Imaging the traction stresses exerted by locomoting cells with the elastic substratum method.

abstract：:We examined the kinetics of voltage-dependent sodium currents in cerebellar Purkinje neurons using whole-cell recording from dissociated neurons. Unlike sodium currents in other cells, recovery from inactivation in Purkinje neurons is accompanied by a sizeable ionic current. Additionally, the extent and speed of recov...

journal_title：Biophysical journal

authors： Raman IM,Bean BP

更新日期：2001-02-01 00:00:00

abstract：:This paper is concerned with the nature of the transmembrane potential induced in an inactive nerve fiber lying in an impressed potential field within a nerve trunk. The impressed potential field is assumed to be produced by the synchronous activity of other fibers within the trunk; an expression for the induced trans...

journal_title：Biophysical journal

authors： Clark JW Jr,Plonsey R

更新日期：1971-03-01 00:00:00

abstract：:The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time...

journal_title：Biophysical journal

authors： Korgel BA,van Zanten JH,Monbouquette HG

更新日期：1998-06-01 00:00:00

abstract：:Most of the cellular ATP in living organisms is synthesized by the enzyme F(1)F(o)-ATP synthase. The water soluble F(1) part of the enzyme can also work in reverse and utilize the chemical energy released during ATP hydrolysis to generate mechanical motion. Despite the availability of a large amount of biochemical dat...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2003-10-01 00:00:00

abstract：:In wild-type lenses from various species, an intracellular hydrostatic pressure gradient goes from ∼340 mmHg in central fiber cells to 0 mmHg in surface cells. This gradient drives a center-to-surface flow of intracellular fluid. In lenses in which gap-junction coupling is increased, the central pressure is lower, whe...

journal_title：Biophysical journal

authors： Gao J,Sun X,White TW,Delamere NA,Mathias RT

更新日期：2015-11-03 00:00:00

abstract：:The molecular motor protein myosin VI moves toward the minus-end of actin filaments with a step size of 30-36 nm. Such large step size either drastically limits the degree of complex formation between dimer subunits to leave enough length for the lever arms, or requires an extension of the lever arms' crystallographic...

journal_title：Biophysical journal

authors： Liu Y,Hsin J,Kim H,Selvin PR,Schulten K

更新日期：2011-06-22 00:00:00

abstract：:To study why pancreatic beta-cells prefer to burst as a multi-cellular complex, we have formulated a stochastic model for bursting clusters of excitable cells. Our model incorporated a delayed rectifier K+ channel, a fast voltage-gated Ca2+ channel, and a slow Cai-blockable Ca2+ channel. The fraction of ATP-sensitive ...

journal_title：Biophysical journal

authors： Chay TR,Kang HS

更新日期：1988-09-01 00:00:00

abstract：:We have used electron paramagnetic resonance (EPR) to probe the homo- and heterooligomeric interactions of reconstituted sarcoplasmic reticulum Ca-ATPase (SERCA) and its regulator phospholamban (PLB). SERCA is responsible for restoring calcium to the sarcoplasmic reticulum to allow muscle relaxation, whereas PLB inhib...

journal_title：Biophysical journal

authors： James ZM,McCaffrey JE,Torgersen KD,Karim CB,Thomas DD

更新日期：2012-09-19 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:Observation of cell membrane buckling and cell folding in micropipette aspiration experiments was used to evaluate the bending rigidity of the red blood cell membrane. The suction pressure required to buckle the membrane surface initially was found to be about one-half to two-thirds of the pressure that caused the cel...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1983-07-01 00:00:00

abstract：:Surface and subsurface dynamics of Rat Basophilic Leukemia cells, a model system of stimulated secretion, were imaged using Scanning Force Microscopy (SFM) at a rate of 50-60 s/image. Cytoskeletal elements and organelles were tracked within quiescent cells and those activated after IgE receptor crosslinking. In additi...

journal_title：Biophysical journal

authors： Braunstein D,Spudich A

更新日期：1994-05-01 00:00:00

abstract：:The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, w...

journal_title：Biophysical journal

authors： Kukita F,Yamagishi S

更新日期：1981-07-01 00:00:00

abstract：:Voltage-sensitive fluorescent dyes are commonly used to measure cardiac electrical activity. Recent studies indicate, however, that optical action potentials (OAPs) recorded from the myocardial surface originate from a widely distributed volume beneath the surface and may contain useful information regarding intramura...

journal_title：Biophysical journal

authors： Hyatt CJ,Mironov SF,Wellner M,Berenfeld O,Popp AK,Weitz DA,Jalife J,Pertsov AM

更新日期：2003-10-01 00:00:00

abstract：:Despite the spontaneity of some in vitro protein-folding reactions, native folding in vivo often requires the participation of barrel-shaped multimeric complexes known as chaperonins. Although it has long been known that chaperonin substrates fold upon sequestration inside the chaperonin barrel, the precise mechanism ...

journal_title：Biophysical journal

authors： England JL,Pande VS

更新日期：2008-10-01 00:00:00

abstract：:The potassium selective, inward rectifier current (IK1) is known to be responsible for maintaining the resting membrane potential of quiescent ventricular myocytes. However, the contribution of this current to the different phases of the cardiac action potential has not been adequately established. In the present stud...

journal_title：Biophysical journal

authors： Ibarra J,Morley GE,Delmar M

更新日期：1991-12-01 00:00:00

abstract：:Formamide-induced detubulation of rat ventricular myocytes was used to investigate the functional distribution of the Na/Ca exchanger (NCX) and Na/K-ATPase between the t-tubules and external sarcolemma. Detubulation resulted in a 32% decrease in cell capacitance, whereas cell volume was unchanged. Thus, the surface-to...

journal_title：Biophysical journal

authors： Despa S,Brette F,Orchard CH,Bers DM

更新日期：2003-11-01 00:00:00

abstract：:The engulfment function of macrophages relies on complex molecular interactions involving both lipids and proteins. In particular, the clearance of apoptotic bodies (efferocytosis) is enabled by externalization on the cell target of phosphatidylserine lipids, which activate receptors on macrophages, suggesting that (l...

journal_title：Biophysical journal

authors： Justice MJ,Petrusca DN,Rogozea AL,Williams JA,Schweitzer KS,Petrache I,Wassall SR,Petrache HI

更新日期：2014-02-04 00:00:00

abstract：:An analytical electromechanical model of a spherical cell exposed to an alternating electric field was used to calculate shear stress generated in the cellular membrane. Shape deformation of Neurospora crassa (slime) spheroplasts was measured. Statistical analysis permitted empirical evaluation of creep of the cellula...

journal_title：Biophysical journal

authors： Poznański J,Pawłowski P,Fikus M

更新日期：1992-03-01 00:00:00

abstract：:Root mean-square deviation (RMSD) after roto-translational least-squares fitting is a measure of global structural similarity of macromolecules used commonly. On the other hand, experimental x-ray B-factors are used frequently to study local structural heterogeneity and dynamics in macromolecules by providing direct i...

journal_title：Biophysical journal

authors： Kuzmanic A,Zagrovic B

更新日期：2010-03-03 00:00:00

abstract：:Hyaluronan (HA) is a highly hydrated polyanion, which is a network-forming and space-filling component in the extracellular matrix of animal tissues. Confocal fluorescence recovery after photobleaching (confocal-FRAP) was used to investigate intramolecular hydrogen bonding and electrostatic interactions in hyaluronan ...

journal_title：Biophysical journal

authors： Gribbon P,Heng BC,Hardingham TE

更新日期：1999-10-01 00:00:00

abstract：:Hundreds of cationic antimicrobial and cell-penetrating peptides (CPPs) form amphipathic α-helices when bound to lipid membranes. Here, we test two hypotheses for the differences in the ability of these peptides to translocate across membranes. The first, which we now call the hydrophobicity hypothesis, is that peptid...

journal_title：Biophysical journal

authors： Ablan FDO,Spaller BL,Abdo KI,Almeida PF

更新日期：2016-10-18 00:00:00

abstract：:Proliferation of mammalian cells, even under conditions of unlimited growth, presents a complex problem because of the interaction of deterministic and stochastic processes. Division of the cell cycle into a finite number of parts establishes a multidimensional vector space. In this space an arbitrary culture can be r...

journal_title：Biophysical journal

authors： Hahn GM

更新日期：1966-05-01 00:00:00

abstract：:A number of properties of certain living embryonic tissues can be explained by considering them as liquids. Tissue fragments left in a shaker bath round up to form spherical aggregates, as do liquid drops. When cells comprising two distinct embryonic tissues are mixed, typically a nucleation-like process takes place, ...

journal_title：Biophysical journal

authors： Forgacs G,Foty RA,Shafrir Y,Steinberg MS

更新日期：1998-05-01 00:00:00

abstract：:We report on the application of two photon molecular excitation to fluorescence correlation spectroscopy. We demonstrate the first fluorescence correlation spectroscopy measurements of translational mobility in the cytoplasm of living cells. Two-photon excitation inherently excites small sample volumes in three dimens...

journal_title：Biophysical journal

authors： Berland KM,So PT,Gratton E

更新日期：1995-02-01 00:00:00

abstract：:Biological systems often have to measure extremely low concentrations of chemicals with high precision. When dealing with such small numbers of molecules, the inevitable randomness of physical transport processes and binding reactions will limit the precision with which measurements can be made. An important question ...

journal_title：Biophysical journal

authors： Kaizu K,de Ronde W,Paijmans J,Takahashi K,Tostevin F,ten Wolde PR

更新日期：2014-02-18 00:00:00

abstract：:A kinetic model is proposed to reproduce the time courses of the concentration change in paused leader RNA, terminated leader RNA, and readthrough RNA in the single-round transcription experiments on trp leader region of Escherichia coli and its mutants, L132, L75, and L75L135 (Winkler, M. E., and C. Yanofsky, 1981, B...

journal_title：Biophysical journal

authors： Suzuki H,Kunisawa T,Otsuka J

更新日期：1987-03-01 00:00:00

abstract：:Small RNA-mRNA binding is an essential step in RNA interference, an important cellular regulatory process. Calculations of binding free energy have been used in binding site prediction, but the cost of stretching the mRNA loop when the small RNA-mRNA duplex forms requires further exploration. Here, using both polymer ...

journal_title：Biophysical journal

authors： Meng Y,Aalberts DP

更新日期：2013-01-22 00:00:00

abstract：:We have investigated the phase behavior of self-assembled lyotropic liquid crystals (LC) formed by ternary mixtures of oleoylethanolamide (OEA), water and arginine. OEA, a natural analog of the endogenous cannabinoid anandamide involved in the peripheral regulation of feeding, was selected as a main component due to i...

journal_title：Biophysical journal

authors： Mohammady SZ,Pouzot M,Mezzenga R

更新日期：2009-02-18 00:00:00

abstract：:Binding features of heroin in whole blood and in blood fractions were delineated by measuring the selective spin-lattice relaxation rates of heroin protons in physiologic conditions. Interaction with some receptor located in the whole human blood or in the human plasma was detected and the apparent binding constant ca...

journal_title：Biophysical journal

authors： Barni Comparini I,Gaggelli E,Marchettini N,Valensin G

更新日期：1985-08-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00