Searching for the molecular arrangement of transmembrane ceramide channels.

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:Recent NMR structural characterization studies showed that a seven-residue segment (FKKGERL) from the src SH3 domain adopts the nativelike diverging type II beta-turn in aqueous solution in support of the prediction based on the I-sites library of sequence structural motifs. We study the conformational variability and...

journal_title：Biophysical journal

authors： Gnanakaran S,Garcia AE

更新日期：2003-03-01 00:00:00

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00

abstract：:We studied the effects of permeant ions on the gating of the large conductance Ca(2+)-activated K+ channel from rat skeletal muscle. Rb+ blockade of inward K+ current caused an increase in the open probability as though Rb+ occupancy of the pore interferes with channel closing. In support of this hypothesis, we direct...

journal_title：Biophysical journal

authors： Demo SD,Yellen G

更新日期：1992-03-01 00:00:00

abstract：:Gastrointestinal slow waves are generated within networks of interstitial cells of Cajal (ICCs). In the intact tissue, slow waves are entrained to neighboring ICCs with higher intrinsic frequencies, leading to active propagation of slow waves. Degradation of ICC networks in humans is associated with motility disorders...

journal_title：Biophysical journal

authors： Du P,O'Grady G,Gibbons SJ,Yassi R,Lees-Green R,Farrugia G,Cheng LK,Pullan AJ

更新日期：2010-05-19 00:00:00

abstract：:In cardiac tissue, during partial blockade of the membrane sodium channels, or at high frequencies of excitation, inexcitable obstacles with sharp edges may destabilize the propagation of electrical excitation waves, causing the formation of self-sustained vortices and turbulent cardiac electrical activity. The format...

journal_title：Biophysical journal

authors： Cabo C,Pertsov AM,Davidenko JM,Baxter WT,Gray RA,Jalife J

更新日期：1996-03-01 00:00:00

abstract：:The cell membrane of Valonia utricularis contains an electrogenic carrier system for chloride (Wang et al., Biophys J. 59:235-248 (1991)). The electrical impedance of V. utricularis was measured in the frequency range between 1 Hz and 50 kHz. The analysis of the impedance spectra from V. utricularis and its comparison...

journal_title：Biophysical journal

authors： Wang J,Zimmermann U,Benz R

更新日期：1994-10-01 00:00:00

abstract：:In the intestinal epithelium, proliferated epithelial cells ascend the crypts and villi and shed at the villus tips into the gut lumen. In this study, we theoretically investigate the roles of the villi on cell turnover. We present a stochastic model that focuses on the duration over which cells migrate the shortest p...

journal_title：Biophysical journal

authors： Kai Y

更新日期：2021-01-13 00:00:00

abstract：:Two rate-limiting mechanisms have been proposed to explain the gramicidin channel facilitated decay of the pH difference across vesicular membrane (delta pH) in the pH region 6-8 and salt (MCI, M+ = K+, Na+) concentration range 50-300 mM. 1) At low pH conditions (approximately 6), H+ transport through the gramicidin c...

journal_title：Biophysical journal

authors： Prabhananda BS,Kombrabail MH

更新日期：1996-12-01 00:00:00

abstract：:Crystallographic structure and deuterium accessibility comparisons of CcO in different redox states have suggested conformational changes of mechanistic significance. To predict the intrinsic flexibility and low energy motions in CcO, this work has analyzed available high-resolution crystallographic structures with Pr...

journal_title：Biophysical journal

authors： Buhrow L,Ferguson-Miller S,Kuhn LA

更新日期：2012-05-02 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:Understanding how polypeptides can efficiently and reproducibly attain a self-entangled conformation is a compelling biophysical challenge that might shed new light on our general knowledge of protein folding. Complex lassos, namely self-entangled protein structures characterized by a covalent loop sealed by a cystein...

journal_title：Biophysical journal

authors： Perego C,Potestio R

更新日期：2019-07-23 00:00:00

abstract：:The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

journal_title：Biophysical journal

authors： Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

更新日期：2009-01-01 00:00:00

abstract：:A detailed model for the kinetics and energetics of the exciton trapping, charge separation, charge recombination, and charge stabilization processes in photosystem (PS) II is presented. The rate constants describing these processes in open and closed reaction centers (RC) are calculated on the basis of picosecond dat...

journal_title：Biophysical journal

authors： Schatz GH,Brock H,Holzwarth AR

更新日期：1988-09-01 00:00:00

abstract：:Results are presented of nonphotochemical-hole-burning experiments on the mitochondrial specific dye rhodamine 800 incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV167 carcinoma cells. This dye is selective for the plasma and inner membranes of the mitochondria, as shown b...

journal_title：Biophysical journal

authors： Walsh RJ,Reinot T,Hayes JM,Kalli KR,Hartmann LC,Small GJ

更新日期：2003-02-01 00:00:00

abstract：:Connectin (titin) is a large filamentous protein (single peptide) with a molecular mass of approximately 3 MDa, contour length approximately 900 nm, and diameter approximately 4 nm, and resides in striated muscle. Connectin links the thick filaments to the Z-lines in a sarcomere and produces a passive elastic force wh...

journal_title：Biophysical journal

authors： Higuchi H,Nakauchi Y,Maruyama K,Fujime S

更新日期：1993-11-01 00:00:00

abstract：:In this article, we explore the information content of molecular force-field calculations. We make use of exhaustive lattice models of molecular conformations and reduced alphabet sequences to determine the relative resolving power of pairwise interaction-based force fields. We find that sequence-specific interactions...

journal_title：Biophysical journal

authors： Aynechi T,Kuntz ID

更新日期：2005-11-01 00:00:00

abstract：:We have analyzed flash-induced period-four damped oscillation of oxygen evolution and chlorophyll fluorescence with the aid of a kinetic model of photosystem II. We have shown that, for simulation of the period-four oscillatory behavior of oxygen evolution, it is essential to consider the so-called intermediate S-stat...

journal_title：Biophysical journal

authors： Jablonsky J,Lazar D

更新日期：2008-04-01 00:00:00

abstract：:We introduce an approach based on the recently introduced functional mode analysis to identify collective modes of internal dynamics that maximally correlate to an external order parameter of functional interest. Input structural data can be either experimentally determined structure ensembles or simulated ensembles, ...

journal_title：Biophysical journal

authors： Krivobokova T,Briones R,Hub JS,Munk A,de Groot BL

更新日期：2012-08-22 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin (bR) from Halobacterium salinarum mutant L93A, the O-intermediate accumulates and the cycling time is increased approximately 200 times. Nevertheless, under continuous illumination, the protein pumps protons at near wild-type rates. We excited the mutant L93A in purple membrane w...

journal_title：Biophysical journal

authors： Tóth-Boconádi R,Keszthelyi L,Stoeckenius W

更新日期：2003-06-01 00:00:00

abstract：:FKBP12 was removed from ryanodine receptors (RyRs) by incubation of rabbit skeletal muscle terminal cisternae membranes with rapamycin. The extent of FKBP12 removal was estimated by immunostaining Western blots of terminal cisternae proteins. Single FKBP12-depleted RyR channels, incorporated into planar lipid bilayers...

journal_title：Biophysical journal

authors： Ahern GP,Junankar PR,Dulhunty AF

更新日期：1997-01-01 00:00:00

abstract：:In vitro and in situ tests have been run to characterize the reaction of the mettalochromic indicator, arsenazo III, with calcium. Job plots as well as plots of indicator absorbance vs. [Ca2+] at different indicator concentrations show a 1:1 reaction stoichiometry. Equilibrium analysis and analysis using Adair's equat...

journal_title：Biophysical journal

authors： Ahmed Z,Kragie L,Connor JA

更新日期：1980-12-01 00:00:00

abstract：:The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic downshift. It has been identified in Escherichia coli as a product of the orphan yggB gene, now called mscS (Levina et al., 1999, EMBO J. 18:1730). Here I show that th...

journal_title：Biophysical journal

authors： Sukharev S

更新日期：2002-07-01 00:00:00

abstract：:The photocycle of the photophobic receptor from Natronobacterium pharaonis, NpSRII, is studied by static and time-resolved step-scan Fourier transform infrared spectroscopy. Both low-temperature static and time-resolved spectra resolve a K-like intermediate, and the corresponding spectra show little difference within ...

journal_title：Biophysical journal

authors： Hein M,Wegener AA,Engelhard M,Siebert F

更新日期：2003-02-01 00:00:00

abstract：:Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuse...

journal_title：Biophysical journal

authors： Mashl RJ,Bruinsma RF

更新日期：1998-06-01 00:00:00

abstract：:The energy transfer and charge separation kinetics of a photosystem I (PS I) core particle of an antenna size of 100 chlorophyll/P700 has been studied by combined fluorescence and transient absorption kinetics with picosecond resolution. This is the first combined picosecond study of transient absorption and fluoresce...

journal_title：Biophysical journal

authors： Holzwarth AR,Schatz G,Brock H,Bittersmann E

更新日期：1993-06-01 00:00:00

abstract：:The conformational states of Escherichia coli Rep helicase undergoing ATP hydrolysis while bound to a partial-duplex DNA (pdDNA) were studied using single-molecule FRET. Crystallographic studies showed that Rep bound to single-stranded DNA can exist in open and closed conformations that differ in the orientation of th...

journal_title：Biophysical journal

authors： Balci H,Arslan S,Myong S,Lohman TM,Ha T

更新日期：2011-08-17 00:00:00

abstract：:The functions and elasticities of the cell are largely related to the structures of the cytoskeletons underlying the lipid bilayer. Among various cell types, the red blood cell (RBC) possesses a relatively simple cytoskeletal structure. Underneath the membrane, the RBC cytoskeleton takes the form of a two-dimensional ...

journal_title：Biophysical journal

authors： Lai L,Xu X,Lim CT,Cao J

更新日期：2015-12-01 00:00:00