Pericellular Brush and Mechanics of Guinea Pig Fibroblast Cells Studied with AFM.

abstract：:A novel system for measuring, simultaneously, ciliary beating and intracellular free calcium is presented. The advantages and dynamic nature of the system are demonstrated by measuring the effects of the calcium ionophore lonomycin and of extracellular ATP on ciliated rabbit trachea. The results are discussed with reg...

journal_title：Biophysical journal

authors： Korngreen A,Priel Z

更新日期：1994-07-01 00:00:00

abstract：:Structures of truncated versions of the influenza A virus M2 proton channel have been determined recently by x-ray crystallography in the open conformation of the channel, and by NMR in the closed state. The structures differ in the position of the bound inhibitors. The x-ray structure shows a single amantadine molecu...

journal_title：Biophysical journal

authors： Chuang GY,Kozakov D,Brenke R,Beglov D,Guarnieri F,Vajda S

更新日期：2009-11-18 00:00:00

abstract：:This study presents a simplified version of the quasi-one-dimensional theory (Wu, J., E. A. Johnson, and J. M. Kootsey. 1996. A quasi-one-dimensional theory for anisotropic propagation of excitation in cardiac muscle. Biophys. J. 71:2427-2439) with two components of the extracellular current, along and perpendicular t...

journal_title：Biophysical journal

authors： Wu J,Wikswo JP Jr

更新日期：1997-11-01 00:00:00

abstract：:More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have...

journal_title：Biophysical journal

authors： Kasas S,Ikai A

更新日期：1995-05-01 00:00:00

abstract：:A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death...

journal_title：Biophysical journal

authors： Bell GI,Anderson EC

更新日期：1967-07-01 00:00:00

abstract：:A fundamental question concerning the ClC Cl-/H+ antiporters is the nature of their proton transport (PT) pathway. We addressed this issue by using a novel computational methodology capable of describing the explicit PT dynamics in the ClC-ec1 protein. The main result is that the Glu203 residue delivers a proton from ...

journal_title：Biophysical journal

authors： Wang D,Voth GA

更新日期：2009-07-08 00:00:00

abstract：:Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backb...

journal_title：Biophysical journal

authors： Mak CH,Phan ENH

更新日期：2018-05-08 00:00:00

abstract：:The prion protein (PrP), widely recognized to misfold into the causative agent of the transmissible spongiform encephalopathies, has previously been shown to bind to lipid membranes with binding influenced by both membrane composition and pH. Aside from the misfolding events associated with prion pathogenesis, PrP can...

journal_title：Biophysical journal

authors： Le Brun AP,Haigh CL,Drew SC,James M,Boland MP,Collins SJ

更新日期：2014-11-18 00:00:00

abstract：:The vibrational energy relaxation of a selected vibrational mode in cytochrome c--a C-D stretch in the terminal methyl group of Met80--has been studied using equilibrium molecular dynamics simulation and normal mode analysis methods. As demonstrated in the pioneering work of Romesberg and co-workers, isotopic labeling...

journal_title：Biophysical journal

authors： Bu L,Straub JE

更新日期：2003-09-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a sensitive and widely used technique for measuring diffusion. FCS data are conventionally modeled with a finite number of diffusing components and fit with a least-square fitting algorithm. This approach is inadequate for analyzing data obtained from highly heterogeneous...

journal_title：Biophysical journal

authors： Sengupta P,Garai K,Balaji J,Periasamy N,Maiti S

更新日期：2003-03-01 00:00:00

abstract：:The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

journal_title：Biophysical journal

authors： Berdiev BK,Latorre R,Benos DJ,Ismailov II

更新日期：2001-05-01 00:00:00

abstract：:The demembranated (skinned) muscle fiber preparation is widely used to investigate muscle contraction because the intracellular ionic conditions can be precisely controlled. However, plasma membrane removal results in a loss of osmotic regulation, causing abnormal hydration of the myofilament lattice and its proteins....

journal_title：Biophysical journal

authors： Tanner BC,Farman GP,Irving TC,Maughan DW,Palmer BM,Miller MS

更新日期：2012-09-19 00:00:00

abstract：:Interactions of membrane anchored molecules such as glycolipids with a membrane surface are important in determining headgroup conformation. It is therefore essential to represent these membrane surface interactions in molecular modeling studies of glycolipids and other membrane bound molecules. We introduce here an e...

journal_title：Biophysical journal

authors： Ram P,Kim E,Thomson DS,Howard KP,Prestegard JH

更新日期：1992-12-01 00:00:00

abstract：:Genomics is a sequence-based informatics science and a three-dimensional-structure-based material science. However, in practice, most genomics researchers utilize sequence-based informatics approaches or three-dimensional-structure-based material science techniques, not both. This division is, at least in part, the re...

journal_title：Biophysical journal

authors： Li Z,Sun R,Bishop TC

更新日期：2020-05-05 00:00:00

abstract：:Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid droplets. We apply Raman spectroscopy to investigate freezing of lipid droplets inside cumulus-ooc...

journal_title：Biophysical journal

authors： Okotrub KA,Mokrousova VI,Amstislavsky SY,Surovtsev NV

更新日期：2018-08-07 00:00:00

abstract：:Although significant progress has been made toward understanding morphogen-mediated patterning in development, control of the size and shape of tissues via local and global signaling is poorly understood. In particular, little is known about how cell-cell interactions are involved in the control of tissue size. The Hi...

journal_title：Biophysical journal

authors： Gou J,Lin L,Othmer HG

更新日期：2018-08-21 00:00:00

abstract：:Actin, a highly conserved cytoskeletal protein found in all eukaryotic cells, facilitates cell motility and membrane remodeling via a directional polymerization cycle referred to as treadmilling. The nucleotide bound at the core of each actin subunit regulates this process. Although the biochemical kinetics of treadmi...

journal_title：Biophysical journal

authors： Saunders MG,Tempkin J,Weare J,Dinner AR,Roux B,Voth GA

更新日期：2014-04-15 00:00:00

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Voltage gated potassium channels open and inactivate in response to changes of the voltage across the membrane. After removal of the fast N-type inactivation, voltage gated Shaker K-channels (Shaker-IR) are still able to inactivate through a poorly understood closure of the ion conduction pore. This, usually slower, i...

journal_title：Biophysical journal

authors： Oliva C,González V,Naranjo D

更新日期：2005-08-01 00:00:00

abstract：:Rearrangement of tertiary structure in response to mechanical force (termed tertiary structure elasticity) in the tandem Ig chain is the first mode of elastic response for muscle protein titin. Tertiary structure elasticity occurs at low stretching forces (few tens of pN), and was described at atomic resolution in a r...

journal_title：Biophysical journal

authors： Hsin J,Schulten K

更新日期：2011-02-16 00:00:00

abstract：:The use of one-dimensional electron density strip models in interpreting low-angle X-ray data from planar and concentric multilayered structures is described. Diffraction formulas for an n-strip model are given. Fourier transforms, normalization constants, and Patterson functions are derived for certain strip models. ...

journal_title：Biophysical journal

authors： Worthington CR

更新日期：1969-02-01 00:00:00

abstract：:Toward quantitative description of protein aggregation, we develop a computationally efficient method to evaluate the potential of mean force between two folded protein molecules that allows for complete sampling of their mutual orientation. Our model is valid at moderate ionic strengths and accounts for the actual ch...

journal_title：Biophysical journal

authors： Chan HY,Lankevich V,Vekilov PG,Lubchenko V

更新日期：2012-04-18 00:00:00

abstract：:The spontaneous folding of two Neisseria outer membrane proteins, opacity-associated (Opa)(60) and Opa(50) into lipid vesicles was investigated by systematically varying bulk and membrane properties. Centrifugal fractionation coupled with sodium dodecyl sulfate polyacrylamide gel electrophoresis mobility assays enable...

journal_title：Biophysical journal

authors： Dewald AH,Hodges JC,Columbus L

更新日期：2011-05-04 00:00:00

abstract：:Some membrane peptides, such as Alamethicin, form barrel-stave aggregates with a broad probability distribution of size (number of peptides in the aggregate). This distribution has been shown to depend on the characteristics of the lipid bilayer. A mechanism for this influence is suggested, in analogy to earlier work ...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2002-05-01 00:00:00

abstract：:Bacterial swimming speed is sometimes known to increase with viscosity. This phenomenon is peculiar to bacterial motion. Berg and Turner (Nature. 278:349-351, 1979) indicated that the phenomenon was caused by a loose, quasi-rigid network formed by polymer molecules that were added to increase viscosity. We mathematica...

journal_title：Biophysical journal

authors： Magariyama Y,Kudo S

更新日期：2002-08-01 00:00:00

abstract：:Pavlovian conditioning of the nudibranch mollusc Hermissenda crassicornis was previously shown to produce long-lasting reduction of two K+ currents measured across the Type B photoreceptor soma membrane (Alkon et al., 1982a; Alkon et al., 1985). Pavlovian conditioning of the rabbit was also shown to be followed by per...

journal_title：Biophysical journal

authors： Collin C,Ikeno H,Harrigan JF,Lederhendler I,Alkon DL

更新日期：1988-11-01 00:00:00

abstract：:A beat-to-beat variation in the cardiac action potential duration is a phenomenon known as alternans. Alternans has been linked to ventricular fibrillation, and thus the ability to predict the onset of alternans could be clinically beneficial. Theoretically, it has been proposed that the slope of a restitution curve, ...

journal_title：Biophysical journal

authors： Cram AR,Rao HM,Tolkacheva EG

更新日期：2011-02-16 00:00:00

abstract：:The aggregation of amyloid-β (Aβ) peptides plays a crucial role in the etiology of Alzheimer's disease (AD). Recently, it has been reported that an A2T mutation in Aβ can protect against AD. Interestingly, a nonpolar A2V mutation also has been found to offer protection against AD in the heterozygous state, although it...

journal_title：Biophysical journal

authors： Das P,Murray B,Belfort G

更新日期：2015-02-03 00:00:00

abstract：:More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders...

journal_title：Biophysical journal

authors： Garcia-Mira MM,Sanchez-Ruiz JM

更新日期：2001-12-01 00:00:00

abstract：:The absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. To meet this challenge we present an approach to construct a convex space, termed the k-cone, which c...

journal_title：Biophysical journal

authors： Famili I,Mahadevan R,Palsson BO

更新日期：2005-03-01 00:00:00