Strategy and tactics of disarming GHG at the source: N2O reductase crops.

abstract：:The mapping of the human genome was completed earlier this year and efforts are underway to understand the role of gene products (i.e. proteins) in biological pathways and human disease and to exploit their functional roles to derive protein therapeutics and protein-based drugs. A key component to the next revolution ...

journal_title：Trends in biotechnology

authors： Maggio ET,Ramnarayan K

更新日期：2001-07-01 00:00:00

abstract：:Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and...

journal_title：Trends in biotechnology

authors： Even MS,Sandusky CB,Barnard ND

更新日期：2006-03-01 00:00:00

abstract：:Carotenoids are antioxidants with considerable pharmaceutical potential. More than 600 carotenoid structures are known but their availability is limited owing to practical difficulties associated with chemical synthesis and isolation from microorganisms or plant tissue. To overcome some of these problems, heterologous...

journal_title：Trends in biotechnology

authors： Sandmann G,Albrecht M,Schnurr G,Knörzer O,Böger P

更新日期：1999-06-01 00:00:00

abstract：:Flow cytometry is a rapid method for the quantitative measurement of light scattering and fluorescent properties of cells. Although this technique has been widely applied to biomedical and environmental studies, its potential as a tool in ecotoxicological studies has not yet been fully exploited. This article describe...

journal_title：Trends in biotechnology

authors： Stauber JL,Franklin NM,Adams MS

更新日期：2002-04-01 00:00:00

abstract：:The realization of the important biomedical roles of polyunsaturated fatty acids has led to the development of methods for obtaining and manipulating polyunsaturated lipids. Enzyme-mediated reactions have demonstrated unique advantages over chemical approaches and commercial lipase- and phospholipase-catalysed process...

journal_title：Trends in biotechnology

authors： Gill I,Valivety R

更新日期：1997-11-01 00:00:00

abstract：:A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...

journal_title：Trends in biotechnology

authors： Dan N

更新日期：2000-09-01 00:00:00

abstract：:The field of regenerative medicine has blossomed in recent decades. However, the ultimate goal of tissue regeneration - replacing damaged or aged cells with healthy functioning cells - still faces a number of challenges. In particular, better understanding of the role of the cellular niche in shaping stem cell phenoty...

journal_title：Trends in biotechnology

authors： Del Sol A,Okawa S,Ravichandran S

更新日期：2019-07-01 00:00:00

abstract：:Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...

journal_title：Trends in biotechnology

authors： Riedl DH,Dunn MK

更新日期：2013-10-01 00:00:00

abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...

journal_title：Trends in biotechnology

authors： Ponder BA

更新日期：1990-04-01 00:00:00

abstract：:Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...

journal_title：Trends in biotechnology

authors： Enriquez J

更新日期：2001-04-01 00:00:00

abstract：:Microbial contamination of polymer surfaces has become a significant challenge in domestic, industrial, and biomedical applications. Recent progress in our understanding of how topographical features of different length scales can be used to effectively and selectively control the attachment and proliferation of diffe...

journal_title：Trends in biotechnology

authors： Nguyen DHK,Bazaka O,Bazaka K,Crawford RJ,Ivanova EP

更新日期：2020-05-01 00:00:00

abstract：:Continued progress in cell-phone devices has made them powerful mobile computers, equipped with sophisticated, permanent physical sensors embedded as the default configuration. By contrast, the incorporation of permanent biosensors in cell-phone units has been prevented by the multivocal nature of the stimuli and the ...

journal_title：Trends in biotechnology

authors： Preechaburana P,Suska A,Filippini D

更新日期：2014-07-01 00:00:00

abstract：:Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...

journal_title：Trends in biotechnology

authors： Loi P,Galli C,Ptak G

更新日期：2007-05-01 00:00:00

abstract：:Process analytical technology (PAT) for the manufacture of monoclonal antibodies (mAbs) is defined by an integrated set of advanced and automated methods that analyze the compositions and biophysical properties of cell culture fluids, cell-free product streams, and biotherapeutic molecules that are ultimately formulat...

journal_title：Trends in biotechnology

authors： Maruthamuthu MK,Rudge SR,Ardekani AM,Ladisch MR,Verma MS

更新日期：2020-10-01 00:00:00

abstract：:Enantiomerically pure cyanohydrins are important synthetic intermediates for pharmaceuticals and agrochemicals. They are produced by enzyme-catalysed synthesis using oxynitrilases. Sufficient quantities of enzyme are available via cheap natural sources and there have been recent advances in overexpression production o...

journal_title：Trends in biotechnology

authors： Griengl H,Schwab H,Fechter M

更新日期：2000-06-01 00:00:00

abstract：:Protein engineers traditionally rely on amino acid substitutions to alter the functional properties of biomacromolecules, yet have largely overlooked the potential benefits of reorganizing the polypeptide chain of a protein by circular permutation (CP). By connecting the native protein termini via a covalent linker an...

journal_title：Trends in biotechnology

authors： Yu Y,Lutz S

更新日期：2011-01-01 00:00:00

abstract：:Vibrational spectroscopy using both infrared and Raman spectroscopies has been used in recent years with the aim to aid clinicians in disease diagnosis. More recently, these techniques have been applied to study stem cell differentiation and to determine stem cell presence in tissues. These studies have demonstrated t...

journal_title：Trends in biotechnology

authors： Sulé-Suso J,Forsyth NR,Untereiner V,Sockalingum GD

更新日期：2014-05-01 00:00:00

abstract：:Molecular imaging permits non-invasive visualization and measurement of molecular and cell biology in living subjects, thereby complementing conventional anatomical imaging. Herein, we review the emerging application of molecular imaging for the study of musculoskeletal biology. Utilizing mainly bioluminescence and fl...

journal_title：Trends in biotechnology

authors： Reumann MK,Weiser MC,Mayer-Kuckuk P

更新日期：2010-02-01 00:00:00

abstract：:Techniques to enable direct communication between the brain and computers/machines, such as the brain-computer interface (BCI) or the brain-machine interface (BMI), are gaining momentum in the neuroscientific realm, with potential applications ranging from medicine to general consumer electronics. Noninvasive BCI tech...

journal_title：Trends in biotechnology

authors： Min BK,Marzelli MJ,Yoo SS

更新日期：2010-11-01 00:00:00

abstract：:Gene expression noise is a significant source of phenotypic heterogeneity in otherwise identical populations of cells. Phenotypic heterogeneity can cause reversible drug resistance in diseased cells, and thus a better understanding of its origins might improve treatment strategies. In eukaryotes, data strongly suggest...

journal_title：Trends in biotechnology

authors： Miller-Jensen K,Dey SS,Schaffer DV,Arkin AP

更新日期：2011-10-01 00:00:00

abstract：:A major impact of genome projects on human health will be their contribution to the understanding of protein function. Proteins are the engines of biological systems, nearly all pharmaceuticals act on proteins and increasingly proteins themselves are used therapeutically. As biology enters the post-genomic era, resear...

journal_title：Trends in biotechnology

authors： Braun P,LaBaer J

更新日期：2003-09-01 00:00:00

abstract：:Many of the demands in the production of microalgae at a technical scale cannot presently be met by state-of-the-art cultivation technologies based on suspensions. Immobilized cultivation using porous substrate bioreactors (PSBRs) is characterized by a reduction of liquid reaction volumes by several orders of magnitud...

journal_title：Trends in biotechnology

authors： Podola B,Li T,Melkonian M

更新日期：2017-02-01 00:00:00

abstract：:Many pathogens have the ability to vary the antigenic composition of surface-associated antigens. Often, this variation is mediated by the regulation of gene expression. By varying its antigenicity, the pathogen is able to avoid host immune responses more efficiently; however, this makes the design of vaccines against...

journal_title：Trends in biotechnology

authors： Maskell D,Frankel G,Dougan G

更新日期：1993-12-01 00:00:00

abstract：:Photodynamic therapy (PDT) involves the use of a class of molecules that are activated by light, and which accumulate in, and are retained by, certain abnormal tissues. When these molecules (known as photosensitizers) are activated by light, they cause the formation of active forms of oxygen, which result in the killi...

journal_title：Trends in biotechnology

authors： Levy JG

更新日期：1995-01-01 00:00:00

abstract：:The therapeutic and commercial success of monoclonal antibodies (mAbs) has inspired innovative approaches aimed at increasing their potency and broadening their applicability. Among these, cocktails of recombinant human mAbs are a logical next step because they combine the technological advances made in the field of a...

journal_title：Trends in biotechnology

authors： Logtenberg T

更新日期：2007-09-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has developed into a huge threat to global health, and reducing it is an urgent priority for public health authorities. The importance of a healthy and balanced gut microbiome has been identified as a key protective factor against AMR development, but this can be significantly affected b...

journal_title：Trends in biotechnology

authors： Kelly SA,Rodgers AM,O'Brien SC,Donnelly RF,Gilmore BF

更新日期：2020-04-01 00:00:00

abstract：:Metabolomics embraces several strategies that aim to quantify cell metabolites in order to increase our understanding of how metabolite levels and interactions influence phenotypes. Metabolic footprinting represents a niche within metabolomics, because it focuses on the analysis of extracellular metabolites. Although ...

journal_title：Trends in biotechnology

authors： Mapelli V,Olsson L,Nielsen J

更新日期：2008-09-01 00:00:00

abstract：:The demands on drug discovery organizations have increased dramatically in recent years, partly because of the need to identify novel targets that are both relevant to disease and chemically tractable. This is leading to an industrial approach to traditional biology and chemistry, inspired in part by the revolution in...

journal_title：Trends in biotechnology

authors： Dean PM,Zanders ED,Bailey DS

更新日期：2001-08-01 00:00:00

abstract：:In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a ...

journal_title：Trends in biotechnology

authors： Clackson T,Wells JA

更新日期：1994-05-01 00:00:00

abstract：:Tremendous advances in our understanding of the human immunodeficiency viruses (HIVs) have been made through the use of animal models. However, there are limitations inherent in many of the current models that use either nonhuman primates, or viruses other than HIV-1. Researchers continue to search for improved models...

journal_title：Trends in biotechnology

authors： Lewis AD,Johnson PR

更新日期：1995-04-01 00:00:00