Vesicle size distributions measured by flow field-flow fractionation coupled with multiangle light scattering.

abstract：:The effect of a transmembrane peptide on the domain structure of a two-component, two-phase lipid bilayer composed of dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) was examined by spin label electron spin resonance (ESR) spectroscopy. The peptide, pOmpA, is the hydrophobic, 25-residu...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Gierasch LM,Thompson TE

更新日期：1994-06-01 00:00:00

abstract：:The hydrodynamic properties of rigid particles are calculated from models composed of spherical elements (beads) using theories developed by Kirkwood, Bloomfield, and their coworkers. Bead models have usually been built in such a way that the beads fill the volume occupied by the particles. Sometimes the beads are few...

journal_title：Biophysical journal

authors： Carrasco B,García de la Torre J

更新日期：1999-06-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...

journal_title：Biophysical journal

authors： Lakatos M,Groma GI,Ganea C,Lanyi JK,Váró G

更新日期：2002-04-01 00:00:00

abstract：:Investigating the behavior of breast cancer cells via reaction kinetics may help unravel the mechanisms that underlie metabolic changes in tumors. However, obtaining human in vivo kinetic data is challenging because of difficulties associated with measuring these parameters. Nondestructive methods of measuring lipid c...

journal_title：Biophysical journal

authors： Hou J,Reid NE,Tromberg BJ,Potma EO

更新日期：2020-07-21 00:00:00

abstract：:The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

journal_title：Biophysical journal

authors： Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

更新日期：2009-01-01 00:00:00

abstract：:Results are presented of nonphotochemical-hole-burning experiments on the mitochondrial specific dye rhodamine 800 incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV167 carcinoma cells. This dye is selective for the plasma and inner membranes of the mitochondria, as shown b...

journal_title：Biophysical journal

authors： Walsh RJ,Reinot T,Hayes JM,Kalli KR,Hartmann LC,Small GJ

更新日期：2003-02-01 00:00:00

abstract：:Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

journal_title：Biophysical journal

authors： Sun C,Munn LL

更新日期：2005-03-01 00:00:00

abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

journal_title：Biophysical journal

authors： Schwartz TL,Kado RT

更新日期：1977-06-01 00:00:00

abstract：:Dispersed pump-dump-probe spectroscopy has the ability to characterize and identify the underlying ultrafast dynamical processes in complicated chemical and biological systems. This technique builds on traditional pump-probe techniques by exploring both ground- and excited-state dynamics and characterizing the connect...

journal_title：Biophysical journal

authors： Larsen DS,Vengris M,van Stokkum IH,van der Horst MA,de Weerd FL,Hellingwerf KJ,van Grondelle R

更新日期：2004-04-01 00:00:00

abstract：:We perform single-molecule flow experiments using confocal microscopy and a microfluidic device for shear rates up to 20,000 s-1 and present results for the shear-induced unraveling and elongation of tethered von Willebrand factor (VWF) multimers. Further, we employ companion Brownian dynamics simulations to help expl...

journal_title：Biophysical journal

authors： Wang Y,Morabito M,Zhang XF,Webb E 3rd,Oztekin A,Cheng X

更新日期：2019-06-04 00:00:00

abstract：:Bipolar tetraether lipids (BTLs) are abundant in crenarchaeota, which thrive in both thermophilic and nonthermophilic environments, with wide-ranging growth temperatures (4-108°C). BTL liposomes can serve as membrane models to explore the role of BTLs in the thermal stability of the plasma membrane of crenarchaeota. I...

journal_title：Biophysical journal

authors： Chong PL,Sulc M,Winter R

更新日期：2010-11-17 00:00:00

abstract：:The relation between the dynamic properties of the haptenic site of lipid haptens and the phase transition of the host lattice was investigated using head group spin-labeled phosphatidylethanolamines, that is, spin-label lipid haptens (Brûlet, P., and H. M. McConnell, 1976, Proc. Natl. Acad. Sci. USA., 73:2977-2981; B...

journal_title：Biophysical journal

authors： Takeshita K,Utsumi H,Hamada A

更新日期：1987-08-01 00:00:00

abstract：:A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including (13)C and (15)N chemical shift anisotropies and (13)C-(15)N dipolar coupl...

journal_title：Biophysical journal

authors： Holt A,Rougier L,Réat V,Jolibois F,Saurel O,Czaplicki J,Killian JA,Milon A

更新日期：2010-05-19 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:Ubiquinol cytochrome c oxidoreductase (bc1 complex) serves as an important electron junction in many respiratory systems. It funnels electrons coming from NADH and ubiquinol to cytochrome c, but it is also capable of producing significant amounts of the free radical superoxide. In situ and in other experimental system...

journal_title：Biophysical journal

authors： Bazil JN

更新日期：2017-10-03 00:00:00

abstract：:A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the sc...

journal_title：Biophysical journal

authors： Anderson TG,McConnell HM

更新日期：2001-11-01 00:00:00

abstract：:We present a theoretical and experimental analysis of the effects of nonlinear flow in a cone-plate viscometer. The analysis predicts that flow in the viscometer is a function of two parameters, the Reynolds number and the cone angle. Nonlinear flow occurs at high shear rates and causes spatial variations in wall shea...

journal_title：Biophysical journal

authors： Shankaran H,Neelamegham S

更新日期：2001-06-01 00:00:00

abstract：:The single photon response in vertebrate phototransduction is highly reproducible despite a number of random components of the activation cascade, including the random activation site, the random walk of an activated receptor, and its quenching in a random number of steps. Here we use a previously generated and tested...

journal_title：Biophysical journal

authors： Bisegna P,Caruso G,Andreucci D,Shen L,Gurevich VV,Hamm HE,DiBenedetto E

更新日期：2008-05-01 00:00:00

abstract：:Forces generated by microtubule polymerization and depolymerization are important for the biological functioning of cells. The mean growth velocity, V, under an opposing force, F, has been measured by; Science 278:856-860) for single microtubules growing in vitro, but their analysis of the data suggested that V decrea...

journal_title：Biophysical journal

authors： Kolomeisky AB,Fisher ME

更新日期：2001-01-01 00:00:00

abstract：:Current viral docking models have relied upon the assumption that bond formation and breakage are independent of viral and docking surface geometry, as well as the forces exerted on the bonds. This assumption, known as the equivalent site hypothesis (ESH), is examined in detail using a newly developed simulation techn...

journal_title：Biophysical journal

authors： English TJ,Hammer DA

更新日期：2004-06-01 00:00:00

abstract：:The aggregation of amyloid-β (Aβ) peptides plays a crucial role in the etiology of Alzheimer's disease (AD). Recently, it has been reported that an A2T mutation in Aβ can protect against AD. Interestingly, a nonpolar A2V mutation also has been found to offer protection against AD in the heterozygous state, although it...

journal_title：Biophysical journal

authors： Das P,Murray B,Belfort G

更新日期：2015-02-03 00:00:00

abstract：:Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the different...

journal_title：Biophysical journal

authors： Ladokhin AS,Selsted ME,White SH

更新日期：1997-04-01 00:00:00

abstract：:Elongation of the mitotic spindle during anaphase B contributes to chromosome segregation in many cells. Here, we quantitatively test the ability of two models for spindle length control to describe the dynamics of anaphase B spindle elongation using experimental data from Drosophila embryos. In the slide-and-flux-or-...

journal_title：Biophysical journal

authors： Brust-Mascher I,Civelekoglu-Scholey G,Scholey JM

更新日期：2015-04-21 00:00:00

abstract：:Cyclic nucleotide-gated (CNG) channels, which were initially studied in retina and olfactory neurons, are activated by cytoplasmic cGMP or cAMP. Detailed comparisons of nucleotide-activated currents using nucleotide analogs and mutagenesis revealed channel-specific residues in the nucleotide-binding domain that regula...

journal_title：Biophysical journal

authors： Scott SP,Cummings J,Joe JC,Tanaka JC

更新日期：2000-05-01 00:00:00

abstract：:Nuclear transport receptors (NTRs) mediate nucleocytoplasmic transport via their affinity for unstructured proteins (polymers) in the nuclear pore complex (NPC). Here, we have modeled the effect of NTRs on polymeric structure in the nanopore confinement of the NPC central conduit. The model explicitly takes into accou...

journal_title：Biophysical journal

authors： Osmanović D,Ford IJ,Hoogenboom BW

更新日期：2013-12-17 00:00:00

abstract：:The binding constants, K(1) and K(2), and the number of Ca(2+) ions in each of the two high affinity sites of Ca(2+)-regenerated bacteriorhodopsin (bR) are determined potentiometrically at different pH values in the range of pH 3.5-4.5 by using the Scatchard plot method. From the pH dependence of K(1) and K(2), it was...

journal_title：Biophysical journal

authors： Zhang YN,Sweetman LL,Awad ES,El-Sayed MA

更新日期：1992-05-01 00:00:00

abstract：:Spin inversion transfer (SIT) NMR experiments are reported probing the thermodynamics and kinetics of interconversion of two folded forms of a GCN4-like leucine zipper near room temperature. The peptide is 13Calpha-labeled at position V9(a) and results are compared with prior findings for position L13(e). The SIT data...

journal_title：Biophysical journal

authors： d'Avignon DA,Bretthorst GL,Holtzer ME,Holtzer A

更新日期：1999-05-01 00:00:00

abstract：:Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by dete...

journal_title：Biophysical journal

authors： Carattino A,Keizer VIP,Schaaf MJM,Orrit M

更新日期：2016-12-06 00:00:00

abstract：:The state of photosystem II core complex (PS II CC) in monolayer at the gas-water interface was investigated using in situ polarization-modulated infrared reflection absorption spectroscopy and x-ray reflectivity techniques. Two approaches for preparing and manipulating the monolayers were examined and compared. In th...

journal_title：Biophysical journal

authors： Gallant J,Desbat B,Vaknin D,Salesse C

更新日期：1998-12-01 00:00:00