Regular gaits and optimal velocities for motor proteins.

abstract：:We report the first time-resolved study of the two-dimensional x-ray diffraction pattern during active contraction in insect flight muscle (IFM). Activation of demembranated Lethocerus IFM was triggered by 1.5-2.5% step stretches (risetime 10 ms; held for 1.5 s) giving delayed active tension that peaked at 100-200 ms....

journal_title：Biophysical journal

authors： Tregear RT,Edwards RJ,Irving TC,Poole KJ,Reedy MC,Schmitz H,Towns-Andrews E,Reedy MK

更新日期：1998-03-01 00:00:00

abstract：:Effects of intracellular ionic strength on the isotonic contraction properties of both intact fibers and skinned fibers give insights into the cross-bridge mechanism, but presently there is fundamental disagreement in the results on the two fiber preparations. This paper, which studies the effects on contraction of va...

journal_title：Biophysical journal

authors： Gulati J,Babu A

更新日期：1984-02-01 00:00:00

abstract：:This contribution describes measurements of lipid bilayer domain line tension based on two-dimensional thermal undulations of membranes with liquid ordered/liquid disordered phase coexistence and near-critical composition at room temperature. Lateral inhomogeneity of lipid and protein composition is currently a subjec...

journal_title：Biophysical journal

authors： Esposito C,Tian A,Melamed S,Johnson C,Tee SY,Baumgart T

更新日期：2007-11-01 00:00:00

abstract：:A filopodium is a cytoplasmic projection, exquisitely built and regulated, which extends from the leading edge of the migrating cell, exploring the cell's neighborhood. Commonly, filopodia grow and retract after their initiation, exhibiting rich dynamical behaviors. We model the growth of a filopodium based on a stoch...

journal_title：Biophysical journal

authors： Lan Y,Papoian GA

更新日期：2008-05-15 00:00:00

abstract：:The widely used hydrophobic cannabinoid ligand CP-55,940 partitions with high efficiency into biomembranes. We studied the location, orientation, and dynamics of CP-55,940 in POPC bilayers by solid-state NMR. Chemical-shift perturbation of POPC protons from the aromatic ring-current effect, as well as 1H NMR cross-rel...

journal_title：Biophysical journal

authors： Kimura T,Cheng K,Rice KC,Gawrisch K

更新日期：2009-06-17 00:00:00

abstract：:Total internal reflection excitation used in combination with fluorescence correlation spectroscopy (TIR-FCS) is a method for characterizing the dynamic behavior and absolute concentrations of fluorescent molecules near or at the interface of a planar substrate and a solution. In this work, we demonstrate for the firs...

journal_title：Biophysical journal

authors： Lieto AM,Cush RC,Thompson NL

更新日期：2003-11-01 00:00:00

abstract：:Coarse-grained molecular dynamics simulations are applied to explore the experimentally observed ability of the liquid-ordered (lo)/liquid-disordered (ld) phase boundary to facilitate viral membrane fusion. Surprisingly, a formed fusion stalk can be both attracted (i.e., stalkophilic) and repelled (i.e., stalkophobic)...

journal_title：Biophysical journal

authors： Risselada HJ

更新日期：2017-06-20 00:00:00

abstract：:The nucleus has a smooth, regular appearance in normal cells, and its shape is greatly altered in human pathologies. Yet, how the cell establishes nuclear shape is not well understood. We imaged the dynamics of nuclear shaping in NIH3T3 fibroblasts. Nuclei translated toward the substratum and began flattening during t...

journal_title：Biophysical journal

authors： Li Y,Lovett D,Zhang Q,Neelam S,Kuchibhotla RA,Zhu R,Gundersen GG,Lele TP,Dickinson RB

更新日期：2015-08-18 00:00:00

abstract：:Molecular chaperones are large proteins or protein complexes from which many proteins require assistance in order to fold. One unique property of molecular chaperones is the cavity they provide in which proteins fold. The interior surface residues which make up the cavities of molecular chaperone complexes from differ...

journal_title：Biophysical journal

authors： White AD,Huang W,Jiang S

更新日期：2012-12-19 00:00:00

abstract：:The interaction of cells in a tissue depends on the nature of the extracellular matrix. The electrical properties of the narrow extracellular space are unknown. Here we consider cell adhesion mediated by extracellular matrix protein on a solid substrate as a model system. We culture human embryonic kidney (HEK293) cel...

journal_title：Biophysical journal

authors： Gleixner R,Fromherz P

更新日期：2006-04-01 00:00:00

abstract：:PROPPINs (β-propellers that bind polyphosphoinositides) are a family of PtdIns3P- and PtdIns(3,5)P2-binding proteins that play an important role in autophagy. We analyzed PROPPIN-membrane binding through isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and molecular dynamics (MD)...

journal_title：Biophysical journal

authors： Busse RA,Scacioc A,Krick R,Pérez-Lara Á,Thumm M,Kühnel K

更新日期：2015-05-05 00:00:00

abstract：:We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain size...

journal_title：Biophysical journal

authors： Camley BA,Esposito C,Baumgart T,Brown FL

更新日期：2010-09-22 00:00:00

abstract：:Given some simple kinetic models of the photocycle of bacteriorhodopsin (bR) and data taken at many wavelengths and under conditions that avoid photoselection and steady-state cycling complications, it is shown how to extract the apparent rate constants and the spectra of the intermediates. Special consideration was g...

journal_title：Biophysical journal

authors： Nagle JF,Parodi LA,Lozier RH

更新日期：1982-05-01 00:00:00

abstract：:Membrane potential of aortic endothelial cells under resting conditions is dominated by inward-rectifier K(+) channels belonging to the Kir 2 family. Regulation of endothelial Kir by membrane cholesterol was studied in bovine aortic endothelial cells by altering the sterol composition of the cell membrane. Our results...

journal_title：Biophysical journal

authors： Romanenko VG,Rothblat GH,Levitan I

更新日期：2002-12-01 00:00:00

abstract：:Iberiotoxin, a toxin purified from the scorpion Buthus tamulus is a 37 amino acid peptide having 68% homology with charybdotoxin. Charybdotoxin blocks large conductance Ca(2+)-activated K+ channels at nanomolar concentrations from the external side only (Miller, C., E. Moczydlowski, R. Latorre, and M. Phillips. 1985. ...

journal_title：Biophysical journal

authors： Candia S,Garcia ML,Latorre R

更新日期：1992-08-01 00:00:00

abstract：:Concentrated solutions of bovine beta-lactoglobulin were studied using osmotic stress and rheological techniques. At pH 6.0 and 8.0, the osmotic pressure was largely independent of NaCl concentration and could be described by a hard sphere equation of state. At pH 5.1, close to the isoelectric point, the osmotic press...

journal_title：Biophysical journal

authors： Parker R,Noel TR,Brownsey GJ,Laos K,Ring SG

更新日期：2005-08-01 00:00:00

abstract：:The rate of muscle force redevelopment after release-restretch protocols has previously been interpreted using a simple two-state cross-bridge cycling model with rate constants for transitions between non-force-bearing and force-bearing states, f, and between force-bearing and non-force-bearing states, g. Changes in t...

journal_title：Biophysical journal

authors： Campbell K

更新日期：1997-01-01 00:00:00

abstract：:A single isolated sinoatrial pacemaker cell presents intrinsic interbeat interval (IBI) variability that is believed to result from the stochastic characteristics of the opening and closing processes of membrane ion channels. To our knowledge, a novel mathematical framework was developed in this work to address the ef...

journal_title：Biophysical journal

authors： Dvir H,Zlochiver S

更新日期：2015-03-10 00:00:00

abstract：:Primary embryonic chick cells have been evaluated on the basis of their capacity to repair photochemical lesions produced in the deoxyribonucleic acid (DNA) by ultraviolet (UV) radiation. The fate of one prominent class of UV photoproducts, cyclobutane pyrimidine dimers, was monitored by an in vitro enzymatic assay. U...

journal_title：Biophysical journal

authors： Paterson MC,Lohman PH,de Weerd-Kastelein EA,Westerveld A

更新日期：1974-06-01 00:00:00

abstract：:Effects of alkali cations on the nuclear magnetic resonance intensity of 23Na were studied in rat liver homogenate. The loss in the resonance intensity of 23Na in the homogenate was able to be divided into two components, one of which is abolished by the addition of Cs+ ("Cs-sensitive component"), the other being inse...

journal_title：Biophysical journal

authors： Monoi H

更新日期：1976-12-01 00:00:00

abstract：:An experimental procedure is described which consists of cutting the canal duct, inserting a micropipette and administering known volumetric displacements to the cupula. The cupula is made visible by dying the endolymph. Known displacements are administered to the cupula, and the time constant of the return to its equ...

journal_title：Biophysical journal

authors： Grant JW,Van Buskirk WC

更新日期：1976-06-01 00:00:00

abstract：:Three features appear to characterize steady-state light adaptation in vertebrate cone photoreceptors: (a) the shape of the "log intensity-response" curve at different levels of adaptation is the same, the only change with adaptation is in the position of the point on the curve about which the cones operate; (b) at hi...

journal_title：Biophysical journal

authors： Dawis SM,Purple RL

更新日期：1982-08-01 00:00:00

abstract：:How calmodulin (CaM) acts in KRAS-driven cancers is a vastly important question. CaM binds to and stimulates PI3Kα/Akt signaling, promoting cell growth and proliferation. Phosphorylation of CaM at Tyr99 (pY99) enhances PI3Kα activation. PI3Kα is a lipid kinase. It phosphorylates PIP2 to produce PIP3, to which Akt bind...

journal_title：Biophysical journal

authors： Zhang M,Jang H,Gaponenko V,Nussinov R

更新日期：2017-11-07 00:00:00

abstract：:Nanopatterned surfaces are believed to kill bacteria through physical deformation, a mechanism that has immense potential against biochemical resistance. Because of its elusive nature, this mechanism is mostly understood through biophysical modeling. Problematically, accurate descriptions of the contact mechanics and ...

journal_title：Biophysical journal

authors： Velic A,Hasan J,Li Z,Yarlagadda PKDV

更新日期：2021-01-19 00:00:00

abstract：:Tendons are important load-bearing structures, which are frequently injured in both sports and work. Type I collagen fibrils are the primary components of tendons and carry most of the mechanical loads experienced by the tissue, however, knowledge of how load is transmitted between and within fibrils is limited. The p...

journal_title：Biophysical journal

authors： Svensson RB,Mulder H,Kovanen V,Magnusson SP

更新日期：2013-06-04 00:00:00

abstract：:Microbes inhabiting Earth have adapted to diverse environments of water, air, soil, and often at the interfaces of multiple media. In this study, we focus on the behavior of Caulobacter crescentus, a singly flagellated bacterium, at the air/water interface. Forward swimming C. crescentus swarmer cells tend to get phys...

journal_title：Biophysical journal

authors： Morse M,Huang A,Li G,Maxey MR,Tang JX

更新日期：2013-07-02 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

journal_title：Biophysical journal

authors： Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

更新日期：2016-08-09 00:00:00

abstract：:The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

journal_title：Biophysical journal

authors： Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

更新日期：2004-04-01 00:00:00

abstract：:Cholesterol is an essential molecule in the membranes of mammalian cells. It is known to be distributed heterogeneously within the cells, between the bilayer leaflets, as well as between lateral domains within the bilayer. However, we do not know exactly how cholesterol is distributed and what forces drive this sortin...

journal_title：Biophysical journal

authors： Nyholm TKM,Jaikishan S,Engberg O,Hautala V,Slotte JP

更新日期：2019-01-22 00:00:00