Abstract:
:Resorbable ceramics can promote the bony integration of implants. Their rate of degradation should ideally be synchronized with bone regeneration. This study examined the effect of rapidly resorbable calcium phosphate ceramics 602020, GB14, 305020 on adherence, proliferation and morphology of human bone-derived cells (HBDC) in comparison to β-TCP. The in vitro cytotoxicity was determined by the microculture tetrazolium (MTT) assay. HBDC were grown on the materials for 3, 7, 11, 15 and 19 days and counted. Cell morphology, cell attachment, cell spreading and the cytoskeletal organization of HBDC cultivated on the substrates were investigated using laser scanning microscopy and environmental scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells with strong focal contacts was verified. HBDC showed numerous well defined stress fibres which give proof of well spread and strongly anchored cells. Porous surfaces encouraged the attachment and spreading of HBDC. Further investigations regarding long term biomaterial/cell interactions in vitro and in vivo are required to confirm the utility of the new biomaterials.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Radetzki F,Wohlrab D,Zeh A,Delank KS,Mendel T,Berger G,Syrowatka F,Mayr O,Bernstein Adoi
10.3233/BME-2012-0678subject
Has Abstractpub_date
2011-01-01 00:00:00pages
307-21issue
5-6eissn
0959-2989issn
1878-3619pii
E2690218M58NW255journal_volume
21pub_type
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