Dimerization of SERCA2a Enhances Transport Rate and Improves Energetic Efficiency in Living Cells.

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:The dependences of the steady-state critical concentration and average filament length of actin solutions, on the filament branching and capping rates, are calculated using a rate methodology based on the total number of actin filaments. The methodology generalizes calculations of the "treadmilling" actin concentratio...

journal_title：Biophysical journal

authors： Carlsson AE

更新日期：2005-07-01 00:00:00

abstract：:Presented here is a quantitative model of the wrapping and unwrapping of the DNA around the histone core of the nucleosome that suggests a mechanism by which this transition can be controlled: alteration of the charge state of the globular histone core. The mechanism is relevant to several classes of posttranslational...

journal_title：Biophysical journal

authors： Fenley AT,Adams DA,Onufriev AV

更新日期：2010-09-08 00:00:00

abstract：:Activation of the photoreceptor G protein transducin (Gt) by opsin, the ligand-free form of rhodopsin, was measured using rod outer segment membranes with densities of opsin and Gt similar to those found in rod cells. When GTPgammaS was used as the activating nucleotide, opsin catalyzed transducin activation with an e...

journal_title：Biophysical journal

authors： Melia TJ Jr,Cowan CW,Angleson JK,Wensel TG

更新日期：1997-12-01 00:00:00

abstract：:Long time series of monosynaptic Ia-afferent to alpha-motoneuron reflexes were recorded in the L7 or S1 ventral roots in the cat. Time series were collected before and after spinalization at T13 during constant amplitude stimulations of group Ia muscle afferents in the triceps surae muscle nerves. Using autocorrelatio...

journal_title：Biophysical journal

authors： Chang T,Schiff SJ,Sauer T,Gossard JP,Burke RE

更新日期：1994-08-01 00:00:00

abstract：:Previously (Hines, M., and J.J. Blum 1983, Biophys. J., 41:67-79), a method was developed that allowed one to compute curvature and twist for a three-dimensional sliding filament model. In that formalism it was difficult to specify the shear and bending moments arising from moment-bearing interfilament links such as f...

journal_title：Biophysical journal

authors： Hines M,Blum JJ

更新日期：1984-11-01 00:00:00

abstract：:The molecular code of specific DNA recognition by proteins as a paradigm in molecular biology remains an unsolved puzzle primarily because of the subtle interplay between direct protein-DNA interaction and the indirect contribution from water and ions. Transformation of the nonspecific, low affinity complex to a speci...

journal_title：Biophysical journal

authors： Fuxreiter M,Mezei M,Simon I,Osman R

更新日期：2005-08-01 00:00:00

abstract：:Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as ...

journal_title：Biophysical journal

authors： Atanasov V,Knorr N,Duran RS,Ingebrandt S,Offenhäusser A,Knoll W,Köper I

更新日期：2005-09-01 00:00:00

abstract：:Oriented solid-state NMR in combination with multiple-residue-specific (15)N labeling and extensive numerical spectral analysis is proposed to determine helix conformations of large membrane proteins in native membranes. The method is demonstrated on uniaxially oriented samples of (15)N-methionine, -valine, and -glyci...

journal_title：Biophysical journal

authors： Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

更新日期：2008-01-01 00:00:00

abstract：:In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially ...

journal_title：Biophysical journal

authors： Greiss F,Deligiannaki M,Jung C,Gaul U,Braun D

更新日期：2016-02-23 00:00:00

abstract：:The S4 segments of voltage-gated sodium channels are important parts of the voltage-sensing elements of these proteins. Furthermore, the addition of the isolated S4 polypeptide to planar lipid bilayers results in stepwise increases of ion conductivity. In order to gain insight into the mechanisms of pore formation by ...

journal_title：Biophysical journal

authors： Mattila K,Kinder R,Bechinger B

更新日期：1999-10-01 00:00:00

abstract：:In the budding yeast nucleus, transfer RNA (tRNA) genes are considered to localize in the vicinity of the nucleolus; however, the use of Hi-C and fluorescent repressor-operator system techniques has clearly indicated that the tRNA genes are distributed not only around the nucleolus but also at other nuclear locations....

journal_title：Biophysical journal

authors： Tokuda N

更新日期：2020-05-05 00:00:00

abstract：:Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

journal_title：Biophysical journal

authors： Slochower DR,Gilson MK

更新日期：2018-05-08 00:00:00

abstract：:Extraction from simulations of the area compressibility moduli of the monolayers in a bilayer is considered theoretically. A statistical mechanical derivation shows that the bilayer modulus is the sum of the two monolayer moduli, as is often supposed but contrary to a recent study. Seemingly plausible assumptions rega...

journal_title：Biophysical journal

authors： Nagle JF

更新日期：2019-09-17 00:00:00

abstract：:We report that long-chain poly-L-glutamine forms cation-selective channels when incorporated into artificial planar lipid bilayer membranes. The channel was permeable to alkali cations and H(+) ions and virtually impermeable to anions; the selectivity sequence based on the single-channel conductance was H(+) > Cs(+) >...

journal_title：Biophysical journal

authors： Monoi H,Futaki S,Kugimiya S,Minakata H,Yoshihara K

更新日期：2000-06-01 00:00:00

abstract：:The kinetic properties of the three taxonomic A substates of sperm whale carbonmonoxy myoglobin in 75% glycerol/buffer are studied by flash photolysis with monitoring in the infrared stretch bands of bound CO at nu(A0) approximately 1967 cm-1, nu(A1) approximately 1947 cm-1, and nu(A3) approximately 1929 cm-1 between ...

journal_title：Biophysical journal

authors： Johnson JB,Lamb DC,Frauenfelder H,Müller JD,McMahon B,Nienhaus GU,Young RD

更新日期：1996-09-01 00:00:00

abstract：:We investigate a temperature-driven recursive division of binary giant unilamellar vesicles (GUVs). During the heating step of the heating-cooling cycle, the spherical mother vesicle deforms to a budded limiting shape using up the excess area produced by the chain melting of the lipids and then splits off into two dau...

journal_title：Biophysical journal

authors： Jimbo T,Sakuma Y,Urakami N,Ziherl P,Imai M

更新日期：2016-04-12 00:00:00

abstract：:To assess if membrane diffusion could affect the kinetics of receptor recruitment at adhesive contacts, we transfected neurons with green fluorescent protein-tagged immunoglobin cell adhesion molecules of varying length (25-180 kD), and measured the lateral mobility of single quantum dots bound to those receptors at t...

journal_title：Biophysical journal

authors： Thoumine O,Saint-Michel E,Dequidt C,Falk J,Rudge R,Galli T,Faivre-Sarrailh C,Choquet D

更新日期：2005-11-01 00:00:00

abstract：:Phosphatidylinositol-4,5-bisphosphate (PIP2) is an important signaling lipid in eukaryotic cell plasma membranes, playing an essential role in diverse cellular processes. The headgroup of PIP2 is highly negatively charged, and this lipid displays a high critical micellar concentration compared to housekeeping phosphol...

journal_title：Biophysical journal

authors： Shukla S,Jin R,Robustelli J,Zimmerman ZE,Baumgart T

更新日期：2019-09-03 00:00:00

abstract：:Molecular chaperone Hsp70 plays important roles in the pathology of amyloid diseases by inhibiting aberrant aggregation of proteins. However, the biophysical mechanism of the interaction of Hsp70 with the intrinsically disordered proteins (IDPs) is unclear. Here, we report that Hsp70 inhibits aggregation of islet amyl...

journal_title：Biophysical journal

authors： Chilukoti N,Sil TB,Sahoo B,Deepa S,Cherakara S,Maddheshiya M,Garai K

更新日期：2021-01-06 00:00:00

abstract：:In the photocycle of bacteriorhodopsin (bR) from Halobacterium salinarum mutant L93A, the O-intermediate accumulates and the cycling time is increased approximately 200 times. Nevertheless, under continuous illumination, the protein pumps protons at near wild-type rates. We excited the mutant L93A in purple membrane w...

journal_title：Biophysical journal

authors： Tóth-Boconádi R,Keszthelyi L,Stoeckenius W

更新日期：2003-06-01 00:00:00

abstract：:Ionic channels bathed in mixed solutions of two permeant electrolytes often conduct less current than channels bathed in pure solutions of either. For many years, this anomalous mole fraction effect (AMFE) has been thought to occur only in single-file pores containing two or more ions at a time. Most thinking about ch...

journal_title：Biophysical journal

authors： Nonner W,Chen DP,Eisenberg B

更新日期：1998-05-01 00:00:00

abstract：:In a relaxed muscle fiber at low ionic strength, the cross-bridges may well be in states comparable to the one that precedes the cross-bridge power stroke (Schoenberg, M. 1988. Adv. Exp. Med. Biol. 226:189-202). Using electron paramagnetic resonance (EPR) and (saturation transfer) electron paramagnetic resonance (ST-E...

journal_title：Biophysical journal

authors： Fajer PG,Fajer EA,Schoenberg M,Thomas DD

更新日期：1991-09-01 00:00:00

abstract：:We investigated whether the cysteine residues in Shaker potassium (K+) channels are essential for activation and inactivation gating or for modulation of activation gating by external zinc (Zn2+). Mutants of the Shaker K+ channel were prepared in which all seven cysteine residues were replaced (C-less). These changes ...

journal_title：Biophysical journal

authors： Boland LM,Jurman ME,Yellen G

更新日期：1994-03-01 00:00:00

abstract：:The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable...

journal_title：Biophysical journal

authors： Sawetzki T,Eggleton CD,Desai SA,Marr DW

更新日期：2013-11-19 00:00:00

abstract：:Pure bending of a membrane bilayer is developed including different properties for each membrane half. Both connected and unconnected bilayer surfaces are treated. The bilayer bending resistance is the resultant of parallel surface compression "resistances." The neutral surface is a function of the upper and lower sur...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1974-12-01 00:00:00

abstract：:Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

journal_title：Biophysical journal

authors： Hampp N,Bräuchle C,Oesterhelt D

更新日期：1990-07-01 00:00:00

abstract：:The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular co...

journal_title：Biophysical journal

authors： Tursun P,Tashiro M,Konishi M

更新日期：2005-03-01 00:00:00

abstract：:Nonbleachable rhodopsins containing retinal moieties with fixed 11-ene structures have been prepared. When the nonbleachable rhodopsin analogue corresponding to the natural pigment was flash-photolysed at 20.8 degrees C, no absorption changes occurred at the monitoring wavelengths of 380, 480, and 580 nm for the time ...

journal_title：Biophysical journal

authors： Mao B,Tsuda M,Ebrey TG,Akita H,Balogh-Nair V,Nakanishi K

更新日期：1981-08-01 00:00:00

abstract：:The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential ...

journal_title：Biophysical journal

authors： Everts S,Davis JH

更新日期：2000-08-01 00:00:00