Short peptide amyloid organization: stabilities and conformations of the islet amyloid peptide NFGAIL.

abstract：:A 15-ns molecular dynamics (MD) simulation of the fully hydrated dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer in the liquid-crystalline state was carried out to investigate the effect of Chol on the hydrocarbon chain region of the bilayer. The last 8-ns fragment of the generated trajectory was used f...

journal_title：Biophysical journal

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-10-01 00:00:00

abstract：:We study a Brownian dynamics simulation model of a biopolymeric shell deformed by axial forces exerted at opposing poles. The model exhibits two distinct, linear force-extension regimes, with the response to small tensions governed by linear elasticity and the response to large tensions governed by an effective spring...

journal_title：Biophysical journal

authors： Banigan EJ,Stephens AD,Marko JF

更新日期：2017-10-17 00:00:00

abstract：:A new flow configuration allows a flow cytometer of high resolution and stability to be assembled from an inverted fluorescence microscope with incident illumination, a pulse photometer, and a multichannel pulse height analyzer. A nozzle produces a hydrodynamically focused sample stream in a liquid jet that id directe...

journal_title：Biophysical journal

authors： Lindmo T,Steen HB

更新日期：1979-10-01 00:00:00

abstract：:Changes in voltage-dependent gating represent a common pathogenetic mechanism for genetically inherited channelopathies, such as benign familial neonatal seizures or peripheral nerve hyperexcitability caused by mutations in neuronal K(v)7.2 channels. Mutation-induced changes in channel voltage dependence are most ofte...

journal_title：Biophysical journal

authors： Miceli F,Vargas E,Bezanilla F,Taglialatela M

更新日期：2012-03-21 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study molecular dynamics inside living cells. During the past years, several laboratories have used FRAP to image the motion of RNA-protein and other macromolecular complexes in the nucleus and cytoplasm. In the case of mRNAs, there is growin...

journal_title：Biophysical journal

authors： Braga J,McNally JG,Carmo-Fonseca M

更新日期：2007-04-15 00:00:00

abstract：:Most of the decisive molecular events in biology take place at the protein-water interface. The dynamical properties of the hydration layer are therefore of fundamental importance. To characterize the dynamical heterogeneity and rotational activation energy in the hydration layer, we measured the (17)O spin relaxation...

journal_title：Biophysical journal

authors： Mattea C,Qvist J,Halle B

更新日期：2008-09-15 00:00:00

abstract：:Molecular dynamics simulations of protein tyrosine phosphatase 1B (PTP1B) complexed with the phosphorylated peptide substrate DADEpYL and the free substrate have been conducted to investigate 1) the physical forces involved in substrate-protein interactions, 2) the importance of enzyme and substrate flexibility for bi...

journal_title：Biophysical journal

authors： Peters GH,Frimurer TM,Andersen JN,Olsen OH

更新日期：2000-05-01 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:Voltage-gated ion channels are crucial for regulation of electric activity of excitable tissues such as nerve cells, and play important roles in many diseases. During activation, the charged S4 segment in the voltage sensor domain translates across a hydrophobic core forming a barrier for the gating charges. This barr...

journal_title：Biophysical journal

authors： Schwaiger CS,Liin SI,Elinder F,Lindahl E

更新日期：2013-01-08 00:00:00

abstract：:A method for combining calculations of residue pKa's with changes in the position of polar hydrogens has been developed. The Boltzmann distributions of proton positions in hydroxyls and neutral titratable residues are found in the same Monte Carlo sampling procedure that determines the amino acid ionization states at ...

journal_title：Biophysical journal

authors： Alexov EG,Gunner MR

更新日期：1997-05-01 00:00:00

abstract：:Sinusoidal oscillatory flow of blood and of aqueous glycerol solutions was produced in rigid cylindrical tubes. For aqueous glycerol, the amplitude of the measured pressure gradient wave form conformed closely to that predicted by Womersley's theory of oscillatory flow, up to Reynolds numbers approaching 2000. Blood d...

journal_title：Biophysical journal

authors： Kunz AL,Coulter NA Jr

更新日期：1967-01-01 00:00:00

abstract：:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

journal_title：Biophysical journal

authors： Dusenbery DB,Uretz RB

更新日期：1972-08-01 00:00:00

abstract：:Electrical bursting oscillations of mammalian pancreatic beta-cells are synchronous among cells within an islet. While electrical coupling among cells via gap junctions has been demonstrated, its extent and topology are unclear. The beta-cells also share an extracellular compartment in which oscillations of K+ concent...

journal_title：Biophysical journal

authors： Stokes CL,Rinzel J

更新日期：1993-08-01 00:00:00

abstract：:Recent studies suggest that phosphoinositide kinases may participate in intracellular trafficking or exocytotic events. Because both of these events ultimately require fusion of biological membranes, the susceptibility of membranes containing polyphosphoinositides (PPIs) to divalent cation-induced fusion was investiga...

journal_title：Biophysical journal

authors： Summers SA,Guebert BA,Shanahan MF

更新日期：1996-12-01 00:00:00

abstract：:We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicl...

journal_title：Biophysical journal

authors： Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XL

更新日期：2005-11-01 00:00:00

abstract：:Hundreds of cationic antimicrobial and cell-penetrating peptides (CPPs) form amphipathic α-helices when bound to lipid membranes. Here, we test two hypotheses for the differences in the ability of these peptides to translocate across membranes. The first, which we now call the hydrophobicity hypothesis, is that peptid...

journal_title：Biophysical journal

authors： Ablan FDO,Spaller BL,Abdo KI,Almeida PF

更新日期：2016-10-18 00:00:00

abstract：:We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate the applicability of ...

journal_title：Biophysical journal

authors： Meller A,Bar-Ziv R,Tlusty T,Moses E,Stavans J,Safran SA

更新日期：1998-03-01 00:00:00

abstract：:The selective permeability to monovalent metal cations, as well as the relationship between cation permeation and gating kinetics, was investigated for native tetrodotoxin-insensitive Na-channels in guinea pig ventricular myocytes using the whole-cell patch clamp technique. By the measurement of inward unidirectional ...

journal_title：Biophysical journal

authors： Kurata Y,Sato R,Hisatome I,Imanishi S

更新日期：1999-10-01 00:00:00

abstract：:We have measured, using infrared spectroscopy, the hydrogen/deuterium exchange rates of the amide protons in the photosynthetic antenna of Rhodospirillum rubrum. These measurements were made not only on the intact protein in detergent solution but also on two dissociated forms (B820 and B777). We have, on the basis of...

journal_title：Biophysical journal

authors： Sturgis J,Robert B,Goormaghtigh E

更新日期：1998-02-01 00:00:00

abstract：:Interactions between proteins are often sufficiently weak that their study through the use of conventional structural techniques becomes problematic. Of the few techniques capable of providing experimental measures of weak protein-protein interactions, perhaps the most useful is the second virial coefficient, B(22), w...

journal_title：Biophysical journal

authors： Elcock AH,McCammon JA

更新日期：2001-02-01 00:00:00

abstract：:We perform single-molecule flow experiments using confocal microscopy and a microfluidic device for shear rates up to 20,000 s-1 and present results for the shear-induced unraveling and elongation of tethered von Willebrand factor (VWF) multimers. Further, we employ companion Brownian dynamics simulations to help expl...

journal_title：Biophysical journal

authors： Wang Y,Morabito M,Zhang XF,Webb E 3rd,Oztekin A,Cheng X

更新日期：2019-06-04 00:00:00

abstract：:Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding...

journal_title：Biophysical journal

authors： Salerno D,Beretta GL,Zanchetta G,Brioschi S,Cristofalo M,Missana N,Nardo L,Cassina V,Tempestini A,Giovannoni R,Cerrito MG,Zaffaroni N,Bellini T,Mantegazza F

更新日期：2016-05-24 00:00:00

abstract：:We have interrogated the isothermal folding behavior of the DNA i-motif of the human telomere, dC19, and a high-stability i-motif-forming sequence in the promoter of the human DNA repair gene RAD17 using human physiological solution and temperature conditions. We developed a circular-dichroism-spectroscopy-based pH ti...

journal_title：Biophysical journal

authors： Rogers RA,Fleming AM,Burrows CJ

更新日期：2018-04-24 00:00:00

abstract：:To determine if hydrophobic surfactant proteins affect the stability of pulmonary surfactant monolayers at an air/water interface, the studies reported here compared the kinetics of collapse for the complete set of lipids in calf surfactant with and without the proteins. Monomolecular films spread at the surface of ca...

journal_title：Biophysical journal

authors： Lhert F,Yan W,Biswas SC,Hall SB

更新日期：2007-12-15 00:00:00

abstract：:In most environments, such as natural aquatic systems, bacteria are found predominantly in self-organized sessile communities known as biofilms. In the presence of a significant flow, mature multispecies biofilms often develop into long filamentous structures called streamers, which can greatly influence ecosystem pro...

journal_title：Biophysical journal

authors： Rusconi R,Lecuyer S,Autrusson N,Guglielmini L,Stone HA

更新日期：2011-03-16 00:00:00

abstract：:We describe a statistical technique, the joint peristimulus time (PST) scatter diagram, for the analysis of data from simultaneously recorded neurons subjected to repeated stimulation. Distinguishable features in the scatter diagram are related to effects of the stimulus on the observed neurons and to functional relat...

journal_title：Biophysical journal

authors： Gerstein GL,Perkel DH

更新日期：1972-05-01 00:00:00

abstract：:We have applied two-dimensional infrared (2D IR) and betanu correlation spectroscopy to in-situ IR spectroscopy of pulmonary surfactant proteins SP-B and SP-C in lipid-protein monolayers at the air-water interface. For both SP-B and SP-C, a statistical windowed autocorrelation method identified two separate surface pr...

journal_title：Biophysical journal

authors： Shanmukh S,Howell P,Baatz JE,Dluhy RA

更新日期：2002-10-01 00:00:00

abstract：:Cell spreading is involved in many physiological and pathological processes. The spreading behavior of a cell significantly depends on its microenvironment, but the biochemomechanical mechanisms of geometry-confined cell spreading remain unclear. A dynamic model is here established to investigate the spreading of cell...

journal_title：Biophysical journal

authors： Zhao ZL,Liu ZY,Du J,Xu GK,Feng XQ

更新日期：2017-06-06 00:00:00

abstract：:The intracellular and interstitial potentials associated with each cell or fiber in multicellular preparations carrying a uniformly propagating wave are important for characterizing the electrophysiological behavior of the preparation and in particular, for evaluating the source contributed by each fiber. The aforemen...

journal_title：Biophysical journal

authors： Henriquez CS,Trayanova N,Plonsey R

更新日期：1988-06-01 00:00:00

abstract：:We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays...

journal_title：Biophysical journal

authors： Guáqueta C,Sanders LK,Wong GC,Luijten E

更新日期：2006-06-15 00:00:00