The nonequilibrium phase and glass transition behavior of beta-lactoglobulin.

abstract：:Coupled folding-binding is central to the function of many intrinsically disordered proteins, yet not fully understood. With a continuous three-letter protein model, we explore the free-energy landscape of pairs of interacting sequences and how it is impacted by 1), variations in the binding mechanism; and 2), the add...

journal_title：Biophysical journal

authors： Bhattacherjee A,Wallin S

更新日期：2012-02-08 00:00:00

abstract：:The interaction of Phormidium laminosum plastocyanin (PC) with P. laminosum cytochrome f (cyt f) was studied using Brownian dynamics (BD) simulations. Few complexes and a low rate of electron transfer were observed for wild-type PC. Increasing the positive electrostatic field on PC by the addition of a Zn(2+) ion in t...

journal_title：Biophysical journal

authors： Gross EL

更新日期：2004-09-01 00:00:00

abstract：:We report here several unusual features of inactivation of the rat Kv2.1 delayed rectifier potassium channel, expressed in Xenopus oocytes. The voltage dependence of inactivation was U-shaped, with maximum inactivation near 0 mV. During a maintained depolarization, development of inactivation was slow and only weakly ...

journal_title：Biophysical journal

authors： Klemic KG,Shieh CC,Kirsch GE,Jones SW

更新日期：1998-04-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:The neutral anesthetics chloroform and benzyl alcohol, at concentrations that block the nerve impulse, greatly modify the transport parameters of positive and negative ions in lipid bilayers made from monolayers. Both chloroform and benzyl alcohol increase the membrane permeability to these ions and increase the trans...

journal_title：Biophysical journal

authors： Reyes J,Latorre R

更新日期：1979-11-01 00:00:00

abstract：:The power spectrum of current fluctuations and the complex admittance of squid axon were determined in the frequency range 12.5 to 5,000 Hx during membrane voltage clamps to the same potentials in the same axon during internal perfusion with cesium. The complex admittance was determined rapidly and with high resolutio...

journal_title：Biophysical journal

authors： Fishman HM,Leuchtag HR,Moore LE

更新日期：1983-09-01 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These resu...

journal_title：Biophysical journal

authors： Sikorav JL,Pelta J,Livolant F

更新日期：1994-10-01 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and phospholamban (PLB) are essential for intracellular Ca2+ transport in myocytes. Ca2+-dependent activation of SERCA-PLB provides a control function that regulates cytosolic and SR Ca2+ levels. Although experimental and computational studies alone have led to a greater...

journal_title：Biophysical journal

authors： Raguimova ON,Aguayo-Ortiz R,Robia SL,Espinoza-Fonseca LM

更新日期：2020-11-03 00:00:00

abstract：:Many proteins are anchored to lipid bilayer membranes through a combination of hydrophobic and electrostatic interactions. In the case of the membrane-bound nonreceptor tyrosine kinase Src from Rous sarcoma virus, these interactions are mediated by an N-terminal myristoyl chain and an adjacent cluster of six basic ami...

journal_title：Biophysical journal

authors： Scheidt HA,Klingler J,Huster D,Keller S

更新日期：2015-08-04 00:00:00

abstract：:The statistical mechanics of cross-bridge action is considered in order to develop constitutive equations that express fiber tension as a function of degree of activation and time history of speed of contraction. The kinetic equation of A.F. Huxley (1) is generalized to apply to the partially activated state. The rate...

journal_title：Biophysical journal

authors： Tözeren A

更新日期：1985-02-01 00:00:00

abstract：:We have developed a novel, spectroscopic technique for high-sensitivity, label-free DNA quantification. We demonstrate that an optical resonance (whispering gallery mode) excited in a micron-sized silica sphere can be used to detect and measure nucleic acids. The surface of the silica sphere is chemically modified wit...

journal_title：Biophysical journal

authors： Vollmer F,Arnold S,Braun D,Teraoka I,Libchaber A

更新日期：2003-09-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backb...

journal_title：Biophysical journal

authors： Mak CH,Phan ENH

更新日期：2018-05-08 00:00:00

abstract：:A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we suggest that this motif maximizes information flow in a noisy network. Gene expressio...

journal_title：Biophysical journal

authors： Hormoz S

更新日期：2013-03-05 00:00:00

abstract：:The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

journal_title：Biophysical journal

authors： Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

更新日期：2015-09-15 00:00:00

abstract：:The hydrodynamic properties of rigid particles are calculated from models composed of spherical elements (beads) using theories developed by Kirkwood, Bloomfield, and their coworkers. Bead models have usually been built in such a way that the beads fill the volume occupied by the particles. Sometimes the beads are few...

journal_title：Biophysical journal

authors： Carrasco B,García de la Torre J

更新日期：1999-06-01 00:00:00

abstract：:The chromophore of octopus rhodopsin is 11-cis retinal, linked via a protonated Schiff base to the protein backbone. Its stable photoproduct, metarhodopsin, has all-trans retinal as its chromphore. The Schiff base of acid metarhodopsin (lambda max = 510 nm) is protonated, whereas that of alkaline metarhodopsin (lambda...

journal_title：Biophysical journal

authors： Koutalos Y,Ebrey TG,Gilson HR,Honig B

更新日期：1990-08-01 00:00:00

abstract：:Myo1b is a myosin that is exquisitely sensitive to tension. Its actin-attachment lifetime increases > 50-fold when its working stroke is opposed by 1 pN of force. The long attachment lifetime of myo1b under load raises the question: how are actin attachments that last >50 s in the presence of force regulated? Like mos...

journal_title：Biophysical journal

authors： Lewis JH,Greenberg MJ,Laakso JM,Shuman H,Ostap EM

更新日期：2012-06-20 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:Optical potentiometric indicators have been used to monitor the transmembrane electrical potential (Em) of many cells and organelles. A better understanding of the mechanisms of dye response is needed for the design of dyes with improved responses and for unambiguous interpretation of experimental results. This paper ...

journal_title：Biophysical journal

authors： Pratap PR,Novak TS,Freedman JC

更新日期：1990-04-01 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:We have examined the role of the environment on the interactions between transmembrane helices using, as a model system, the dimerization of the glycophorin A transmembrane helix. In this study we have focused on micellar environments and have examined a series of detergents that include a range of alkyl chain lengths...

journal_title：Biophysical journal

authors： Fisher LE,Engelman DM,Sturgis JN

更新日期：2003-11-01 00:00:00

abstract：:The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel swimming bacteria. Extensive experimental and theoretical studies exist on the structure, assembly, energy input, power generation, and switching mechanism of the motor. In a previous article, we explained the ge...

journal_title：Biophysical journal

authors： Bai F,Lo CJ,Berry RM,Xing J

更新日期：2009-04-22 00:00:00

abstract：:Verapamil and amlodipine are calcium ion influx inhibitors of wide clinical use. They are partially charged at neutral pH and exhibit amphiphilic properties. The noncharged species can easily cross the lipid membrane. We have measured with solid-state NMR the structural changes induced by verapamil upon incorporation ...

journal_title：Biophysical journal

authors： Meier M,Blatter XL,Seelig A,Seelig J

更新日期：2006-10-15 00:00:00

abstract：:The adhesion of giant unilamellar phospholipid vesicles to planar substrates coated with extracellular matrix mimetic cushions of hyaluronan is studied using quantitative reflection interference contrast microscopy. The absolute height of the vesicle membrane at the vicinity of the substrate is measured by considering...

journal_title：Biophysical journal

authors： Limozin L,Sengupta K

更新日期：2007-11-01 00:00:00

abstract：:The CLC family of chloride channels and transporters is a functionally diverse group of proteins important in a wide range of physiological processes. ClC-4 and ClC-5 are localized to endosomes and seem to play roles in the acidification of these compartments. These proteins were recently shown to function as Cl(-)/H(...

journal_title：Biophysical journal

authors： Osteen JD,Mindell JA

更新日期：2008-11-15 00:00:00

abstract：:The mathematical model of steady-state iron kinetics in the human presented here extends the use of radioactive tracer observations in the determination of erythron maturative and proliferative behavior. Functions descriptive of that behavior appear explicitly in the model together with functions customarily measured ...

journal_title：Biophysical journal

authors： Nooney GC

更新日期：1965-11-01 00:00:00

abstract：:The vibrational energy relaxation of a selected vibrational mode in cytochrome c--a C-D stretch in the terminal methyl group of Met80--has been studied using equilibrium molecular dynamics simulation and normal mode analysis methods. As demonstrated in the pioneering work of Romesberg and co-workers, isotopic labeling...

journal_title：Biophysical journal

authors： Bu L,Straub JE

更新日期：2003-09-01 00:00:00