Spontaneous-curvature theory of clathrin-coated membranes.


:Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuser (J. Cell Biol. 108:401-411) that suggest the transformation proceeds by changes in the chemical environment of the clathrin lattice, wherein the chemical environment determines the amount of intrinsic, or spontaneous, curvature of the network. We show that a necessary step of the transformation, formation of free pentagons in the clathrin network, can proceed via dislocation unbinding, driven by changes in the spontaneous curvature. Dislocation unbinding is shown to favor formation of coated vesicles that are quite small compared to those predicted by the current continuum theories, which do not include the topology of the clathrin lattice.


Biophys J


Biophysical journal


Mashl RJ,Bruinsma RF




Has Abstract


1998-06-01 00:00:00














  • Effects of pH on myofibrillar ATPase activity in fast and slow skeletal muscle fibers of the rabbit.

    abstract::In permeabilized single fibers of fast (psoas) and slow (soleus) muscle from the rabbit, the effect of pH on isometric myofibrillar ATPase activity and force was studied at 15 degrees C, in the pH range 6.4-7.9. ATPase activity was measured photometrically by enzymatic coupling of the regeneration of ATP to the oxidat...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Potma EJ,van Graas IA,Stienen GJ

    更新日期:1994-12-01 00:00:00

  • Probing vesicle dynamics in single hippocampal synapses.

    abstract::We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicl...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XL

    更新日期:2005-11-01 00:00:00

  • Attempts to define functional domains of gap junction proteins with synthetic peptides.

    abstract::To map the binding sites involved in channel formation, synthetic peptides representing sequences of connexin 32 were tested for their ability to inhibit cell-cell channel formation. Both large peptides representing most of the two presumed extracellular loops of connexin32 and shorter peptides representing subsets of...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dahl G,Nonner W,Werner R

    更新日期:1994-11-01 00:00:00

  • Elementary events underlying voltage-dependent G-protein inhibition of N-type calcium channels.

    abstract::Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Patil PG,de Leon M,Reed RR,Dubel S,Snutch TP,Yue DT

    更新日期:1996-11-01 00:00:00

  • Role of Phosphorylation in Moesin Interactions with PIP2-Containing Biomimetic Membranes.

    abstract::Moesin, a protein of the ezrin, radixin, and moesin family, which links the plasma membrane to the cytoskeleton, is involved in multiple physiological and pathological processes, including viral budding and infection. Its interaction with the plasma membrane occurs via a key phosphoinositide, the phosphatidyl(4,5)inos...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lubart Q,Vitet H,Dalonneau F,Le Roy A,Kowalski M,Lourdin M,Ebel C,Weidenhaupt M,Picart C

    更新日期:2018-01-09 00:00:00

  • Examination of optical depth effects on fluorescence imaging of cardiac propagation.

    abstract::Optical mapping with voltage-sensitive dyes provides a high-resolution technique to observe cardiac electrodynamic behavior. Although most studies assume that the fluorescent signal is emitted from the surface layer of cells, the effects of signal attenuation with depth on signal interpretation are still unclear. This...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bray MA,Wikswo JP

    更新日期:2003-12-01 00:00:00

  • Computer modelling of glycolipids at membrane surfaces.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ram P,Kim E,Thomson DS,Howard KP,Prestegard JH

    更新日期:1992-12-01 00:00:00

  • Combined affinity and rate constant distributions of ligand populations from experimental surface binding kinetics and equilibria.

    abstract::The present article considers the influence of heterogeneity in a mobile analyte or in an immobilized ligand population on the surface binding kinetics and equilibrium isotherms. We describe strategies for solving the inverse problem of calculating two-dimensional distributions of rate and affinity constants from expe...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Svitel J,Balbo A,Mariuzza RA,Gonzales NR,Schuck P

    更新日期:2003-06-01 00:00:00

  • Proton transport through influenza A virus M2 protein reconstituted in vesicles.

    abstract::Influenza A virus M2 protein is known to form acid-activated, proton-selective, amantadine-sensitive channels. We directly measured proton uptake in vesicles containing reconstituted M2 by monitoring external pH after addition of valinomycin to vesicles with 100-fold-diluted external [K(+)]. External pH typically incr...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Moffat JC,Vijayvergiya V,Gao PF,Cross TA,Woodbury DJ,Busath DD

    更新日期:2008-01-15 00:00:00

  • Intrinsic shortening speed of temperature-jump-activated intact muscle fibers. Effects of varying osmotic pressure with sucrose and KCl.

    abstract::Effects of intracellular ionic strength on the isotonic contraction properties of both intact fibers and skinned fibers give insights into the cross-bridge mechanism, but presently there is fundamental disagreement in the results on the two fiber preparations. This paper, which studies the effects on contraction of va...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gulati J,Babu A

    更新日期:1984-02-01 00:00:00

  • Myoglobin and hemoglobin rotational diffusion in the cell.

    abstract::The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Wang D,Kreutzer U,Chung Y,Jue T

    更新日期:1997-11-01 00:00:00

  • Anomalous mole fraction effect, electrostatics, and binding in ionic channels.

    abstract::Ionic channels bathed in mixed solutions of two permeant electrolytes often conduct less current than channels bathed in pure solutions of either. For many years, this anomalous mole fraction effect (AMFE) has been thought to occur only in single-file pores containing two or more ions at a time. Most thinking about ch...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Nonner W,Chen DP,Eisenberg B

    更新日期:1998-05-01 00:00:00

  • Modulation of nanotube formation by structural modifications of sphingolipids.

    abstract::Galactosylceramides (GalCers) containing nervonoyl (24:1(Delta15(cis))) acyl chains have the capacity to assemble into nanotubular microstructures in excess water (. Biophys. J. 69:1976-1986). To define the structural parameters that modulate nanotube formation, GalCer derivatives were synthesized that contained cis m...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kulkarni VS,Boggs JM,Brown RE

    更新日期:1999-07-01 00:00:00

  • Modeling feedback loops of the Mammalian circadian oscillator.

    abstract::The suprachiasmatic nucleus governs daily variations of physiology and behavior in mammals. Within single neurons, interlocked transcriptional/translational feedback loops generate circadian rhythms on the molecular level. We present a mathematical model that reflects the essential features of the mammalian circadian ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Becker-Weimann S,Wolf J,Herzel H,Kramer A

    更新日期:2004-11-01 00:00:00

  • Actin modifies Ca2+ block of epithelial Na+ channels in planar lipid bilayers.

    abstract::The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Berdiev BK,Latorre R,Benos DJ,Ismailov II

    更新日期:2001-05-01 00:00:00

  • Effects of codon distributions and tRNA competition on protein translation.

    abstract::Translation is a central cellular process and the complexity of its mechanism necessitates mathematical frameworks to better understand system properties and make quantitative predictions. We have developed a gene sequence-specific mechanistic model for translation which accounts for all the elementary steps of transl...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zouridis H,Hatzimanikatis V

    更新日期:2008-08-01 00:00:00

  • Excited state trapping and the Stepanov relation with reference to Photosystem I.

    abstract::It has been previously demonstrated that the Stepanov equation provides a rather good description of the absorption/fluorescence spectra in Photosystem I, even though excited state equilibration is not rapid with respect to the excited state decay. In the present article this apparent contradiction is examined analyti...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jennings RC,Garlaschi FM,Zucchelli G

    更新日期:2003-12-01 00:00:00

  • Spiral Wave Propagation in Communities with Spatially Correlated Heterogeneity.

    abstract::Many multicellular communities propagate signals in a directed manner via excitable waves. Cell-to-cell heterogeneity is a ubiquitous feature of multicellular communities, but the effects of heterogeneity on wave propagation are still unclear. Here, we use a minimal FitzHugh-Nagumo-type model to investigate excitable ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zhai X,Larkin JW,Süel GM,Mugler A

    更新日期:2020-04-07 00:00:00

  • The Latest Twists in Chromatin Remodeling.

    abstract::In its most restrictive interpretation, the notion of chromatin remodeling refers to the action of chromatin-remodeling enzymes on nucleosomes with the aim of displacing and removing them from the chromatin fiber (the effective polymer formed by a DNA molecule and proteins). This local modification of the fiber struct...

    journal_title:Biophysical journal

    pub_type: 杂志文章,评审


    authors: Blossey R,Schiessel H

    更新日期:2018-05-22 00:00:00

  • Compartmentalization of the outer hair cell demonstrated by slow diffusion in the extracisternal space.

    abstract::In the outer hair cell (OHC), the extracisternal space (ECiS) is a conduit and reservoir of the molecular and ionic substrates of the lateral wall, including those necessary for electromotility. To determine the mechanisms through which molecules are transported in the ECiS of the OHC, we selectively imaged the time-d...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gliko O,Saggau P,Brownell WE

    更新日期:2009-08-19 00:00:00

  • Differential stability of beta-sheets and alpha-helices in beta-lactamase: a high temperature molecular dynamics study of unfolding intermediates.

    abstract::beta-Lactamase, which catalyzes beta-lactam antibiotics, is prototypical of large alpha/beta proteins with a scaffolding formed by strong noncovalent interactions. Experimentally, the enzyme is well characterized, and intermediates that are slightly less compact and having nearly the same content of secondary structur...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vijayakumar S,Vishveshwara S,Ravishanker G,Beveridge DL

    更新日期:1993-12-01 00:00:00

  • A cellular screening assay using analysis of metal-modified fluorescence lifetime.

    abstract::Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with superresolution axial sensitivity and no need for image analysis. We sh...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cade NI,Fruhwirth G,Archibald SJ,Ng T,Richards D

    更新日期:2010-06-02 00:00:00

  • Sorting of lipids and proteins in membrane curvature gradients.

    abstract::The sorting of lipids and proteins in cellular trafficking pathways is a process of central importance in maintaining compartmentalization in eukaryotic cells. However, the mechanisms behind these sorting phenomena are currently far from being understood. Among several mechanistic suggestions, membrane curvature has b...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tian A,Baumgart T

    更新日期:2009-04-08 00:00:00

  • A computational framework for mechanical response of macromolecules: application to the salt concentration dependence of DNA bendability.

    abstract::A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ma L,Yethiraj A,Chen X,Cui Q

    更新日期:2009-05-06 00:00:00

  • A theoretical analysis for the effect of focal contact formation on cell-substrate attachment strength.

    abstract::For many cell types, growth, differentiation, and motility are dependent on receptor-mediated adhesion to ligand-coated surfaces. Focal contacts are strong, specialized, adhesive connections between cell and substrate in which receptors aggregate and connect extracellular ligand to intracellular cytoskeletal molecules...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ward MD,Hammer DA

    更新日期:1993-03-01 00:00:00

  • Force-generating cross-bridges during ramp-shaped releases: evidence for a new structural state.

    abstract::Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

    更新日期:2009-02-18 00:00:00

  • Information-theoretic significance of Gibbs energy supply to editing mechanisms.

    abstract::If a branched multistep editing mechanism is implemented by an enzyme with a single site for the specific substrates, there is no reason to believe that the number of testing steps is fixed and cannot be controlled by some external factors. The paper considers the mechanisms of single- or multistep editing done in res...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kremen A

    更新日期:1982-11-01 00:00:00

  • Signal Destruction Tunes the Zone of Activation in Spatially Distributed Signaling Networks.

    abstract::Diverse microbial communities coordinate group behaviors through signal exchange, such as the exchange of acyl-homoserine lactones (AHLs) by Gram-negative bacteria. Cellular communication is prone to interference by neighboring microbes. One mechanism of interference is signal destruction through the production of an ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Silva KP,Chellamuthu P,Boedicker JQ

    更新日期:2017-03-14 00:00:00

  • Four-alpha-helix bundle with designed anesthetic binding pockets. Part I: structural and dynamical analyses.

    abstract::The four-alpha-helix bundle mimics the transmembrane domain of the Cys-loop receptor family believed to be the protein target for general anesthetics. Using high resolution NMR, we solved the structure (Protein Data Bank ID: 2I7U) of a prototypical dimeric four-alpha-helix bundle, (Aalpha(2)-L1M/L38M)(2,) with designe...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ma D,Brandon NR,Cui T,Bondarenko V,Canlas C,Johansson JS,Tang P,Xu Y

    更新日期:2008-06-01 00:00:00

  • The effect of thin filament activation on the attachment of weak binding cross-bridges: A two-dimensional x-ray diffraction study on single muscle fibers.

    abstract::To study possible structural changes in weak cross-bridge attachment to actin upon activation of the thin filament, two-dimensional (2D) x-ray diffraction patterns of skinned fibers from rabbit psoas muscle were recorded at low and high calcium concentration in the presence of saturating concentrations of MgATPgammaS,...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kraft T,Xu S,Brenner B,Yu LC

    更新日期:1999-03-01 00:00:00