Phospholipid Chain Interactions with Cholesterol Drive Domain Formation in Lipid Membranes.

abstract：:The formulation of Lorente de Nó for the potential distribution in a volume conductor due to a contained bioelectric source is reviewed. The aforementioned formula is based on data taken from the bioelectric source in air (excised). This work is extended to the derivation of a formula which depends upon quantities ava...

journal_title：Biophysical journal

authors： PLONSEY R

更新日期：1964-07-01 00:00:00

abstract：:This work builds upon the record-breaking speed and generous immediate release of new experimental 3D structures of the SARS-CoV-2 proteins and complexes, which are crucial to downstream vaccine and drug development. We have surveyed those structures to catch the occasional errors that could be significant for those i...

journal_title：Biophysical journal

authors： Croll TI,Williams CJ,Chen VB,Richardson DC,Richardson JS

更新日期：2021-01-15 00:00:00

abstract：:The orientational order as determined by 2H NMR and the infrared frequencies of the C--H stretching modes of the methylene groups have been measured for several systems (POPC, POPC/cholesterol and POPE), all in the fluid phase, and then were compared; this work reveals an unexpected linear correlation between them. Th...

journal_title：Biophysical journal

authors： Kodati VR,Lafleur M

更新日期：1993-01-01 00:00:00

abstract：:We have studied the kinetics of N epsilon-fluorescein isothiocyanate-lysine-23 cobra alpha-toxin (FITC-toxin) binding to the membrane-associated acetylcholine receptor from the Torpedo californica electric organ. The fluorescent toxin not only enabled us to monitor the binding reaction continuously but also to examine...

journal_title：Biophysical journal

authors： Cheung AT,Johnson DA,Taylor P

更新日期：1984-02-01 00:00:00

abstract：:The conformational states of Escherichia coli Rep helicase undergoing ATP hydrolysis while bound to a partial-duplex DNA (pdDNA) were studied using single-molecule FRET. Crystallographic studies showed that Rep bound to single-stranded DNA can exist in open and closed conformations that differ in the orientation of th...

journal_title：Biophysical journal

authors： Balci H,Arslan S,Myong S,Lohman TM,Ha T

更新日期：2011-08-17 00:00:00

abstract：:The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by ...

journal_title：Biophysical journal

authors： Mattila K,Kinder R,Bechinger B

更新日期：1999-10-01 00:00:00

abstract：:Certain antibiotic peptides are thought to permeabilize membranes of pathogens by effects that are also observed for simple detergents, such as membrane thinning and disordering, asymmetric bilayer expansion, toroidal pore formation, and micellization. Here we test the hypothesis that such peptides act additively with...

journal_title：Biophysical journal

authors： Patel H,Huynh Q,Bärlehner D,Heerklotz H

更新日期：2014-05-20 00:00:00

abstract：:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

journal_title：Biophysical journal

authors： Dusenbery DB,Uretz RB

更新日期：1972-08-01 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:Describing and understanding the biological function of a protein requires a detailed structural and thermodynamic description of the protein's native state ensemble. Obtaining such a description often involves characterizing equilibrium fluctuations that occur beyond the nanosecond timescale. Capturing such fluctuati...

journal_title：Biophysical journal

authors： Shehu A,Kavraki LE,Clementi C

更新日期：2007-03-01 00:00:00

abstract：:Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding...

journal_title：Biophysical journal

authors： Salerno D,Beretta GL,Zanchetta G,Brioschi S,Cristofalo M,Missana N,Nardo L,Cassina V,Tempestini A,Giovannoni R,Cerrito MG,Zaffaroni N,Bellini T,Mantegazza F

更新日期：2016-05-24 00:00:00

abstract：:In this article, optimization-based frameworks are introduced for elucidating the input-output structure of signaling networks and for pinpointing targeted disruptions leading to the silencing of undesirable outputs in therapeutic interventions. The frameworks are demonstrated on a large-scale reconstruction of a sign...

journal_title：Biophysical journal

authors： Dasika MS,Burgard A,Maranas CD

更新日期：2006-07-01 00:00:00

abstract：:Topical microbicide products are being developed for the prevention of sexually transmitted infections. These include vaginally-applied gels that deliver anti-HIV molecules. Gels may also provide partial barriers that slow virion diffusion from semen to vulnerable epithelium, increasing the time during which anti-HIV ...

journal_title：Biophysical journal

authors： Lai BE,Henderson MH,Peters JJ,Walmer DK,Katz DF

更新日期：2009-11-04 00:00:00

abstract：:The Qx-Qy splitting observed in the fluorescence excitation spectra of Mg-mesoporphyrin-IX substituted horseradish peroxidase (MgMP-HRP) and of its complex with naphthohydroxamic acid (NHA) was studied by spectral hole burning techniques. The width of a hole directly burnt in the Qy band and that of a satellite hole i...

journal_title：Biophysical journal

authors： Suisalu A,Mauring K,Kikas J,Herenyi L,Fidy J

更新日期：2001-01-01 00:00:00

abstract：:The effects of phosphorylation on the voltage-dependent properties of dihydropyridine-sensitive Ca channels of skeletal muscle were studied. Single channel currents were recorded upon incorporation of transverse tubule membranes into planar bilayers that were kept polarized at near physiological resting potential and ...

journal_title：Biophysical journal

authors： Mundiña-Weilenmann C,Ma J,Ríos E,Hosey MM

更新日期：1991-10-01 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:The vibrational Raman spectra of both pure 1-alpha-dimyristoylphosphatidic acid (DMPA) liposomes and DMPA multilayers reconstituted with ferricytochrome c at pH 7 and pH 4, with either sodium or calcium as the cation, are reported as a function of temperature. Multilayers composed of a 1:1 mol ratio DMPA and dimyristo...

journal_title：Biophysical journal

authors： Vincent JS,Levin IW

更新日期：1991-05-01 00:00:00

abstract：:Photosystem II catalyzes photosynthetic water oxidation in plants, green algae, and cyanobacteria. The manganese-containing active site cycles through a series of five oxidation states, S(n), where n refers to the number of oxidizing equivalents stored. In this report, reaction-induced Fourier transform infrared and e...

journal_title：Biophysical journal

authors： Halverson KM,Barry BA

更新日期：2003-10-01 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00

abstract：:Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

journal_title：Biophysical journal

authors： Sun C,Munn LL

更新日期：2005-03-01 00:00:00

abstract：:Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with superresolution axial sensitivity and no need for image analysis. We sh...

journal_title：Biophysical journal

authors： Cade NI,Fruhwirth G,Archibald SJ,Ng T,Richards D

更新日期：2010-06-02 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:The mechanosensitive channel of large conductance (MscL) belongs to a family of transmembrane channel proteins in bacteria and functions as a safety valve that relieves the turgor pressure produced by osmotic downshock. MscL gating can be triggered solely by stretching of the membrane. This work reports an effort to u...

journal_title：Biophysical journal

authors： Jeon J,Voth GA

更新日期：2008-05-01 00:00:00

abstract：:We developed a model of the rat ventricular myocyte at room temperature to predict the relative effects of different mechanisms on the cause of the slow increase in force in response to a step change in muscle length. We performed simulations in the presence of stretch-dependent increases in flux through the Na(+)-H(+...

journal_title：Biophysical journal

authors： Niederer SA,Smith NP

更新日期：2007-06-01 00:00:00

abstract：:Myxicola giant axons internally injected with tetraethylammonium chloride to block potassium currents were examined under voltage clamp. The sodium inactivation time constants obtained from the decline in INa during step depolarizations were substantially smaller than those obtained using conditioning prepulses to the...

journal_title：Biophysical journal

authors： Schauf CL,Pencek TL,Davis FA

更新日期：1976-09-01 00:00:00

abstract：:Hormones are released from neuroendocrine cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. An elegant way to study this process at the single-vesicle level is to use styryl dyes, which stain not only the membrane, but also the matrix of individual vesicles in some neuroe...

journal_title：Biophysical journal

authors： Stenovec M,Poberaj I,Kreft M,Zorec R

更新日期：2005-04-01 00:00:00

abstract：:Mechanosensitive ion channels (MSCs) which could provide for fast osmoregulatory responses in bacteria, remain unidentified as molecular entities. MSCs from Escherichia coli (strain AW740) were examined using the patch-clamp technique, either (a) in giant spheroplasts, (b) after reconstitution by fusing native membran...

journal_title：Biophysical journal

authors： Sukharev SI,Martinac B,Arshavsky VY,Kung C

更新日期：1993-07-01 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:Plasma insulin measurements from mice, rats, dogs, and humans indicate that insulin levels are oscillatory, reflecting pulsatile insulin secretion from individual islets. An unanswered question, however, is how the activity of a population of islets is coordinated to yield coherent oscillations in plasma insulin. Here...

journal_title：Biophysical journal

authors： Fendler B,Zhang M,Satin L,Bertram R

更新日期：2009-08-05 00:00:00