Abstract：
PURPOSE:Human embryonic stem cells (hESC) hold a great potential for regenerative medicine because, in principle, they can differentiate into any cell type found in the human body. In addition, studying the effect of ionizing radiation (IR) on hESC may provide valuable information about the response of human cells to IR exposure in their most naive state, as well as the consequences of IR exposure on the development of organisms. However, the effect of IR, in particular radionuclide uptake, on the pluripotency, proliferation and survival of hESC has not been extensively studied. METHODS:In this study we treated cultured hESC with 5-[(125)I]iodo-2'-deoxyuridine ((125)IdU), a precursor of DNA synthesis. Then we measured the expansion of colonies and expression of pluripotency markers in hESC. RESULTS:We found that uptake of (125)IdU was similar in both hESC and HT1080 human fibrosarcoma cells. However, treatment with 0.1 μCi/ml (125)IdU for 24 hours resulted in complete death of the hESC population; whereas HT1080 cancer cells continued to grow. Treatment with a 10-fold lower dose (125)IdU (0.01 μCi/ml) resulted in colonies of hESC becoming less defined with numerous cells growing in monolayer outside of the colonies showing signs of differentiation. Then we analyzed the expression of pluripotency markers (octamer-binding transcription factor 4 [Oct-4] and stage-specific embryonic antigen-4 [SSEA4]) in the surviving hESC. We found that hESC in the surviving colonies expressed pluripotency markers at levels comparable with those in the non-treated controls. CONCLUSIONS:Our results provide important initial insights into the sensitivity of hESC to IR, and especially that produced by the decay of an internalized radionuclide.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Panyutin IV,Eniafe R,Panyutin IG,Neumann RDdoi
10.3109/09553002.2012.700435subject
Has Abstractpub_date
2012-12-01 00:00:00pages
954-60issue
12eissn
0955-3002issn
1362-3095journal_volume
88pub_type
杂志文章abstract：:The relationship between ionizing radiation-induced cell killing and DNA damage measured by the micronucleus and apoptosis assays was determined in three established cell lines (L929, HL-60, and Chang). Irradiation experiments revealed a dose-dependent increase of micronucleated cells until a certain dose was reached....
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550371
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abstract：:The scission of pentadeoxythymidylic acid, d(pT)5, by monochromatic soft X-rays on (2153 eV) and below (2147 eV) the K-shell absorption peak of phosphorus was studied as a model of strand breakage in DNA. Samples dried on glass plates were irradiated by monochromatic soft X-rays in vacuo, and the products were analyse...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550201
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abstract：:The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
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更新日期：1996-04-01 00:00:00
abstract：:Purpose: This review discusses recent developments in our understanding of biological and physiological mechanisms underlying radiation cataractogenesis. The areas discussed include effects of low-dose exposures to the lens including potential relevance of non-targeted effects, the development of new personal-protecti...
journal_title：International journal of radiation biology
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abstract：:Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
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abstract：PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
journal_title：International journal of radiation biology
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abstract：:PACE (programmable, autonomously controlled electrodes) pulsed field gel electrophoresis technology was used to develop a DNA double-strand break assay that simultaneously combined (1) resolution across a broad range of DNA sizes, (2) high sensitivity (in terms of detecting dsb at low doses) and (3) speed. A 48 h PACE...
journal_title：International journal of radiation biology
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abstract：PURPOSE:To honour perhaps the most influential man in the radiation sciences, Henry S. Kaplan, by reviewing the field of tumour hypoxia, one of his many interests. RESULTS:It was postulated over 50 years ago by Thomlinson and Gray that human solid tumours would contain hypoxic cells and that they would exert a negativ...
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pub_type： 历史文章
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abstract：PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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更新日期：2013-04-01 00:00:00
abstract：:SR 2508 (etanidazole) (1 mM) or buthionine sulphoximine (BSO, 50 microM) or both drugs together did not radiosensitize oxic V79 Chinese hamster cells irradiated at either an acute dose rate (2.35 Gy/min) or at a low dose rate (0.117 Gy/min). BSO pretreatment (15 h at 37 degrees C) depleted cellular glutathione (GSH) t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550121
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abstract：PURPOSE:Static magnetic field (SMF) exposure was shown experimentally to beneficially affect the healing process. The aim of this randomized, controlled trial was to evaluate the pain-inhibitory effect of local exposure to SMF on temporomandibular disorders, on alveolitis and on aphta in the oral mucosa. MATERIALS AND...
journal_title：International journal of radiation biology
pub_type： 杂志文章,随机对照试验
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abstract：PURPOSE:To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. MATERIALS AND METHODS:Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasin...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000410001723866
更新日期：2004-07-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550211
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551821
更新日期：1993-11-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143987
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550361
更新日期：1991-02-01 00:00:00
abstract：:Induction of mutations to 6-thioguanine resistance (TGr) by gamma-rays at three different dose-rates and molecular changes in the HPRT gene were studied in human lymphoblastoid WIL2-NS cells. Mutant induction showed a curvilinear dose-response for acute irradiation (30 Gy/h). The total mutant frequency was lower after...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145201
更新日期：1996-08-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500303690
更新日期：2005-08-01 00:00:00
abstract：:I calculate the probability that a single high-energy ionizing particle or photon makes two widely spaced double-strand breaks in the same DNA molecule. Deletions (or inversions) between two breaks formed by the same incident particle are linear in radiation dose and occur even at extremely low dose-rates; deletions b...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144699
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND:Global warming directly influencing ozone layer depletion, which eventually is increasing ultraviolet radiation penetration having far-reaching impacts on living biota. This particularly influences the primary producer microalgae which are the basic unit of food webs in the aquatic habitats. Therefore, it is...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1851060
更新日期：2020-12-03 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.545861
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.530337
更新日期：2011-03-01 00:00:00
abstract：:In mice, external X- or gamma-irradiation may induce thymic lymphomas or myeloid leukaemias, while bone-seeking alpha-emitters may induce osteosarcomas and, to a lesser extent, acute myeloid leukaemia. The present paper aims to review briefly some of the experimental data with respect to the molecular mechanisms under...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014550851
更新日期：1990-04-01 00:00:00
