Stationary and nonstationary correlation-frequency analysis of heterodyne mode laser light scattering: magnitude and periodicity of canine tracheal ciliary beat frequency in vivo.


:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was used to assess the magnitude of the CBF stimulatory responses induced by aerosolized 10(-5) M fenoterol (sympathomimetic), and 10(-8) M and 10(-6) M methacholine (parasympathomimetic) delivered to the whole lungs of eight barbiturate-anesthetized beagles. The nonstationary correlation-frequency analysis laser light-scattering technique was used to measure the effect on tracheal CBF of increasing the cytosolic calcium ion concentration with a calcium ionophore, A23187. Aerosolized A23187 was delivered to the isolated tracheal lumens of eight beagle dogs in cumulative doses ranging from 10(-9)M to 10(-6) M. Administration of the ionophore synchronized the CBF with a period of 5.3 min. Dose dependencies were observed in both the time to the peak CBF stimulation and the magnitude of the stimulatory response. The magnitude of CBF stimulation was inhibited by prior administration of aerosolized nifedipine (2 mg/ml), a voltage-operated calcium channel blocker. The A23187-induced modulation period of tracheal CBF, was unchanged by nifedipine. These are the first data to demonstrate that the magnitude and periodicity of CBF are two independent coupled processes. The cooperativity of these two processes could be determined in the effectiveness of mucociliary transport.


Biophys J


Biophysical journal


Chandra T,Yeates DB,Miller IF,Wong LB




Has Abstract


1994-03-01 00:00:00




3 Pt 1










  • Effect of fluorine substitution on the interaction of lipophilic ions with the plasma membrane of mammalian cells.

    abstract::The effects of the anionic tungsten carbonyl complex [W(CO)(5)SC(6)H(5)](-) and its fluorinated analog [W(CO)(5)SC(6)F(5)](-) on the electrical properties of the plasma membrane of mouse myeloma cells were studied by the single-cell electrorotation technique. At micromolar concentrations, both compounds gave rise to a...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kürschner M,Nielsen K,von Langen JR,Schenk WA,Zimmermann U,Sukhorukov VL

    更新日期:2000-09-01 00:00:00

  • Events leading to the opening and closing of the exocytotic fusion pore have markedly different temperature dependencies. Kinetic analysis of single fusion events in patch-clamped mouse mast cells.

    abstract::The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clampe...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Oberhauser AF,Monck JR,Fernandez JM

    更新日期:1992-03-01 00:00:00

  • Variable symmetry in Salmonella typhimurium flagellar motors.

    abstract::Electron cryomicroscopy of rotor complexes of the Salmonella typhimurium flagellar motor, overproduced in a nonmotile Escherichia coli host, has revealed a variation in subunit symmetry of the cytoplasmic ring (C ring) module. C rings with subunit symmetries ranging from 31 to 38 were found. They formed a Gaussian dis...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Young HS,Dang H,Lai Y,DeRosier DJ,Khan S

    更新日期:2003-01-01 00:00:00

  • Carcinoma and SV40-transfected normal ovarian surface epithelial cell comparison by nonphotochemical hole burning.

    abstract::Results are presented of nonphotochemical-hole-burning experiments on the mitochondrial specific dye rhodamine 800 incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV167 carcinoma cells. This dye is selective for the plasma and inner membranes of the mitochondria, as shown b...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Walsh RJ,Reinot T,Hayes JM,Kalli KR,Hartmann LC,Small GJ

    更新日期:2003-02-01 00:00:00

  • Geometry and energetics of DNA basepairs and triplets from first principles quantum molecular relaxations.

    abstract::A first principles model for calculating hydrogen bonding interactions, previously applied to water, is here applied to the more difficult problem of interactions between DNA bases. We first consider the energetics and geometry for the A-T and the G-C basepairs, comparing our results to other calculated results as wel...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lewis JP,Sankey OF

    更新日期:1995-09-01 00:00:00

  • Effects of hydrostatic pressure on bilayer phase behavior and dynamics of dilauroylphosphatidylcholine.

    abstract::Deuterium nuclear magnetic resonance spectroscopy was used to study the thermotropic phase behavior of dilauroylphosphatidylcholine (DLPC) bilayers at pressures up to 221 MPa. Pressure was found to separate the liquid crystal to gel transition from the gel to ordered crystalline phase transition. The jump in chain ord...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bonev B,Morrow MR

    更新日期:1996-06-01 00:00:00

  • Protein Partitioning into Ordered Membrane Domains: Insights from Simulations.

    abstract::Cellular membranes are laterally organized into domains of distinct structures and compositions by the differential interaction affinities between various membrane lipids and proteins. A prominent example of such structures are lipid rafts, which are ordered, tightly packed domains that have been widely implicated in ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lin X,Gorfe AA,Levental I

    更新日期:2018-04-24 00:00:00

  • Structure and activation dynamics of RBL-2H3 cells observed with scanning force microscopy.

    abstract::Surface and subsurface dynamics of Rat Basophilic Leukemia cells, a model system of stimulated secretion, were imaged using Scanning Force Microscopy (SFM) at a rate of 50-60 s/image. Cytoskeletal elements and organelles were tracked within quiescent cells and those activated after IgE receptor crosslinking. In additi...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Braunstein D,Spudich A

    更新日期:1994-05-01 00:00:00

  • Effects of monofunctional adducts of platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes.

    abstract::Effects of adducts of [PtCl(NH3)3]Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bursova V,Kasparkova J,Hofr C,Brabec V

    更新日期:2005-02-01 00:00:00

  • A modified cable formalism for modeling neuronal membranes at high frequencies.

    abstract::Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bédard C,Destexhe A

    更新日期:2008-02-15 00:00:00

  • Vesicle size distributions measured by flow field-flow fractionation coupled with multiangle light scattering.

    abstract::The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Korgel BA,van Zanten JH,Monbouquette HG

    更新日期:1998-06-01 00:00:00

  • Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.

    abstract::Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resol...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gustafsson MG,Shao L,Carlton PM,Wang CJ,Golubovskaya IN,Cande WZ,Agard DA,Sedat JW

    更新日期:2008-06-01 00:00:00

  • Intracellular calibration of the calcium indicator indo-1 in isolated fibers of Xenopus muscle.

    abstract::Estimates of the free myoplasmic [Ca2+] ([Ca2+]i) with fluorescent dyes are complicated by the fact that some properties of these dyes are altered in the intracellular environment. In the present study indo-1 was used to measure [Ca2+]i in isolated muscle fibers from Xenopus frogs. Fluorescent ratio signals obtained f...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Westerblad H,Allen DG

    更新日期:1996-08-01 00:00:00

  • Teaching biophysics. Strategies for recruiting and retaining minorities in physics and biophysics.

    abstract::Several strategies directed toward increasing the participation of minority students in physics and biophysics are presented. Since the number of minority students entering college with an interest in science and mathematics must be increased if we expect to see more students graduating in science, several programs ai...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tanaka JC,Gladney LD

    更新日期:1993-07-01 00:00:00

  • Coupling between lipid shape and membrane curvature.

    abstract::Using molecular dynamics simulations, we examine the behavior of lipids whose preferred curvature can be systematically varied. This curvature is imposed by controlling the headgroup size of a coarse-grained lipid model recently developed by us. To validate this approach, we examine self-assembly of each individual li...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cooke IR,Deserno M

    更新日期:2006-07-15 00:00:00

  • Simultaneous Determination of Two Subdomain Folding Rates Using the "Transfer-Quench" Method.

    abstract::The investigation of the mechanism of protein folding is complicated by the context dependence of the rates of intramolecular contact formation. Methods based on site-specific labeling and ultrafast spectroscopic detection of fluorescence signals were developed for monitoring the rates of individual subdomain folding ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Rahamim G,Amir D,Haas E

    更新日期:2017-05-09 00:00:00

  • Role of pairwise interactions between M1 and M2 domains of the nicotinic receptor in channel gating.

    abstract::The adult form of the nicotinic acetylcholine receptor (AChR) consists of five subunits (alpha(2)betaepsilondelta), each having four transmembrane domains (M1-M4). The atomic model of the nicotinic acetylcholine receptor shows that the pore-lining M2 domains make no extensive contacts with the rest of the transmembran...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Corradi J,Spitzmaul G,De Rosa MJ,Costabel M,Bouzat C

    更新日期:2007-01-01 00:00:00

  • Hydrodynamic properties of human erythrocyte band 3 solubilized in reduced Triton X-100.

    abstract::The oligomeric state and function of band 3, purified by sulfhydryl affinity chromatography in reduced Triton X-100, was investigated. Size exclusion high-performance liquid chromatography showed that a homogeneous population of band 3 dimers could be purified from whole erythrocyte membranes. The elution profile of b...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Taylor AM,Boulter J,Harding SE,Cölfen H,Watts A

    更新日期:1999-04-01 00:00:00

  • Propagation speed in myelinated nerve. I. Experimental dependence on external Na and on temperature.

    abstract::Conduction speed (theta) in single myelinated Rana pipiens sciatic nerve fibers has been precisely measured using intracellular recording and on-line digital computer techniques. The dependence of relative speed on external Na concentration at 15 degrees C has been found to be ln(theta(1)/theta(2)) = 0.524 (+/-0.018) ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hardy WL

    更新日期:1973-10-01 00:00:00

  • The thermodynamics of general anesthesia.

    abstract::It is known that the action of general anesthetics is proportional to their partition coefficient in lipid membranes (Meyer-Overton rule). This solubility is, however, directly related to the depression of the temperature of the melting transition found close to body temperature in biomembranes. We propose a thermodyn...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Heimburg T,Jackson AD

    更新日期:2007-05-01 00:00:00

  • Myosin cross-bridge kinetics and the mechanism of catch.

    abstract::Catch force in molluscan smooth muscle requires little, if any, energy input and is controlled by the phosphorylation state of the thick filament-associated mini-titin, twitchin. The kinetic parameters of myosin cross-bridge turnover in permeabilized catch muscle, and how they are potentially modified by the catch mec...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Franke AS,Mooers SU,Narayan SR,Siegman MJ,Butler TM

    更新日期:2007-07-15 00:00:00

  • Comparison of DPPC and DPPG environments in pulmonary surfactant models.

    abstract::Deuterium nuclear magnetic resonance was used to monitor lipid acyl-chain orientational order in suspensions of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) containing Ca(2+) and the lung surfactant proteins SP-A and SP-B separately and together. To distinguish between protein-lipid...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Morrow MR,Temple S,Stewart J,Keough KM

    更新日期:2007-07-01 00:00:00

  • Excitation transport and trapping on spectrally disordered lattices.

    abstract::It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jean JM,Chan CK,Fleming GR,Owens TG

    更新日期:1989-12-01 00:00:00

  • Experimental and computational studies investigating trehalose protection of HepG2 cells from palmitate-induced toxicity.

    abstract::Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Leekumjorn S,Wu Y,Sum AK,Chan C

    更新日期:2008-04-01 00:00:00

  • Ion competition in condensed DNA arrays in the attractive regime.

    abstract::Physical origin of DNA condensation by multivalent cations remains unsettled. Here, we report quantitative studies of how one DNA-condensing ion (Cobalt(3+) Hexammine, or Co(3+)Hex) and one nonDNA-condensing ion (Mg(2+)) compete within the interstitial space in spontaneously condensed DNA arrays. As the ion concentrat...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Qiu X,Giannini J,Howell SC,Xia Q,Ke F,Andresen K

    更新日期:2013-08-20 00:00:00

  • Role of macromolecular depletion in red blood cell adhesion.

    abstract::The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zhang ZW,Neu B

    更新日期:2009-08-19 00:00:00

  • Residue-specific α-helix propensities from molecular simulation.

    abstract::Formation of α-helices is a fundamental process in protein folding and assembly. By studying helix formation in molecular simulations of a series of alanine-based peptides, we obtain the temperature-dependent α-helix propensities of all 20 naturally occurring residues with two recent additive force fields, Amber ff03w...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Best RB,de Sancho D,Mittal J

    更新日期:2012-03-21 00:00:00

  • Reversible surface aggregation in pore formation by pardaxin.

    abstract::The mechanism of leakage induced by surface active peptides is not yet fully understood. To gain insight into the molecular events underlying this process, the leakage induced by the peptide pardaxin from phosphatidylcholine/ phosphatidylserine/cholesterol large unilamellar vesicles was studied by monitoring the rate ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Rapaport D,Peled R,Nir S,Shai Y

    更新日期:1996-06-01 00:00:00

  • Activation of skinned trabeculae of the guinea pig induced by laser photolysis of caged ATP.

    abstract::The kinetics of force production in chemically skinned trabeculae from the guinea pig were studied by laser photolysis of caged ATP in the presence of Ca2+. Preincubation of the tissue during rigor with the enzyme apyrase was used to reduce the population of MgADP-bound cross-bridges (Martin and Barsotti, 1994). In un...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Martin H,Barsotti RJ

    更新日期:1994-11-01 00:00:00

  • K-Ras4B Remains Monomeric on Membranes over a Wide Range of Surface Densities and Lipid Compositions.

    abstract::Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially the possibility that it may form dimers. In this article, we describ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Chung JK,Lee YK,Denson JP,Gillette WK,Alvarez S,Stephen AG,Groves JT

    更新日期:2018-01-09 00:00:00