Abstract:
:This paper describes the relation between the quantum yield of photosynthesis in the red alga Porphyridium cruentum, and the spectral composition of light, changed by filtering white light through aqueous phycobilin solutions of increasing optical density. At sufficiently high densities of the filter solution, no measurable photosynthesis can be observed, although chlorophyll a molecules are still being excited at a significant rate, as can be proved by calculations from spectral distribution curves, and is confirmed by the occurrence of a "second Emerson effect" upon addition of orange light. An interpretation of this result, based on other experiments, will be given in a subsequent paper. A modification of the opal glass technique for reducing the effect of scattering when measuring absorption, was developed in connection with this research, and also is described in the paper.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
THOMAS JB,GOVINDJEEdoi
10.1016/s0006-3495(60)86876-2subject
Has Abstractpub_date
1960-09-01 00:00:00pages
63-72eissn
0006-3495issn
1542-0086pii
S0006-3495(60)86876-2journal_volume
1pub_type
杂志文章abstract::The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.057059
更新日期:2005-04-01 00:00:00
abstract::Small, icosahedral double-stranded DNA bacteriophage pack their genomes tightly into preformed protein capsids using an ATP-driven motor. Coarse-grain molecular-mechanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformation depends on capsid size and shape, and the presence o...
journal_title:Biophysical journal
pub_type: 杂志文章,评审
doi:10.1529/biophysj.108.131797
更新日期:2008-07-01 00:00:00
abstract::Cellular processes are noisy due to the stochastic nature of biochemical reactions. As such, it is impossible to predict the exact quantity of a molecule or other attributes at the single-cell level. However, the distribution of a molecule over a population is often deterministic and is governed by the underlying regu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2014.07.025
更新日期:2014-09-02 00:00:00
abstract::Two mechanisms have been proposed to account for solute permeation of lipid bilayers. Partitioning into the hydrophobic phase of the bilayer, followed by diffusion, is accepted by many for the permeation of water and other small neutral solutes, but transient pores have also been proposed to account for both water and...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79575-9
更新日期:1996-01-01 00:00:00
abstract::Various differential polarization images or Mueller images of model objects are generated using the equations derived in the previous paper (paper I of this series). These calculated images include models of the higher-order organization of metaphase chromosomes, and show the applicability of the differential polariza...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(87)83286-1
更新日期:1987-12-01 00:00:00
abstract::We have constructed a model of the upper part of the glycolysis in the pancreatic beta-cell. The model comprises the enzymatic reactions from glucokinase to glyceraldehyde-3-phosphate dehydrogenase (GAPD). Our results show, for a substantial part of the parameter space, an oscillatory behavior of the glycolysis for a ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74460-9
更新日期:2003-07-01 00:00:00
abstract::The problem of the desorption of a lipid molecule from a lipid vesicle (donor) and its incorporation into another vesicle (acceptor) at high acceptor concentrations, which has been investigated experimentally (Jones, J. D. and Thompson, T. E., 1990. Biochemistry, 29:1593-1600), is analyzed here from a theoretical poin...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(99)77351-0
更新日期:1999-04-01 00:00:00
abstract::The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79767-9
更新日期:1996-04-01 00:00:00
abstract::The accurate prediction of membrane-insertion probability for arbitrary protein sequences is a critical challenge to identifying membrane proteins and determining their folded structures. Although algorithms based on sequence statistics have had moderate success, a complete understanding of the energetic factors that ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2012.01.021
更新日期:2012-02-22 00:00:00
abstract::Chlamydomonas exhibits a sequence of a photoreceptor current and two flagellar currents upon stimulation with bright green flashes. The currents are thought to be a prerequisite for the well-known photophobic responses. In the preceding paper, we analyzed the kinetics of these currents and their dependence on extracel...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79636-4
更新日期:1996-02-01 00:00:00
abstract::Downstream processes that discriminate between DNA adducts of a third generation platinum antitumor drug oxaliplatin and conventional cisplatin are believed to be responsible for the differences in their biological effects. These different biological effects are explained by the ability of oxaliplatin to form DNA addu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.116996
更新日期:2007-12-01 00:00:00
abstract::The diffusion of Ca as it converges to the external mouth of a Ca channel is examined. Diffusional limitation on Ca ions entering Ca channels during current flow, cause local extracellular Ca depletions. Such extracellular Ca depletions have been reported in cardiac muscle. The cardiac sarcolemma has a large number of...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(91)82284-6
更新日期:1991-03-01 00:00:00
abstract::Time courses of myelin lattice swelling in toad sciatic nerves preexposed to different treatments were determined by x-ray diffraction using a one-dimensional position-sensitive detector. In the nerves supramaximally stimulated for 1 h at 200 Hz, the subsequent process of myelin swelling occurred 45.0 +/- 7.3 min (n =...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(82)84506-2
更新日期:1982-08-01 00:00:00
abstract::To investigate the mechanisms by which vesicles of pulmonary surfactant adsorb to an air-liquid interface, we measured the effect of different phospholipids and of their concentration both in the subphase and at the interface on this process. Adsorbing vesicles contained the hydrophobic surfactant proteins mixed with ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(00)76589-1
更新日期:2000-01-01 00:00:00
abstract::In recent years, optical sensors for tracking neural activity have been developed and offer great utility. However, developing microscopy techniques that have several kHz bandwidth necessary to reliably capture optically reported action potentials (APs) at multiple locations in parallel remains a significant challenge...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2014.12.005
更新日期:2015-02-03 00:00:00
abstract::The analysis of single-molecule fluorescence resonance energy transfer (FRET) trajectories has become one of significant biophysical interest. In deducing the transition rates between various states of a system for time-binned data, researchers have relied on simple, but often arbitrary methods of extracting rates fro...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.082487
更新日期:2006-09-01 00:00:00
abstract::Photosystem II catalyzes photosynthetic water oxidation in plants, green algae, and cyanobacteria. The manganese-containing active site cycles through a series of five oxidation states, S(n), where n refers to the number of oxidizing equivalents stored. In this report, reaction-induced Fourier transform infrared and e...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74680-3
更新日期:2003-10-01 00:00:00
abstract::Applying self-consistent field theory, we consider a coarse-grained model for the polymerlike projections of neurofilament (NF) proteins that form a brush structure around neurofilaments. We focus on effects of molecular composition, which is the relative occurrence of NF-H, NF-M, and NF-L proteins, on the organizatio...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.10.033
更新日期:2010-02-03 00:00:00
abstract::Elongation of the mitotic spindle during anaphase B contributes to chromosome segregation in many cells. Here, we quantitatively test the ability of two models for spindle length control to describe the dynamics of anaphase B spindle elongation using experimental data from Drosophila embryos. In the slide-and-flux-or-...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2015.03.018
更新日期:2015-04-21 00:00:00
abstract::Cholesterol is essential to the formation of phase-separated lipid domains in membranes. Lipid domains can exist in different thermodynamic phases depending on the molecular composition and play significant roles in determining structure and function of membrane proteins. We investigate the role of cholesterol in the ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2018.10.011
更新日期:2018-12-04 00:00:00
abstract::We extend the Saffman theory of membrane hydrodynamics to account for the correlated motion of membrane proteins, along with the effect of protein concentration on that correlation and on the response of the membrane to stresses. Expressions for the coupling diffusion coefficients of protein pairs and their concentrat...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.01.020
更新日期:2009-04-22 00:00:00
abstract::A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(83)84278-7
更新日期:1983-10-01 00:00:00
abstract::Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79444-4
更新日期:1996-11-01 00:00:00
abstract::Optical mapping with voltage-sensitive dyes provides a high-resolution technique to observe cardiac electrodynamic behavior. Although most studies assume that the fluorescent signal is emitted from the surface layer of cells, the effects of signal attenuation with depth on signal interpretation are still unclear. This...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74825-5
更新日期:2003-12-01 00:00:00
abstract::Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2014.02.040
更新日期:2014-05-20 00:00:00
abstract::The well-characterized integral membrane protein lactose (lac) permease from Escherichia coli was reconstituted together with trace amounts (molar fraction X = 0.005 of the total phospholipid) of different pyrene-labeled phospholipid analogs into 1-palmitoyl-2-oleoyl-sn-glycero-3-sn-glycero-3-phospho-rac'-glycerol (PO...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(97)78771-X
更新日期:1997-03-01 00:00:00
abstract::Xenopus melanophores have pigment organelles or melanosomes which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. Melanosomes are transported by microtubule motors, kinesin-2 and cytoplasmic dynein, and an actin motor, myosin-V. We explored the regulation of melanosome trans...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.067843
更新日期:2006-01-01 00:00:00
abstract::The electronic structure of the circular aggregate of 18 bacteriochlorophyll a (BChl a) molecules responsible for the B850 absorption band of the light-harvesting 2 (LH2) complex of the photosynthetic purple bacterium Rhodopseudomonas acidophila has been studied by measuring fluorescence-excitation spectra of individu...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)76132-2
更新日期:2001-03-01 00:00:00
abstract::Diphtheria toxin (DT) contains separate domains for receptor-specific binding, translocation, and enzymatic activity. After binding to cells, DT is taken up into endosome-like acidic compartments where the translocation domain inserts into the endosomal membrane and releases the catalytic domain into the cytosol. The ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.113498
更新日期:2008-03-15 00:00:00
abstract::The skeletal muscles of chickens, frogs, and fish have been reported to express two isoforms (alpha and beta) of the sarcoplasmic reticulum calcium release channel (ryanodine receptor or RYR), while mammals express only one. We have studied patterns of RYR isoform expression in skeletal muscles from a variety of fish,...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(93)81303-1
更新日期:1993-12-01 00:00:00