Abstract:
:Small, icosahedral double-stranded DNA bacteriophage pack their genomes tightly into preformed protein capsids using an ATP-driven motor. Coarse-grain molecular-mechanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformation depends on capsid size and shape, and the presence or absence of a protein core. The forces that oppose packaging have large contributions from both electrostatic repulsions and the entropic penalty of confining the DNA into the capsid, whereas elastic deformations make only a modest contribution. The elastic deformation energy is very sensitive to the final conformation, whereas the electrostatic and entropic penalties are not, so the packaged DNA favors conformations that minimize the bending energy.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Petrov AS,Harvey SCdoi
10.1529/biophysj.108.131797subject
Has Abstractpub_date
2008-07-01 00:00:00pages
497-502issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(08)70226-1journal_volume
95pub_type
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