What Mutagenesis Can and Cannot Reveal About Allostery.

abstract：:The effects of rigor and cycling cross-bridges on distributions of calcium (Ca) bound within sarcomeres of rabbit psoas muscle fibers were compared using electron probe x-ray microanalysis. Calcium in the overlap region of rigor fibers, after correction for that bound to thick filaments, was significantly higher than ...

journal_title：Biophysical journal

authors： Cantino ME,Quintanilla A

更新日期：2007-01-15 00:00:00

abstract：:Presented here is a quantitative model of the wrapping and unwrapping of the DNA around the histone core of the nucleosome that suggests a mechanism by which this transition can be controlled: alteration of the charge state of the globular histone core. The mechanism is relevant to several classes of posttranslational...

journal_title：Biophysical journal

authors： Fenley AT,Adams DA,Onufriev AV

更新日期：2010-09-08 00:00:00

abstract：:A model for the Na-K exchange pump was applied to data on Na(+)-loaded frog sartorius muscle, and was used to relate the rate of adenosine triphosphate (ATP) hydrolysis to the electrical properties of the cell membrane. Membrane hyperpolarization was considered to arise from an electrical current which was produced by...

journal_title：Biophysical journal

authors： Rapoport SI

更新日期：1971-08-01 00:00:00

abstract：:A detailed electron spin resonance (ESR) study of mixtures of 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and phosphatidylserine (POPS) in oriented multilayers in the liquid crystalline phase is reported with the purpose of characterizing the effects of headgroup mixing on the structural and dynamical properties o...

journal_title：Biophysical journal

authors： Ge M,Budil DE,Freed JH

更新日期：1994-05-01 00:00:00

abstract：:Iberiotoxin, a toxin purified from the scorpion Buthus tamulus is a 37 amino acid peptide having 68% homology with charybdotoxin. Charybdotoxin blocks large conductance Ca(2+)-activated K+ channels at nanomolar concentrations from the external side only (Miller, C., E. Moczydlowski, R. Latorre, and M. Phillips. 1985. ...

journal_title：Biophysical journal

authors： Candia S,Garcia ML,Latorre R

更新日期：1992-08-01 00:00:00

abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

journal_title：Biophysical journal

authors： Anishkin A,Sukharev S,Colombini M

更新日期：2006-04-01 00:00:00

abstract：:Self-aggregation of isolated plant light-harvesting complexes (LHCs) upon detergent extraction is associated with fluorescence quenching and is used as an in vitro model to study the photophysical processes of nonphotochemical quenching (NPQ). In the NPQ state, in vivo induced under excess solar light conditions, harm...

journal_title：Biophysical journal

authors： Pandit A,Shirzad-Wasei N,Wlodarczyk LM,van Roon H,Boekema EJ,Dekker JP,de Grip WJ

更新日期：2011-11-16 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:Understanding how polypeptides can efficiently and reproducibly attain a self-entangled conformation is a compelling biophysical challenge that might shed new light on our general knowledge of protein folding. Complex lassos, namely self-entangled protein structures characterized by a covalent loop sealed by a cystein...

journal_title：Biophysical journal

authors： Perego C,Potestio R

更新日期：2019-07-23 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:The structure and alignment of the amphipathic alpha-helical antimicrobial peptide PGLa in a lipid membrane is determined with high accuracy by solid-state 2H-NMR. Orientational constraints are derived from a series of eight alanine-3,3,3-d3-labeled peptides, in which either a native alanine is nonperturbingly labeled...

journal_title：Biophysical journal

authors： Strandberg E,Wadhwani P,Tremouilhac P,Dürr UH,Ulrich AS

更新日期：2006-03-01 00:00:00

abstract：:Herein, we consider the process of force development along the adhesome within cell focal adhesions. Our model adhesome consists of the actin cytoskeleton-vinculin-talin-integrin-ligand-extracellular matrix-substrate force train. We specifically consider the effects of substrate stiffness on the force levels expected ...

journal_title：Biophysical journal

authors： Asaro RJ,Lin K,Zhu Q

更新日期：2019-11-05 00:00:00

abstract：:Raman spectroscopic data indicate that the conformations of the two hydrocarbon chains of dipalmitoyl phosphatidylcholine in aqueous dispersions of the lipid differ signficantly. The compounds 1-palmitoyl, 2-palmitoyl-d31-3-sn-phosphatidylcholine and 1-palmitoyl-d31, 2-palmitoyl-3-sn-phosphatidylcholine were synthesiz...

journal_title：Biophysical journal

authors： Gaber BP,Yager P,Peticolas WL

更新日期：1978-12-01 00:00:00

abstract：:The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) ...

journal_title：Biophysical journal

authors： Qiu L,Lewis A,Como J,Vaughn MW,Huang J,Somerharju P,Virtanen J,Cheng KH

更新日期：2009-05-20 00:00:00

abstract：:Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective observation of coordinated spontaneous fluctuations in two separate detection channels that unambiguously reflect the existence o...

journal_title：Biophysical journal

authors： Heinze KG,Rarbach M,Jahnz M,Schwille P

更新日期：2002-09-01 00:00:00

abstract：:Electrostatics-based calculations have been performed to examine the proton uptake upon reduction of the terminal electron acceptor Q(B) in the photosynthetic reaction center of Rhodobacter sphaeroides as a function of pH and the associated conformational equilibrium. Two crystal structures of the reaction center were...

journal_title：Biophysical journal

authors： Taly A,Sebban P,Smith JC,Ullmann GM

更新日期：2003-03-01 00:00:00

abstract：:Viral fusion peptides are short N-terminal regions of type-1 viral fusion proteins that are critical for virus entry. Although the importance of viral fusion peptides in virus-cell membrane fusion is established, little is known about how they function. We report the effects of wild-type (WT) hemagglutinin (HA) fusion...

journal_title：Biophysical journal

authors： Chakraborty H,Tarafdar PK,Klapper DG,Lentz BR

更新日期：2013-12-03 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00

abstract：:The cardiac muscle protein titin, responsible for developing passive elasticity and extensibility of muscle, possesses about 40 immunoglobulin-like (Ig) domains in its I-band region. Atomic force microscopy (AFM) and steered molecular dynamics (SMD) have been successfully combined to investigate the reversible unfoldi...

journal_title：Biophysical journal

authors： Gao M,Wilmanns M,Schulten K

更新日期：2002-12-01 00:00:00

abstract：:The oligomerization of the plasma membrane calcium pump (PMCA) in phospholipid/detergent micelles was evaluated using a combined spectroscopic and kinetic approach and related to the enzyme stability. Energy transfer between fluorescein-5'-isothiocyanate and eosin-5'-isothiocyanate attached to different PMCA molecules...

journal_title：Biophysical journal

authors： Levi V,Rossi JP,Castello PR,González Flecha FL

更新日期：2002-01-01 00:00:00

abstract：:Unlike most transmembrane proteins, phospholipids can migrate from one leaflet of the membrane to the other. Because this spontaneous lipid translocation (flip-flop) tends to be very slow, cells facilitate the process with enzymes that catalyze the transmembrane movement and thereby regulate the transbilayer lipid dis...

journal_title：Biophysical journal

authors： Doktorova M,Heberle FA,Marquardt D,Rusinova R,Sanford RL,Peyear TA,Katsaras J,Feigenson GW,Weinstein H,Andersen OS

更新日期：2019-03-05 00:00:00

abstract：:The phenomena reported are part of a study about the effects of ionizing radiation on membrane transport. We found that the conductance of lipid membranes in the presence of the polyene-antibiotics nystatin or amphotericin B is reduced to virtually zero following irradiation. Ion channels formed by these substances se...

journal_title：Biophysical journal

authors： Barth C,Stark G,Wilhelm M

更新日期：1993-01-01 00:00:00

abstract：:The lateral mobility of the lipid analog N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine and of the integral protein glycophorin in giant dimyristoylphosphatidylcholine vesicles was studied by the photobleaching technique. Above the temperature of the chain-melting transition (Tm = 23 degrees C), the diffusi...

journal_title：Biophysical journal

authors： Kapitza HG,Rüppel DA,Galla HJ,Sackmann E

更新日期：1984-03-01 00:00:00

abstract：:Cell adhesion often occurs under dynamic conditions, as in flowing blood. A quantitative understanding of this process requires accurate knowledge of the topographical relationships between the cell membrane and potentially adhesive surfaces. This report describes an experimental study made on both the translational a...

journal_title：Biophysical journal

authors： Tissot O,Pierres A,Foa C,Delaage M,Bongrand P

更新日期：1992-01-01 00:00:00

abstract：:Ionic channels bathed in mixed solutions of two permeant electrolytes often conduct less current than channels bathed in pure solutions of either. For many years, this anomalous mole fraction effect (AMFE) has been thought to occur only in single-file pores containing two or more ions at a time. Most thinking about ch...

journal_title：Biophysical journal

authors： Nonner W,Chen DP,Eisenberg B

更新日期：1998-05-01 00:00:00

abstract：:Proteins that cross-link actin filaments can either form bundles of parallel filaments or isotropic networks of individual filaments. We have found that mixtures of actin filaments with alpha-actinin purified from either Acanthamoeba castellanii or chicken smooth muscle can form bundles or isotropic networks depending...

journal_title：Biophysical journal

authors： Wachsstock DH,Schwartz WH,Pollard TD

更新日期：1993-07-01 00:00:00

abstract：:The suprachiasmatic nucleus governs daily variations of physiology and behavior in mammals. Within single neurons, interlocked transcriptional/translational feedback loops generate circadian rhythms on the molecular level. We present a mathematical model that reflects the essential features of the mammalian circadian ...

journal_title：Biophysical journal

authors： Becker-Weimann S,Wolf J,Herzel H,Kramer A

更新日期：2004-11-01 00:00:00

abstract：:Nonconfluent fibroblasts are relatively depolarized when compared with confluent fibroblasts, and transient hyperpolarizations result from a range of external stimuli as well as internal cellular activities. This electrical activity ceases, along with growth and mitogenic activity, when the cells become confluent. A c...

journal_title：Biophysical journal

authors： Stockbridge LL,French AS

更新日期：1988-07-01 00:00:00

abstract：:Immunocytochemical, biochemical, and molecular genetic studies indicate that apolipoprotein E (apoE) plays an important role in the process of amyloidogenesis-beta. However, there is still no clear translation of these data into the pathogenesis of amyloidosis-beta. Previous studies demonstrated sodium dodecyl sulfate...

journal_title：Biophysical journal

authors： Golabek AA,Kida E,Walus M,Perez C,Wisniewski T,Soto C

更新日期：2000-08-01 00:00:00

abstract：:Atomic force microscopy (AFM) has become an important tool for quantifying mechanical properties of biological materials ranging from single molecules to cells and tissues. Current AFM techniques for measuring elastic and viscoelastic properties of whole cells are based on indentation of cells firmly adhered to a subs...

journal_title：Biophysical journal

authors： Rosenbluth MJ,Lam WA,Fletcher DA

更新日期：2006-04-15 00:00:00