Abstract:
:The primary drivers of yeast endocytosis are actin polymerization and curvature-generating proteins, such as clathrin and BAR domain proteins. Previous work has indicated that these factors may not be capable of generating the forces necessary to overcome turgor pressure. Thus local reduction of the turgor pressure, via localized accumulation or activation of solute channels, might facilitate endocytosis. The possible reduction in turgor pressure was calculated numerically, by solving the diffusion equation through a Legendre polynomial expansion. It was found that for a region of increased permeability having radius 45 nm, as few as 60 channels with a spacing of 10 nm could locally decrease the turgor pressure by 50%. We identified a key dimensionless parameter, p = P1a/D, where P1 is the increased permeability, a is the radius of the permeable region, and D is the solute diffusion coefficient. When p > 0.44, the turgor pressure is locally reduced by >50%. An approximate analytic theory was used to generate explicit formulas for the turgor pressure reduction in terms of key parameters. These findings may also be relevant to plants, where the mechanisms that allow endocytosis to proceed despite high turgor pressure are largely unknown.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Scher-Zagier JK,Carlsson AEdoi
10.1016/j.bpj.2016.10.040subject
Has Abstractpub_date
2016-12-20 00:00:00pages
2747-2756issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(16)31000-1journal_volume
111pub_type
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