A Thermodynamic Model for Genome Packaging in Hepatitis B Virus.

abstract：:Perfusion is one of the most important processes maintaining organ health. From a computational perspective, however, perfusion is among the least-studied physiological processes of the heart. The recent development of novel nanoparticle-based targeted cardiac therapy calls for novel simulation methods that can provid...

journal_title：Biophysical journal

authors： Diem AK,Valen-Sendstad K

更新日期：2019-12-17 00:00:00

abstract：:A new flow configuration allows a flow cytometer of high resolution and stability to be assembled from an inverted fluorescence microscope with incident illumination, a pulse photometer, and a multichannel pulse height analyzer. A nozzle produces a hydrodynamically focused sample stream in a liquid jet that id directe...

journal_title：Biophysical journal

authors： Lindmo T,Steen HB

更新日期：1979-10-01 00:00:00

abstract：:The origin of Ibetanull, the Ca2+ current of myotubes from mice lacking the skeletal dihydropyridine receptor (DHPR) beta1a subunit, was investigated. The density of Ibetanull was similar to that of Idys, the Ca2+ current of myotubes from dysgenic mice lacking the skeletal DHPR alpha1S subunit (-0.6 +/- 0.1 and -0.7 +...

journal_title：Biophysical journal

authors： Strube C,Beurg M,Sukhareva M,Ahern CA,Powell JA,Powers PA,Gregg RG,Coronado R

更新日期：1998-07-01 00:00:00

abstract：:Calmodulin (CaM) expression is upregulated upon HIV-1 infection and interacts with proteins involved in viral processing, including the multifunctional HIV-1 MA protein. We present here the results of studies utilizing small-angle neutron scattering with contrast variation that, when considered in the light of earlier...

journal_title：Biophysical journal

authors： Taylor JE,Chow JYH,Jeffries CM,Kwan AH,Duff AP,Hamilton WA,Trewhella J

更新日期：2012-08-08 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eule...

journal_title：Biophysical journal

authors： Simon SI,Schmid-Schönbein GW

更新日期：1990-08-01 00:00:00

abstract：:Despite the spontaneity of some in vitro protein-folding reactions, native folding in vivo often requires the participation of barrel-shaped multimeric complexes known as chaperonins. Although it has long been known that chaperonin substrates fold upon sequestration inside the chaperonin barrel, the precise mechanism ...

journal_title：Biophysical journal

authors： England JL,Pande VS

更新日期：2008-10-01 00:00:00

abstract：:The sequence of events involved in the transition from attached liposomes to bilayer patches on hydrophilic and hydrophobic solid supports were visualized in situ by Tapping Mode atomic force microscopy in liquid. In a smooth manner, the attached liposomes spread and flattened from the outer edges toward the center un...

journal_title：Biophysical journal

authors： Jass J,Tjärnhage T,Puu G

更新日期：2000-12-01 00:00:00

abstract：:To investigate the mechanism of interaction of gramicidin S-like antimicrobial peptides with biological membranes, a series of five decameric cyclic cationic beta-sheet-beta-turn peptides with all possible combinations of aromatic D-amino acids, Cyclo(Val-Lys-Leu-D-Ar1-Pro-Val-Lys-Leu-D-Ar2-Pro) (Ar identical with Phe...

journal_title：Biophysical journal

authors： Jelokhani-Niaraki M,Hodges RS,Meissner JE,Hassenstein UE,Wheaton L

更新日期：2008-10-01 00:00:00

abstract：:39K nuclear magnetic resonance (NMR) spectra were readily obtained, in vivo, from rat muscle, kidney, and brain in 5-10 min with signal-to-noise ratios of approximately 20:1. Quantitation of the K+ signal was achieved by reference to an external standard of KCl/dysprosium nitrate as well as by reference to the proton ...

journal_title：Biophysical journal

authors： Adam WR,Koretsky AP,Weiner MW

更新日期：1987-02-01 00:00:00

abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...

journal_title：Biophysical journal

authors： Shih AY,Sligar SG,Schulten K

更新日期：2008-06-01 00:00:00

abstract：:The shapes of fluorescence induction curves in spinach chloroplasts, measured using double-flash pump-probe techniques, are shown to depend on the duration of the actinic flashes. For flash durations tau(0)

journal_title：Biophysical journal

authors： Valkunas L,Geacintov NE,France L,Breton J

更新日期：1991-02-01 00:00:00

abstract：:The dynamics of protein distribution in endocytic membranes are relevant for many cellular processes, such as protein sorting, organelle and membrane microdomain biogenesis, protein-protein interactions, receptor function, and signal transduction. We have developed an assay based on Fluorescence Resonance Energy Micro...

journal_title：Biophysical journal

authors： Wallrabe H,Elangovan M,Burchard A,Periasamy A,Barroso M

更新日期：2003-07-01 00:00:00

abstract：:Certain antibiotic peptides are thought to permeabilize membranes of pathogens by effects that are also observed for simple detergents, such as membrane thinning and disordering, asymmetric bilayer expansion, toroidal pore formation, and micellization. Here we test the hypothesis that such peptides act additively with...

journal_title：Biophysical journal

authors： Patel H,Huynh Q,Bärlehner D,Heerklotz H

更新日期：2014-05-20 00:00:00

abstract：:In the rabbit corneal stroma, the swelling pressure, P, has been reported to have an anomalous (negative) temperature coefficient, alpha P, contradicting traditional Donnan swelling theory. A parallel-plate, diffuse double layer Gouy-Chapman model was used to resolve this discrepancy. The present model incorporates th...

journal_title：Biophysical journal

authors： Kwok LS,Klyce SD

更新日期：1990-03-01 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of t...

journal_title：Biophysical journal

authors： Zahler R,Bittl JA,Ingwall JS

更新日期：1987-06-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:In recent experimental reports, robust circadian oscillation of the phosphorylation level of KaiC has been reconstituted by incubating three cyanobacterial proteins, KaiA, KaiB, and KaiC, with ATP in vitro. This reconstitution indicates that protein-protein interactions and the associated ATP hydrolysis suffice to gen...

journal_title：Biophysical journal

authors： Nagai T,Terada TP,Sasai M

更新日期：2010-06-02 00:00:00

abstract：:Interactions between the graft copolymer poly(L-lysine)-g-poly(ethylene glycol), PLL-g-PEG, and two kinds of surface-supported lipidic systems (supported phospholipid bilayers and supported vesicular layers) were investigated by a combination of microscopic and spectroscopic techniques. It was found that the applicati...

journal_title：Biophysical journal

authors： Rossetti FF,Reviakine I,Csúcs G,Assi F,Vörös J,Textor M

更新日期：2004-09-01 00:00:00

abstract：:Despite the enormous interest that has been devoted to the study of farnesyltransferase, many questions concerning its catalytic mechanism remain unanswered. In particular, several doubts exist on the structure of the active-site zinc coordination sphere, more precisely on the nature of the fourth ligand, which is dis...

journal_title：Biophysical journal

authors： Sousa SF,Fernandes PA,Ramos MJ

更新日期：2005-01-01 00:00:00

abstract：:The photoelectron quantum yields of 21 common amino acids and 15 polyamino acids were measured in the 180-240 nm wavelength region. On the average, the quantum yields of these two groups exhibit quite similar wavelength dependence. For lambda > 220 nm all amino acid and polyamino acid quantum yields are

journal_title：Biophysical journal

authors： Dam RJ,Burke CA,Griffith OH

更新日期：1974-06-01 00:00:00

abstract：:A structural model is suggested for axon membranes consisting of a double layer of lipid and phospholipid molecules in which the polar ends of certain phospholipids change their orientation and combining properties under the influence of an electric field. The phosphate groups act as ion exchange "gates" for the contr...

journal_title：Biophysical journal

authors： GOLDMANN DE

更新日期：1964-05-01 00:00:00

abstract：:In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which ...

journal_title：Biophysical journal

authors： Colloc'h N,Sopkova-de Oliveira Santos J,Retailleau P,Vivarès D,Bonneté F,Langlois d'Estainto B,Gallois B,Brisson A,Risso JJ,Lemaire M,Prangé T,Abraini JH

更新日期：2007-01-01 00:00:00

abstract：:The lateral mobility of the lipid analog N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine and of the integral protein glycophorin in giant dimyristoylphosphatidylcholine vesicles was studied by the photobleaching technique. Above the temperature of the chain-melting transition (Tm = 23 degrees C), the diffusi...

journal_title：Biophysical journal

authors： Kapitza HG,Rüppel DA,Galla HJ,Sackmann E

更新日期：1984-03-01 00:00:00

abstract：:Eukaryotic DNA is packaged into the cell nucleus as a nucleoprotein complex, chromatin. Despite this condensed state, access to the DNA sequence must occur during gene expression and other essential genetic events. Here we employ optical tweezers stretching of reconstituted chromatin fibers to investigate the release ...

journal_title：Biophysical journal

authors： Pope LH,Bennink ML,van Leijenhorst-Groener KA,Nikova D,Greve J,Marko JF

更新日期：2005-05-01 00:00:00

abstract：:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting...

journal_title：Biophysical journal

authors： Olesen SP,Crone C

更新日期：1983-04-01 00:00:00

abstract：:Why is the spine of a neuron so small that it can contain only small numbers of molecules and reactions inevitably become stochastic? We previously showed that, despite such noisy conditions, the spine exhibits robust, sensitive, and efficient features of information transfer using the probability of Ca2+ increase; ho...

journal_title：Biophysical journal

authors： Fujii M,Ohashi K,Karasawa Y,Hikichi M,Kuroda S

更新日期：2017-02-28 00:00:00

abstract：:A dynamic model of smooth muscle contraction is presented and is compared with the mechanical properties of vascular smooth muscle in the rat portal vein. The model is based on the sliding filament theory and the assumption that force is produced by cross-bridges extending from the myosin to the actin filaments. Thus,...

journal_title：Biophysical journal

authors： Gestrelius S,Borgström P

更新日期：1986-07-01 00:00:00