Abstract:
:Changes in the ultraviolet light scattering from a suspension of purple membrane fragments were detected during the photocycle of bacteriorhodopsin with a cross-correlation method. The scattered light intensity from a suspension of membrane fragments containing the protein bacteriorhodopsin was measured on a logarithmic time scale of 1 microsecond to 0.1 s at pH 4.6 after the photocycle was initiated with a polarized 532-nm laser flash. A simple model of curved sheets with positive and negative changes in the curvature is used to describe the observed light scattering changes. A detailed mathematical derivation of the model as well as the pictorial description are given. The changes in curvature of the membrane fragment are more than likely driven by the protein during the photocycle and are observed to have at least two time-resolved components, each changing the curvature of the fragment with an opposite sign.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Czégé J,Reinisch Ldoi
10.1016/S0006-3495(90)82415-2subject
Has Abstractpub_date
1990-09-01 00:00:00pages
721-9issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(90)82415-2journal_volume
58pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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