Diffusion of extracellular K+ can synchronize bursting oscillations in a model islet of Langerhans.

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:An equation of state for lipid monolayers at the air-water interface is presented, which takes into account the effects of the conformation and the number and position of double bonds of the hydrocarbon chains. The total Hamiltonian of the monolayer is assumed to consist of three terms. Two of them are calculated exac...

journal_title：Biophysical journal

authors： Feng SS,MacDonald RC

更新日期：1995-08-01 00:00:00

abstract：:The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal...

journal_title：Biophysical journal

authors： Bullen A,Patel SS,Saggau P

更新日期：1997-07-01 00:00:00

abstract：:The effects of low pH, and of alkaline earth cations, were examined on calcium uptake by pinched-off nerve terminals (synaptosomes). This uptake appears to be mediated by voltage-sensitive Ca channels (J. Physiol. 247:617, 1975). Ca uptake was measured in low (5 mM) or high (77 mM) potassium media. The extra uptake pr...

journal_title：Biophysical journal

authors： Nachshen DA,Blaustein MP

更新日期：1979-05-01 00:00:00

abstract：:This work presents a methodology to measure and quantitatively interpret force curves on supported lipid bilayers in water. We then use this method to correlate topographic imaging contrast in atomic force microscopy (AFM) images of phase-separated Langmuir-Blodgett bilayers with imaging load. Force curves collected o...

journal_title：Biophysical journal

authors： Schneider J,Dufrêne YF,Barger WR Jr,Lee GU

更新日期：2000-08-01 00:00:00

abstract：:The dependence of the fluorescence of catalase upon the concentration of added superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The affinity of SOD for these sites varies with temperature, and with the concentration of each of three nominally inert polymeric additives--dextran 70, Fi...

journal_title：Biophysical journal

authors： Jiao M,Li HT,Chen J,Minton AP,Liang Y

更新日期：2010-08-04 00:00:00

abstract：:The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanis...

journal_title：Biophysical journal

authors： Pfeuty B,Thommen Q,Lefranc M

更新日期：2011-06-08 00:00:00

abstract：:The effect of agarose on nucleation of hen egg white lysozyme crystal was examined quantitatively using a temperature-jumping technique. For the first time, to our knowledge, the inhibition of agarose during the nucleation of lysozyme was quantified in two respects: a), the effect of increasing interfacial nucleation ...

journal_title：Biophysical journal

authors： Wang L,Liu XY

更新日期：2008-12-15 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:F-actin, a helical polymer formed by polymerization of the monomers (G-actin), plays crucial roles in various aspects of cell motility. Flexibility of F-actin has been suggested to be important for such a variety of functions. Understanding the flexibility of F-actin requires characterization of a hierarchy of dynamic...

journal_title：Biophysical journal

authors： Fujiwara S,Plazanet M,Matsumoto F,Oda T

更新日期：2008-06-01 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differi...

journal_title：Biophysical journal

authors： Silvius JR

更新日期：2003-08-01 00:00:00

abstract：:It has been observed in numerical experiments that adding a cargo to a motor protein can regularize its gait. Here we explain these results via asymptotic analysis on a general stochastic motor protein model. This analysis permits a computation of various observables (e.g., the mean velocity) of the motor protein and ...

journal_title：Biophysical journal

authors： DeVille RE,Vanden-Eijnden E

更新日期：2008-09-15 00:00:00

abstract：:The aquaglyceroporin GlpF is a transmembrane channel of Escherichia coli that facilitates the uptake of glycerol by the cell. Its high glycerol uptake rate is crucial for the cell to survive in very low glycerol concentrations. Although GlpF allows both influx and outflux of glycerol, its structure, similar to the str...

journal_title：Biophysical journal

authors： Lu D,Grayson P,Schulten K

更新日期：2003-11-01 00:00:00

abstract：:The study of mechanotransduction relies on tools that are capable of applying mechanical forces to elicit and assess cellular responses. Here we report a new (to our knowledge) technique, called two-bubble acoustic tweezing cytometry (TB-ATC), for generating spatiotemporally controlled subcellular mechanical forces on...

journal_title：Biophysical journal

authors： Chen D,Sun Y,Gudur MS,Hsiao YS,Wu Z,Fu J,Deng CX

更新日期：2015-01-06 00:00:00

abstract：:Formamide-induced detubulation of rat ventricular myocytes was used to investigate the functional distribution of the Na/Ca exchanger (NCX) and Na/K-ATPase between the t-tubules and external sarcolemma. Detubulation resulted in a 32% decrease in cell capacitance, whereas cell volume was unchanged. Thus, the surface-to...

journal_title：Biophysical journal

authors： Despa S,Brette F,Orchard CH,Bers DM

更新日期：2003-11-01 00:00:00

abstract：:Fission fragment tracks from uranium-stained biological preparations have been observed in the electron microscope in a study of the feasibility of their use as tracers. Virus, DNA, and tissue sections were stained with uranium, prepared for electron microscopy, irradiated in the high thermal neutron flux of a nuclear...

journal_title：Biophysical journal

authors： MALMON AG

更新日期：1964-01-01 00:00:00

abstract：:Here we present an efficient implementation of line-scan fluorescence correlation spectroscopy (i.e., one-dimensional spatio-temporal image correlation spectroscopy) using a commercial laser scanning microscope, which allows the accurate measurement of diffusion coefficients and concentrations in biological lipid memb...

journal_title：Biophysical journal

authors： Ries J,Chiantia S,Schwille P

更新日期：2009-03-04 00:00:00

abstract：:The investigation of the mechanism of protein folding is complicated by the context dependence of the rates of intramolecular contact formation. Methods based on site-specific labeling and ultrafast spectroscopic detection of fluorescence signals were developed for monitoring the rates of individual subdomain folding ...

journal_title：Biophysical journal

authors： Rahamim G,Amir D,Haas E

更新日期：2017-05-09 00:00:00

abstract：:Pyranine (8-hydroxy pyrene-1,3,6-trisulfonate) is a commonly used photoacid that discharges a proton when excited to its first electronic singlet state. Follow-up of its dissociation kinetics reveals the physicochemical properties of its most immediate environment. At vanishing ionic strength the dye adsorbs to the Es...

journal_title：Biophysical journal

authors： Nachliel E,Pollak N,Huppert D,Gutman M

更新日期：2001-03-01 00:00:00

abstract：:Cardiac transverse (T)-tubules provide a specialized structure for synchronization and stabilization of sarcoplasmic reticulum Ca(2+) release in healthy cardiomyocytes. The application of laser scanning confocal microscopy and the use of fluorescent lipophilic membrane dyes have boosted the discoveries that T-tubule r...

journal_title：Biophysical journal

authors： Guo A,Song LS

更新日期：2014-06-17 00:00:00

abstract：:If a branched multistep editing mechanism is implemented by an enzyme with a single site for the specific substrates, there is no reason to believe that the number of testing steps is fixed and cannot be controlled by some external factors. The paper considers the mechanisms of single- or multistep editing done in res...

journal_title：Biophysical journal

authors： Kremen A

更新日期：1982-11-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:We have developed a novel, spectroscopic technique for high-sensitivity, label-free DNA quantification. We demonstrate that an optical resonance (whispering gallery mode) excited in a micron-sized silica sphere can be used to detect and measure nucleic acids. The surface of the silica sphere is chemically modified wit...

journal_title：Biophysical journal

authors： Vollmer F,Arnold S,Braun D,Teraoka I,Libchaber A

更新日期：2003-09-01 00:00:00

abstract：:Entropy Sampling Monte Carlo (ESMC) simulations were carried out to study the thermodynamics of the folding transition in the GCN4 leucine zipper (GCN4-lz) in the context of a reduced model. Using the calculated partition functions for the monomer and dimer, and taking into account the equilibrium between the monomer ...

journal_title：Biophysical journal

authors： Mohanty D,Kolinski A,Skolnick J

更新日期：1999-07-01 00:00:00

abstract：:Solid-state NMR study shows that the 22-residue K3 peptide (Ser(20)-Lys(41)) from beta(2)-microglobulin (beta(2)m) adopts a beta-strand-loop-beta-strand conformation in its fibril state. Residue Pro(32) has a trans conformation in the fibril state of the peptide, while it adopts a cis conformation in the native state ...

journal_title：Biophysical journal

authors： Liang C,Derreumaux P,Mousseau N,Wei G

更新日期：2008-07-01 00:00:00

abstract：:More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders...

journal_title：Biophysical journal

authors： Garcia-Mira MM,Sanchez-Ruiz JM

更新日期：2001-12-01 00:00:00

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00

abstract：:Characterizing the ionic distribution around chromatin is important for understanding the electrostatic forces governing chromatin structure and function. Here we develop an electrostatic model to handle multivalent ions and compute the ionic distribution around a mesoscale chromatin model as a function of conformatio...

journal_title：Biophysical journal

authors： Gan HH,Schlick T

更新日期：2010-10-20 00:00:00