Abstract：
:The scission of pentadeoxythymidylic acid, d(pT)5, by monochromatic soft X-rays on (2153 eV) and below (2147 eV) the K-shell absorption peak of phosphorus was studied as a model of strand breakage in DNA. Samples dried on glass plates were irradiated by monochromatic soft X-rays in vacuo, and the products were analysed by HPLC. The main products, in ascending order of the retention time, were thymine, 5'-dTMP, d(pT)2, d(pT)3, d(pT)4, d(pTp) and three unknown products (UK 1, 2 and 3), which were presumed to be d(pTpTp), d(pTpTpTp) and d(pTpTpTpTp), respectively. No difference between 2147 and 2153 eV irradiation in the nature of the induced products was detected, indicating that the K-shell absorption of phosphorus and the following Auger process produced the same types of products as those produced by photoabsorption at the other shells of phosphorus and at other atoms. The cross-sections for the induction of products at 2153 eV were 3.3-4.0 times larger than those at 2147 eV, the ratios of these values being scattered around the ratio (3.65) of absorbance of the sample between 2153 and 2147 eV. The dependence on the X-ray energy, however, almost disappeared after conversion from exposure to absorbed dose; the ratios of the G-values (number of products per 100 eV) of the products were 0.92-1.11. Photoabsorption at the K-shell of phosphorus induced products comparable or slightly less effectively than photoabsorption at the others. These results indicate that the K-shell absorption of phosphorus and the following Auger process do not have any characteristic effect on strand breakage in dry DNA, either qualitatively or quantitatively.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Yamada H,Kobayashi K,Hieda Kdoi
10.1080/09553009314550201subject
Has Abstractpub_date
1993-02-01 00:00:00pages
151-9issue
2eissn
0955-3002issn
1362-3095pii
V4ACKDPK8XPA6HD6journal_volume
63pub_type
杂志文章abstract：PURPOSE:To reduce retention and toxicity of the alpha particle emitter polonium-210 in rats by newly developed chelating agents. MATERIALS AND METHODS:Repeated subcutaneous chelation was conducted after intravenous injection of 210Po nitrate. For reduction of 210Po retention the treatment with vicinal dithiols meso-an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050151691
更新日期：2000-10-01 00:00:00
abstract：:Primary chick embryo myoblasts can be a useful tool for studying the developmental events which accompany myoblast differentiation, particularly myoblast membrane fusion. To determine whether the electrical properties and/or fusion in these systems are affected by 50 Hz magnetic fields, chick embryo myoblast cultures ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.545861
更新日期：2011-07-01 00:00:00
abstract：:Murine bone marrow contains osteogenic precursor cells that undergo differentiation during in vitro cultivation. In vitro these cells are potential target cells for alpha-irradiation-induced bone tumour formation. Under defined tissue culture conditions these differentiating cells were directly exposed to alpha-partic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551111
更新日期：1994-08-01 00:00:00
abstract：PURPOSE:Cells derived from individuals in which the ataxia telangiectasia (ATM) gene is mutated are hypersensitive to ionizing radiation. Whether differences in ATM protein levels exist among human malignant glioma cell lines and whether such differences are correlated with cellular radiosensitivity were determined. M...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141591
更新日期：1998-08-01 00:00:00
abstract：PURPOSE:To investigate the action of ultra-low dose beta-radiation (ULDBR) on isolated segments of blood vessels. MATERIALS AND METHODS:We used the pharmacological model of isolated rabbit carotid arteries with intact or mechanically removed endothelium. Specific vascular responses to beta-irradiation were registered ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000600562229
更新日期：2005-11-01 00:00:00
abstract：PURPOSE:The need to determine a reliable relative biological effectiveness (RBE) value for alpha exposures has become important as reports remain controversial. Although a radiation-weighting factor of 20 has been designated for alpha particles, uncertainty exists on realistic value of the RBE of alpha radiation. The a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1047986
更新日期：2015-08-01 00:00:00
abstract：:A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2018.1454616
更新日期：2019-01-01 00:00:00
abstract：:High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914551621
更新日期：1989-10-01 00:00:00
abstract：:We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characteriza...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551471
更新日期：1993-09-01 00:00:00
abstract：PURPOSE:DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this st...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1857454
更新日期：2020-12-17 00:00:00
abstract：PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or witho...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134528
更新日期：2000-09-01 00:00:00
abstract：PURPOSE:The present study was designed to reveal, whether the 30 min daily full body exposure of mice to an inhomogeneous static magnetic field (SMF) has a statistically significant effect on diabetic neuropathy. MATERIALS AND METHODS:Three different doses (100, 150, or 200 mg/kg) of streptozotocin (STZ) were administ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.518200
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802499212
更新日期：2008-11-01 00:00:00
abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：:Purpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1625462
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:Caveolin-1 is a membrane protein highly expressed in many tumors and plays an important role in tumor progression and metastasis. This review describes the structure of the Caveolin-1 protein and its pre-clinical and clinical significance, demonstrating that Caveolin-1 is a novel biomarker for radioresistance w...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2016.1222096
更新日期：2016-12-01 00:00:00
abstract：PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/095530099139845
更新日期：1999-07-01 00:00:00
abstract：PURPOSE:To compare colony-forming and comet assays on fibroblasts and lymphocytes of 32 breast cancer patients irradiated after breast-conserving operations and to correlate the results with acute clinical radiation reactions in the skin. MATERIAL AND METHODS:Skin fibroblasts were isolated and cultivated before radiot...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000210126466
更新日期：2002-07-01 00:00:00
abstract：PURPOSE:To evaluate the effects of 50 Hz magnetic fields (MF) on the development of cancer induced by ionizing radiation. MATERIALS AND METHODS:A total of 150 female CBA/S mice were randomized into three equal groups at the age of 3-5 weeks. One of the groups served as a 'cage-control group'. The two other groups were...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000010024696
更新日期：2001-04-01 00:00:00
abstract：PURPOSE:To investigate and compare the propensity of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL) obtained from unaffected healthy individuals and ataxia telangectasia (A-T) patients to undergo apoptosis after X-ray exposure. MATERIAL AND METHODS:The LCL were exposed to 1-4 Gy X-rays at a dose-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140041
更新日期：1999-06-01 00:00:00
abstract：PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alp...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138349
更新日期：2000-05-01 00:00:00
abstract：PURPOSE:The effects of radiation +/- hypogravity on immunologic function were investigated using the Partial Weight Suspension (PWS) model ( Wagner et al. 2010 ). MATERIALS AND METHODS:Mice were exposed to 0.5, 1, or 2 Gray (Gy) dose of gamma radiation and then placed in the PWS system for 4, 24, 48 hours, or 4 days. ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.595875
更新日期：2011-10-01 00:00:00
abstract：:Recent evidence indicates that isoeffect doses for spinal cord and kidney may be overestimated for fraction sizes as small as 1 or 2 Gy when calculated from a linear-quadratic (LQ) model fitted to data obtained for fraction sizes larger than 2 Gy. Reasons for this are unknown, but possible interpretations include exha...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551461
更新日期：1988-07-01 00:00:00
abstract：:Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50 degrees C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500156577
更新日期：2005-06-01 00:00:00
abstract：PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000902836404
更新日期：2009-05-01 00:00:00
abstract：PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050111541
更新日期：2000-08-01 00:00:00
abstract：:The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550601
更新日期：1994-05-01 00:00:00
abstract：PURPOSE:Following ionizing radiation exposure, intestinal crypt regeneration is possible but it is still not known if regenerating crypts give rise to differentiated functional epithelial cells on villi. The aim of this study was to demonstrate that irradiated progeny of enterocytic precursor cells are capable of proli...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500091923
更新日期：2005-03-01 00:00:00
abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.518204
更新日期：2011-02-01 00:00:00
