cancers. However, its functions in neuroblastoma (NB) remain largely unclear. Here we found that miR-205 was significantly downregulated in human NB tissue samples and cell lines. miR-205...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X14974834436195
更新日期:2018-04-10 00:00:00
abstract::Epidermal growth factor receptor (EGFR) is commonly overexpressed in non-small cell lung cancer (NSCLC) and its tyrosine kinase phosphorylation is thought to be an ideal target in the treatment of patients with NSCLC. In the present study, we examined surgically obtained specimens from a series of 36 NSCLC patients fo...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504003108748348
更新日期:2003-01-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies, with a poor prognosis and high recurrence rate. In the present study, we identified CD133, one of the markers of cancer stem cells, as a novel molecular target of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). In four human HCC ce...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14685034103950
更新日期:2016-10-27 00:00:00
abstract::Expression of sphingosine kinase 1 (SPHK1) plays a role in colorectal cancer progression. This study aimed to demonstrate the mechanism of human colorectal cancer cell metastatic phenotype through SPHK1 knockdown. Human colorectal cancer HT-29 cells were stimulated by phorbol 12-myristate 13-acetate (PMA) with or with...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504012x13342463747450
更新日期:2011-01-01 00:00:00
abstract::Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504013X13786659070316
更新日期:2013-01-01 00:00:00
abstract::Agosterol A (AG-A) is a novel agent that reverses P-glycoprotein (P-gp) and multidrug resistance protein-1 (MRP1)-meditated multidrug resistance (MDR). We have synthesized [125I]11-azidophenyl agosterol A (azidoAG-A), a photoaffinity analog of AG-A, and characterized its binding to P-gp in membrane vesicles prepared f...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/000000003108748595
更新日期:2003-01-01 00:00:00
abstract::Tamoxifen causes a mitochondrial transmembrane potential dysfunction and ATP depletion, which may play a role in tamoxifen cytotoxicity. Administration of oligomycin-2 deoxy glucose (2DG) enhanced tamoxifen antiproliferative effects, which may be due to exacerbated ATP depletion following tamoxifen and oligomycin-2DG ...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504009x12596189659204
更新日期:2009-01-01 00:00:00
abstract::We applied a differential display method to screen mRNAs isolated from a newly established cell line that carried a wild-type p53 transgene under control of the lactose operon. To investigate the p53 signaling pathway, we looked for genes whose expression was significantly induced or suppressed by induction of wild-ty...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::It has been demonstrated that microRNAs (miRNAs) act as oncogenes or tumor suppressors in a variety of cancers. Our previous work suggested that miR-10a/b functioned as a tumor suppressor in gastric cancer, and miR-10b was also reported to be significantly downregulated in advanced stage cervical cancer tissues. Howev...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504016X14611963142173
更新日期:2016-01-01 00:00:00
abstract::The anticancer drug Adriamycin is widely used in cancer chemotherapy and is classified as a topoisomerase II inhibitor. However, in the presence of formaldehyde, Adriamycin also forms high levels of DNA adducts. In this study, a new series of butyric acid and formaldehyde-releasing drugs related to AN9 (pivaloyloxymet...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504005776382305
更新日期:2005-01-01 00:00:00
abstract::Dysregulation of SUMO-specific protease 1 (SENP1) expression has been reported in several kinds of cancer, including human colorectal and prostate cancers, proposing SENP1 as an oncogene with a critical role in cancer progression. miR-133a-3p has been reported as a tumor suppressor in several malignant neoplasias. How...
journal_title:Oncology research
pub_type: 杂志文章
doi:10.3727/096504017X15004613574679
更新日期:2018-06-11 00:00:00
abstract::Since recombinant human granulocyte colony-stimulating factor (rhG-CSF) recruits hematopoietic progenitor cells into the cell cycle, the possibility remains that simultaneous injection of rhG-CSF and chemotherapeutic agents may aggravate the chemotherapy-induced neutropenia by sensitizing normal progenitor cells to th...
journal_title:Oncology research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00