当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > An electron spin resonance study of interactions between phosphatidylcholine and phosphatidylserine in oriented membranes.

An electron spin resonance study of interactions between phosphatidylcholine and phosphatidylserine in oriented membranes.

Abstract：

:A detailed electron spin resonance (ESR) study of mixtures of 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and phosphatidylserine (POPS) in oriented multilayers in the liquid crystalline phase is reported with the purpose of characterizing the effects of headgroup mixing on the structural and dynamical properties of the acyl chains. These studies were performed over a range of blends of POPC and POPS and temperatures, utilizing the spin-labeled lipids 16-phosphatidylcholine and 5-phosphatidylcholine as well as cholestane (CSL). The ESR spectra were analyzed by nonlinear least-squares fitting using detailed spectral simulations. Whereas CSL shows almost no variation in ordering and rotational dynamics versus mole fraction POPS, (i.e. XPS), and 5-PC shows small effects, the weakly ordered end-chain labeled 16-PC shows large relative effects, such that the orientational order parameter, S is at a minimum for XPS = 0.5 where it is about one-third the value observed for XPS = 0 and 1. This is directly reflected in the ESR spectrum as a substantial variation in the hyperfine splitting with XPS. The least-squares analysis also shows a reduction in rotational diffusion coefficient, R perpendicular by a fractor of 2 for XPS = 0.5 and permits the estimation of S2, the ordering parameter representing deviations from cylindrically symmetric alignment. These results are contrasted with 2H NMR studies which were insensitive to effects of mixing headgroups on the acyl chains. The ESR results are consistent with a somewhat increased disorder in the end-chain region as well as a small amount of chain tilting upon mixing POPC and POPS. They demonstrate the high sensitivity of ESR to subtle effects in chain ordering and dynamics.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Ge M,Budil DE,Freed JH

doi

10.1016/S0006-3495(94)80942-7

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

1515-21

issue

5

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(94)80942-7

journal_volume

66

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • Vibrational neutron spectroscopy of collagen and model polypeptides.

    abstract：:A pulsed source neutron spectrometer has been used to measure vibrational spectra (20-4000 cm-1) of dry and hydrated type I collagen fibers, and of two model polypeptides, polyproline II and (prolyl-prolyl-glycine)10, at temperatures of 30 and 120 K. the collagen spectra provide the first high resolution neutron views...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)79942-8

    authors： Middendorf HD,Hayward RL,Parker SF,Bradshaw J,Miller A

    更新日期：1995-08-01 00:00:00

  • Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy.

    abstract：:Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linke...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.06.011

    authors： Neupane K,Woodside MT

    更新日期：2016-07-26 00:00:00

  • Human PIEZO1: removing inactivation.

    abstract：:PIEZO1 is an inactivating eukaryotic cation-selective mechanosensitive ion channel. Two sites have been located in the channel that when individually mutated lead to xerocytotic anemia by slowing inactivation. By introducing mutations at two sites, one associated with xerocytosis and the other artificial, we were able...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.07.019

    authors： Bae C,Gottlieb PA,Sachs F

    更新日期：2013-08-20 00:00:00

  • Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR.

    abstract：:The chemical shifts of specific (13)C and (15)N labels distributed throughout KIAGKIA-KIAGKIA-KIAGKIA (K3), an amphiphilic 21-residue antimicrobial peptide, prove that the peptide is in an all alpha-helical conformation in the bilayers of multilamellar vesicles (MLVs) containing dipalmitoylphosphatidylcholine and dipa...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.103.032706

    authors： Toke O,Maloy WL,Kim SJ,Blazyk J,Schaefer J

    更新日期：2004-07-01 00:00:00

  • Torsion profiling of proteins using magnetic particles.

    abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.01.027

    authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

    更新日期：2013-03-05 00:00:00

  • Analytical calculation of intracellular calcium wave characteristics.

    abstract：:We present a theoretical analysis of intracellular calcium waves propagated by calcium feedback at the inositol 1,4,5-trisphosphate (IP3) receptor. The model includes essential features of calcium excitability, but is still analytically tractable. Formulas are derived for the wave speed, amplitude, and width. The calc...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(97)78888-X

    authors： Kupferman R,Mitra PP,Hohenberg PC,Wang SS

    更新日期：1997-06-01 00:00:00

  • Excitation of squid giant axons below 0 degree C.

    abstract：:The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, w...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(81)84785-6

    authors： Kukita F,Yamagishi S

    更新日期：1981-07-01 00:00:00

  • A membrane model for cytosolic calcium oscillations. A study using Xenopus oocytes.

    abstract：:Cytosolic calcium oscillations occur in a wide variety of cells and are involved in different cellular functions. We describe these calcium oscillations by a mathematical model based on the putative electrophysiological properties of the endoplasmic reticulum (ER) membrane. The salient features of our membrane model a...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(92)81583-7

    authors： Jafri MS,Vajda S,Pasik P,Gillo B

    更新日期：1992-07-01 00:00:00

  • HIV-1 capsid protein forms spherical (immature-like) and tubular (mature-like) particles in vitro: structure switching by pH-induced conformational changes.

    abstract：:The viral genome and replicative enzymes of the human immunodeficiency virus are encased in a shell consisting of assembled mature capsid protein (CA). The core shell is a stable, effective protective barrier, but is also poised for dissolution on cue to allow transmission of the viral genome into its new host. In thi...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(01)75725-6

    authors： Ehrlich LS,Liu T,Scarlata S,Chu B,Carter CA

    更新日期：2001-07-01 00:00:00

  • Systematic size study of an insect antifreeze protein and its interaction with ice.

    abstract：:Because of their remarkable ability to depress the freezing point of aqueous solutions, antifreeze proteins (AFPs) play a critical role in helping many organisms survive subzero temperatures. The beta-helical insect AFP structures solved to date, consisting of multiple repeating circular loops or coils, are perhaps th...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.051169

    authors： Liu K,Jia Z,Chen G,Tung C,Liu R

    更新日期：2005-02-01 00:00:00

  • New model of charged molecule redistribution induced in spherical vesicles by direct current electric field.

    abstract：:A new electrophoresis model of charged components in a spherical phospholipid vesicle is proposed. In the new model the effective local tangential electric field is a result of the uniform external electric field modified by the electric field of redistributed charges. The modification is calculated on the basis of th...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/s0006-3495(97)78699-5

    authors： Piasecki W,Salwiński L,Froncisz W

    更新日期：1997-02-01 00:00:00

  • Continuous allosteric regulation of a viral packaging motor by a sensor that detects the density and conformation of packaged DNA.

    abstract：:We report evidence for an unconventional type of allosteric regulation of a biomotor. We show that the genome-packaging motor of phage ϕ29 is regulated by a sensor that detects the density and conformation of the DNA packaged inside the viral capsid, and slows the motor by a mechanism distinct from the effect of a dir...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2014.11.3469

    authors： Berndsen ZT,Keller N,Smith DE

    更新日期：2015-01-20 00:00:00

  • Slow and incomplete inactivations of voltage-gated channels dominate encoding in synthetic neurons.

    abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(93)81153-6

    authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

    更新日期：1993-09-01 00:00:00

  • A double-deletion method to quantifying incremental binding energies in proteins from experiment: example of a destabilizing hydrogen bonding pair.

    abstract：:The contribution of a specific hydrogen bond in apoflavodoxin to protein stability is investigated by combining theory, experiment and simulation. Although hydrogen bonds are major determinants of protein structure and function, their contribution to protein stability is still unclear and widely debated. The best meth...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.050203

    authors： Campos LA,Cuesta-López S,López-Llano J,Falo F,Sancho J

    更新日期：2005-02-01 00:00:00

  • Molecular dynamics free energy calculations to assess the possibility of water existence in protein nonpolar cavities.

    abstract：:Are protein nonpolar cavities filled with water molecules? Although many experimental and theoretical investigations have been done, particularly for the nonpolar cavity of IL-1 beta, the results are still conflicting. To study this problem from the thermodynamic point of view, we calculated hydration free energies of...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.01.029

    authors： Oikawa M,Yonetani Y

    更新日期：2010-06-16 00:00:00

  • Evidence for ligand-induced conformational changes in proteins from phosphorescence spectroscopy.

    abstract：:Phosphorescence spectroscopy on mouse myeloma IgA J539 in rigid solution at 77K revealed the type of anomalous short-lived component in the tryptophan decay originally observed with lysozyme (Churchich, J.E., 1966. Biochim. Biophys. Acta. 120:406-412) and seen in a large number of Bence Jones proteins (Longworth, J.W....

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(89)82681-5

    authors： Li Z,Galley WC

    更新日期：1989-08-01 00:00:00

  • Probing the orientational distribution of dyes in membranes through multiphoton microscopy.

    abstract：:Numerous dyes are available or under development for probing the structural and functional properties of biological membranes. Exogenous chromophores adopt a range of orientations when bound to membranes, which have a drastic effect on their biophysical behavior. Here, we present a method that employs optical anisotro...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2012.08.003

    authors： Reeve JE,Corbett AD,Boczarow I,Wilson T,Bayley H,Anderson HL

    更新日期：2012-09-05 00:00:00

  • Nanomechanical mapping of hydrated rat tail tendon collagen I fibrils.

    abstract：:Collagen fibrils play an important role in the human body, providing tensile strength to connective tissues. These fibrils are characterized by a banding pattern with a D-period of 67 nm. The proposed origin of the D-period is the internal staggering of tropocollagen molecules within the fibril, leading to gap and ove...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2014.09.003

    authors： Baldwin SJ,Quigley AS,Clegg C,Kreplak L

    更新日期：2014-10-21 00:00:00

  • Simultaneous measurement of Ca2+ influx and reversal potentials in recombinant N-methyl-D-aspartate receptor channels.

    abstract：:The Ca(2+) permeability of N-methyl-D-aspartate receptor (NMDA-R) channels was studied in human embryonic kidney cells transfected with the NR1-NR2A subunit combination. To determine the fractional Ca(2+) current (P(f)), measurements of fura-2-based Ca(2+) influx and whole-cell currents were made in symmetrical monova...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(96)79782-5

    authors： Schneggenburger R

    更新日期：1996-05-01 00:00:00

  • Investigation of N-Terminal Phospho-Regulation of Uracil DNA Glycosylase Using Protein Semisynthesis.

    abstract：:Uracil DNA Glycosylase (UNG2) is the primary enzyme in humans that prevents the stable incorporation of deoxyuridine monophosphate into DNA in the form of U/A basepairs. During S-phase, UNG2 remains associated with the replication fork through its interactions with two proteins, Proliferating Cell Nuclear Antigen (PCN...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.06.016

    authors： Weiser BP,Stivers JT,Cole PA

    更新日期：2017-07-25 00:00:00

  • Cartilage aggrecan can undergo self-adhesion.

    abstract：:Here it is reported that aggrecan, the highly negatively charged macromolecule in the cartilage extracellular matrix, undergoes Ca(2+)-mediated self-adhesion after static compression even in the presence of strong electrostatic repulsion in physiological-like solution conditions. Aggrecan was chemically end-attached o...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.128389

    authors： Han L,Dean D,Daher LA,Grodzinsky AJ,Ortiz C

    更新日期：2008-11-15 00:00:00

  • The nature of sources of bioelectric and biomagnetic fields.

    abstract：:The goal of this paper is to examine the origins and relative importance of primary and secondary sources of electric and magnetic fields for excitable tissue. It is shown that for axonal and cardiac tissue a comparison of the relative field strength from both primary and secondary sources shows only the latter to be ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(82)84521-9

    authors： Plonsey R

    更新日期：1982-09-01 00:00:00

  • Quantitative radiation effects in hemoproteins and their constituents.

    abstract：:In order to verify the "radiation protection" effect of prosthetic groups in irradiated conjugated proteins and the influence of their electronic structure on intramolecular excitation transfer, two series of substances were examined. The first was composed of ferrihemoglobin and its constituents and the second was co...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(70)86299-3

    authors： Crippa PR,Vecli A

    更新日期：1970-03-01 00:00:00

  • An analytic solution to the Monod-Wyman-Changeux model and all parameters in this model.

    abstract：:Starting from the Monod-Wyman-Changeux (MWC) model (Monod, J., J. Wyman, and J. P. Changeux. 1965. J. Mol. Biol. 12:88-118), we obtain an analytical expression for the slope of the Hill plot at any ligand concentration. Furthermore, we derive an equation satisfied by the ligand concentration at the position of maximum...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(89)82802-4

    authors： Zhou G,Ho PS,van Holde KE

    更新日期：1989-02-01 00:00:00

  • Electrochemical healing similarities between animals and plants.

    abstract：:A brief summary of the major results in enhanced wound healing by electrolysis in animals and humans is presented along with the results of enhanced growth by electrolysis in plants. Hypotheses of normal and enhanced wound healing in animal and plants are reviewed. A comparison of the experimental results indicates st...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(79)85230-3

    authors： Gensler W

    更新日期：1979-09-01 00:00:00

  • Searching for the molecular arrangement of transmembrane ceramide channels.

    abstract：:Ceramides have been implicated in the initiation of apoptosis by permeabilizing the mitochondrial outer membrane to small proteins, including cytochrome c. In addition, ceramides were shown to form large metastable channels in planar membranes and liposomes, indicating that these lipids permeabilize membranes directly...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.105.071977

    authors： Anishkin A,Sukharev S,Colombini M

    更新日期：2006-04-01 00:00:00

  • From static structure to living protein: computational analysis of cytochrome c oxidase main-chain flexibility.

    abstract：:Crystallographic structure and deuterium accessibility comparisons of CcO in different redox states have suggested conformational changes of mechanistic significance. To predict the intrinsic flexibility and low energy motions in CcO, this work has analyzed available high-resolution crystallographic structures with Pr...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2012.03.040

    authors： Buhrow L,Ferguson-Miller S,Kuhn LA

    更新日期：2012-05-02 00:00:00

  • Molecular mechanics of filamin's rod domain.

    abstract：:Rearrangement of the actin cytoskeleton is integral to cell shape and function. Actin-binding proteins, e.g., filamin, can naturally contribute to the mechanics and function of the actin cytoskeleton. The molecular mechanical bases for filamin's function in actin cytoskeletal reorganization are examined here using mol...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.118802

    authors： Kolahi KS,Mofrad MR

    更新日期：2008-02-01 00:00:00

  • Theoretical limits on the threshold for the response of long cells to weak extremely low frequency electric fields due to ionic and molecular flux rectification.

    abstract：:Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(98)77669-6

    authors： Weaver JC,Vaughan TE,Adair RK,Astumian RD

    更新日期：1998-11-01 00:00:00

  • Structural and functional similarities between the nucleotide-binding domains of CFTR and GTP-binding proteins.

    abstract：:The opening and closing of the CFTR Cl- channel are regulated by ATP hydrolysis at its two nucleotide binding domains (NBDs). However, the mechanism and functional significance of ATP hydrolysis are unknown. Sequence similarity between the NBDs of CFTR and GTP-binding proteins suggested the NBDs might have a structure...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)80113-X

    authors： Carson MR,Welsh MJ

    更新日期：1995-12-01 00:00:00