Enhancement of SR 2508 (etanidazole) radiosensitization by buthionine sulphoximine at low-dose-rate irradiation.


:SR 2508 (etanidazole) (1 mM) or buthionine sulphoximine (BSO, 50 microM) or both drugs together did not radiosensitize oxic V79 Chinese hamster cells irradiated at either an acute dose rate (2.35 Gy/min) or at a low dose rate (0.117 Gy/min). BSO pretreatment (15 h at 37 degrees C) depleted cellular glutathione (GSH) to less than or equal to 1% of control level and radiosensitized hypoxic cells at both dose rates with an enhancement ratio (ER) of 1.2. SR 2508 alone radiosensitized hypoxic cells equally at both dose rates with an ER of 1.5. However, ER values of 2.2 and 2.5 were obtained with 1 mM SR 2508 in GSH-depleted cells at acute and low dose rate, respectively, with no significant difference between the two, i.e. there is no dose rate dependence for this potentiation. Since BSO increases SR 2508 radiosensitization and the combined BSO + SR 2508 treatment is extremely cytotoxic to hypoxic cells, our results suggest that combining BSO with SR 2508 will be useful in brachytherapy as well as external-beam therapy if the toxicity from both drugs in vivo is less than the gain in radiosensitization achieved.


Int J Radiat Biol


Wong RS,Basas RA,Ling CC




Has Abstract


1991-01-01 00:00:00














  • Relationship between radiation response and p53 status in human bladder cancer cells.

    abstract::Mutations in the p53 tumour suppressor gene are found at high frequency in bladder cancer. There is strong evidence that p53 plays an important role in controlling the cell cycle after DNA damage by ionizing radiation. However, the effect of loss of p53 function on radiosensitivity is not yet clear. Radiotherapy combi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ribeiro JC,Barnetson AR,Fisher RJ,Mameghan H,Russell PJ

    更新日期:1997-07-01 00:00:00

  • Radiation metabolomics in the quest of cardiotoxicity biomarkers: the review.

    abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Gramatyka M,Sokół M

    更新日期:2020-03-01 00:00:00

  • IkappaBalpha degradation is not a requirement for the X-ray-induced activation of nuclear factor kappaB in normal rat astrocytes and human brain tumour cells.

    abstract:PURPOSE:To investigate the mechanism of NFkappaB activation by X-rays in normal primary rat astrocytes. MATERIALS AND METHODS:Primary cultures of type I astrocytes generated from the cortex of neonatal rats were exposed to X-rays with and without various kinase inhibitors and a protease inhibitor. The nuclear or cytop...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Raju U,Gumin GJ,Noel F,Tofilon PJ

    更新日期:1998-11-01 00:00:00

  • Increased mutability and decreased repairability of a three-lesion clustered DNA-damaged site comprised of an AP site and bi-stranded 8-oxoG lesions.

    abstract:PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of c...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cunniffe S,Walker A,Stabler R,O'Neill P,Lomax ME

    更新日期:2014-06-01 00:00:00

  • Protein gamma-radiolysis in frozen solutions is a macromolecular surface phenomenon: fragmentation of lysozyme, citrate synthase and alpha-lactalbumin in native or denatured states.

    abstract:PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alp...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Audette M,Chen X,Houée-Levin C,Potier M,Le Maire M

    更新日期:2000-05-01 00:00:00

  • Effect of GSM-900 and -1800 signals on the skin of hairless rats. II: 12-week chronic exposures.

    abstract:PURPOSE:The purpose of this work was to determine whether the cellular components of Hairless-rat skin are affected by a chronic local exposure to non-ionizing radiations of Global Mobile Phone System: GSM-900 or -1800 radiations at specific absorption rate (SAR) 2.5 and 5 W/kg. MATERIALS AND METHODS:A selected part o...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sanchez S,Masuda H,Billaudel B,Haro E,Anane R,Lévêque P,Ruffie G,Lagroye I,Veyret B

    更新日期:2006-09-01 00:00:00

  • Delayed effects of prenatal low-dose irradiation in the white matter of the rat brain.

    abstract:PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Reyners H,Gianfelici de Reyners E,Yan J,De Saint-Georges L,Desaintes C

    更新日期:1999-10-01 00:00:00

  • Therapy for prevention and treatment of skin ionizing radiation damage: a review.

    abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Soriano JL,Calpena AC,Souto EB,Clares B

    更新日期:2019-05-01 00:00:00

  • Mechanisms underlying the radioprotective effect of histamine on small intestine.

    abstract:PURPOSE:To examine the protective effects of histamine on intestinal damage produced by gamma-radiation. MATERIALS AND METHODS:56 mice were divided into 4 groups. Histamine and Histamine-10 Gy groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 20 hours before irradiation and continued until ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Medina VA,Croci M,Mohamad NA,Massari N,Garbarino G,Cricco GP,Núñez MA,Martín GA,Crescenti EJ,Bergoc RM,Rivera ES

    更新日期:2007-10-01 00:00:00

  • Fibroblasts from ataxia telangiectasia (AT) and AT heterozygotes show an enhanced level of residual DNA double-strand breaks after low dose-rate gamma-irradiation as assayed by pulsed field gel electrophoresis.

    abstract::Skin fibroblasts from ataxia telangiectasia (AT) patients, obligate AT heterozygotes (ATH) and normal individuals were studied for colony-forming ability and repair of DNA double-strand breaks (dsb) after gamma-irradiation. AT cells were three to four times more radiosensitive than normal cells at high and low dose-ra...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Blöcher D,Sigut D,Hannan MA

    更新日期:1991-11-01 00:00:00

  • The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation.

    abstract:PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU5593...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cmielova J,Havelek R,Kohlerova R,Soukup T,Bruckova L,Suchanek J,Vavrova J,Mokry J,Rezacova M

    更新日期:2013-07-01 00:00:00

  • The shape of DNA elution dose-response curves under non-denaturing conditions: the contribution of the degree of chromatin condensation.

    abstract::We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Okayasu R,Iliakis G

    更新日期:1992-04-01 00:00:00

  • Effects of 36.6 GHz and static magnetic field on degree of endoreduplication in Drosophila melanogaster polytene chromosomes.

    abstract::Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dyka LD,Shakina LA,Strashnyuk VY,Shckorbatov YG

    更新日期:2016-01-01 00:00:00

  • Characterization of radiation-induced damage in d(TpApCpG).

    abstract::The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modific...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Paul CR,Budzinski EE,Maccubbin A,Wallace JC,Box HC

    更新日期:1990-11-01 00:00:00

  • Evaluation of continuous low dose rate versus acute single high dose rate radiation combined with oncolytic viral therapy for prostate cancer.

    abstract:PURPOSE:Conditionally Replicative Adenovirus (CRAd) has been previously demonstrated to augment the activity of radiation, resulting in synergy of cell kill. However, previous models combining radiation with CRAd have not focused on the methods of radiation delivery. MATERIALS AND METHODS:We model the combination of a...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Liu C,Zhang Y,Liu MM,Zhou H,Chowdhury W,Lupold SE,Deweese TL,Rodriguez R

    更新日期:2010-03-01 00:00:00

  • Mechanism of protection against radiation-induced DNA damage in plasmid pBR322 by caffeine.

    abstract:PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Kumar SS,Devasagayam TP,Jayashree B,Kesavan PC

    更新日期:2001-05-01 00:00:00

  • Low dose radiation-induced endothelial cell retraction.

    abstract::We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characteriza...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Kantak SS,Diglio CA,Onoda JM

    更新日期:1993-09-01 00:00:00

  • Lambda-prophage induction in repair-deficient and wild type E. coli strains by gamma-rays and heavy ions.

    abstract::Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Bonev MN,Kozubek S,Krasavin EA,Amirtajev KG

    更新日期:1990-05-01 00:00:00

  • Different radiosensitivity of CD4(+)CD25(+) regulatory T cells and effector T cells to low dose gamma irradiation in vitro.

    abstract:PURPOSE:To determine the radiosensitivity difference of human Cluster of Differentiation (CD)4(+)CD25(+) regulatory T cells (Treg) and effector T cells to low dose gamma ray and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS:Blood samples were collected from five health subjects and five patients ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cao M,Cabrera R,Xu Y,Liu C,Nelson D

    更新日期:2011-01-01 00:00:00

  • DNA radiolysis in DNA-protein complexes: a stochastic simulation of attack by hydroxyl radicals.

    abstract:PURPOSE:To propose an improved version of RADACK, a stochastic simulation of radiolytic attack on DNA, that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. To apply it to the natural lactose operator-repressor complex taking advantage of recently reported str...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Begusova M,Giliberto S,Gras J,Sy D,Charlier M,Spotheim-Maurizot M

    更新日期:2003-06-01 00:00:00

  • Non-linear dependence of the dielectric properties of chick embryo myoblast membranes exposed to a sinusoidal 50 Hz magnetic field.

    abstract::Primary chick embryo myoblasts can be a useful tool for studying the developmental events which accompany myoblast differentiation, particularly myoblast membrane fusion. To determine whether the electrical properties and/or fusion in these systems are affected by 50 Hz magnetic fields, chick embryo myoblast cultures ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Grandolfo M,Santini MT,Vecchia P,Bonincontro A,Cametti C,Indovina PL

    更新日期:1991-12-01 00:00:00

  • Radiation-induced genomic instability: are epigenetic mechanisms the missing link?

    abstract:PURPOSE:This review examines the evidence for the hypothesis that epigenetics are involved in the initiation and perpetuation of radiation-induced genomic instability (RIGI). CONCLUSION:In addition to the extensively studied targeted effects of radiation, it is now apparent that non-targeted delayed effects such as RI...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Aypar U,Morgan WF,Baulch JE

    更新日期:2011-02-01 00:00:00

  • Repair of DNA strand breaks at hyperthermic temperatures in Chinese hamster ovary cells.

    abstract::The repair of DNA double-strand breaks was measured by pH 7.2 filter elution in cells incubated at 25-45 degrees C either before or after X-irradiation. Exposure to 45 degrees C for 15 minutes immediately prior to X-irradiation significantly increased both the half-time for DNA double-strand break closure and the numb...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Warters RL,Axtell J

    更新日期:1992-01-01 00:00:00

  • Repair of dGMP hydroxyl radical adducts by verbascoside via electron transfer: a pulse radiolysis study.

    abstract::The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Li W,Zheng R,Su B,Jia Z,Li H,Jiang Y,Yao S,Lin N

    更新日期:1996-04-01 00:00:00

  • Transformation-sensitive cells in G2/M-phase are not promoted by TPA following 137Cs gamma-rays.

    abstract::Mouse C3H 10T1/2 cells are most sensitive to radiation-induced neoplastic transformation in the G2/M-phase of the cell cycle. When synchronized 10T1/2 cells were exposed to phorbol 12-myristate 13-acetate (TPA) after irradiation, transformation of cells not in the transformation-sensitive window was enhanced, but tran...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Wells RL,Cao J,Xing Y,He L,Elkind MM

    更新日期:1993-12-01 00:00:00

  • Modification of murine adult haemopoiesis and response to methyl nitrosourea following exposure to radiation at different developmental stages.

    abstract:PURPOSE:To investigate the hypothesis that the developmental phase at which an individual encounters radiation damage affects its long-term sensitivity to a subsequent tumourigenic insult. MATERIALS AND METHODS:Either the pregnant C57B16 mouse was exposed to 137Cs gamma-rays at 4 or 15 days post-conception (embryonic ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Hoyes KP,Hendry JH,Lord BI

    更新日期:2000-01-01 00:00:00

  • Lupeol enhances radiosensitivity of human hepatocellular carcinoma cell line SMMC-7721 in vitro and in vivo.

    abstract:PURPOSE:To investigate the effect of lupeol, a pentacyclictriterpene, on the radiosensitivity of a human hepatocellular carcinoma (HCC) in vitro and in vivo xenografts. METHODS:SMMC-7721 cells were exposed to γ-radiation with or without lupeol and assayed for proliferation, clonogenic survival, apoptosis and cell cycl...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Jin Y,Lyu Y,Tang X,Zhang Y,Chen J,Zheng D,Liang Y

    更新日期:2015-02-01 00:00:00

  • Putative determinants of the cellular response to hyperthermia.

    abstract::Recently, it has been demonstrated that two different thermal resistant states found in Chinese hamster cells, one transient, associated with thermotolerance, and the other permanent, associated with the increased expression of the cognate member of the hsp 70 family, are characterized by faster recovery from heat-ind...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Laszlo A,Davidson T,Hu A,Landry J,Bedford J

    更新日期:1993-05-01 00:00:00

  • Expression of genes and pathways associated with the B7-CD28 superfamily in response to irradiation of blood cells using 137Cs.

    abstract:PURPOSE:DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this st...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Silveira DA,Ribeiro FM,Simão ÉM,Mattos VLD,Góes EG

    更新日期:2020-12-17 00:00:00

  • Gamma and pulse radiolysis investigation of the reaction of desferrioxamine with superoxide anions.

    abstract::The kinetic scheme of the reaction of desferrioxamine (DFO) with O2-. was studied using pulse and gamma-radiolysis. The rate constant k(O2-. + DFO) is equal to 1.3 +/- 0.1 x 10(6) dm3 mol-1s-1 at pH 7.4. Studying the competition between DFO and ferricytochrome-c for O2-. generated by gamma-radiolysis, we observed that...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sabourault D,Ribiere C,Nordmann R,Houee-Levin C,Ferradini C

    更新日期:1989-12-01 00:00:00