Model-driven designs of an oscillating gene network.

abstract：:The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

journal_title：Biophysical journal

authors： Berdiev BK,Latorre R,Benos DJ,Ismailov II

更新日期：2001-05-01 00:00:00

abstract：:ATP, 2-deoxy ATP (dATP), CTP, and UTP support isometric force and unloaded shortening velocity (Vu) to various extents (Regnier et al., Biophys. J. 74:3044-3058). Vu correlated with the rate of cross-bridge dissociation after the power stroke and the steady-state hydrolysis rate in solution, whereas force was modulate...

journal_title：Biophysical journal

authors： Regnier M,Homsher E

更新日期：1998-06-01 00:00:00

abstract：:Conduction speed (theta) in single myelinated Rana pipiens sciatic nerve fibers has been precisely measured using intracellular recording and on-line digital computer techniques. The dependence of relative speed on external Na concentration at 15 degrees C has been found to be ln(theta(1)/theta(2)) = 0.524 (+/-0.018) ...

journal_title：Biophysical journal

authors： Hardy WL

更新日期：1973-10-01 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:The effect of ionic strength on the macroscopic and microscopic redox potentials and the heme environment of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F have been investigated by NMR and electrochemical methods. The redox potentials of this tetraheme protein are found to be ionic strength-dependent. Especiall...

journal_title：Biophysical journal

authors： Ohmura T,Nakamura H,Niki K,Cusanovich MA,Akutsu H

更新日期：1998-09-01 00:00:00

abstract：:Recent work has introduced a new fluorescent voltage sensor, ASAP1, which can monitor rapid trains of action potentials in cultured neurons. This indicator is based on the Gallus gallus voltage-sensitive phosphatase with the phosphatase domain removed and a circularly permuted GFP placed in the S3-S4 linker. However, ...

journal_title：Biophysical journal

authors： Lee EEL,Bezanilla F

更新日期：2017-11-21 00:00:00

abstract：:Chemotactic effects of dissolved oxygen on motions of Escherichia coli in a motility buffer solution have been studied by measurements of quasielastic light scattering. Under conditions where the bacteria form a sharp band in an oxygen concentrations gradient created by their metabolism, components of motions along th...

journal_title：Biophysical journal

authors： Wang PC,Chen SH

更新日期：1981-10-01 00:00:00

abstract：:Influenza neuraminidase is an important drug target. Glycans are present on neuraminidase and are generally considered to inhibit antibody binding via their glycan shield. In this work, we studied the effect of glycans on the binding kinetics of antiviral drugs to the influenza neuraminidase. We created all-atom in si...

journal_title：Biophysical journal

authors： Seitz C,Casalino L,Konecny R,Huber G,Amaro RE,McCammon JA

更新日期：2020-12-01 00:00:00

abstract：:A dissipative system is approximated by a nonlinear rate equation: Z congruent to K1Z - K2Z3 (K2 greater than 0), in which the right side is derived from -delta G/delta Z of Taylor's series of the thermodynamic potential given by Gibbs' function G(Tc, Pc) (Z) at about the critical point C(Tc, Pc) of the control variab...

journal_title：Biophysical journal

authors： Shirane K,Tokimoto T,Yamaguchi Y

更新日期：1990-01-01 00:00:00

abstract：:A new method for the investigation of ion translocating membrane proteins is presented. Protein containing membrane fragments or vesicles are adsorbed to a solid supported membrane. The solid supported membrane consists of a lipid monolayer on a gold evaporated or gold sputtered glass substrate which is coated with a ...

journal_title：Biophysical journal

authors： Seifert K,Fendler K,Bamberg E

更新日期：1993-02-01 00:00:00

abstract：:Several suggestions have been made about the functional significance of dendritic spines in connection with synaptic plasticity. We investigated transient electrical behavior of spines with bulbous terminals in neurons with arbitrary dendritic geometries. It is shown that postsynaptic potential transform caused by a s...

journal_title：Biophysical journal

authors： Kawato M,Tsukahara N

更新日期：1984-08-01 00:00:00

abstract：:There are several examples of membrane-associated protein domains that target curved membranes. This behavior is believed to have functional significance in a number of essential pathways, such as clathrin-mediated endocytosis, which involve dramatic membrane remodeling and require the recruitment of various cofactors...

journal_title：Biophysical journal

authors： Cui H,Lyman E,Voth GA

更新日期：2011-03-02 00:00:00

abstract：:The dynamical behavior of biomacromolecules is a fundamental property regulating a large number of biological processes. Protein dynamics have been widely shown to play a role in enzyme catalysis; however, the interplay between substrate dynamics and enzymatic activity is less understood. We report insights into the r...

journal_title：Biophysical journal

authors： Mercadante D,Melton LD,Jameson GB,Williams MA,De Simone A

更新日期：2013-04-16 00:00:00

abstract：:The conformation of mononucleosome complexes reconstituted with recombinant core histones on a 614-basepair-long DNA fragment containing the Xenopus borealis 5S rRNA nucleosome positioning sequence was studied by scanning/atomic force microscopy in the absence or presence of linker histone H1. Imaging without prior fi...

journal_title：Biophysical journal

authors： Kepert JF,Tóth KF,Caudron M,Mücke N,Langowski J,Rippe K

更新日期：2003-12-01 00:00:00

abstract：:The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause p...

journal_title：Biophysical journal

authors： Goll J,Carlson FD,Barenholz Y,Litman BJ,Thompson TE

更新日期：1982-04-01 00:00:00

abstract：:Fibrillar aggregation of the protein ataxin-3 is linked to the inherited neurodegenerative disorder Spinocerebellar ataxia type 3, a member of the polyQ expansion disease family. We previously reported that aggregation and stability of the nonpathological form of ataxin-3, carrying an unexpanded polyQ tract, are modul...

journal_title：Biophysical journal

authors： Masino L,Nicastro G,De Simone A,Calder L,Molloy J,Pastore A

更新日期：2011-04-20 00:00:00

abstract：:Protein-mediated DNA looping, such as that induced by the lactose repressor (LacI) of Escherichia coli, is a well-known gene regulation mechanism. Although researchers have given considerable attention to DNA looping by LacI, many unanswered questions about this mechanism, including the role of protein flexibility, re...

journal_title：Biophysical journal

authors： Hirsh AD,Lillian TD,Lionberger TA,Perkins NC

更新日期：2011-08-03 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:A brief summary of the major results in enhanced wound healing by electrolysis in animals and humans is presented along with the results of enhanced growth by electrolysis in plants. Hypotheses of normal and enhanced wound healing in animal and plants are reviewed. A comparison of the experimental results indicates st...

journal_title：Biophysical journal

authors： Gensler W

更新日期：1979-09-01 00:00:00

abstract：:Using glycerinated spasmoneme of giant Zoothamnium sp., the physical properties of spasmoneme before and after Ca2+-induced contraction (pCa 4.5) were investigated. The volume change of spasmoneme contraction was measured under zero tension. The length and diameter decreased by about 50% of their initial value as a re...

journal_title：Biophysical journal

authors： Moriyama Y,Okamoto H,Asai H

更新日期：1999-02-01 00:00:00

abstract：:Interactions between the graft copolymer poly(L-lysine)-g-poly(ethylene glycol), PLL-g-PEG, and two kinds of surface-supported lipidic systems (supported phospholipid bilayers and supported vesicular layers) were investigated by a combination of microscopic and spectroscopic techniques. It was found that the applicati...

journal_title：Biophysical journal

authors： Rossetti FF,Reviakine I,Csúcs G,Assi F,Vörös J,Textor M

更新日期：2004-09-01 00:00:00

abstract：:A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we suggest that this motif maximizes information flow in a noisy network. Gene expressio...

journal_title：Biophysical journal

authors： Hormoz S

更新日期：2013-03-05 00:00:00

abstract：:It is shown that alpha-helical content of eleven proteins is well correlated with alanine plus leucine content. These residues, taken singly or together, are to a first approximation randomly distributed in the four proteins whose tertiary structures have been determined (i.e., myoglobin, lysozyme, ribonuclease, alpha...

journal_title：Biophysical journal

authors： Prothero JW

更新日期：1968-11-01 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:We investigated the accessibility to protons and thiol-directed reagents of a cysteine substituted at position 338 in transmembrane segment 6 (TM6) of CFTR to test the hypothesis that T338 resides in the pore. Xenopus oocytes expressing T338C CFTR exhibited pH-dependent changes in gCl and I-V shape that were specific ...

journal_title：Biophysical journal

authors： Liu X,Zhang ZR,Fuller MD,Billingsley J,McCarty NA,Dawson DC

更新日期：2004-12-01 00:00:00

abstract：:Crystallographic structure and deuterium accessibility comparisons of CcO in different redox states have suggested conformational changes of mechanistic significance. To predict the intrinsic flexibility and low energy motions in CcO, this work has analyzed available high-resolution crystallographic structures with Pr...

journal_title：Biophysical journal

authors： Buhrow L,Ferguson-Miller S,Kuhn LA

更新日期：2012-05-02 00:00:00

abstract：:The partition coefficients (K(P)) of a series of single-chain and double-chain fluorescent amphiphiles, between solid ordered (P(beta') and L(beta)) and liquid disordered (L(alpha) of the type l(d)) lipid phases coexisting in the same lipid bilayer, was studied using steady-state fluorescence emission anisotropy. The ...

journal_title：Biophysical journal

authors： Mesquita RM,Melo E,Thompson TE,Vaz WL

更新日期：2000-06-01 00:00:00

abstract：:We have established a quantum mechanics (QM)/molecular mechanics (MM) hybrid method for calculating the Raman spectra of protein-bound cofactors using the alpha-subunit of C-phycocyanin containing a phycocyanobilin (PCB) chromophore as a test case. The PCB cofactor was described with density functional theory, whereas...

journal_title：Biophysical journal

authors： Mroginski MA,Mark F,Thiel W,Hildebrandt P

更新日期：2007-09-15 00:00:00

abstract：:Kinesin-1 is an ATP-driven molecular motor that transports cellular cargo along microtubules. At low loads, kinesin-1 almost always steps forward, toward microtubule plus ends, but at higher loads, it can also step backward. Backsteps are usually 8 nm but can be larger. These larger backward events of 16 nm, 24 nm, or...

journal_title：Biophysical journal

authors： Toleikis A,Carter NJ,Cross RA

更新日期：2020-11-17 00:00:00

abstract：:Cytoplasmic free Mg(2+) concentration ([Mg(2+)]i) was measured in rat ventricular myocytes with a fluorescent indicator furaptra (mag-fura-2) introduced by AM-loading. By incubation of the cells in a high-K(+) (Ca(2+)- and Mg(2+)-free) solution, [Mg(2+)]i decreased from ? 0.9 mM to 0.2 to 0.5 mM. The lowered [Mg(2+)]i...

journal_title：Biophysical journal

authors： Tashiro M,Inoue H,Konishi M

更新日期：2014-11-04 00:00:00