Abstract:
:The effect of S-methylating cysteine-102 (cys-102) (SH----SSCH3) of yeast isozyme-1 (iso-1) ferricytochrome c has been studied using proton NMR spectroscopy. COSY, NOESY, and one-dimensional nuclear Overhauser effect (NOE) difference spectroscopies have all been used. The NMR spectrum of this derivative is very similar to that of native yeast iso-1 ferricytochrome c. The advantage of using the cys-102 S-methylated derivative is that it is unable to spontaneously dimerize in solution, like native iso-1 monomer does. This makes the derivative a simple, ideal protein for long NMR experiments. This work yields many proton resonance assignments for S-methylated yeast iso-1 monomer and confirms all of the assignments for iso-1 monomer that were previously made using only the one-dimensional NOE method.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Busse SC,Moench SJ,Satterlee JDdoi
10.1016/S0006-3495(90)82352-3subject
Has Abstractpub_date
1990-07-01 00:00:00pages
45-51issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(90)82352-3journal_volume
58pub_type
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