Orientational disorder and motion of weakly attached cross-bridges.

abstract：:Theoretical studies of the electronic structure and spectra of models for the ferric resting state and Compound I intermediates of horseradish peroxidase (HRP-I) and catalase (CAT-I) have been performed using the INDO-RHF/CI method. The goals of these studies were twofold: i) to determine whether the axial ligand of H...

journal_title：Biophysical journal

authors： Du P,Loew GH

更新日期：1995-01-01 00:00:00

abstract：:Water plays an important role in enzyme structure and function in aqueous media. That role becomes even more important when one focuses on enzymes in low water media. Here we present results from molecular dynamics simulations of surfactant-solubilized subtilisin BPN' in three organic solvents (octane, tetrahydrofuran...

journal_title：Biophysical journal

authors： Yang L,Dordick JS,Garde S

更新日期：2004-08-01 00:00:00

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:Mitochondria often reside in subcellular regions with high metabolic demands. We examined the mechanisms that can govern the relocation of mitochondria to these sites in respiratory neurons. Mitochondria were visualized using tetramethylrhodamineethylester, and their movements were analyzed by applying single-particle...

journal_title：Biophysical journal

authors： Mironov SL

更新日期：2007-04-15 00:00:00

abstract：:The active amplification of sound-induced vibrations in the cochlea, known to be crucial for auditory sensitivity and frequency selectivity, is not well understood. The outer hair cell (OHC) somatic electromotility is a potential mechanism for such amplification. Its effectiveness in vivo is putatively limited by the ...

journal_title：Biophysical journal

authors： Ramamoorthy S,Nuttall AL

更新日期：2012-02-08 00:00:00

abstract：:Kinetic measurements of the self-assembly of proteins into amyloid fibrils are often used to make inferences about molecular mechanisms. In particular, the lag time--the quiescent period before aggregates are detected--is often found to scale with the protein concentration as a power law, whose exponent has been used ...

journal_title：Biophysical journal

authors： Eden K,Morris R,Gillam J,MacPhee CE,Allen RJ

更新日期：2015-02-03 00:00:00

abstract：:One of the unusual properties of elastin is its ability to coacervate, which has been proposed to play an important role in the alignment of monomeric elastin for cross-linking into the polymeric elastin matrix. The temperature at which this transition takes place depends on several factors including protein concentra...

journal_title：Biophysical journal

authors： Ostuni A,Bochicchio B,Armentano MF,Bisaccia F,Tamburro AM

更新日期：2007-11-15 00:00:00

abstract：:Synchrotron x-ray diffraction, differential scanning calorimetry, and electron spin resonance spectroscopy have been employed to characterize a quasicrystalline phase formed in aqueous dispersions of binary mixtures of glucocerebroside and palmitoyloleoylphosphatidylethanolamine. Small- and wide-angle x-ray scattering...

journal_title：Biophysical journal

authors： Feng Y,Rainteau D,Chachaty C,Yu ZW,Wolf C,Quinn PJ

更新日期：2004-04-01 00:00:00

abstract：:The cytoplasmic surface of the BR (initial) state of bacteriorhodopsin is characterized by a cluster of three carboxylates that function as a proton-collecting antenna. Systematic replacement of most of the surface carboxylates indicated that the cluster is made of D104, E161, and E234 (Checover, S., Y. Marantz, E. Na...

journal_title：Biophysical journal

authors： Nachliel E,Gutman M,Tittor J,Oesterhelt D

更新日期：2002-07-01 00:00:00

abstract：:Stabilized intermediate redox states of cytochrome c are generated by radiolytic reduction of initially oxidized enzyme in glass matrices at liquid nitrogen temperature. In the intermediate states the heme group is reduced by hydrated electrons, whereas the protein conformation is restrained close to its oxidized form...

journal_title：Biophysical journal

authors： Cartling B

更新日期：1983-08-01 00:00:00

abstract：:Integrin-mediated signaling is crucial for cell-substrate adhesion and can be triggered from both intra- and extracellular interactions. Although talin binding is sufficient for inside-out activation of integrin, other cytoplasmic proteins such as α-actinin and filamin can directly interfere with talin-mediated integr...

journal_title：Biophysical journal

authors： Shams H,Mofrad MRK

更新日期：2017-08-22 00:00:00

abstract：:Structure-function studies of the Cys loop family of ionotropic neurotransmitter receptors (GABA, nACh, 5-HT(3), and glycine receptors) have resulted in a six-loop (A-F) model of the agonist-binding site. Key amino acids have been identified in these loops that associate with, and stabilize, bound ligand. The next ste...

journal_title：Biophysical journal

authors： Khatri A,Sedelnikova A,Weiss DS

更新日期：2009-01-01 00:00:00

abstract：:In this study maximum shortening velocity (Vmax) and isometric tension (P0) in skinned single fibers from rat slow soleus (SOL) and fast superficial vastus lateralis (SVL) muscles were examined after varying degrees of filament lattice compression with dextran. In both fiber types Vmax was greatest in the absence of d...

journal_title：Biophysical journal

authors： Metzger JM,Moss RL

更新日期：1987-07-01 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:Differentiation from a multipotent stem or progenitor state to a mature cell is an essentially irreversible process. The associated changes in gene expression patterns exhibit time-directionality. This "arrow of time" in the collective change of gene expression across multiple stable gene expression patterns (attracto...

journal_title：Biophysical journal

authors： Wang J,Xu L,Wang E,Huang S

更新日期：2010-07-07 00:00:00

abstract：:Understanding the fundamentals of genome packaging in viral capsids is important for finding effective antiviral strategies and for utilizing benign viral particles for gene therapy. While the structure of encapsidated genomic materials has been routinely characterized with experimental techniques such as cryo-electro...

journal_title：Biophysical journal

authors： Kim J,Wu J

更新日期：2015-10-20 00:00:00

abstract：:Detection algorithms are widely used for the analysis of single-channel data because they remove the background noise from the measured current signal and reconstruct the noise-free time series. Standard detection algorithms assume channels switching only between zero and full conductance. Many types of channels, howe...

journal_title：Biophysical journal

authors： Draber S,Schultze R

更新日期：1994-10-01 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:Structural transition can be induced in charged micelles by increasing the ionic strength of the medium. We have monitored the organization and dynamics of the functionally important tryptophan residues of gramicidin in spherical and rod-shaped sodium dodecyl sulfate micelles utilizing a combination of wavelength-sele...

journal_title：Biophysical journal

authors： Rawat SS,Kelkar DA,Chattopadhyay A

更新日期：2005-11-01 00:00:00

abstract：:Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particle...

journal_title：Biophysical journal

authors： Brown PH,Schuck P

更新日期：2006-06-15 00:00:00

abstract：:Cyclic nucleotide-gated (CNG) channels, which were initially studied in retina and olfactory neurons, are activated by cytoplasmic cGMP or cAMP. Detailed comparisons of nucleotide-activated currents using nucleotide analogs and mutagenesis revealed channel-specific residues in the nucleotide-binding domain that regula...

journal_title：Biophysical journal

authors： Scott SP,Cummings J,Joe JC,Tanaka JC

更新日期：2000-05-01 00:00:00

abstract：:DNA beacons are short single-stranded chains which can form closed hairpin shapes through complementary base pairing at their ends. Contrary to the common polymer theory assumption that only their loop length matters, experiments show that their closing kinetics depend on the loop composition. We have modeled the clos...

journal_title：Biophysical journal

authors： Aalberts DP,Parman JM,Goddard NL

更新日期：2003-05-01 00:00:00

abstract：:Missense mutations in the membrane-binding actin-based motor protein, myosin-1a (Myo1a), have recently been linked to sensorineural deafness in humans. One of these mutations, E385D, impacts a residue in the switch II region of the motor domain that is present in virtually all members of the myosin superfamily. We sou...

journal_title：Biophysical journal

authors： Yengo CM,Ananthanarayanan SK,Brosey CA,Mao S,Tyska MJ

更新日期：2008-01-15 00:00:00

abstract：:Morphogenesis of epithelial tissues requires tight spatiotemporal coordination of cell shape changes. In vivo, many tissue-scale shape changes are driven by pulsatile contractions of intercellular junctions, which are rectified to produce irreversible deformations. The functional role of this pulsatory ratchet and its...

journal_title：Biophysical journal

authors： Staddon MF,Cavanaugh KE,Munro EM,Gardel ML,Banerjee S

更新日期：2019-11-05 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:The elastic properties of cylindrical segments of sarcolemma were studied in single striated fibers of the frog semitendinosus muscle. All measurements were made on membranes of retraction zones, cell segments from which the sarcoplasm had retracted. Quantitative morphological studies indicated that three deforming fo...

journal_title：Biophysical journal

authors： Fields RW

更新日期：1970-05-01 00:00:00

abstract：:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier ...

journal_title：Biophysical journal

authors： Dusenbery DB,Uretz RB

更新日期：1972-08-01 00:00:00

abstract：:Keratin intermediate filaments form dynamic intracellular networks, which span the entire cytoplasm and provide mechanical strength to the cell. The mechanical resilience of the keratin intermediate filament network itself is determined by filament bundling. The bundling process can be reproduced in artificial conditi...

journal_title：Biophysical journal

authors： Haimov E,Windoffer R,Leube RE,Urbakh M,Kozlov MM

更新日期：2020-07-07 00:00:00

abstract：:Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligo...

journal_title：Biophysical journal

authors： Pannwitt S,Stangl M,Schneider D

更新日期：2019-11-05 00:00:00

abstract：:Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spa...

journal_title：Biophysical journal

authors： Selhuber-Unkel C,Erdmann T,López-García M,Kessler H,Schwarz US,Spatz JP

更新日期：2010-02-17 00:00:00