Inactivation of Kv2.1 potassium channels.

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was develope...

journal_title：Biophysical journal

authors： Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

更新日期：2006-12-15 00:00:00

abstract：:An algorithm needed for computer simulation of immunodiffusion has been deduced from existing theories of the in vitro reaction between antibody and antigen. The "Goldberg most probable polymer distribution" theory provides a formula that gives the amount of free antibody, free antigen, and diffusible complexes from e...

journal_title：Biophysical journal

authors： Trautman R

更新日期：1973-05-01 00:00:00

abstract：:A discussion is given of the general role of diffusion in enzyme kinetics based upon a rigorous theory for bimolecular association and dissociation steps that was presented previously, and criteria are formulated for the dependence of the over-all rate on medium viscosity. With these criteria it is possible to conclud...

journal_title：Biophysical journal

authors： Schurr JM

更新日期：1970-08-01 00:00:00

abstract：:The lateral mobility of the lipid analog N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine and of the integral protein glycophorin in giant dimyristoylphosphatidylcholine vesicles was studied by the photobleaching technique. Above the temperature of the chain-melting transition (Tm = 23 degrees C), the diffusi...

journal_title：Biophysical journal

authors： Kapitza HG,Rüppel DA,Galla HJ,Sackmann E

更新日期：1984-03-01 00:00:00

abstract：:Using a combination of microelectrode measurements and high-power microscopy we have demonstrated that different Donnan potentials can be recorded from the A- and I-bands of glycerinated and chemically skinned muscles in rigor, so that the A-band fixed charge concentration exceeds the I-band fixed charge concentration...

journal_title：Biophysical journal

authors： Bartels EM,Elliott GF

更新日期：1985-07-01 00:00:00

abstract：:This paper describes the relation between the quantum yield of photosynthesis in the red alga Porphyridium cruentum, and the spectral composition of light, changed by filtering white light through aqueous phycobilin solutions of increasing optical density. At sufficiently high densities of the filter solution, no meas...

journal_title：Biophysical journal

authors： THOMAS JB,GOVINDJEE

更新日期：1960-09-01 00:00:00

abstract：:In the photoactive yellow protein, PYP, both Glu46 and Tyr42 form hydrogen bonds to the phenolic OH group of the p-hydroxycinnamoyl chromophore. Previous work on replacement of the carboxyl group of Glu46 by an amide group (Glu46Gln) has shown that changing the nature of this hydrogen bond has a minimal effect on the ...

journal_title：Biophysical journal

authors： Devanathan S,Lin S,Cusanovich MA,Woodbury N,Tollin G

更新日期：2001-10-01 00:00:00

abstract：:Myosin subfragment 1 (S1) can be specifically modified at Lys-553 with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) (Bertrand, R., J. Derancourt, and R. Kassab. 1995. Biochemistry. 34:9500-9507), and solvent quenching of FHS-S1 with iodide has been shown to be sensit...

journal_title：Biophysical journal

authors： Cooper WC,Chrin LR,Berger CL

更新日期：2000-03-01 00:00:00

abstract：:Gap junctions formed of connexin46 (Cx46) and connexin50 (Cx50) in lens fiber cells are crucial for maintaining lens transparency. We determined the functional properties of homotypic Cx46, heterotypic Cx46/Cx50, and heteromeric Cx46/Cx50 channels in a communication-deficient neuroblastoma (N2A) cell line, using dual ...

journal_title：Biophysical journal

authors： Hopperstad MG,Srinivas M,Spray DC

更新日期：2000-10-01 00:00:00

abstract：:Normal coordinate analyses and vibrational assignments are presented for the dimethyl phosphate anion [(CH3O)2PO2-] and its deuteriomethyl [(CD3O)2PO2-] and carbon-13 [(13CH3O)2PO2-] derivatives in the gauche-gauche conformation. The dimethyl phosphate anion, which is the simplest model for the nucleic acid phosphodie...

journal_title：Biophysical journal

authors： Guan Y,Wurrey CJ,Thomas GJ Jr

更新日期：1994-01-01 00:00:00

abstract：:We report that long-chain poly-L-glutamine forms cation-selective channels when incorporated into artificial planar lipid bilayer membranes. The channel was permeable to alkali cations and H(+) ions and virtually impermeable to anions; the selectivity sequence based on the single-channel conductance was H(+) > Cs(+) >...

journal_title：Biophysical journal

authors： Monoi H,Futaki S,Kugimiya S,Minakata H,Yoshihara K

更新日期：2000-06-01 00:00:00

abstract：:Object contrast is one of the most important parameters of macromolecular imaging. Low-voltage transmission electron microscopy has shown an increased atom contrast for carbon materials, indicating that amplitude contrast contributions increase at a higher rate than phase contrast and inelastic scattering. Here, we st...

journal_title：Biophysical journal

authors： Majorovits E,Angert I,Kaiser U,Schröder RR

更新日期：2016-02-23 00:00:00

abstract：:There is no methodology for the estimation of the dynamic features of large-molecular-weight RNAs in homogeneous physiological media. In this report, a luminescence anisotropy-based method using a long-lifetime luminescent oligonucleotide probe for the estimation of the dynamic features of large-molecular-weight RNA i...

journal_title：Biophysical journal

authors： Sakamoto T,Mahara A,Yamagata K,Iwase R,Yamaoka T,Murakami A

更新日期：2005-12-01 00:00:00

abstract：:Biological cells embedded in fibrous matrices have been observed to form intercellular bands of dense and aligned fibers through which they mechanically interact over long distances. Such matrix-mediated cellular interactions have been shown to regulate various biological processes. This study aimed to explore the eff...

journal_title：Biophysical journal

authors： Sopher RS,Tokash H,Natan S,Sharabi M,Shelah O,Tchaicheeyan O,Lesman A

更新日期：2018-10-02 00:00:00

abstract：:Flagellin is the subunit of the bacterial filament, the micrometer-long propeller of a bacterial flagellum. The protein is believed to undergo unfolding for transport through the channel of the filament and to refold in a chamber at the end of the channel before being assembled into the growing filament. We report a t...

journal_title：Biophysical journal

authors： Chng CP,Kitao A

更新日期：2008-05-15 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:Collagen fibrils form extracellular networks that regulate cell functions and provide mechanical strength to tissues. Collagen fibrillogenesis is an entropy-driven process promoted by warming and reversed by cooling. Here, we investigate the influence of noncovalent interactions mediated by the collagen triple helix o...

journal_title：Biophysical journal

authors： de Wild M,Pomp W,Koenderink GH

更新日期：2013-07-02 00:00:00

abstract：:The enzyme F1-adenosine triphosphatase (ATPase) is a molecular motor that converts the chemical energy stored in the molecule adenosine triphosphate (ATP) into mechanical rotation of its gamma-subunit. During steady-state catalysis, the three catalytic sites of F1 operate in a cooperative fashion such that at every in...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2004-11-01 00:00:00

abstract：:High-bandwidth measurements of the ion current through hafnium oxide and silicon nitride nanopores allow the analysis of sub-30 kD protein molecules with unprecedented time resolution and detection efficiency. Measured capture rates suggest that at moderate transmembrane bias values, a substantial fraction of protein ...

journal_title：Biophysical journal

authors： Larkin J,Henley RY,Muthukumar M,Rosenstein JK,Wanunu M

更新日期：2014-02-04 00:00:00

abstract：:Huntington's disease is a heritable neurodegenerative disease that is caused by a CAG expansion in the first exon of the huntingtin gene. This expansion results in an elongated polyglutamine domain that increases the propensity of huntingtin exon-1 to form cross-β fibrils. Although the polyglutamine domain is importan...

journal_title：Biophysical journal

authors： Falk AS,Bravo-Arredondo JM,Varkey J,Pacheco S,Langen R,Siemer AB

更新日期：2020-11-17 00:00:00

abstract：:The thylakoid membrane has a unique lipid composition, consisting mostly of galactolipids. These thylakoid lipids have important roles in photosynthesis. Here, we investigate to what extent these lipids bind specifically to the Photosystem II complex. To this end, we performed coarse-grain MD simulations of the Photos...

journal_title：Biophysical journal

authors： Van Eerden FJ,Melo MN,Frederix PWJM,Marrink SJ

更新日期：2017-12-19 00:00:00

abstract：:Voltage-gated ion channels are crucial for regulation of electric activity of excitable tissues such as nerve cells, and play important roles in many diseases. During activation, the charged S4 segment in the voltage sensor domain translates across a hydrophobic core forming a barrier for the gating charges. This barr...

journal_title：Biophysical journal

authors： Schwaiger CS,Liin SI,Elinder F,Lindahl E

更新日期：2013-01-08 00:00:00

abstract：:The mechanisms by which extracellular electric field stimuli induce the (re)excitation of cardiac cells in various stages of refractoriness are still not well understood. We modeled the interactions between an isolated cardiac cell and imposed extracellular electric fields to determine the mechanisms by which relative...

journal_title：Biophysical journal

authors： Fishler MG,Sobie EA,Thakor NV,Tung L

更新日期：1996-03-01 00:00:00

abstract：:The absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. To meet this challenge we present an approach to construct a convex space, termed the k-cone, which c...

journal_title：Biophysical journal

authors： Famili I,Mahadevan R,Palsson BO

更新日期：2005-03-01 00:00:00

abstract：:Universal features of the peptide aggregation process suggest a common mechanism, with a first-order phase transition in aqueous solutions of the peptides being the driving force. Small system sizes strongly affect the stability of the minor phase in the two-phase region. We show manifestations of this effect in aqueo...

journal_title：Biophysical journal

authors： Singh G,Brovchenko I,Oleinikova A,Winter R

更新日期：2008-10-01 00:00:00

abstract：:Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...

journal_title：Biophysical journal

authors： Oelz DB,Del Castillo U,Gelfand VI,Mogilner A

更新日期：2018-10-16 00:00:00

abstract：:We elucidate the physics of protein dynamical transition via 10-100-ns molecular dynamics simulations at temperatures spanning 160-300 K. By tracking the energy fluctuations, we show that the protein dynamical transition is marked by a crossover from nonstationary to stationary processes that underlie the dynamics of ...

journal_title：Biophysical journal

authors： Okan OB,Atilgan AR,Atilgan C

更新日期：2009-10-07 00:00:00

abstract：:Rearrangement of tertiary structure in response to mechanical force (termed tertiary structure elasticity) in the tandem Ig chain is the first mode of elastic response for muscle protein titin. Tertiary structure elasticity occurs at low stretching forces (few tens of pN), and was described at atomic resolution in a r...

journal_title：Biophysical journal

authors： Hsin J,Schulten K

更新日期：2011-02-16 00:00:00

abstract：:The difference in the surface charge distribution between light-adapted and dark-adapted purple membranes was investigated with electric dichroism measurements from approximately pH 5 to pH 11. Purple membrane sheets in solution are oriented in a weak electric field by their permanent dipole moment, which is due to th...

journal_title：Biophysical journal

authors： Otomo J,Ohno K,Takeuchi Y,Ikegami A

更新日期：1986-08-01 00:00:00