Spontaneous-curvature theory of clathrin-coated membranes.

Abstract:

:Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuser (J. Cell Biol. 108:401-411) that suggest the transformation proceeds by changes in the chemical environment of the clathrin lattice, wherein the chemical environment determines the amount of intrinsic, or spontaneous, curvature of the network. We show that a necessary step of the transformation, formation of free pentagons in the clathrin network, can proceed via dislocation unbinding, driven by changes in the spontaneous curvature. Dislocation unbinding is shown to favor formation of coated vesicles that are quite small compared to those predicted by the current continuum theories, which do not include the topology of the clathrin lattice.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Mashl RJ,Bruinsma RF

doi

10.1016/S0006-3495(98)77993-7

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

2862-75

issue

6

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(98)77993-7

journal_volume

74

pub_type

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