Abstract:
:We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing outer leaflet decreased, whereas that in the DOPC-containing inner leaflet was largely unchanged, confirming asymmetry and a lack of coupling between the physical states of the inner and outer leaflets. In contrast, after bSM was exchanged into brain phosphatidylcholine vesicles, lateral diffusion decreased in both leaflets. Thus, asymmetric GUVs should be useful for investigating the molecular mechanisms behind interleaflet coupling.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Chiantia S,Schwille P,Klymchenko AS,London Edoi
10.1016/j.bpj.2010.11.051subject
Has Abstractpub_date
2011-01-05 00:00:00pages
L1-3issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(10)01478-5journal_volume
100pub_type
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