Assignment of segments of the bacteriorhodopsin sequence to positions in the structural map.

abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...

journal_title：Biophysical journal

authors： Jelcic M,Enyedi B,Xavier JB,Niethammer P

更新日期：2017-05-09 00:00:00

abstract：:The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...

journal_title：Biophysical journal

authors： Malewicz B,Valiyaveettil JT,Jacob K,Byun HS,Mattjus P,Baumann WJ,Bittman R,Brown RE

更新日期：2005-04-01 00:00:00

abstract：:The temporal dynamics of the concentrations of several proteins are tightly regulated, particularly for critical nodes in biological networks such as transcription factors. An important mechanism to control transcription factor levels is through autoregulatory feedback loops where the protein can bind its own promoter...

journal_title：Biophysical journal

authors： Murugan R,Kreiman G

更新日期：2011-09-21 00:00:00

abstract：:The efficiency of various patterns of pulsatile stimulation is determined in a model in which a receptor becomes desensitized in the presence of its stimulatory ligand. The effect of stochastic or chaotic changes in the duration and/or interval between successive pulses in a series of square-wave stimuli is investigat...

journal_title：Biophysical journal

authors： Li Y,Goldbeter A

更新日期：1992-01-01 00:00:00

abstract：:The rat connexin40 gap junction channel is permeable to monovalent cations including tetramethylammonium and tetraethylammonium ions. Larger tetraalkyammonium (TAA(+)) ions beginning with tetrabutylammonium (TBA(+)) reduced KCl junctional currents disproportionately. Ionic blockade by tetrapentylammonium (TPeA(+)) and...

journal_title：Biophysical journal

authors： Musa H,Gough JD,Lees WJ,Veenstra RD

更新日期：2001-12-01 00:00:00

abstract：:The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular co...

journal_title：Biophysical journal

authors： Tursun P,Tashiro M,Konishi M

更新日期：2005-03-01 00:00:00

abstract：:Methods are described for discrimination of models of the gating kinetics and permeation of single ionic channels. Both maximum likelihood and regression procedures are discussed. In simple situations, where models are nested, standard hypothesis tests can be used. More commonly, however, non-nested models are of inte...

journal_title：Biophysical journal

authors： Horn R

更新日期：1987-02-01 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:Time courses of myelin lattice swelling in toad sciatic nerves preexposed to different treatments were determined by x-ray diffraction using a one-dimensional position-sensitive detector. In the nerves supramaximally stimulated for 1 h at 200 Hz, the subsequent process of myelin swelling occurred 45.0 +/- 7.3 min (n =...

journal_title：Biophysical journal

authors： Padrón R,Mateu L

更新日期：1982-08-01 00:00:00

abstract：:In this article, we explore the information content of molecular force-field calculations. We make use of exhaustive lattice models of molecular conformations and reduced alphabet sequences to determine the relative resolving power of pairwise interaction-based force fields. We find that sequence-specific interactions...

journal_title：Biophysical journal

authors： Aynechi T,Kuntz ID

更新日期：2005-11-01 00:00:00

abstract：:Current viral docking models have relied upon the assumption that bond formation and breakage are independent of viral and docking surface geometry, as well as the forces exerted on the bonds. This assumption, known as the equivalent site hypothesis (ESH), is examined in detail using a newly developed simulation techn...

journal_title：Biophysical journal

authors： English TJ,Hammer DA

更新日期：2004-06-01 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backb...

journal_title：Biophysical journal

authors： Mak CH,Phan ENH

更新日期：2018-05-08 00:00:00

abstract：:A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including (13)C and (15)N chemical shift anisotropies and (13)C-(15)N dipolar coupl...

journal_title：Biophysical journal

authors： Holt A,Rougier L,Réat V,Jolibois F,Saurel O,Czaplicki J,Killian JA,Milon A

更新日期：2010-05-19 00:00:00

abstract：:We have constructed a theory for diffusion through the pore of a single-ion channel by taking a limit of a random walk around a cycle of states. Similar to Levitt's theory of single-ion diffusion, one obtains boundary conditions for the Nernst-Planck equation that guarantee that the pore is occupied by at most one ion...

journal_title：Biophysical journal

authors： McGill P,Schumaker MF

更新日期：1996-10-01 00:00:00

abstract：:The bonding rules for actin filament bundles do not lead to a particular packing symmetry, but allow for either regular or disordered filament packing. Indeed, both hexagonal and disordered types of packing are observed in vivo. To investigate factors which control bundle order, as well as size, we examined the effect...

journal_title：Biophysical journal

authors： Stokes DL,DeRosier DJ

更新日期：1991-02-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:The structure and biophysical properties of lipid membranes are important for cellular functions in health and disease. In Alzheimer's disease, the neuronal membrane is a target for toxic amyloid-β (Aβ). Melatonin is an important pineal gland hormone that has been shown to protect against Aβ toxicity in cellular and a...

journal_title：Biophysical journal

authors： Mei N,Robinson M,Davis JH,Leonenko Z

更新日期：2020-12-15 00:00:00

abstract：:Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for the influence of the wall shear stress on the early-stage adhesion of Pseudomonas aeruginosa PA14 on glass and polydimethylsiloxane surfaces. We use image analysis to measure the residence time o...

journal_title：Biophysical journal

authors： Lecuyer S,Rusconi R,Shen Y,Forsyth A,Vlamakis H,Kolter R,Stone HA

更新日期：2011-01-19 00:00:00

abstract：:Enterococcus hirae V1-ATPase is a molecular motor composed of the A3B3 hexamer ring and the central stalk. In association with ATP hydrolysis, three catalytic AB pairs in the A3B3 ring undergo conformational changes, which lead to a 120° rotation of the central stalk. To understand how the conformational changes of th...

journal_title：Biophysical journal

authors： Isaka Y,Ekimoto T,Kokabu Y,Yamato I,Murata T,Ikeguchi M

更新日期：2017-03-14 00:00:00

abstract：:We introduce an intrinsically multiplexed and easy to implement method to apply an external force to a biomolecule and thus probe its interaction with a second biomolecule or, more generally, its environment (for example, the cell membrane). We take advantage of the hydrodynamic interaction with a controlled fluid flo...

journal_title：Biophysical journal

authors： Türkcan S,Richly MU,Bouzigues CI,Allain JM,Alexandrou A

更新日期：2013-07-02 00:00:00

abstract：:To understand the thermodynamic exclusion of Na(+) relative to K(+) from the S(2) site of the selectivity filter, the distribution P(X)(epsilon) (X = K(+) or Na(+)) of the binding energy (epsilon) of the ion with the channel is analyzed using the potential distribution theorem. By expressing the excess chemical potent...

journal_title：Biophysical journal

authors： Dixit PD,Merchant S,Asthagiri D

更新日期：2009-03-18 00:00:00

abstract：:The parameters of the immune response dynamics are usually estimated by the use of deterministic ordinary differential equations that relate data trends to parameter values. Since the physical basis of the response is stochastic, we are investigating the intensity of the data fluctuations resulting from the intrinsic ...

journal_title：Biophysical journal

authors： Milutinovic D,De Boer RJ

更新日期：2007-05-15 00:00:00

abstract：:Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective observation of coordinated spontaneous fluctuations in two separate detection channels that unambiguously reflect the existence o...

journal_title：Biophysical journal

authors： Heinze KG,Rarbach M,Jahnz M,Schwille P

更新日期：2002-09-01 00:00:00

abstract：:Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

journal_title：Biophysical journal

authors： Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

更新日期：2009-02-18 00:00:00

abstract：:Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin g...

journal_title：Biophysical journal

authors： Horn C,Steinem C

更新日期：2005-08-01 00:00:00

abstract：:Halobacterium halobium contains at least three retinal-containing pigments: bacteriorhodopsin, halorhodopsin, and a third rhodopsin-like pigment (tR) absorbing at approximately 590 nm, tR590. Illumination of tR590 gives rise to a very long-lived blue absorbing photoproduct, tR370. Using high-performance liquid chromat...

journal_title：Biophysical journal

authors： Tsuda M,Nelson B,Chang CH,Govindjee R,Ebrey TG

更新日期：1985-05-01 00:00:00

abstract：:Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitu...

journal_title：Biophysical journal

authors： Murovec T,Sweeney DC,Latouche E,Davalos RV,Brosseau C

更新日期：2016-11-15 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:The type 2a sarco/endoplasmic reticulum (ER) Ca2+-ATPase (SERCA2a) plays a key role in intracellular Ca2+ regulation in the heart. We have previously shown evidence of stable homodimers of SERCA2a in heterologous cells and cardiomyocytes. However, the functional significance of the pump dimerization remains unclear. H...

journal_title：Biophysical journal

authors： Bovo E,Nikolaienko R,Cleary SR,Seflova J,Kahn D,Robia SL,Zima AV

更新日期：2020-10-06 00:00:00