Computer modelling of glycolipids at membrane surfaces.

abstract：:Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...

journal_title：Biophysical journal

authors： Oelz DB,Del Castillo U,Gelfand VI,Mogilner A

更新日期：2018-10-16 00:00:00

abstract：:Theories of DNA electrophoretic separations generally treat the DNA as a free draining polymer moving in an electric field at a rate that depends on the effective charge density of the molecule. Separations can occur in sieving media ranging from ultradilute polymer solutions to tightly cross-linked gels. It has recen...

journal_title：Biophysical journal

authors： Ferree S,Blanch HW

更新日期：2004-07-01 00:00:00

abstract：:The rotation of human red blood cells (RBC) as they flow in the shear field established by a Coulter type orifice is modeled. This model, based on hydrodynamics of ellipsoid rotation in laminar creeping flow, is used to calculate the probability of the cells entering the orifice with a specific orientation. The electr...

journal_title：Biophysical journal

authors： Breitmeyer MO,Lightfoot EN,Dennis WH

更新日期：1971-02-01 00:00:00

abstract：:A variety of amphiphilic helical peptides have been shown to exhibit a transition from adsorbing parallel to a membrane surface at low concentrations to inserting perpendicularly into the membrane at high concentrations. Furthermore, this transition has been correlated to the peptides' cytolytic activities. X-ray lame...

journal_title：Biophysical journal

authors： Wu Y,He K,Ludtke SJ,Huang HW

更新日期：1995-06-01 00:00:00

abstract：:Recent theoretical work on the cooperative equilibrium binding of myosin subfragment-1-ADP to regulated actin, as influenced by Ca2+, is extended here to the cooperative steady-state ATPase activity of myosin subfragment-1 on regulated actin. Exact solution of the general steady-state problem will require Monte Carlo ...

journal_title：Biophysical journal

authors： Hill TL,Eisenberg E,Chalovich JM

更新日期：1981-07-01 00:00:00

abstract：:An attempt was made to confirm previous reports of resonant-like dielectric absorption of plasmid DNA in aqueous solutions at 1-10 GHz. The dielectric properties of the sample were measured using an automatic network analyzer with two different techniques. One technique used an open-ended coaxial probe immersed in the...

journal_title：Biophysical journal

authors： Foster KR,Epstein BR,Gealt MA

更新日期：1987-09-01 00:00:00

abstract：:To understand better the effect of electrostatics on the rigidity of the DNA double helix, we define DNA*, the null isomer of DNA, as the hypothetical structure that would result from DNA if its phosphate groups were not ionized. For the purposes of theoretical analysis, we model DNA* as identical to ordinary DNA but ...

journal_title：Biophysical journal

authors： Manning GS

更新日期：2006-11-15 00:00:00

abstract：:Intramolecular electron transfer between CuA and heme a in solubilized bacterial (Paracoccus denitrificans) cytochrome c oxidase was investigated by pulse radiolysis. CuA, the initial electron acceptor, was reduced by 1-methylnicotinamide radicals in a diffusion-controlled reaction, as monitored by absorption changes ...

journal_title：Biophysical journal

authors： Farver O,Grell E,Ludwig B,Michel H,Pecht I

更新日期：2006-03-15 00:00:00

abstract：:The orientational order as determined by 2H NMR and the infrared frequencies of the C--H stretching modes of the methylene groups have been measured for several systems (POPC, POPC/cholesterol and POPE), all in the fluid phase, and then were compared; this work reveals an unexpected linear correlation between them. Th...

journal_title：Biophysical journal

authors： Kodati VR,Lafleur M

更新日期：1993-01-01 00:00:00

abstract：:The ability to discriminate between different cations efficiently is essential for the proper physiological functioning of many membrane transport proteins. One obvious mechanism of ion selectivity is when a binding site is structurally constrained by the protein architecture and its geometry is precisely adapted to f...

journal_title：Biophysical journal

authors： Roux B

更新日期：2010-06-16 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

journal_title：Biophysical journal

authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

更新日期：1990-06-01 00:00:00

abstract：:An interactive program is described for calculating the second virial coefficient contribution to the thermodynamic nonideality of solutions of rigid macromolecules based on their triaxial dimensions. The FORTRAN-77 program, available in precompiled form for the PC, is based on theory for the covolume of triaxial elli...

journal_title：Biophysical journal

authors： Harding SE,Horton JC,Jones S,Thornton JM,Winzor DJ

更新日期：1999-05-01 00:00:00

abstract：:TOK1 encodes the channel responsible for the prominent outward K(+) current of the yeast plasma membrane. It can dwell in several impermeable states, including a rapidly transiting, K(+)-electromotive-force-dependent "R" (rectifying) state, a voltage-independent "IB" (interburst) state, and a set of [K(+)](ext) and vo...

journal_title：Biophysical journal

authors： Loukin SH,Saimi Y

更新日期：2002-02-01 00:00:00

abstract：:Extraction from simulations of the area compressibility moduli of the monolayers in a bilayer is considered theoretically. A statistical mechanical derivation shows that the bilayer modulus is the sum of the two monolayer moduli, as is often supposed but contrary to a recent study. Seemingly plausible assumptions rega...

journal_title：Biophysical journal

authors： Nagle JF

更新日期：2019-09-17 00:00:00

abstract：:The lateral mobility of the neural cell adhesion molecule (NCAM) was examined using single particle tracking (SPT). Various isoforms of human NCAM, differing in their ectodomain, their membrane anchorage mode, or the size of their cytoplasmic domain, were expressed in National Institutes of Health 3T3 cells and C2C12 ...

journal_title：Biophysical journal

authors： Simson R,Yang B,Moore SE,Doherty P,Walsh FS,Jacobson KA

更新日期：1998-01-01 00:00:00

abstract：:Coarse-grained molecular dynamics simulations are applied to explore the experimentally observed ability of the liquid-ordered (lo)/liquid-disordered (ld) phase boundary to facilitate viral membrane fusion. Surprisingly, a formed fusion stalk can be both attracted (i.e., stalkophilic) and repelled (i.e., stalkophobic)...

journal_title：Biophysical journal

authors： Risselada HJ

更新日期：2017-06-20 00:00:00

abstract：:Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding...

journal_title：Biophysical journal

authors： Salerno D,Beretta GL,Zanchetta G,Brioschi S,Cristofalo M,Missana N,Nardo L,Cassina V,Tempestini A,Giovannoni R,Cerrito MG,Zaffaroni N,Bellini T,Mantegazza F

更新日期：2016-05-24 00:00:00

abstract：:Biophysical understanding of membrane domains requires accurate knowledge of their structural details and elasticity. We report on a global small angle x-ray scattering data analysis technique for coexisting liquid-ordered (Lo) and liquid-disordered (Ld) domains in fully hydrated multilamellar vesicles. This enabled t...

journal_title：Biophysical journal

authors： Heftberger P,Kollmitzer B,Rieder AA,Amenitsch H,Pabst G

更新日期：2015-02-17 00:00:00

abstract：:Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Sing...

journal_title：Biophysical journal

authors： Olans L,Sariban-Sohraby S,Benos DJ

更新日期：1984-12-01 00:00:00

abstract：:The field of computational biology has been revolutionized by recent advances in genomics. The completion of a number of genome projects, including that of the human genome, has paved the way toward a variety of challenges and opportunities in bioinformatics and biological systems engineering. One of the first challen...

journal_title：Biophysical journal

authors： Klepeis JL,Floudas CA

更新日期：2003-10-01 00:00:00

abstract：:This paper describes the relation between the quantum yield of photosynthesis in the red alga Porphyridium cruentum, and the spectral composition of light, changed by filtering white light through aqueous phycobilin solutions of increasing optical density. At sufficiently high densities of the filter solution, no meas...

journal_title：Biophysical journal

authors： THOMAS JB,GOVINDJEE

更新日期：1960-09-01 00:00:00

abstract：:We measured the influence of alternative versions of the Drosophila melanogaster myosin heavy chain relay domain on muscle mechanical properties. We exchanged relay domain regions (encoded by alternative versions of exon 9) between an embryonic (EMB) isoform and the indirect flight muscle isoform (IFI) of myosin. Prev...

journal_title：Biophysical journal

authors： Yang C,Ramanath S,Kronert WA,Bernstein SI,Maughan DW,Swank DM

更新日期：2008-12-01 00:00:00

abstract：:Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in...

journal_title：Biophysical journal

authors： Mohan A,Schlue KT,Kniffin AF,Mayer CR,Duke AA,Narayanan V,Arsenovic PT,Bathula K,Danielsson BE,Dumbali SP,Maruthamuthu V,Conway DE

更新日期：2018-09-04 00:00:00

abstract：:A complete mechanistic understanding of membrane-localized processes in vesicular transport, such as membrane budding and fission, requires their reconstitution with biochemically-defined components from a biochemically-defined substrate. Supported bilayers formed by vesicle fusion represent an attractive substrate fo...

journal_title：Biophysical journal

authors： Pucadyil TJ,Schmid SL

更新日期：2010-07-21 00:00:00

abstract：:Experimental studies have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high repetition rate lasers, a phenomenon we call "light quenching." We now describe the theory of light quenching and some of its effects on the steady-state and time-resolved intensity and ani...

journal_title：Biophysical journal

authors： Kuśba J,Bogdanov V,Gryczynski I,Lakowicz JR

更新日期：1994-11-01 00:00:00

abstract：:The elastic theory of flexural waves in thin rods accurately predicts the velocity of flagellar bending waves over a wide range of viscosities. This shows that flagella behave as a purely mechanical system for the transmission of these waves. An evaluation of the total bending moment reveals that this moment occurs in...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1971-05-01 00:00:00

abstract：:The cell membrane of Valonia utricularis contains an electrogenic carrier system for chloride (Wang et al., Biophys J. 59:235-248 (1991)). The electrical impedance of V. utricularis was measured in the frequency range between 1 Hz and 50 kHz. The analysis of the impedance spectra from V. utricularis and its comparison...

journal_title：Biophysical journal

authors： Wang J,Zimmermann U,Benz R

更新日期：1994-10-01 00:00:00

abstract：:Two T = 1 and one T = 3 plant viruses, along with a protein, were crystallized in microgravity during the International Microgravity Laboratory-2 (IML-2) mission in July of 1994. The method used was liquid-liquid diffusion in the European Space Agency's Advanced Protein Crystallization Facility (APCF). Distinctive alt...

journal_title：Biophysical journal

authors： Koszelak S,Day J,Leja C,Cudney R,McPherson A

更新日期：1995-07-01 00:00:00

abstract：:We report the first time-resolved study of the two-dimensional x-ray diffraction pattern during active contraction in insect flight muscle (IFM). Activation of demembranated Lethocerus IFM was triggered by 1.5-2.5% step stretches (risetime 10 ms; held for 1.5 s) giving delayed active tension that peaked at 100-200 ms....

journal_title：Biophysical journal

authors： Tregear RT,Edwards RJ,Irving TC,Poole KJ,Reedy MC,Schmitz H,Towns-Andrews E,Reedy MK

更新日期：1998-03-01 00:00:00