Focal adhesion kinase stabilizes the cytoskeleton.

abstract：:Fission fragment tracks from uranium-stained biological preparations have been observed in the electron microscope in a study of the feasibility of their use as tracers. Virus, DNA, and tissue sections were stained with uranium, prepared for electron microscopy, irradiated in the high thermal neutron flux of a nuclear...

journal_title：Biophysical journal

authors： MALMON AG

更新日期：1964-01-01 00:00:00

abstract：:The nuclei of epididymal sperm, isolated from C57BL/6J and CBA/J inbred mice by their resistance to trypsin digestion, retain the shape differences of the intact sperm head. Various physical characteristics of these nuclei were measured and compared. The measurement of the projected dimensions of nuclei showed that th...

journal_title：Biophysical journal

authors： Wyrobek AJ,Meistrich ML,Furrer R,Bruce WR

更新日期：1976-07-01 00:00:00

abstract：:A block normal mode (BNM) algorithm, originally proposed by Tama et al., (Proteins Struct. Func. Genet. 41:1-7, 2000) was implemented into the simulation program CHARMM. The BNM approach projects the hessian matrix into local translation/rotation basis vectors and, therefore, dramatically reduces the size of the matri...

journal_title：Biophysical journal

authors： Li G,Cui Q

更新日期：2002-11-01 00:00:00

abstract：:Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These resu...

journal_title：Biophysical journal

authors： Sikorav JL,Pelta J,Livolant F

更新日期：1994-10-01 00:00:00

abstract：:We describe a model that relates the maximum shortening velocity of a muscle fiber, Vm, to the kinetics of the dissociation of a myosin head from actin. At Vm, the positive work exerted by cross-bridges attached in the powerstroke must be balanced by cross-bridges that have been carried by movement of the filaments in...

journal_title：Biophysical journal

authors： Cooke R,White H,Pate E

更新日期：1994-03-01 00:00:00

abstract：:The shape distributions of normal and hardened human and rabbit erythrocytes and platelets were obtained for edge-on orientations of a few hundred freely rotating cells from analyses of microphotographs obtained similarly as by Ponder(1930, Q. J. Exp. Physiol. 20:29) by phase-contrast microscopy at 800 X magnification...

journal_title：Biophysical journal

authors： Frojmovic MM,Panjwani R

更新日期：1976-09-01 00:00:00

abstract：:Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linke...

journal_title：Biophysical journal

authors： Neupane K,Woodside MT

更新日期：2016-07-26 00:00:00

abstract：:Bacteriorhodopsin proteoliposomes were used as a model system to explore the applicability of micromechanical cantilever arrays to detect conformational changes in membrane protein patches. The three main results of our study concern: 1), reliable functionalization of micromechanical cantilever arrays with proteolipos...

journal_title：Biophysical journal

authors： Braun T,Backmann N,Vögtli M,Bietsch A,Engel A,Lang HP,Gerber C,Hegner M

更新日期：2006-04-15 00:00:00

abstract：:Spin-label saturation transfer EPR (ST-EPR) methods have been used to study the sickle hemoglobin (HbS) aggregation behaviors induced by slow deoxygenation at a constant temperature of 30 degrees C, and by a rapid temperature increase from 1 degree to 30 degrees C for fully deoxygenated HbS. For slow deoxygenation at ...

journal_title：Biophysical journal

authors： Thiyagarajan P,Johnson ME

更新日期：1983-06-01 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:A refined mesoscopic model for the cumulative effect of repulsive excluded volume protein-protein interaction and attractive protein-surface interaction upon the properties of a trace protein capable of fiber formation is presented. The model predicts that very small changes in the magnitude of bulk volume occupancy o...

journal_title：Biophysical journal

authors： Minton AP

更新日期：2019-11-05 00:00:00

abstract：:Voltage-sensing dyes and voltage-sensing fluorescence proteins have been continually improved and as a result have provided a wealth of insights into neuronal circuits. Further improvements in voltage-sensing dyes and voltage-sensing fluorescence proteins are needed, however, for routine detection of single action pot...

journal_title：Biophysical journal

authors： Park K,Weiss S

更新日期：2017-02-28 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:The physical mechanism underlying the voltage-dependent gating of K channels is usually addressed theoretically using molecular dynamics simulations. However, besides being computationally very expensive, this approach is presently unable to fully predict the behavior of fundamental variables of channel gating such as...

journal_title：Biophysical journal

authors： Catacuzzeno L,Franciolini F

更新日期：2019-11-19 00:00:00

abstract：:Cells in a cloned population of coliform bacteria exhibit a wide range of swimming behaviors--a form of non-genetic individuality. We used computer models to examine the proposition that these variations are due to differences in the number of chemotaxis signaling molecules from one cell to the next. Simulations were ...

journal_title：Biophysical journal

authors： Levin MD,Morton-Firth CJ,Abouhamad WN,Bourret RB,Bray D

更新日期：1998-01-01 00:00:00

abstract：:We aimed to investigate insulin amyloid fibril polymorphism caused by salt effects and heating temperature and to visualize the structural differences of the polymorphisms in situ using Raman imaging without labeling. The time course monitoring for amyloid formation was carried out in an acidic condition without any s...

journal_title：Biophysical journal

authors： Ishigaki M,Morimoto K,Chatani E,Ozaki Y

更新日期：2020-06-16 00:00:00

abstract：:How do Ca channels conduct Ca ions during the cardiac action potential? We attempt to answer this question by applying a two-microelectrode technique, previously used for Na and K currents, in which we record the patch current and the action potential at the same time (Mazzanti, M., and L. J. DeFelice. 1987. Biophys. ...

journal_title：Biophysical journal

authors： Mazzanti M,DeFelice LJ

更新日期：1990-10-01 00:00:00

abstract：:The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical...

journal_title：Biophysical journal

authors： Dean KM,Roudot P,Welf ES,Danuser G,Fiolka R

更新日期：2015-06-16 00:00:00

abstract：:Using polarography and suitable mathematical analysis, the oxygen permeability of an individual layer of a bilayered tissue may be determined if the oxygen consumption and permeability of the other layer are known. Results obtained on rabbit cornea indicate that the permeability of the epithelium is about four times t...

journal_title：Biophysical journal

authors： Freeman RD,Fatt I

更新日期：1972-03-01 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00

abstract：:Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly...

journal_title：Biophysical journal

authors： Seeliger D,de Groot BL

更新日期：2010-05-19 00:00:00

abstract：:Cellular plasma membranes have domains that are defined, in most cases, by cytoskeletal elements. The outer half of the bilayer may also contain domains that organize glycosylphosphatidylinositol (GPI)-linked proteins. To define outer membrane barriers, we measured the resistive force on membrane bound beads as they w...

journal_title：Biophysical journal

authors： Suzuki K,Sterba RE,Sheetz MP

更新日期：2000-07-01 00:00:00

abstract：:Gaining information about receptor profiles on cells, and subsequently finding the most efficient ligands for these signaling receptors, remain challenging tasks in stem cell and cancer research as well as drug development. We introduce a live-cell method with great potential in both screening for surface receptors an...

journal_title：Biophysical journal

authors： Wienken U,Gaub HE

更新日期：2013-12-17 00:00:00

abstract：:Freeze-fracture electron micrographs from degranulating cells show that the lumen of the secretory granule is connected to the extracellular compartment via large (20 to 150 nm diameter) aqueous pores. These exocytotic fusion pores appear to be made up of a highly curved bilayer that spans the plasma and granule membr...

journal_title：Biophysical journal

authors： Nanavati C,Markin VS,Oberhauser AF,Fernandez JM

更新日期：1992-10-01 00:00:00

abstract：:Proteins must be stable to accomplish their biological function and to avoid enzymatic degradation. Constitutive proteolysis, however, is the main source of free amino acids used for de novo protein synthesis. In this paper the delicate balance of protein stability and degradability is discussed in the context of func...

journal_title：Biophysical journal

authors： Simon A,Dosztányi Zs,Rajnavölgyi E,Simon I

更新日期：2000-11-01 00:00:00

abstract：:Biological systems often have to measure extremely low concentrations of chemicals with high precision. When dealing with such small numbers of molecules, the inevitable randomness of physical transport processes and binding reactions will limit the precision with which measurements can be made. An important question ...

journal_title：Biophysical journal

authors： Kaizu K,de Ronde W,Paijmans J,Takahashi K,Tostevin F,ten Wolde PR

更新日期：2014-02-18 00:00:00

abstract：:Coarse-grained (CG) models of biomolecules have recently attracted considerable interest because they enable the simulation of complex biological systems on length-scales and timescales that are inaccessible for atomistic molecular dynamics simulation. A CG model is defined by a map that transforms an atomically detai...

journal_title：Biophysical journal

authors： Zhang Z,Lu L,Noid WG,Krishna V,Pfaendtner J,Voth GA

更新日期：2008-12-01 00:00:00

abstract：:Polypeptide toxins isolated from the venom of cone snails, known as μ-conotoxins, block voltage-gated sodium channels by physically occluding the ion-conducting pathway. Using molecular dynamics, we show that one subtype of μ-conotoxins, PIIIA, effectively blocks the bacterial voltage-gated sodium channel Na(V)Ab, who...

journal_title：Biophysical journal

authors： Chen R,Chung SH

更新日期：2012-02-08 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation with fluorescence detection has emerged as a powerful method for the study of interacting systems of macromolecules. It combines picomolar sensitivity with high hydrodynamic resolution, and can be carried out with photoswitchable fluorophores for multicomponent dis...

journal_title：Biophysical journal

authors： Chaturvedi SK,Zhao H,Schuck P

更新日期：2017-04-11 00:00:00

abstract：:The interaction between actin and myosin in the filament array of glycerinated muscle fibers has been monitored using paramagnetic probes and mechanical measurements. Both fiber stiffness and the spectra of probes bound to a reactive sulfydral on the myosin head were measured as the actomyosin bond was weakened by add...

journal_title：Biophysical journal

authors： Pate E,Cooke R

更新日期：1988-04-01 00:00:00